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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 17, Issue 4 - Dec 1989
Volume 17, Issue 3 - Sep 1989
Volume 17, Issue 2 - Jun 1989
Volume 17, Issue 1 - Mar 1989
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Synthesis of Resin Derivatives and Purification of Protein - Synthesis of p-Aminoanilinylsuccinyl-AH-Sepharose 4B and Purification of Protein in Pleurotus cornucopiae -
Min, Tae-Jin ; Kim, Yong-Rip ; Park, Sang-Shin ; Lee, Soo-Yong ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 105~113
For selective purification of proteins in Pleurotus cornucopiae, affinity chromatography was performed by p-aminoanilinylsuccinyl-AH-Sepharose 4B gel synthesized by treating p-phenylene diamine with succinyl-AH-Sepharose 4B, which was prepared by treating AH-Sepharose 4B with succinic anhydride. The capacity of p-aminoanilinyl ligand group was 6.1 micromole per milliliter of gel. Total apparent molecular weight of the affinity proteins eluted from the synthesized gel was 167 KD, which were a protein complex of 130 KD and 37 KD. The contents of the nonpolar, polar, positively and/or negatively charged amino acids in the affinity protein were 44.57%, 24.75%, 21.25%, and 9.43%, respectively. Total apparent molecular weight of the affinity proteins eluted from the AH-Sepharose 4B gel was 95.2 KD, which were a protein complex of 61 KD, 31 KD and 3.2 KD. The contents of the nonpolar, positively and/or negatively charged amino acids in the affinity protein by AH-Sepharose 4B gel were 44.05%, 29.13%, and 12.91% respectively.
Transfer of Isolated Nuclei from Agrocybe aegerita Mycelia into Pleurotus florida Protoplasts
Yoo, Young-Bok ; Shin, Pyung-Gyun ; Kim, Han-Kyoung ; Byun, Myung-Ok ; You, Chang-Hyun ; Cha, Dong-Yeul ; Chang, Kwon-Yawl ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 114~118
The transfer of isolated nuclei from Agrocybe aegerita mycelia of wild type into Pleurotus florida protoplasts of auxotroph was induced with polyethylene glycol. The type 1 of nuclear transfer products was spontaneous segregants of both parental morphology of colony. Hyphae of A. aegerita type had clamp connections while that of P. florida type lacked. Type 2 was main products of nuclear transfer which formed true clamp connections. Type 3 was clampless products. They all produced primordia and developed basidiocarps similar to Agrocybe aegerita. A comparison of nuclei transfer products was made using isozyme analyses of alcohol dehydrogenase, esterase, lactate dehydrogenase and peroxidase. Isozyme band patterns of some type 2 strains produced a new band. Band patterns from mycelial extracts of the other strains could be characterized by parental bands.
Interorder Protoplast Fusion between Pleurotus ostreatus and Ganoderma applanatum
Yoo, Young-Bok ; Song, Moon-Tae ; Go, Seung-Joo ; You, Chang-Hyun ; Cha, Dong-Yeul ; Park, Yong-Hwan ; Chang, Kwon-Yawl ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 119~123
Interorder heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of Pleurotus ostreatus in agaricales and Ganoderma applanaturm in aphyllophorales. When transferred to MMM plates, all fusion colonies exhibited an extremely growth rate. During three times subcultivation on MCM the growth rate of fusants showed faster little by little. Seventy-five % fusion products of potoplasts showed mixed morphologies between those of P. ostreatus and G. applannatum in the first subcultivation on MCM and MGM. The phenotype of these fusants changed similar those of P. osteatus type after three times subcultivation on MCM. However, phenotype of 25% stable strains did not change on subcultivation. Hyphae of all fusion products did not form true clamp connection. All these types did not produce primordia. A comparrison of interorder somatic hybrids was made using isozyme analysis of esterase, malate dehydrogenase and peroxidase. In most cases the enzyme patterns of G. applanatum were not distinct, however, fusant showed non-parental bands.
Studies on the Artificial Cultivation of Agrocybe aegerita (Brig) Sing using Pine Sawdust Substrate
Kim, Han-Kyung ; Park, Jeong-Sik ; Kim, Yang-Sup ; Cha, Dong-Yeul ; Park, Yong-Hwan ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 124~131
Some factors effecting on mycelial growth and cultural substrates using pine sawdust were investigated to develop an artificial cultivation technique. The optimal temperature and media pH for the mycelial growth were at
and 4.0, respectively. The mycelial growth was not different among three isolates tested. Among them ASI 19003 isolate showed the highest mycelial density and fruitbody yield. Among supplements added into pine sawdust, wheat bran was the best and its appropriate percentage was 20 for the mycelial growth. The mycelial densities were uprisen according to the amounts of the supplement. The highest yield, 107g/bottle of the sporophores of the mushroom was obtained from the substrates supplemented by 30% of wheat bran, but no fruitbody was formed on non supplemented one. Optimal moisture contents of the substrates was different for the mycelial growth, pinheading and fruitbody yields. Sixty five, 60 and 75% of moisture contents of the substrates were optimal for the mycelial growth, pinheading and fruitbody yields, respectively.
The Flora of Higher Fungi in Mt. Jiri Areas (III)
Park, Seong-Sick ; Cho, Duck-Hyun ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 132~136
Higher Fungi were collected in Mt. Jiri National Park areas from May, 1987 to October, 1988. These higher fungi were identified. As a result of identification, Boletellus linderi Sing., Lactarius corrugis Peck, L. gerardii var. gerardii Hesler & Smith, L. gerardii var. fagicola Hesler & Smith and L. subgerardii Hesler & Smith were newly recorded in Korea.
Effects of Incompatibility on Protoplast Fusion between intra-and inter Species in Basidiomycete, Pleurotus spp.
Go, Seung-Joo ; You, Chang-Hyun ; Shin, Gwan-Chull ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 137~144
Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by Poly ethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed mutants.
Purification and Characterization of Acid-stable
of Aspergillus niger K-25
Cho, Myung-Hwan ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 145~148
produced by Aspergillus niger K-25 strain was purified by fractional precipitation with ammonium sulfate, ethacridine and acetone. The final preparation was homogeneous in cellulose acetate electrophoresis. The enzyme retained 91 % of its oringinal activity at pH 3.0, 8.7% at pH 2.4. The optimum pH of the enzyme was around pH 4. The purified-enzyme with optimum temperature of
was more heat-stable than the commercial product. The enzyme retained 80% of its original activity when heated to
for 30 minutes while the commercial amylase lost its acitivity completely within 30 minutes at
Isolation of Aspergillus niger K-25 Prroducing Acid-stable
Cho, Myung-Hwan ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 149~153
One strain of Aspergillus niger K-25 producing an acid-stable
was isolated from the soil. The optimum culture conditions were investigated. The production of the acid-stable
was enhanced when the strain was incubated in a medium containing soluble starch 3.5%, peptone 2%,
1.0% at pH 3 for 7 days. However, higher activity of acid-stable
was demonstrated on wheat bran culture. Amylase production was doubled when A. niger K-25 was incubated on the wheat bran supplemented with fumaric acid buffer (pH 3).
Construction of Intraspecific Hybrids by Nuclear Transfer in Aspergillus nidulans
Yang, Young-Ki ; Park, Yeol ; Rhee, Young-Ha ; Maeng, Pil-Jae ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 154~160
The nuclear transfer technique was employed to obtain intraspecific hybrids in Aspergillus nidulans. Nuclei isolated from either a wild type or an auxotrophic mutant strain (FGSC 475) were transferred into the protoplasts of a recipient strain (FGSC 514). The frequency of hybrid formation (4.8% and 10.1 %, respectively) by nuclear transfer was higher than the frequency (0.6%) by protoplast fusion. Furthermore, most of the hybrids formed showed increased activity of some components of cellulase system, xylanase system, and mannanase. The hybrids were analyzed to be either diploid or aneuploid. These results suggest that nuclear transfer technique is more efficient the formation of intraspecific hybrids than protoplast fusion method and is useful for the improvement of Aspergillus strains.
Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (V) - Effects of FAD and
on Light-Induced Mitochondrial ATPase and ATP Synthase in Lentinus edodes -
Park, Sang-Shin ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 17, issue 3, 1989, Pages 161~168
Mitochondria in L. edodes were separated and purified by stepped sucrose density gradient centrifugation. In our previous work, we have found that the activation wavelengths of the mitochondrial ATPase and ATP synthase were 680 nm and 470 nm within the range of 400-700 nm, respectively. The activities of the above enzymes with wavelengths of 300-400 nm region were investigated. The mitochondrial ATPase and ATP synthase were stimulated at 380 nm and 330 nm, respectively, for 30 min illumination compared with dark control group. They, however, were inhibited at 330 nm and 350 nm, respectively. The presence of FAD resulted in inhibition of the activity of the ATPase and stimulation of the activity of the ATP synthase by the activation and inhibition wavelengths. However, the activities of these enzymes were not changed by NADH for the above wavelengths. In the spectral properties, the oxidation of
into FAD occurs in the presence of the enzymes for illumination of the activation and inhibition wavelengths. Therefore, we can predict that the mitochondrial ATPase and ATP synthase may function as oxidant in the redox reaction by the light illumination and that the light-induced pigment of the mitochondrial ATP synthase should be an oxidized form of a flavoprotein.