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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
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Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 39, Issue 2 - Jun 2016
Volume 39, Issue 1 - Mar 2016
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Molecular identification of Bacillus licheniformis isolates from Korean traditional fermented soybean by the multilocus phylogenetic analysis
Moon, Sung-Hyun ; Hossain, Md Mukter ; Oh, Yeonsu ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 1~6
DOI : 10.7853/kjvs.2016.39.1.1
In this study, Bacillus licheniformis which has been used as probiotics was isolated from Korean traditional fermented soybean. A total of 69 strains were presumptively identified as B. licheniformis by phenotypic methods. Based on PCR amplification and 16S rRNA gene sequencing, the multilocus sequence typing of gyrA and rpoB, followed by phylogenetic analysis was performed. The isolates were distinctly differentiated and found to be closely related to B. amyloliquefaciens, B. subtilis, and B. aerius. The partial 16S rRNA gene sequences of those strains matched those of B. sonorensis (97%) and B. aerius (98%) in the phylogenetic tree. In contrast, multilocus phylogenetic analysis (MLPA) showed that only 61 (86.9%) out of 69 strains were B. licheniformis. The rest of those strains were found to be B. subtilis (5.8%), B. amyloliquefaciens (2.9%), and B. sonorensis (2.9%), respectively. Therefore, our results suggested that since the 16S rRNA gene sequencing alone was not sufficient to compare and discriminate closely related lineages of Bacillus spp., it was required to analyze the MLPA simultaneously to avoid any misleading phenotype-based grouping of these closely related species.
Prediction of ham weight with the autofom in Korea
Bae, Jin-Gyu ; Lee, Young-Kyu ; Park, Beom-Young ; Lym, Hyo-Seon ; Jung, Bong-Su ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 7~12
DOI : 10.7853/kjvs.2016.39.1.7
The Autofom is a equipment for predicting the amount of pig carcasses meat using the 16 ultrasonic sensors to measure in real time and it was established in Dodram LPC in Gyeonggi Province of Korea for the first time. This study was carried out to validate the reliability of Autofom statistically and to establish guideline for developing a analytic formula through comparing the measurement between Autofom and dissection. The ham parts of sixty-six pig carcasses were measured with Autofom and by two experimental performers. The weight means and standard deviations of ham parts including bone by measurements with Autofom and dissection were
, respectively a strong positive correlation (P<0.01) was identified, with a coefficient of determination (
) of 0.82. The weight means and standard deviations of lean ham parts by measurements with Autofom and dissection were
, respectively a strong positive correlation (P<0.01) was identified, with a coefficient of determination (
) of 0.72. The root mean square errors of two groups were 0.40 and 0.50, respectively.
Distribution status survey of livestock Products Sold via Online Websites
Kim, Ji Yeon ; Seo, Eun Ju ; Koh, Ba Ra Da ; Seo, Doo Ri ; Jung, Bo Ram ; Seo, Mi Hee ; Lim, Jin Teak ; Kim, Eun Sun ; Kim, Yong Hwan ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 13~20
DOI : 10.7853/kjvs.2016.39.1.13
This study is aimed to find out distribution status of online-market livestock products by purchasing and examining 120 cases of livestock products (seasoned meat: 17, 33 cases of packaged meat, 23 cases of ground meat, 19 cases of ham, 11 cases of sausage, 4 cases of bacon, 1 case of meat processing, 8 cases of Meat extract processed, and 4 cased of Dry storage of meat) at 17 On-line markets from April to August. 2015. We checked the weight of them first, and carried out ingredients test for each of processed meats. And we performed gene screening test on the products which were labelled `Hanwoo` to investigate that the products were made of Korean native cattle. we also carried out test of identifying domestic animal species on ham, sausage and ground processed products. After weighing all products, we could know that all of them were delivered more than labelled weight or in allowable error. The result values of test which measured level of preservatives, Nitrite, Volatile Basic Nitrite (VBN), and tar Color by the type of processed meat products were in permissible range or not detected. Also, 17 beefs inspected Korean native cattle gene test were confirmed that they were made by real korean native cattle. But 2 cases of Ham, sausage, and ground processed products had difference between label and goods. In this study, we could make a decision that livestock products, distributed in On-line markets, were safe and expect to make higher degrees of hygiene for livestock products seller. Futhermore, we hoped result of this study could be used by basic data for progressive national policy decisions.
Prevalence of Eimeria infection in commercial broilers in Gyeonggi province, Korea
Yang, Byung-Hun ; Shim, Hang-Sub ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 21~28
DOI : 10.7853/kjvs.2016.39.1.21
Protozoan parasite, the genus Eimeria, causes an intestinal disease, coccidiosis, in young broilers. Coccidiosis induces significant economic loss in poultry production. This study was designed to identify the prevalence of Eimeria spp. in broilers in Gyeonggi province, Korea. Fecal samples from broilers at 94 farms were collected in two chicken slaughterhouses between March and June 2015. Eimeria infection was determined by microscopic examination and PCR using ITS-1 region. The prevalence of Eimeria was 58.5% (55 of 94). E. acervulina was identified in 96.4% of samples, E. tenella in 81.8%, E. maxima in 12.7%, E. praecox in 12.7%, E. brunetti in 5.5%, E. necatrix in 1.8%, and E. mitis in 1.8%. Body weight (BW) of broilers infected with both E. tenella and E. acervulina ($mean
A survey for tick-borne pathogens in Korean native cattle from northern area of Gyeongbuk
Cho, Jae-Cheong ; Jeon, Woo-Jin ; Kim, Seon-Soo ; Kim, Seong-Guk ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 29~34
DOI : 10.7853/kjvs.2016.39.1.29
This study was carried out to investigate the tick-borne pathogens from one hundred nineteen cattle farms (38 farms of Andong, 41 of Yeongju, 12 of Uiseong, 5 of Cheongsong, 5 of Yoengyang, 18 of Bonghwa) in northern areas of Gyeongbuk province by polymerase chain reaction (PCR). Among 119 cattle farms, the positive ratios against Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia were 3.4% (4/119), 10.1% (12/119), 6.7% (8/119), 1.7% (2/119) and 16.8% (20/119), respectively. Also, the PCR results revealed that 8 farms were positive for T. sergenti in positive of Theileria and 2 farms were positive for A. phagocytophilum in positive of Anaplasma. Therefore, further studies regarding vectors, environmental condition, interaction between domestic and wild animals and development of control program are needed to reduce the numbers of bovine tick-borne disease in northern areas of Gyeongbuk province.
Detection ratio of bacterial and viral pathogens of diarrhea from Korean indigenous goat feces in Gyeongbuk province
Sohn, Jun-Hyung ; Do, Jae-Cheul ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 35~39
DOI : 10.7853/kjvs.2016.39.1.35
The purpose of this study was to survey on infection status of pathogens of diarrhea from Korean indigenous goat. A total of 800 fecal samples was collected from 50 farms from January to October 2015 and was tested by automatic biochemical machine and polymerase chain reaction (PCR). The overall detection ratio of bacterial pathogens was 22.4% and viral pathogens was 16.3%, respectively. The detection ratio of Escherichia coli (E. coli), Salmonella spp., bovine viral diarrhea virus (BVDV), rotavirus and coronavirus were 21.5%, 0.9%, 7.6%, 5.6% and 3.0%, respectively. In the rates of mixed detection, single was 78.2%, double 8.4%, triple 11.6% and quadruple 1.8% in each sample and 38%, 12%, 16%, 20% in each farm, respectively.
Application of next generation sequencing (NGS) system for whole-genome sequencing of porcine reproductive and respiratory syndrome virus (PRRSV)
Moon, Sung-Hyun ; Khatun, Amina ; Kim, Won-Il ; Hossain, Md Mukter ; Oh, Yeonsu ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 41~49
DOI : 10.7853/kjvs.2016.39.1.41
In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to maximum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which require the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.
Characteristics of antimicrobial resistant Salmonella Typhimurium isolates from chicken and pigs
Moon, Ja Young ; Kim, Won Kyong ; Lee, Kun Hee ; Na, Bang Ju ; Go, Jae Cheon ; Sim, Kyun Seob ; Trupti A, Patil ; Hur, Jin ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 51~57
DOI : 10.7853/kjvs.2016.39.1.51
Fifty and forty two Salmonella enterica subspecies enterica serovar 52 Typhimurium (ST) strains were isolated from chicken and pigs, respectively, collected from markets throughout Korea from 2008 to 2011. The isolates were investigated for the presence of antimicrobial resistance and multi-drug patterns. All 50 ST isolates from chicken and 42 ST isolates from pigs were resistant to at least one of 13 antibiotics used in this study, 92.0% of ST isolates from chicken and 88.1% of ST isolates from pigs were resistant to three or more antimicrobials. As many as 3 isolates of ST isolates from chicken were resistant to 11 of 13 antimicrobials tested in this study. Only one isolate of ST isolates from pigs was resistant to 10 of 13 antimicrobials. The ACSSuT resistance phenotype was observed in 34% of the 50 isolates and 23.8% of the 42 isolates. Especially, 1 isolate from pigs had the ACSSuTAu. The high rate of antimicrobial-resist and multi-drug resistant (MDR) ST isolation may give rise to crucial public health problems. Therefore, control of antimicrobial use, and continuous monitoring of antimicrobial resistance and MDR patterns among Salmonella isolates in chicken and pig farms is necessary to ensure public health.
Prevalence of infection and genetic analysis of duck circovirus in Jeonbuk province
Jeong, Han-Sol ; Baek, Kui-Jeong ; Koh, Won-Seok ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 59~63
DOI : 10.7853/kjvs.2016.39.1.59
Duck circovirus (DuCV) has been recognized as a contagious immunosuppressive virus affecting many duck species worldwide. To determine the prevalence of DuCV infection in ducks, we investigated 104 samples collected from 50 duck farms in Jeonbuk province with the polymerase chain reaction (PCR). Among collected samples, 40 (38.5%) were positive for DuCV. The prevalence of DuCV PCR-positive samples increased with age. Genomic sequences of 15 DuCV strains were determined and compared with previously available DuCV sequences in public databases. Phylogenetic analysis revealed that all strains were clustered into DuCV-1 group.
A case of submandibular pyogranuloma caused by Trueperella pyogenes in the slaughtered dairy cows
Jung, Ji-Youl ; Jeong, Jae-Hun ; Kim, Ha-Young ; O, Sang-Ik ; Ryu, Dae Yeol ; Yoon, Hyoung Lok ; So, ByungJae ; Yoon, Soon-Seek ;
Korean Journal of Veterinary Service, volume 39, issue 1, 2016, Pages 65~68
DOI : 10.7853/kjvs.2016.39.1.65
Multiple enlarged submandibular nodules were discovered during a routine antimortem examination at slaughter in 3 dairy cows of 20-month-old. Grossly, submandibular nodules were firm, reddish, and oval in shape. On cut surface, multiple yellowish abscesses in the parenchyma were observed. Histopathologically, the nodules were characterized by severe diffuse pyogranulomatous inflammation and chronic fibrosis with multiple eosinophilic clubs of Splendore-Hoeppli material containing central bacterial colonies. Trueperella pyogenes was isolated from the lesions. This case was diagnosed as submandibular pyogranuloma caused by Trueperella pyogenes in slaughtered dairy cows in Korea.