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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 17, Issue 4 - Dec 1989
Volume 17, Issue 3 - Sep 1989
Volume 17, Issue 2 - Jun 1989
Volume 17, Issue 1 - Mar 1989
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Studies on Analyzing Meteorological Elements Related with Yield of Tricholoma matsutake (S. Ito et Imai) Singer.
Kang, An-Seok ; Cha, Dong-Yeol ; Kim, Yang-Sup ; Park, Yong-Hwan ; You, Chang-Hyun ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 51~56
This study was carried out to obtain basic information elucidating relationship meteorological elements and yield of Tricholoma matsutake. The yields of pine mushroom in seven areas including Yangyang-gun more than 30,000 kg. Pine mushrooms were generally distributed in the red pine (Pinus densiflora) forests of East-Coast Region, Sobaek and Taebaek-Mountainous Regions, Yeongnam-Central Region and North-Central Region of Korea. Yields of pine mushroom were significant correlation with precipitation during April-October in last years, average minima temperature during July-September, rainy days during Aug. 21-Sep. 10 and precipitation during Sep. 11-30, etc. Air and soil temperature during pine mushroom yield period were 15-25 and
, respectively. The first collection date of pine mushroom could be predicted by measuring the soil temperature at 5 cm depth.
Synergistic Effect of Substrates on the Biosynthesis of Cellulase and Xylanase Complexes from Aspergillus nidulans
Lee, Jeong-Ae ; Maeng, Jin-Soo ; Maeng, Pil-Jae ; Rhee, Young-Ha ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 57~65
The effect of various cellulosic and hemicellulosic substrates on the induction of cellulase and xylanase complexes in Aapergillus nidulans was investigated. The most efficient substrates for the induction of cellulase and xylanase complexes were carboxymethylcellulose for endoglucanase, cellobiose for
, and xylan for endoxylanase and
, respectively. However, the mixtures of these substrates, especially CMC-xylan and CMC-xylan-laminarin mixture, were much more effective not only for the enhancement of the biosynthesis of all the cellulase and xylanase complexes but also for the balanced production of these enzyme components than individual substrate. The polyacrylamide gel electrophoresis followed by activity staining showed the variation in the patterns and relative intensity of
, endoglucanase and endoxylanase components in individual enzyme preparations from A. nidulans cultures grown on different substrates. These results suggest that the biosynthesis is of cellulase and xylanase systems in A. nidulans is regulated in coordination at the level of induction.
Analysis of Culture Filtrate Antigens of Aspergillus fumigatus Strains and of Antibody Responce in Patients with Aspergillosis by Immunoblotting
Kim, Sang-Jae ; Kim, Sin-Ok ; Hong, Young-Pyo ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 66~75
Heterogeneity in antigenic composition of Aspergillus fumigatus isolates from clinical specimens and in antibody response of patients infected with this fungus was investigated by immunoblotting. A considerable quantitative and qualitative difference was found in composition of the culture filtrate antigens derived from a reference strain (ATCC 13073) and 8 clinical isolates of A. fumigatus on SDS-PAGE and immunoblots. The crude CF antigen of a strain AFG7 was selected to identify the serologically reactive and specific components by immunoblotting. Out of more than 36 components separated by electrophoresis, transblotted to nitrocellulose sheet, and reacted with sera that showed a positive reaction to A. fumigatus or other fungal antigens on immunodiffusion tests, merely four or so were found useful to serodiagnosis of aspergillosis. An antigen of 82KD was found most reactive and specific component so as to be contained in the standard preparation. Several other components, for example 11KD, 26KD, 30KD and 31KD, also possessed relatively high reactivity and specificity and seemed to be worth while purifying and characterizing. Antibody binding activity (reactivity) of the antigenic components was clearly shown on immunoblots because some were faintly stained with Coomassie blue but darkly stained on immunoblots, while some others behaved contrary to them. A number of components seemed to carry not only species specific but cross reactive antigenic determinants. Immunoblotting proved very useful to identify serologically reactive and specific components that should be present in the antigen to be employed to the serodiagnosis of aspergillosis.
Chromosomal Studies of Fusarium oxysporum and its formae speciales (II)
Min, Byung-Re ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 76~81
The mitotic nuclear divisions in hyphae and chromosome number in 10 strains of Fusarium oxysporum were studies with the aid of Giemsa-HCl techniques. The chromosome number of fungi was ranged from 4 to 8. Of the 10 strains (F. oxysporum f. sp. lycoperici, F. oxysporum Kangnung D2) are n=4; two (F. oxysporum Sachun3, F. oxysporum S Kohung D2) n=5; five (F. oxysporum S Kohung 3, F. oxysporum CS Hongchun D16, F. oxysporum S Bosung 5, F. oxysporum SSunchun4 and F. oxysporum S Haenam 4) n=7 and one (F. oxysporum from the Australia) are n=8. These results along with my previous papers indicate that the basic chromosome number of the F. oxysporum may be n=4 and may have been evolutionary modification within this fugal group through diploidy and aneuploidy. As additional strains are studied, the chromosome number should help to reveal steps possible phylogenetic relationship within the group as well as more clearly defining taxonomic group and variation factors.
Homologous and Heterologous Antibody Response of the Patients with Aspergillosis Against Young Mycelia of Aspergilli by Fluorescence Antibody Reaction
Moon, Hi-Joo ; Kwon, Hyuk-Han ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 82~90
Detection of antibody against pathogenic fungi in serum specimens of the patients with pulmonary tuberculosis or other lung diseases has been carried out(male) using the indirect fluorescence antibody technique and immunodiffusion tests. Immunodiffusion tests revealed that 104(36.5%) out of 285 patients examined showed a positive precipitin reaction against one or more of fungal antigens. The majority of ID positive patients 64(61.5%) reacted with Aspergillus fumigatus antigen and 49(47.1%) patients reacted with Candida albicans antigen ID positive reaction to A. fumigatus was found little more frequently among male patients, while Candida albicans reactors were found more frequently among female patients. Age distribution of ID positive reactors was high(49.1-43.3%) in age group of 40-59 years, but least or none in age group of less than 30 years. Age of fungal mycelium used as antigen did not effect sensitivity of the indirect flubrescence (IF) technique in detecting antibody to A. fumigatus. Antibody class against A. fumigatus that showed highest titer was IgG and thus FITC labeled anti-IgG immunoglobulin shoul be preferable. As relatively large amount of cell wall components of Aspergilli shared antigenically, a considerable cross-reaction was observed among A. fumigatus, A. flavus and A. niger, but not much with C. albicans. While (IF) has much better sensitivity when compared with ID, relative specificity of the latter procedure cannot to be overried, so that they could be batter used together in order to obtain quantitative measurement of antibody with relative specificity.
Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (III) -Light- Induced Mitochondrial ATP Synthase in the Lentinus edodes (Berk.) Sing.-
Min, Tae-Jin ; Lee, Wan-Gie ; Park, Sang-Shin ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 91~98
Mitochondria in the L. edodes was separated and purified by stepped sucrose density gradient centrifugation. The activity of mitochondrial ATP synthase has been investigated during various illumination times at each wavelength within the range of 400 nm to 700 nm. The stimulation of above activity increased by two times compared with nonilluminated control group when the illumination was given for 15 seconds at 470 nm wavelength. The optimal pH and temperature of this light-induced mitochondrial ATP synthase were 7.5 and
, respectively. The activity of this enzyme increased by 26%, 25% and 14%, respectively, when there were 1 mmole
, 0.5 mmole
, and 5 mmole
ion, and was inhibited by 5 mmole
, 5 mmole
, 1 mmole
, 0.1 mmole
, 5 mmole
, and 0.1 mmole
ion did not affect the activity of enzyme.
Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (IV) -Effect of organic compound and metal ion influx of light-induced Mitochondrial ATP synthase in Lentinus edodes (Berk.) Sing-
Min, Tae-Jin ; Lee, Wan-Gie ; Kim, Jae-Woong ; Mheen, Tae-Ick ;
The Korean Journal of Mycology, volume 17, issue 2, 1989, Pages 99~104
Effects of organic compounds, photosensitizers and influx of metal ions on the light-induced mitochondrial ATP synthase in Lentinus edodes purified by stepped sucrose density gradient centrifugation were studied. In our previous work, the activation wavelength and the illumination time of mitochondrial ATP synthase were 470 nm and 15 sec, respectively. This enzyme was activated 85% by 1 mmole 2,6-dichlorophenol indopheol and inhibited by 1 mmole 2,-4-dinitrophenol,
oligomycin per ml of ethanol. Particularly, the enzyme was activated 414% by 10 mmole phenazine methosulfate as photosensitizer at 470 nm light. In the influx effects of
ion, the activity of the above enzyme increased under the optimal light condition compared with nonillumination state.