Highly phosphorylated nucleotides were investigated to assure whether the eucaryotic Aspergillus niger produce these substances or not during the differentiation. Investigation was extended to see how organic phosphate interacts with inorganic polyphosphate during development, and high molecular weight RNA-polyphosphate complex was detected in 2.6% polyacrylamide gel by electrophoresis. Guanosine tetraphosphate was found in vesicle and phialide forming mycelia and spore forming body by PEI cellulose TLC. It is revealed that guanosine tetraphosphate is a common substance for spore formation in eucaryotic microorganisms as well as in procaryotic. Especially, prior to sporulation, protein bound RNA and protein bound phosphate may occur as a result of reorganization of cellular materials. The evidence was obtained by the fact of differential increase of optical density ratio between the samples from different developmental stages of this fungus. In 2.6% polyacrylamide gel which was run to electrophoresis, high molecular weight RNA (mostly rRNA) was found to couple and to make RNA-polyphosphate complex. The complex was examined with enzymes and radioactive isotope of . (enzymic test was not reported here.) RNA-polyphosphate complex might be another sort of highly phosphorylated nucleotide or rRNA beside guanosine-tetraphosphate.

The (70%) treated crude enzymes from the culture filtrates of the strain of Pyricularia oryzae which was grown on 2% CMC (carboxymethyl cellulose) for 8 days at were chromatographied on Sephadex G-150 and DEAE-Sephadex A-25 columns. From the chromatography, three fractions of CMCase was examined using Na-CMC as substrate. The enzyme activity was optimal at pH 6.0 and , stable up to . The values of Km and Vmax of the enzyme were and 5.9m moles/hour, respectively. The molecular weight determined by Sephadex G-150 column chromatography was around 80,000. Approximately sevenfold purified enzyme gave a single protein band on the polyacrylamide gel electrophoresis.

As a primary study for cell growth and alcohol production of a cider yeast, Saccharomyces sp. R-11, cultural and nutritional characteristics of the strain were investigated. The results obtained were as follows: The optimum culture medium for this strain was a synthetic medium, Henneberg B, and sucrose was the best carbon source for yeast growth and alcohol production. Optimum sugar concentrations for yeast growth and alcohol production were 15% and 25%, respectively. Optimum pH and temperature of the basal medium for growth of this strain were 4.5 and respectively. The yeast growth was enhanced by the addition of 100 ppm of , but significantly inhibited by the addition of 100 ppm of . Lower temperature and maintenance of optimum pH for yeast growth increased the final alcohol concentration. Under optimum condition for cell growth at stationary culture, generation time and specific growth rate of the strain were 7.5 hr and 0.092 , respectively. At 8% initial alcohol concentration, yeast growth was inhibited about 50% and this strain could not be grown at more than 12% initial alcohol. The strain could be grown at less than 125ppm without alcohol addition, and at less than 75 ppm with 8% initial alcohol. The higher sulfur dioxide concentration of a medium, the longer lag phase in yeast growth was observed. This strain could induced alcoholic fermentation at less than 10% initial alcohol concentration with 0 and 25 ppm , at less than 8% initial alcohol with 50 and 75 ppm , and at less than 6% initial alcohol with 100 and 125 ppm .

In order to investigate flight number of ascospores of the wheat scab fungus as a primary inoculum, the experiment was undertaken at three localities including Suweon during the two years 1979 to 1980. Ascospore fllight of the fungus was begun at the first part of April, and amounts of flight number of ascospores were varied by different localities. It showed a tendency that large number of ascospore fllight was proportionally resulted by higher amounts of rainfall. As a result, a high positive correlation was obtained between flight number of ascospores of the fungus and amounts of rainfall.

The seeds of 20 barley cultivars were tested for aflatoxin contamination and susceptibility to infection by an aflatoxin-producing mold. When the samples were tested as they arrived, no aflatoxin was detected on any of them. When their moisture was raised to 25% and they were kept as for 2 weeks, all expect 2 cultivars showed aflatoxin contamination. Aflatoxins and were not detected in this incubation period. After wetting (25% moisture) the samples, inoculating them with Aspergillus parasiticus conidia and storing them at for 2 weeks, all cultivars were found heavily contaminated with aflatoxin, those with seedcoats more so than those without seedcoats.

Soybean Whetzelinia rot caused by Whetzelinia sclerotiorum was observed in Jinju area. The diseased soybean plants showed withering and sudden collapse under field conditions. Diseased parts exhibited numerous black, irregularly-shaped scleratia embedded in dense white cottonly mycelium on tissue and in the pith of diseased stems. A sclerotium in the moist sand produced several apothecia under laboratary condition. The primary inoculum was supposed to originate from overwintered sclerotia of soil and soybean debris.