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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 13, Issue 4 - Dec 1985
Volume 13, Issue 3 - Sep 1985
Volume 13, Issue 2 - Jun 1985
Volume 13, Issue 1 - Mar 1985
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Studies on Constituents of the Higher Fungi of Korea(XLVII) -Antitumor Constituents of the Cultured Mycelia of Agaricus campestris-
Park, Hee-Ju ; Kim, Ha-Won ; Woo, Myoung-Sik ; Shim, Mi-Ja ; Park, Wan-Hee ; Choi, Eung-Chil ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 131~139
To find antitumor constituents in the shake cultured mycelia of Agaricus campestris, the mycelia were extracted with hot water. Purification of the extract was carried out by ethanol precipitation and by ion exchange chromatography using DEAE-Sephadex A-50. Each fraction obtained during the purification procedure was examined for antitumor activity against sarcoma 180 in ICR mice. The antitumor fraction C was isolated. It showed 56.1% inhibition ratio at a dose of 20 mg/kg/day and consisted of a polysaccharide moiety (45%) and a protein moiety (18%). The polysaccharide was analyzed by G.L.C. and found to contain mannose (42.0%), glucose (25.5%), xylose (16.6%), fucose and galactose. The protein moiety was composed of 17 amino acids. The antitumor fraction A showed immunopotentiating activity by accumulating peritoneal macrophages and by increasing plaque-forming cells in mice.
Cytochemical Observation of Volutin Granules and Activities of Tripolyphosphatase and Polyphosphatase in Saccharomyces uvarum
Lee, Ki-Sung ; Choi, Yong-Keel ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 141~148
To investigate cellular regulation of phosphate metabolism between catabolically repressed and derepressed states in Saccharomyces uvarum, the activities of polyphosphatases, the analysis of polyphosphate and cytochemical observation of volutin granules were examined according to the culture phase and under various phosphate concentrations. As the results, tripolyphosphatase activity was increased more than six-fold during catabolic repression as compared with those of catabolic derepression and the polyphosphatase activity increased at the time of maximal accumulation of acid insoluble polyphosphate 'B'. Of the low molecular weight polyphosphates, tripolyphosphate was mainly detected by thin layer chromatography. When the synthesis of volutin granules in derepressed cells was observed cytochemically, acid insoluble polyphosphate localizing at the cell wall was primarily synthesized and then transferred into the cytoplasm, nucleus and/or vacuole.
Effects of Red Ginseng Saponins on Growth of and Aflatoxin Production by Aspergillus flavus
Park, Hyo-Jin ; Bahk, Jae-Rim ; Song, Dong-Sook ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 149~156
The study was carried out to find effects of the saponins that were extracted from red ginseng on the growth of, aflatoxins production by, and protein patterns of Aspergillus flavus NRRL 3357. A. flavus with
conidia as grown at
for seven days on the enriched medium. Mycelial growth and pH changes of medium which cultured the mold, were similar to those of the control group. However, aflatoxin which produced by the mold was less than that of the control in all concentration of the saponin. To be more specific, 0.3 % of the saponin inhibited production of aflatoxin
to the extent of 31.6 and 21% of the control. The protein peaks of A. flavus at the fourth day of the culture were shown high intensity near the level of 14,300 daltons. However, the mold which cultured in the medium containing the saponin showed low intensity of protein than that of the control group on all molecular weight.
Conversion of Fermented Feed by Basidiomycetes
Hong, Jae-Sik ; Kim, Joong-Man ; Jeong, Jin-Chul ; Lee, Tae-Kyu ; Kim, Dong-Han ; Kim, Myung-Kon ; Lee, Keug-Ro ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 157~168
To investigate nutritive values of a feed fermented with basidiomycetes, among the isolated strains, Lyophyllum decastes (Fr.) Sing. was found with the greatest enzyme productivity and rapid mycelial growth in rice straw medium. Optimum temperature, pH and moisture content for mycelial growth and enzyme production of the strain were
, respectively. Fifteen days of culture were required for the highest enzyme productivity. Among the sub-materials added,
of rice bran and
of defatted perilla seeds were effective for the enzyme production, but caused a reduced mycelial growth. The greatest effect of an addition of inorganic salts was obtained with
. When 40 mesh or smaller rice straw and steam treatment at
were used, the mycelial growth decreased, whereas the enzyme production increased. The mycelial growth and enzyme production increased when
was used as the alkali treatment, but decreased with increasing concentration of NaOH. As the fermentation proceeded, the amounts of ash, reducing sugar and total nitrogen increased, but cellulose, lignin and pentosan decreased. When the rice straw was treated with alkali, the amounts of ash, total nitrogen and lignin decreased, but reducing sugar and cellulose increased. At higher NaOH concentration, the variation become greater. The in vitro dry matter digestibility of the products increased from 55.03 % at the beginning of the fermentation to 62.72 % at 45 days after fermentation. The most effective alkali treatment on the digestibility of rice straw was KOH followed by NaOH. However, the digestibility increased with increasing concentration of NaOH. The digestibility of pretreated with alkali increased after fermentation as well.
Protoplast Formation, Regeneration and Reversion in Pleurotus ostreatus and P. sajor-caju
Go, Seung-Joo ; Shin, Gwan-Chull ; Yoo, Young-Bok ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 169~177
The studies were carried out to obtain the basic data for maximizing the protoplast yields from the mycelia of P. ostreatus and P. sajor-caju. Some factors affecting the regeneration of the protoplast of both species and the productivity of their reversion were also examined. The maximum yields of protoplasts were obtained from four days cultured mycelia of both species on cellophan membrane placed on the surface of PSA or MCM media in a petri dish. The optimal concentration of lytic enzyme Novozym 234 for protoplast releasing was 5 mg per ml of 0.5 M phosphate buffer solution with 0.6 M sucrose or 0.6 M
at pH 6.0. The greatest number of protoplasts was released 3 hours after incubation of the mycelia of P. ostreatus and after 4 hours for the P. sajor-caju in the lytic enzyme solution. Among the osmotic stabilizer solutions tested 0.6 M sucrose and 0.6 M KCl showed the best regeneration rates of the protoplasts of both species. When 0.75 % agar solution was over-layed on the regeneration media immediately after inoculation of the protoplast the regeneration rates were greatly enhanced. The ampicillin added to the agar solution prevented bacteria from infection. The reverted isolates produced the sporophores and basidial spores just like their parents without any mutations when they were cultivated in a broad mouth bottle with sawdust substrates.
Identification of Cylindrocarpon destructans Associated with Root Rot Disease of Strawberry
Sung, Jae-Mo ; Song, Yoong-Nam ; Yang, Sung-Suk ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 179~183
Cylindrocarpon spp. were isolated from the soil where strawberry was grown in Suweon by soil plate method: colonies reaching 10 mm diam. in seven days at about
; sporodochia with cream to beige to conidial slime commonly produced; conidiophore repeatedly branched and bearing subulate phialides; macroconidia cylindrical in the center part, straight or slightly curved and mostly
; chlamydospore abundantly produced, intercalary or terminal on mycelium, singly or in chains and smooth or warted. The hypha and spore were easily fused each other on water agar. This fungus was pathogenic strawberry as a result of inoculation test. The symptom showed dwarf and yellowing at top and rotted roots under the ground. The fungus was identified as Cylindrocarpon destructans Scholten from the shape of conidiophores and conidia, mycelial growth and pathogenicity test.
Isolation of Auxotrophic Mutants from Basidiospores of Lentinus edodes
Yoo, Young-Bok ; You, Chang-Hyun ; Park, Yong-Hwan ;
The Korean Journal of Mycology, volume 13, issue 3, 1985, Pages 185~189
This experiment was undertaken to investigate considerable economy of labor for selection of auxotrophs from strains of Lentinus edodes. Various types of auxotrophs of this strain were isolated after treatment of basidiospores with ultraviolet light, and the highest proportion of putative mutants was also obtained from isolates irradiated to give 1.30 % survival.