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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 13, Issue 4 - Dec 1985
Volume 13, Issue 3 - Sep 1985
Volume 13, Issue 2 - Jun 1985
Volume 13, Issue 1 - Mar 1985
Selecting the target year
Studies on the Distribution of the Mononucleotides in Aspergillus niger during Differentiation
Kim, Boon-Lae ; Kim, Jong-Hyup ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 65~74
Aspergillus niger IMI 41873 was cultured by the method of synchronous and submerged culture. Its sporulation occurred in the culture. Ribonucleic acids were extracted at each stage of life cycle. These RNAs were digested, separated and determined by P.E.I. cellulose TLC and HPLC methods. The levels of ribonucleic acids in sporulating mycelia were higher than those of conidiophore and phialide forming mycelia. Inosine 5-monophosphate and adenosine 5-monophosphate derivatives were found in HPLC separations. The levels of inosine 5-monophosphate and adenosine 5-monophosphate derivatives per ribonucleic acid were constant through differentiation. After the standard purine necleosides and boiling water extracts from A. bisporus, F. velutipes and L. edodes were added into the culture, their effects on sporulation were examined. Sporulation was greatly enhanced in each adding experiment.
Isolation of Auxotrophic Mutants from Protoplasts of Pleurotus ostreatus and Pleurotus florida
Yoo, Young-Bok ; Peberdy, John F. ; Park, Yong-Hwan ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 75~78
Mutagenesis of protoplast could serve a great potential tool for improvement of strains and genetics in higher fungi. For the isolation of auxotrophic mutants from protoplasts of Pleurotus ostreatus and Pleurotus florida, viability levels of ultraviolet lights were determined. Seven auxotrophs were obtained from protoplasts irradiating UV to give
survival. The mutants showed a single requirement for each of Arg, Ribo-l, Ribo-2 or Phen for growth. Some of them showed two or three kinds of requirements, Gly Ser, Ade Hypo or Ala Om Tryp for growth.
Studies on Protoplast Regeneration and Reversion of Pleurotus ostreatus and Pleurotus florida
Yoo, Young-Bok ; Peberdy, John F. ; Cha, Dong-Yeul ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 79~82
The experiment of protoplast regeneration and reversion were undertaken to provide a basic techniques for protoplast manipulation. Protoplasts of Pleurotus florida and Pleurotus ostreatus were reverted to normal hyphal growth and the reversion frequency of both fungal protoplasts were
. Reversion medium stabilized with 0.6 M potassium chloride and sucrose was better than the other stabilized one. The protoplast reversion frequency was increased when various amino acid and vitamin compounds were added to the hypertonic mushroom complete agar medium.
Steroid Modification with Aspergillus phoenicis -Effects of Reaction Temperature and Sonication-
Kim, Mal-Nam ; Lee, Young-Jong ; Lee, Hyung-Hoan ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 83~87
The temperature dependency and the stability of enzyme systems for
of progesterone were investigated using Aspergillus phoenicis. Though A. phoenicis conserves high enzyme activities for lactose hydrolysis even at high temperatures, the bioconversion reaction of progesterone by this strain was found to be very temperature sensitive. The compositions of reaction mediums of inside and outside of cells were analyzed using sonication technique. At early stage of reaction, the concentration of
of cell inside was higher than that of outside. But as the reaction proceeded further, the
existing inside of cells being converted into another products, its concentration was lower within the cells that in the bulk medium. Even in the reaction mediums containing organic solvents, A. phoenicis was founded to be able to metabolite, so that
can be produced continuously from fixed bed reactions packed with immobilized A phoenicis in vivo.
Studies on Antibiotic Producers of Korean Soil Microbes (IV) -Isolation and Antibiotic Activity of Streptomyces Strain DMC-42-
Kim, Hwa-Ki ; Kim, Jung-Woo ; Kim, Ha-Won ; Choi, Eung-Chil ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 89~97
To find antibacterial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antibacterial activity against several standard microorganisms. An isolate among them had antibacterial activities against gram-positive and gram-negative bacteria. The examination of its morphological, biochemical, cultural and physiological characteristics according to the International Streptomyces Project methods showed that it belongs to the genus Streptomyces. This strain appears to be a novel strain when it was compared with those species of the genus which have been so far reported. The antibiotic metabolite was produced in the submerged culture of the strain. This metabolite was extracted from the culture filtrate and purified by ion-exchange column chromatography. Physico-chemical properties of the antibacterial metabolite were characterized.
Rice Seed Infection of Fusarium spp. at Paddy Field and Selection of Seed Disinfectants
Sung, Jae-Mo ; Lee, Eun-Jong ; Yang, Sung-Suk ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 99~103
F. roseum "Graminearum" and F. moniliforme were isolated from hulls of seeds collected from fields. F. roseum "Graminearum" was isolated from brown rice of all the varieties tested. F. moniliforme was isolated from Nagdongbyeo, Seomjinbyeo, Sinsunchalbyeo, Milyang 23, Baegyangbyeo and Samnambyeo. F. moniliforme was isolated from the most of the seeds damaged by sparrow and it formed sporodochia of this fungus. Five species including F. moniliforme were isolated from plant hopper collected from paddy fields in September. To select seed a disinfectant, twelve fungicides were tested and P242 was one of the best effective fungicidetested. After seed treatment with the fungicide, F. moniliforme was not isolated from hulls but was frequently isolated from brown rices.
Influences of Antibiotic Components Produced by Trichoderma spp. to Oyster Mushroom
Kim, Myoung-Kon ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 105~109
Isolates of the different species groups of Trichoderma from the mushroom culture beds were identified according to Rifai's classification and influence of antibiotics produced by them against the oyster mushroom was examined. Trichoderma islolates were identified as Trichoderma hamatum, Trichoderma viride and Trichoderma koningii. Among the Trichoderma isolates, fungistatic action of Trichoderma viride was found to be most remarkable. Pleurotus ostreatus and Pleurotus sajor-caju were the most susceptable of the edible mushrooms tested, followed by Lentinus edodes, Flammulina velutipes and Auricularia auricula. A needle-shaped crystal gained from the chloroform extract of the culture filtrate of Trichoderma viride repressed distinctively the mycelial growth of the oyster mushroom. The grade of repression of the crystal at 500ppm and 1/10 aequous solution of the chloroform extract against the oyster mushroom, seemed equal to that of cycloheximide at
Production and Enzymatic Properties of Laccase from Flammulina velutipes
Lee, Jae-Sung ; Suh, Dal-Sun ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 111~114
The production of lac case by the funguson various media was studied. The characteristics of the enzyme were also studied regarding to the optimum pH, stability, Km value, and inactivation. The maximum activity of laccase reached the 40 days of incubation and the barley straw extract appeared to be a strong inducer for laccase. The enzyme showed stability at wide range of pH with optimum pH of 6.6. Temperature stability of the enzyme was high. Laccase was not inactivated by the organic solvents used for the precipitation. The enzyme, however, was completely inactivated by trichloroacetic acid and sodium azide.
Air-borne Fungi in the Air of Seoul
Min, Kyung-Hee ; Lee, Yung-Ja ;
The Korean Journal of Mycology, volume 13, issue 2, 1985, Pages 115~121
An aerial survey for fungal flora in Seoul has been carried out by Petri plate exposure method for a period of one year. A total of 2227 fungal spores appeared in the plates. Of these, Cladosporium was the commonest fungus representing 43.5% of the total spore count followed by Penicillium (15.8%). Alternaria (10.5%), and Aspergillus (3.8%). There were seasonal variations in the prevalence of fungal spores. The occurrence of these fungi was greatly affected by climatic conditions. However, Alternaria appeared to prefer warmer weather. A comparison of fungal population from the outdoor and indoor air was also investigated to support the view that Alternaria is abundant population from the outdoor air and that Cladosporium from the indoor air depending on their availability in the atmosphere.