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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
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Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 39, Issue 2 - Jun 2016
Volume 39, Issue 1 - Mar 2016
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Research of body characteristics and behavioral patterns in Jeju Dogs
Oh, Myoung-Oun ; Park, Suk-Jae ; Bae, Jae Ho ; Kwon, Tae Jun ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 69~74
DOI : 10.7853/kjvs.2016.39.2.69
Jeju Dogs have high academic value due to their unique characteristics and conditions, but they have received little attention in the research field. This paper attempts to secure fundamental data to clarify the general characteristics of Jeju Dogs. For this purpose, an inspection of the basic body shape was performed to establish the shape and breed standards. Jeju Dogs were also exposed to 12 behavior tests to check their distinct character categories and an objective ethogram was built on each of the character categories to conduct a behavior analysis. The body shape of 34 clinically healthy Jeju Dogs aged 18 months or older was inspected using ten divided parts. For the behavior analysis, 10 Jeju Dogs were examined. A variety of behavioral variables were recorded based on 12 behavior tests that were categorized into four characters: sociality, aggressiveness, anxiety and submissiveness. The results of the body shape inspection indicated that most of the male dogs` parts had larger measured values than the female dogs` parts. The behavior analysis results revealed that the Jeju Dog displays excellent qualities such as submissiveness, reliability and fidelity and, hence, it is believed that this breed of dog is very appropriate for most duties and has the potential to be a household dog or working dog.
Distribution of ticks carrying Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) around Jiri walking trails of Jeollanam-do, Korea
Song, Byung Joon ; Lim, Hyun Cheol ; Ha, Tae Man ; Jeon, Doo Yung ; Yang, Soo In ; Song, Hyeon Je ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 75~80
DOI : 10.7853/kjvs.2016.39.2.75
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease characterized by fever and thrombocytopenia. Haemaphysalis longicornis ticks comprise the major population of ticks in the environment and have been considered as the main vector for SFTS virus (SFTSV). Here we investigated the distribution of ticks carrying SFTSV collected from the environment using the dragging or sweeping methods during April~October 2015 in Jeollanam-do, Korea. Sampling was taken from Songjeong, Omi, Bangkwang, Sandong areas in Jiri walking trails. Among the total 3,869 ticks collected, 3,823 ticks (98.8%) were H. longicornis, 41 (1.1%) were Amblyomma testudinarium, and 5 (0.1%) were Ixodes nipponensis. Classification results by regional groups of H. longicornis indicated that 1,613 ticks were collected in Sandong, 1,190 ticks in Omi, 603 ticks in Bangkwang, and 417 ticks in Songjeong. In monthly distributional studies of H. longicornis based on the developmental stages, nymph (325 ticks) was collected from May to October, 94% of larvae from April to June, and 94% of adult from June to August. These results showed the different dominant stage of ticks according to seasons. However, no SFTSV-specific gene was detected in 3,823 ticks of H. longicornis, 41 of A. testudinarium and 5 of I. nipponensis.
Experimental studies of homogeneity and stability honeybee venom using ultra-high performance liquid chromatography
Han, Sang Mi ; Kim, Se Gun ; Hong, In Phyo ; Woo, Soon Ok ; Jang, Hye Ri ; Lee, Kyung Woo ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 81~86
DOI : 10.7853/kjvs.2016.39.2.81
Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.
Evaluation of the virulence genes and Shiga toxin-producing abilities of Escherichia coli field isolates causing edema disease in pigs
Seo, Byoung-Joo ; Jeong, Chang-Gi ; Kang, A-Rum ; Cho, Ho-Seong ; Kim, Won-Il ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 87~92
DOI : 10.7853/kjvs.2016.39.2.87
Porcine edema disease (ED) is a communicable disease of pigs caused by infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) which expresses F18 fimbriae and/or Stx type 2e (Stx2e). While STEC causes a severe illness including hemorrhagic colitis and hemolytic-uremic syndrome in humans, it induces damage to the vascular endothelium, which results in edema, hemorrhage, and microthrombosis, leading in high mortality in pigs. In the present study, we cultured Stx2e-producing E. coli field isolates from conventional pig farms that experienced sudden deaths previously with symptoms similar to porcine edema disease, which were further investigated with Shiga toxin profiles. A total of 43 strains were identified from the collected samples by F18 or Stx2e specific PCR. Based on the PCR, 42 isolates out of 43 isolates were proved to carry one of F18 or Stx2e genes and 14 isolates to carry both F18 and Stx2e genes. All of the 30 isolates that harbored Stx2e gene induced the cytopathic effect (CPE) in vero cells and especially, the isolate 150229 produced the highest level of Shiga toxin. Therefore, we identified the virulence genes (F18 and Stx2e) and demonstrated Shiga toxin-producing abilities from porcine edema disease causing E. coli filed isolates. These results suggested that one of the isolates could be a vaccine antigen candidate against STEC through further investigating to elicit an immune response.
Effects of Xylazine or Acepromazine in dogs under constant rate infusion with alfaxalone
Yun, Sungho ; Kwon, Young-Sam ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 93~99
DOI : 10.7853/kjvs.2016.39.2.93
The anesthetic depth and cardiovascular effect of alfaxalone constant rate infusion in dogs premedicated with xylazine or acepromazine were evaluated. Ten dogs were randomly allocated into 2 groups. In group AA, dogs were premedicated with 0.02 mg/kg of intravenous acepromazine at 15 min before induction. In group XA 1.1 mg/kg of intravenous xylazine was premedicated at 5 min before induction. The anesthesia was maintained with 6 mg/kg/hr of alfaxalone after induction with 2 mg/kg alfaxalone in both groups. In both of groups, the qualities of induction were satisfactory without any adverse event, but adequate analgesia could not be provided, according to the withdrawal test.
implied a slight hypoxemia state in XA group, while those values of group AA were not significantly changed. The acepromazine and alfaxalone combination induce mild tachycardia. The bispectral index score were significantly decreased in group XA, compared with that in group AA. The premedication of xylazine before alfaxalone constant rate infusion in this study could provide adequate analgesia during 30 min, while the premedication with acepromazine could not.
Seroprevalence and molecular detection of Mycoplasma gallisepticum and M. synoviae infection in broiler breeder in Jeonbuk providence, Korea
Kwak, Kilhan ; Lee, Heungiae ; Yuk, Hyunsu ; Lee, Jaeuk ; Lee, Kwanho ; Yi, Young-Joo ; Lee, Sang-Myeong ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 101~105
DOI : 10.7853/kjvs.2016.39.2.101
The present study investigated serological and molecular prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) infection in unvaccinated broiler breeder farms in Jeonbuk providence. An enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) had been used to determine antibody titers against MG and MS, and genome of these pathogens, respectively. Seventy five percent of farms were seropositive for MG and 94% of farms were seropositive for MS. In addition, the rate of antibody positive flocks against MG were 65.3% (32/49), while the rate of positive flocks against MS were 84.2% (80/95). The geometric mean antibody titers were
against MG and MS, respectively. Interestingly, none of samples was positive for MG genome by PCR, while 94% (farms), 82% (flocks) and 62.6% (broiler breeder) were positive for MS genome by PCR. These findings suggest that the prevalence of MG or MS infection could be higher than expected. Thus, strict prevention program including vaccination and environmental sanitation should be implemented to avoid disease transmission from breeder to broilers as well as transmission among broilers.
Single-tube nested reverse transcription-polymerase chain reaction for simultaneous detection of genotyping of porcine reproductive and respiratory syndrome virus without DNA carryover contamination
Jeong, Pil-Soo ; Park, Su-Jin ; Kim, Eun-Mi ; Park, Ji-Young ; Park, Yu-Ri ; Kang, Dae-Young ; Cha, Hyun-Ouk ; Lee, Kyoung-Ki ; Kim, Seong-Hee ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 107~116
DOI : 10.7853/kjvs.2016.39.2.107
In the study, we developed and evaluated a uracil N-glycosylase (UNG)-supplemented single-tube nested reverse transcription-polymerase chain reaction (UsnRT-PCR) assay that can carried out first-round RT-PCR and second-round nested PCR in a reaction tube without reaction tube opening and can simultaneously detect EU- and NA-PRRSV. The UsnRT-PCR confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PRRSV DNA from previous UsnRT-PCR. Primer specificities were evaluated with RNAs extracted from 8 viral strains and our results revealed that the primers had a high specificity for both genotypes of PRRSV. The sensitivity of the UsnRT-PCR was 0.1
/0.1 mL for EU- or NA-PRRSV, respectively, which is comparable to that of previously reported real time RT-PCR (RRT-PCR). Clinical evaluation on 110 field samples (60 sera and 50 lung tissues) by the UsnRT-PCR and the RRT-PCR showed that detection rates of the UsnRT-PCR was 70% (77/110), and was relatively higher than that of the RRT-PCR (69.1%, 76/110). The percent positive or negative agreement of the UsnRT-PCR compared to RRT-PCR was 96.1% (73/76) or 90.9% (30/33), showing that the test results of both assays may be different for some clinical samples. Therefore, it is recommend that diagnostic laboratory workers use the two diagnostic assays for the correct diagnosis for the relevant samples in the swine disease diagnostic laboratories. In conclusion, the UsnRT-PCR assay can be applied for the rapid, and reliable diagnosis of PRRSV without concerns about preamplified DNA carryover contamination that can occurred in PCR process in the swine disease diagnostic laboratories.
Virucidal efficacy of a fumigant containing orth-phenylphenol against classical swine fever virus and porcine reproductive and respiratory syndrome virus
Cha, Chun-Nam ; Park, Eun-Kee ; Jung, Ji-Youn ; Yoo, Chang-Yeul ; Kim, Suk ; Lee, Hu-Jang ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 117~124
DOI : 10.7853/kjvs.2016.39.2.117
In this study, the virucidal efficacy of a fumigant containing 20% ortho-phenylphenol against classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV) was examined. After each carrier deposited with CSFV and PRRSV suspensions was exposed to the fumigant in a
test room for 15 h, all carriers were neutralized and diluted, and each diluted suspension was inoculated into each proper cell line. After incubation, CSFV and PRRSV viability in each cell line was examined and 50% tissue culture infectious dose
was calculated. In the results, the concentration of viable virus in all of pathogen control-carriers was more than
, and there were no cytotoxicity in all of toxicity control-carriers. In addition, the fumigant inactivated
of both CSFV and PRRSV. These findings will be useful for preventing the spread of CSFV and PRRSV infection.
Prevalence of antibody titers for Coxiella burnetii in cattle in Gwangju area, Korea
Na, Ho-Myung ; Bae, Seong-Yeol ; Koh, Ba-Ra-Da ; Park, Jae-Sung ; Seo, Yun-Jeong ; Jeong, Ha-jin ; Park, Ja-Yoon ; Park, Seong-Do ; Kim, Eun-Sun ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 125~129
DOI : 10.7853/kjvs.2016.39.2.125
The antibody titers against Coxiella burnetii (Q fever) among cattle raised in Gwangju area were analyzed from February to October in 2015. The prevalence of antibodies in collected bulk-tank milk from 7 dairy cattle farms was 57.1% and the seroprevalence of 210 dairy cows randomly selected from those farms was 7.1%. By age, the seroprevalence was 3.3% in less than 4 years of age, 9.0% between 4 and 7 years of age, and 28.6% in more than 8 years of age. On the other hand, the seroprevalence of the Hanwoo cattle was 0.4%. The result suggested that the antibodies against Coxiella burnetii increase as the age of the dairy cows increases and therefore, it is necessary to keep monitoring the prevalence of Q fever in Gwangju area.
Diagnosis on sudden death cases during summer season and isolation of Clostridium novyi
Jeong, Chang-Gi ; Seo, Byoung-Joo ; Kim, Won-Il ;
Korean Journal of Veterinary Service, volume 39, issue 2, 2016, Pages 131~136
DOI : 10.7853/kjvs.2016.39.2.131
Clostridium novyi (C. novyi) is a gram positive, non-capsulated, motile, obligatory anaerobe that produces endospores. Both C. novyi type A and B produce a bacteriophage encoded lethal alpha toxin which belongs to a family of large clostridial cytotoxins. These large clostridial cytotoxins of C. novyi bind to the uncharacterized receptors on host vascular endothelial cells, which leads to the loss of integrity of the vascular endothelium with subsequent edema, refractory hypotension, organ failure, and sudden death. A total of 13 sudden death cases were submitted to Chonbuk National University-Veterinary Diagnostic Center between June and October, 2015. The samples, mainly liver, were collected in sterile vials after necropsy and processed within 12~24 hours for diagnosis, isolation and identification of C. novyi. All of the 4 gram positive samples showed amplification by PCR. Out of 4 positive samples, 3 were detected to be C. novyi type B and 1 was detected as C. novyi type A. Based on the 16S rDNA sequence analysis, 1 case (150564) showed 99% similarity with C. novyi type A while other 3 cases (150388, 150557 and 150775) presented 99% similarity with C. novyi type B. Based on the results, C. novyi was found to be prevalent in Korean pig farms and causes sudden death to finishing pigs or sows during summer season. Thus, C. novyi should be considered for differential diagnosis on sudden death cases during the summer season.