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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 12, Issue 4 - Dec 1984
Volume 12, Issue 3 - Oct 1984
Volume 12, Issue 2 - Jul 1984
Volume 12, Issue 1 - Apr 1984
Selecting the target year
Studies on the Benomyl Resistance of Oyster Mushroom (Pleurotus spp.)
Yoo, Sung-Joon ; Shin, Gwan-Chull ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 1~8
The mycelial growth of some mushrooms was inhibited by benomyl treatment. The
of benomyl to that of Pleurotus spp., Agaricus bisporus and Flammulina velutipes was 25ppm, 50ppm and 200ppm, respectively, which indicates the former was the most sensitive to the fungicide. The mycelial growth of mushrooms growing on artificial media amended by benomyl was increased when they were cultured successively 5 times and 10 times on the media. The increasing rate of that of each mushroom was the highest at the concentration of
of benomyl. The mycelial growth of P. ostreatus was increased progressively as the number of successive culturing increased, while that of P. florida and A. bisporus was increased until they were cultured successively up to 5 times and 7 times, respectively, but they were decreased after that. Mutant sectors of mycelia were induced by successive treatment of benomyl. Mutant sectors of P. ostreatus appeared earlier than those of P. florida and all of them were induced earlier on the media of low contration of benomyl than on that of high concentration. The mycelia of mutant sectors induced by benomyl treatment grow faster than those of mother colony treated with benomyl successively, but there was no difference in resistance against the fungicide between them. The increase of mycelial growth of the mushrooms culturing successively on media containing benomyl indicated that they might obtain the resistance against benomyl.
Some Factors Affecting the Protoplast Release from Pleurotus ostreatus
Byun, Myung-Ok ; Go, Seung-Joo ; Park, Yong-Hwan ; Shin, Gwan-Chull ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 9~14
Some factors affecting the protoplast release from mycelia of Plurotus ostreatus using commercial lytic enzymes were investigated. The highest yields of the protoplast were obtained from four days old mycelia grown in mushroom complete medium. The solution of 0.8M
, or KCI showed good results as the osmotic stabilizer for releasing the protoplast. Novozym 234 was the most effective among commercials tested. The concentration of the enzyme and pH of the enzyme solution were optimal at 15mg/ml and
for the protoplast release, respectively. Mycelial digestion was optimal at about
and was better in the reciprocal shaking bath (75 oscillations/min) than the stationary culture.
Effect of Temperature, pH, Carbon and Nitrogen Nutritions on Mycelial Growth of Pleurotus sajor-caju (Fr.) Sing. and Pleurotus ostreatus (Fr.)
Go, Seung-Joo ; You, Chang-Hyun ; Park, Yong-Hwan ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 15~19
Some factors affecting mycelial growth of Pleurotus sajor-caju and P. ostreatus. were investigated. The optimal temperature and pH of the medium for mycelial growth of both the species were
, and 5 to 6, respectively. The effect of carbon and nitrogen sources on mycelial growth of the species was studied using modified Waksman's special medium as the basal medium. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of P. sajor-caju, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth of P. ostreatus. Generally the organic nitrogen compounds showed good mycelial growth of both the species. The mycelial growth of the species were favored by the ammonium nitrogen more than by the nitrate nitrogen source. Asparagine was superior to the other kinds of amino acid. Six percentage of glucose and 0.6% ammonium tartarate was optimum for mycelial growth of P. sajor-caju whereas the optimum concentration for the P. ostreatut was 4% glucose and 0.4% ammonium tatarate.
Application of Glucose Oxidase for the Rapid Screening of Glucoamylase-producing Fungus
Moon, In-Kyung ; Lee, Hyung-Hoan ; Kim, Jong-Hyup ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 21~26
Aspergillus awamori which produces glucoamylase was cultivated in the starch-Czapek-Dox's medium in which sucrose was depleted. A rapid method for identification and assay of glucoamylase produced by the A. awamori in the culture was established by the use of the glucose oxidase. The levels of glucose derived from the breakdown of the starch medium were assayed by using glucose oxidase, which was proved to be effective in the screening of glucoamylase-producing fungi in terms of rapid and simple determination. After the cellulose acetate electrophoresis of the precipita ted culture broth, the glucoamylase band in the gel was contacted with 2% starch solution with glucose oxidase, and then color reaction was occurred. Also this method could be effective to identify rapidly the fungal glucoamylase.
Fungi Associated with Soybean Seed, their Pathogenicity and Seed Treatment
Lee, Du-Hyung ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 27~33
Alternaria tenuis, Arthrobotrytis sp., Aspergillus spp., Cephalosporium sp., Cladosporium sp., Cylindrocarpon sp., Fusarium equiseti, F. moniliforme, F. semitectum, F. solani, Penicillium spp., Rhizopus sp. were saprophytic fungi and Cercospora kikuchii, Colletotrichum truncatum, Diaporthe phaseolorum var. sojae and Fusarium oxysporum were pathogenic fungi detected from 14 seed samples of soybean. Initial symptoms caused by C. kikuchii, C. truncatum, D. phaseolorum sojae and F. oxysporum on seedlings from naturally infected seed by the test tube agar method have been described and discussed. Soybean seeds infected with C. truncatum, D. phaseolorum sojae and F. oxysporum reduced germination of seeds and have influenced on the growth of soybean seedling caused by C. kikuchii. Surface-sterilized soybean seedlings became diseased in the test tube agar artificially inoculated with C. kikuchii, C. truncatum and D. phaseolorum sojae isolated from naturally infected soybean seeds. F. oxysporum showed very weak pathogenicity. Seed disinfectants of Benlate-T, Homai, Tecto and Sisthane have effective to C. kikuchii, C. truncatum, D. phaseolorum sojae and F. oxysporum. Arasan, Captan, Busan-30 and Mercron were inferior to C. kikuchii but effective against others. Seed disinfectants treated in this experiment have increased seed germination campared with non-treatment.
Studies on Antitumor Components of Cultured Basidiomycetes - Purification and Chemical Analysis of Antineoplastic Constituents of Cultured Mycelia of Laccaria laccata -
Kim, Yoo-Jin ; Lee, Chong-Ock ; Shim, Mi-Ja ; Kim, Sung-Won ; Choi, Eung-Chil ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 12, issue 1, 1984, Pages 35~43
To produce and characterize antineoplastic constituents in the submerged culturedmycelia of Laccaria laccata, the mycelia were extracted with distilled water. Purification of the extract was carried out by acetone precipitation, by ion exchange chromatography using DEAESephadex A-50, CM-Sephadex C-25 resins, and by gel filtration chromatography on Sephadex G-200. Each fraction obtained during the purification was examined for antineoplastic activity against sarcoma 180 in ICR mice. As the purification proceeded, the antineoplastic activity was markedly increased. The highly purified Fraction E showed 75% tumor inhibition ratio at a dose of 10mg/kg/day and contained 81% polysaccharide and 4% protein. The antitumor component of Fraction E stimulated an accumulation of peritoneal exudate cells including peritoneal macrophages, and is named laccaran.