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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Poultry Science
Journal Basic Information
Journal DOI :
The Korean Society of Poultry Science
Editor in Chief :
Volume & Issues
Volume 40, Issue 4 - Dec 2013
Volume 40, Issue 3 - Sep 2013
Volume 40, Issue 2 - Jun 2013
Volume 40, Issue 1 - Mar 2013
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Cryopreservation of Primordial Germ Cells(PGCs) from Korean Native Chicken(Ogye) Embryos using Commercial Cryoprotectants
Kim, Hyun ; Kim, Dong Hun ; Han, Jae Yong ; Do, Yoon Jung ; Kim, Jae Hwan ; Kim, Young Sin ; Seong, Hwan Hoo ; Ko, Yeoung Gyu ; Kim, Sung Woo ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 163~169
DOI : 10.5536/KJPS.2013.40.3.163
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells (PGCs) into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. In the present study, fertilized eggs were incubated for about 5.5 days to obtain embryos at stage 28. The whole embryo was collected from the germinal gonad using a fine glass micro pipette under a microscope. The PGCs were then purified using MACS method. Two commercially available cryoprotectants (A and B) were used to preserve the PGCs, and EG were used as a control. The average recovery rate of PGCs after thawing was 35.5% and 60.5% with the A and B treatments, respectively. There was no significant difference between B treatments and control, which showed an average recovery rate of 52.8%. However, the recovery rate obtained using A cryoprotectant (35.5%) was significantly lower than using treatment control and B. The average viability of the PGCs after thawing were 77.9% and 77.4% for cryoprotectants A and B, respectively, and the control were was 81.6%. There was no statistically significant difference between the two treatments and control. It was concluded that all of the available cryoprotectants examined in this study could be used for preservation of PGCs from embryos. Further experiments to produce germline chimera from PGCs preserved using this techniques are strongly recommended.
Effects of Kinds of Cryoprotectants on the Characteristics of Frozen Fowl Semen
Choi, Jin Seok ; Shin, Dan-Bi ; Ko, Yeoung-Gyu ; Do, Yoon-Jung ; Byun, Mijeong ; Park, Soo-Bong ; Seong, Hwan-Hoo ; Kim, Hyun ; Kong, Il-Keun ; Kim, Sung Woo ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 171~178
DOI : 10.5536/KJPS.2013.40.3.171
The purpose of this study was to evaluate the sperm viability, normal acrosome and mitochondrial activity in the frozen-thawed fowl semen by different cryoprotectants. The experiment was carried out on 10 sexually adult roosters of Ogye. The semen was collected twice a week and pooled semen was diluted 1:1 EK extender containing no cryoprotectant at
. After equilibration for 30 minutes, diluted chicken semen was diluted 1:1 extender containing either 7% dimethylacetamide (DMA), 7% dimethylformamide (DMF) or 7.5% methylacetamide (MA) at final concentration and was put in 0.5 mL plastic straws and frozen for 30 minutes by exposure to liquid nitrogen vapor 4 cm above the surface of liquid nitrogen, followed by plunging into liquid nitrogen. Frozen semen was thawed in water bath at
for 2 minutes. For cytometric analysis, the frozen-thawed semen was diluted with EK extender to a final concentration of 90 million spermatozoa per mL. Sperm membrane integrity was evaluated as SYBR-14 and propidium iodide (PI). Acrosome integrity was assessed with fluorescein isothiocyanate-labeled PSA and PI. The percentage of mitochondrial function was estimated by using Rhodamine123 (R123) and PI. In conclusion, freezing rooster semen by using 7% DMF as cryoprotectant was significantly highest in rates of survival and mitochondrial function while its rate of damage of acrosome was significantly lowest. As a result, DMF is the cryoprotectant that has the lowest influences on sperm membranes and acrosome integrity. Therefore it could be used for freezing method of animal genetic conservation method for poultry diversity.
Effect of Curing Additives on Color Property of Smoked Duck Meat
Kang, Geunho ; Cho, Soohyun ; Seong, Pil-Nam ; Park, Kyoungmi ; Kang, Sun Mun ; Park, Beom-Young ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 179~185
DOI : 10.5536/KJPS.2013.40.3.179
This study was conducted to investigate the effect of curing additives on color property of smoked duck meat. Curing process of samples was performed one of the following treatments: C, non-curing: T1, 2.43% salt: T2, 2.43% salt + 0.49% tripolyphosphate (TPP): T3, 2.43% salt + 0.49% TPP + 0.002% nitrite: T4, 4.76% duck seasoning: and T5, 1.47% salt + 0.24% TPP + 0.2% L-ascorbic acid. Instrumental meat color of both breast and thigh of smoked duck showed that the CIE
value of the T4 was significantly (p<0.05) higher than that of the other treatments, whereas T5 had a significantly (p<0.05) higher CIE
value than the other treatments. In results of nitroso pigment, T5 of smoked duck breast was significantly (p<0.05) higher value compared to other treatments, whereas T3 and T5 of smoked duck thigh had a significantly (p<0.05) higher value than other treatments. Heme pigment contents of control and T5 was significantly (p<0.05) higher value compared to other treatments in smoked duck breast. Meat color of T3 by sensory evaluation showed redder (p<0.05) than other treatments. These results suggested that using L-ascorbic acid is revealed to be pink color without nitrite or pigment when manufacturing of smoked duck meat.
Effects of Dietary Supplementation of Yacon(Polymnia sonchifolia) By-products and Pine Needle Powder on Growth Performance and Meat Quality of Chicken Thigh Meat
Park, Chang-Ill ; Kim, Young-Jik ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 187~195
DOI : 10.5536/KJPS.2013.40.3.187
This study was investigated the effects of dietary supplementation of Yacon by-products and pine needle powder on growth performance, carcass characteristics, pH, TBARS (thiobarbituric acid reactive substance), WHC (water holding capacity), shear force, and meat color of chicken thigh meat. Broiler chicks were fed diets for five weeks containing 0% Yacon by-products and pine needle powder (Control), 0.5% Yacon by-products powder (T1), 1.0% Yacon by-products powder (T2), 0.5% pine needle powder (T3), and 1.0% pine needle powder (T4). There was no significant difference in growth performance among treatments, but mortality was decreased in diets by the supplementation of Yacon by-products and pine needle powder than that of control. The chicken fed control diet had higher cholesterol. LDL-cholesterol. triglyceride compared to those chicken fed Yacon by-products and pine needle powder regardless of concentrations, and HDL-cholesterol of control was lower than treatment. The pH and TBARS were significantly decreased by the supplementation of Yacon by-products and pine needle powder compared to the control (P<0.05), especially, pine needle powder group was significantly more effective in improving freshness compared to other test groups (P<0.05). The WHC and shear force were not significantly different. CIE
value of test groups showed significantly higher value compare to the control, however, no difference in the CIE
values was observed among test groups. In conclusion, a supplementation of Yacon by-products and pine needle powder were effective in decreasing pH, TBARS, total cholesterol and LDL-cholesterol and increasing HDL-cholesterol concentration. Pine needle powder supplementation was most effective (T3 and T4).
Effect of Ethylene Glycol(EG) and Propylene Glycol(PG) on the Viability of Frozen-thawed Primordial Germ Cells(PGCs) on Korean Native Chicken(Ogye) by Vitrification
Kim, Hyun ; Kim, Dong Hun ; Han, Jae Yong ; Choi, Sung Bok ; Ko, Yeoung Gyu ; Do, Yoon Jung ; Seong, Hwan-Hoo ; Kim, Sung Woo ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 197~205
DOI : 10.5536/KJPS.2013.40.3.197
This study established a method for preserving chicken primordial germ cells (PGCs) that enables long-term storage in liquid nitrogen (
) for preservation of the species. The purpose of this study was to compare the effects of Ethylene Glycol (EG) and Propylene Glycol (PG) on viability of cryopreserved PGCs with vitrification in Korean Native Chicken (Ogye), and to fine should be find or to the optimal protocol for PGCs freezing. One of the important components of cryopreservation process is cryopreservation medium that plays a vital role in preventing cellular injury during freeze-thawing. Cryoprotective agents have been known to improve cell viability after freeze-thawing. PGCs obtained from the germinal gonade of 5.5~6 day (stage 28) chick embryos, using the MACS method were suspended in a freezing medium containing a freezing and protecting agents. Gonads were harvested from stage 28 chick embryos and pooled in groups of 10E embryos, contributing gonads to the cell suspension. The gonadal cells, including PGCs, were then frozen in 1 of the following cryoprotectant treatments: 2.5% EG, 5% EG, 10% EG, 2.5% PG, 5% PG, 10% PG, and 0% cryoprotectant as a control. Effects of exposure to vitrification solution and vitrification, with different concentrations of the cryoprotectant solution, were examined. After freezing and thawing, survival rates of the frozen-thawed PGCs from the 0, 2.5, 5, 10 and 15% EG plus FBS treatment were 44.24%, 64.51%, 85.63%, 80.51% and 73.52% (p<0.05), respectively. The viability of PGCs after freeze-thawing was significantly higher for 10% EG plus FBS treatment than for 10% PG + FBS treatment (p<0.05)(85.63% vs 66.81%). Therefore, these systems may contribute in the improvement of cryopreservation for a scarce species in birds preservation. This study established a method for preserving chicken PGC that enables systematic storage and labeling of cryopreserved PGCs in liquid N at a germplasm repository and ease of entry into a database. In the future, the importance for this new technology is that poultry lines can be conserved while work is being conducted on improving the production of germline chimeras.
Comparative Study on the Viability of Frozen-thawed Primordial Germ Cells using Vitrification in Chicken Breed
Kim, Hyun ; Kim, Dong Hun ; Han, Jae Yong ; Choi, Sung Bok ; Ko, Yeoung Gyu ; Do, Yoon Jung ; Seong, Hwan Hoo ; Kim, Sung Woo ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 207~216
DOI : 10.5536/KJPS.2013.40.3.207
This study was conducted to establish the method for preserving PGCs that enables long-term storage in liquid nitrogen for developmental engineering or preservation of species. The purpose of this study is to clarify the effects of freeze-thaw treatment on viability of PGCs in chickens. PGCs were collected separately from a germinal gonad of an early embryo of 5.5~6 day (stage 28) of Isa brown, Korean Oge (KO), White Leghorn and Commercial breeds. PGCs separated from a germinal gonad of an early embryo of 5.5~6 day (stage 28) are suspended in a freezing medium containing a freezing and protecting agents (e.g. dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol). The PGCs were then purified using magnetic activated cell sorting (MACS) method. The viability of PGCs after thawing was
with the 10% EG treatments with no significant difference between the Isa brown and Commercial breeds. The viability of PGCs after freeze- thawing was significantly higher for Isa brown (
) and Commercial breeds (
) than Korean Oge (KO) (
) and White Leghorn (
)(p<0.05) using 10% EG cryoprotectant. This study established a method for pre- serving chicken PGCs that enables systematic storage and labeling of cryopreserved PGCs in liquid (
) at agermplasm repository and ease of entry into a data base. In the future, the importance for this new technology is that poultry lines can be conserved while work is being conducted on improving the production of germline chimeras.
Performance of Middle Laying Period of Large-Type Korean Native Ducks
Heoa, Kang-Nyeong ; Choo, Hyo Jun ; Kim, Chong Dae ; Kim, Sang Ho ; Kim, Hak Kyu ; Lee, Myeong Ji ; Son, Bo Ram ; Kang, Bo Seok ; Choi, Hee Cheol ; Lee, Sang Bae ; Hong, Eui Chul ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 217~222
DOI : 10.5536/KJPS.2013.40.3.217
This work was carried out to investigate the performance of large-type Korean native ducks at middle laying phase (30 to 50 weeks). A total of one hundred and sixty eight laying ducks were divided into A and B strains (6 replications/strain, 14 birds/replication and fed practical diet, for 20 weeks). There was no significant difference in body weight, daily feed intake and average egg weight. With increasing rearing period, mean body weight tended to be decreased and showed the lowest value (2,649 g) at 42 weeks of age (P<0.05). Similar trend was observed for weekly feed intake and average egg weight which decreased with passing weeks and was lowest (190.5 g and 83.8 g, respectively) at the age of 42~46 weeks (P<0.05). Daily egg production of B strain was higher than that of A strain at the age of 46~50 weeks (P<0.05). Weekly egg production of A and B strains commenced to decrease from the age of 42 weeks, however, B strain recovered at 46~50 weeks of age (P<0.05). At the age of 30~38 weeks, the egg production in B strain was higher than A strain (P<0.05), whereas, there was no significant difference between both the strains during 38~50 week. Feed conversion ratio of A strain was higher than that of B strain at 46~50 weeks of age (P<0.05). Weekly feed conversion was highest at the age of 42~46 weeks (P<0.05). In conclusion, the present results provides the basic information of the performance record of large-type Korean native ducks for the middle laying phase.
Marker Assisted Selection-Applications and Evaluation for Commercial Poultry Breeding
Sodhi, Simrinder Singh ; Jeong, Dong Kee ; Sharma, Neelesh ; Lee, Jun Heon ; Kim, Jeong Hyun ; Kim, Sung Hoon ; Kim, Sung Woo ; Oh, Sung Jong ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 223~234
DOI : 10.5536/KJPS.2013.40.3.223
Poultry industry is abounding day by day as it engrosses less cost of investment per bird as compared to large animals. Poultry have the most copious genomic tool box amongst domestic animals for the detection of quantitative trait loci (QTL) and marker assisted selection (MAS). Use of multiple markers and least square techniques for mapping of QTL affecting quality and production traits in poultry is in vogue. Examples of genetic tests that are available to or used in industry programs are documented and classified into causative mutations (direct markers), linked markers in population-wide linkage disequilibrium (LD) with the QTL (LD markers), and linked markers in population wide equilibrium with the QTL (LE markers). Development of genome-wide SNP assays, role of 42 K, 60 K (Illumina) and 600 K (Affymetrix
) SNP chip with next generation sequencing for identification of single nucleotide polymorphism (SNP) has been documented. Hybridization based, PCR based, DNA chip and sequencing based are the major segments of DNA markers which help in conducting of MAS in poultry. Economic index-marker assisted selection (EI-MAS) provides platform for simultaneous selection for production traits while giving due weightage to their marginal economic values by calculating predicted breeding value, using information on DNA markers which are normally associated with relevant QTL. Understanding of linkage equilibrium, linkage dis-equilibrium, relation between the markers and gene of interest are quite important for success of MAS. This kind of selection is the most useful tool in enhancing disease resistance by identifying candidate genes to improve the immune response. The application of marker assisted selection in selection procedures would help in improvement of economic traits in poultry.
Changes of Fatty Acids and Amino Acids Contents of Korean Native Commercial Ducks Meats with Different Raising Periods
Heo, Kang-Nyeong ; Choo, Hyo-Jun ; Kim, Chong-Dae ; Kim, Sang-Ho ; Kim, Hak-Kyu ; Lee, Myeong-Ji ; Son, Bo-Ram ; Choi, Hee-Cheol ; Hong, Eui-Chul ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 235~241
DOI : 10.5536/KJPS.2013.40.3.235
This work was carried to evaluate effect of different raising periods on fatty acids and amino acids properties of Korean native commercial ducks. Korean native ducks (n
Molecular Characterization of an H5N3 Influenza Virus Isolated from Spot-Billed Duck
Lee, Jin Hwa ; Kwon, Hyuk Moo ; Sung, Haan Woo ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 243~252
DOI : 10.5536/KJPS.2013.40.3.243
Among the 16 hemagglutinin (HA) subtypes of avian influenza virus (AIV), only the H5 and H7 subtypes have caused highly pathogenic avian influenza (HPAI) in poultry. However, most H5 or H7 subtype viruses are categorized as low pathogenic avian influenza (LPAI). Some AIVs, including the H5 and H7 HPAI viruses, have shown the ability to infect humans directly. In this study, we describe the biological and molecular characterization of an H5N3 AIV (SBD/KR/KNU SYG06/06) isolated from spot-billed duck (Anas poecilorhyncha) in Korea. A phylogenetic analysis of the eight viral genes showed that the SBD/KR/KNU SYG06/06 isolate belongs to the Eurasian lineage and that the SBD/KR/KNU SYG06/06 isolate was clearly different from HPAI H5N1 strains, including human isolates and the Italian HPAI H5N2 strains. Additionally, no relationship was found between SBD/KR/KNU SYG06/06 and the Korean HPAI H5N1 isolates. The SBD/KR/ KNU SYG06/06 isolate had avian specific receptor binding site residues in the HA protein and the four C-terminal amino acids in the NS1 protein. The HA protein of the SBD/KR/KNU SYG06/06 isolate exhibited the typical LPAI motif at the cleavage site and this virus produced no cytopathic effects in MDCK cells without trypsin. Given these results, we suggest that the H5N3 AIV isolated from the spot-billed duck should be considered an LPAI virus and should have no pathogenic effect in humans.
Effect of Various Forms of Floor System on Performance of Meat-type Duck and Environments of Duck House
Bang, Han-Tae ; Kim, Dong-Woon ; Hwangbo, Jong ; Na, Jae-Cheon ; Kang, Hwan-Ku ; Kim, Min-Ji ; Mushtaq, M.M.H. ; Parvin, R. ; Choi, Hee-Chul ; Lee, Sang-Bae ; Kang, Min ; Kim, Ji-Hyuk ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 253~262
DOI : 10.5536/KJPS.2013.40.3.253
This experiment was conducted to investigate the effects of floor type and heating system on performance, housing environment and health status of ducks reared in three types of duck house (OD : Open floor house-Direct heating system, OF : Open floor house-Floor heating system and LD : Loft type house-Direct heating system). In OF treatment, PVC pipes were installed for heating under concrete floor and covered with litter. In LD treatment, plastic mesh was installed 50 cm above the floor so that duck`s droppings can pass through it. Each treatment had four replicates of 25 birds (Cherry Valley duck breed) per pen. There were no significant differences in weight gain and feed intake of ducks for 6 weeks among all treatments. However, feed conversion ratio in LD was significantly higher (p<0.05) than that in OF. No differences were found in carcass charac- teristics, with the exception of abdominal fat weight where OF were higher than the others. Concentrations of
gas in OD were higher than those of OF and LD at 3, 4 and 5 weeks. Moisture content in litter of OF was lower than that of OD. In contrast, the amount of dust in the air was higher in OF than in OD. The amount of fuel used for 6 weeks in LD was lower about 21% than that in OD. Some of unusual symptoms were observed in open floor house and loft type house, such as lying, spraddle legged, twisted ankle and legs, wounded sole, or etc. No components of leukocyte and erythrocyte of blood were significantly different among all treatments. The results of this experiment showed that OF and LD systems had no positive effects on performance of meat type commercial duck. However, there were some positive effects of certain house type for the improvement of environmental condition in duck house for hygienic production. In the future, more research on the effect of various facilities and systems for duck house is needed.
Influence of Early- and Late-feathering Phenotype on Productive Performance in the Feather-sexing Strains of Korean Native Chicken
Sohn, Sea Hwan ; Kim, Na Young ; Park, Dhan Bee ; Song, Hae Ran ; Cho, Eun Jung ; Choi, Seong Bok ; Heo, Kang Nyeong ; Choi, Hee Cheol ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 263~270
DOI : 10.5536/KJPS.2013.40.3.263
The vent sexing and the auto-sexing by using sex-linked traits are general sexing methods of day-old chicks. Currently, the feather sexing which is based on the differences in the feather characteristics at hatching is the representative sexing method of chicken, because the late-feathering is sex-linked trait. The feather sexing can be used if the breed has dominant feathering gene (K) in maternal and recessive gene (
) in paternal. Therefore it is necessary to identify the association of feathering genes and quantitative traits in chickens. In this study, we investigated the influence of the rate of feathering on productive traits in Korean Native Chicken. In results, there was no significant difference between early-feathering chickens and late-feathering chickens in reproductive performance such as fertility and hatchability. Livability, body weights, egg production, egg weight and egg quality also did not significantly differ between early- and late-feathering chickens. Age at first egg was the only trait of those tested in which significant difference was observed. The early-feathering chickens laid eggs 3 days earlier than late-feathering chicken. As a result, there is no influence of feathering phenotypes on productive performance in Korean Native Chickens. Consequentially, establishing the feather sexing strain is available using the Korean Native Chicken breed without considering of the effect of feathering genes on productive traits.
Effect of Dietary Chlorella Supplementation on Growth Performance, Immune Response, and Intestinal Micro Flora Concentration of Broiler Chickens
Kang, Hwan Ku ; Choi, Hee Chul ; Kim, Dong Woon ; Hwangbo, Jong ; Na, Jae Cheon ; Bang, Han Tae ; Kim, Dong Wook ; Kim, Min Ji ; Mushtaq, M.M.H. ; Parvin, Rana ; Kim, Ji Hyuk ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 271~276
DOI : 10.5536/KJPS.2013.40.3.271
A study was conducted to investigate the effect of dietary supplementation of feedstuff of Chlorella (Chlorella vulgaris) to replace of antibiotic in the diets of broiler chickens. A total of 720 1-d-old straight run broiler chicks (Ross
Ross) was randomly assigned into six treatments with four replicate pens (30 birds/replicate pen) for 5-wk. A corn-soy bean meal basal diet was formulated, the treatment groups were negative group (NC, antibiotic-free diet) and 0.1% virginiamycin in as antibiotic growth promoters (PC), 1.0% fresh liquid Chlorella (T1), 1.0% dried Chlorella powder (T2), 1.0% commercial Chlorella product and 1.0% (T3) and commercial Chlorella product 0.5% (T4) were added to the basal diet to form six dietary treatments. No significant differences were found among the treatments for feed intake and feed conversion of broiler chickens during the whole experimental period, but the BW gain was significantly higher (P<0.05) in commercial Chlorella product supplemental groups than the control group (NC and PC groups). Dietary supplementation of Chlorella significantly (P<0.05) increased the plasma IgA, IgM and IgG concentration of chicks compared to NC and PC groups. Supplemental AGPs and commercial chlorella product did not affect the E. coli and Salmonella concentration in the intestinal microflora of broiler chicks; however, the population of Lactobacillus was significantly increased (P<0.05) when birds were fed commercial Chlorella product groups. It is concluded that commercial Chlorella product supplementation could be used as an alternative of antibiotics to promote growth and immune response by increasing the production of lactic acid bacteria in the intestinal microflora of broiler chickens.
Effects of Bacillus subtilis on Growth Performance and Resistance to Salmonella Infection in Broiler Chickens
Yoo, Jae Hong ;
Korean Journal of Poultry Science, volume 40, issue 3, 2013, Pages 277~281
DOI : 10.5536/KJPS.2013.40.3.277
The experiment was undertaken to see the effects of Bacillus sp. on the growth performance and disease resistance to Salmonella sp. infections. The use of probiotic microbes in poultry is commonly practiced. In this study, Bacillus subtilis was tested using a total of 120 chicks of age of 1 day after hatching. The growth traits examined were body weight gain and feed conversion rate. And also, the Salmonella resistance of Bacillus subtilis was tested after the chicks were orally administered with Salmonella pullorum by gavage force injections. The result showed that Bacillus subtilis yielded a high feed efficiency, consequently increased growth rate. For the effect of Bacillus subtilis on Salmonella infection, Bacillus subtilis significantly improved the resistance to Salmonella pullorum infection. Various clinical symptoms of Salmonella infection were highly decreased by addition of Bacillus sp.