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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 18, Issue 4 - Dec 1990
Volume 18, Issue 3 - Sep 1990
Volume 18, Issue 2 - Jun 1990
Volume 18, Issue 1 - Mar 1990
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Studies on Protoplast Formation and Regeneration of Coriolus versicolor
Bok, Jin-Woo ; Park, Seol-Hee ; Choi, Eung-Chil ; Kim, Byong-Kak ; Yoo, Young-Bok ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 115~126
To establish basic techniques for protoplast fusion of Coriolus versicolor several factors affecting protoplast formation and regeneration were investigated. Protoplast isolation was at maximum with 2.5-day cultured mycelia of C. versicolor treated with the combination of two enzymes, Novozym 234 (10 mg/ml) and cellulase Onozuka R-10 (15 mg/ml), for 3-4.5 hours at
As an osmotic stabilizer for stabilizing the protoplast, 0.6 M sucrose was the best for formation and regeneration of the protoplast from the mycelia of the fungus and the regeneration frequency was 3.48%. Protoplast fusion was made by a modified method of Peberdy using PEG (M.W. 4,000). The fusion frequency between two mutants of C. versicolor was 1.86% and the fusion products showed differences in growth rate and colony morphology.
A Diversity of Complexipes moniliformis in Korea
Lee, Sang-Sun ; Ka, Gang-Hyen ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 127~131
Various types of Complexipes moniliformis were found at two communities of Cassia minosoides var. nomame and lichen mixed with an unidentificable plant in Tae Ahn Kun (Chung Nam). The morphological features observed at the chlamydospore of Complexipes moniliformis were not significant, but the size of chlamydospores was quite different in the statistic analysis. The morphological features affected by environmental or genetic factors were discussed.
Chromosomal Study on the Genus Fusarium
Min, Byung-Re ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 132~136
The vegetative nuclear divisions in hyphae and the chromosome of Fusarium were observed by use of HCI-Giemsa technique and light microscope. The chromosome of nuclear in F. moniliforme both 7150 and 7219 were eight. F. subglutinans 1082 was n=8 and n=7 in F. subglutinans 1083. F. nygamai 5668 was n=7 and n=5 in F. nygamai 7132. F. beomiforme 9758 and 9760 were n=7. F. coccidicola ATCC 24138 and F. acuminatum ATCC 16560 were n=6. From these results and other reports, the basic chromosomal number of these fungi might be speculated to be four.
Effects of Alkali Extract of Ganoderma lucidum IY007 on Complement and Reticuloendothelial System
Lee, June-Woo ; Jeong, Hoon ; Chung, Chun-Hee ; Lee, Kweon-Haeng ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 137~144
To examine effects on complement and reticuloendothelial system, alkali extract was isolated from cultured mycelium of Ganoderma lucidum IY107. It was shown to strongly activate both classical and alternative pathways of complement as compared with krestin. Activated complement C3, 3rd peak, was observed by crossed immunoelectrophoresis. It was also shown to activate reticuloendotherial system of ICR mice in the carbon clearance test and to increase hemolytic plaque forming cells of the spleen. Carbohydrate and protein contents of the alkali extract were 10% and 49%, respectively. The carbohydrate consisted of four monosaccharides and the protein contained 16 amino acids.
Studies on the Anticomplementary Activity of Korean Higher Fungi
Jeong, Hoon ; Lee, June-Woo ; Lee, Kweon-Haeng ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 145~148
Anticomplementary activity of 61 strains of Korean higher fungi was screened for immunostimulation. Extracts from 11 of 61 strains including 5 of Ganoderma lucidum, 3 of Lentinus edodes, 2 of Cordyceps sp. and 1 of Agaricus campestris, showed higher anticomplementary activity than krestin which was immunopotent extract from Japanese Coriolus versicolor. The most potent anticomplementary activity was found with extract from Lentinus edodes IY105 whose complement consumption was 31.7%.
Studies on the Mycelial Cultivation and the Rhizomorph Production of Armillaria mellea
Hong, Jai-Sik ; Kim, Myung-Kon ; So, Gyu-Ho ; Kim, Young-Hoi ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 149~157
Five strains of Armillaria mellea were collected from the forest of Chonbuk province and isolated from the tissue of fruit bodies. Nutritional and environmental characteristics of mycelial cultivation and rhizomorph production of Armillaria mellea isolated were determined in sawdust media, woody inocula and soils. The sawdust media of Styphnolobium japonicum, Culhamia simplex, Populus monilifera and Populua davidiana were proper for mycelial growth. The ranges of optimum pH, temperature and moisture content for mycelial growth were in the range of
, respectively. Among the various additives and inorganic salts added, 10% rice bran and 3%
were effective to mycelial growth. The woody inocula of Styphnolobium japonicum, Culhamia simplex, Quercus acutissima and Quercus veriabilis were proper for rhizomorph production. The ranges of optimum pH, moisture content and temperature for rhizomorph production were in the range of
, respectively. Distribution of rhizomorphs in soil was varied with depth, but the main concentration occurred in the range of
. They were rarely found below 25.0 cm.
Alcohols and Volatile Organic Acids as Stimulants of Rhizomorph Production by Armillaria mellea
Hong, Jai-Sik ; Kim, Myung-Kon ; Lee, Jai-Hong ; Kim, Hyung-Moo ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 158~163
To investigate symbiotic relationship of 'Chunma (Gastrodia elata) and the rhizomorph of Armillaria mellea, volatile organic acids and alcoholic compounds which were considered to be contained in Gastrodia elata were tested to determine stimulatory effects on rhizomorph growth on a chemically defined medium. Also, volatile organic acids were isolated from Gastrodia elata and analyzed by gas chromatography. The growth of rhizomorph was stimulated by the presence of alcohols and volatile organic acids, but acetic acid and methanol were ineffective. In the presence of valeric acid and ethanol, Armillaria mellea produced abundant rhizomorph at concentrations of 0.1 and 1%, respectively. Ethanol and valeric acid supplemented at regular intervals of 3 days as lower concentrations in the medium stimulated the growth of Armillaria mellea. The concentrations of ethanol and valeric acid as low at 0.01% added 3 days intervals for 15 days were more effective than initial concentrations of 0.1 and 1% in stimulating rhizomorph development of Armillaria mellea. Eight kinds of volatile organic acids were identified and quantified by gas chromatography. The major compounds were n-propionic, valeric, iso-carproic and caproic acids, and the minor compounds were iso-butyric, butyric, iso-valeric and hepatanoic acids. Valeric acid was the most abundant among them.
Nature of Suppressiveness and Conduciveness of Some plant pathogens in Soils
Shim, Jae-Ouk ; Lee, Min-Woong ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 164~177
This study was carried out to obtain some useful data for increasing an effective ginseng production. There was a direct relationship (r=0.2645) between spore germination of Fusarium solani and soil pH, and (r=0.315) between Cylindrocarpon destructans and soil pH. On the other hand, there was a direct relationship (r=0.19) between relative hyphal growth of Rhizoctonia solani and soil pH. There was a direct relationship (r=0.21) between number of total bacteria and F. solani, (r=0.37) between actinomycetes and F. solani and (r=0.20) between celluloytic bacteria and F. solani. However, there was an inverse relationship (r=-0.20) between number of total fungi and F. solani. There was a direct relationship (r=0.24) between number of actinomycetes and R. solani. Each ginseng pathogen-suppressive soil screened was 40 in F. solani, 20 in C. destructans and 9 soil samples in R. solani among 146 soil samples, respectively. The mean contents of K, Ca and Mg were fairly lower in each ginseng pathogen-suppressive soil than conducive soil, whereas Na were somewhat lower. The mean contents of organic matter were over 2 times higher in each ginseng pathogen-suppressive soil than conducive soil. The mean contents of phosphate were fairly lower in F. solani and R. solani-suppressive soil than conducive soil and, on the other hand, were somewhat higher in C. destructans-suppressive soil than conducive soil. The mean soil pH was somewhat lower in each ginseng pathogen-suppressive soil than conducive soil. The mean contents of sand were about 2 times higher in each ginseng pathogensuppressive soil than conducive soil, whereas silt and clay were somewhat lower. The microbial numbers of total bacteria, total fungi and celluloytic fungi were higher in F. solani-suppressive soil than conducive soil, whereas actinomycetes and celluloytic bacteria were lower. Each microbial number of total bacteria or total fungi indicated a significant difference (p=0.05) between F. solanisuppressive and conducive soil, and the microbial number of actinomycetes was a highly significant difference (p=0.01) between F. solani-suppressive and conducive soil. The microbial numbers of total bacteria, total fungi, actinomycetes and celluloytic fungi were higher in C. destructans-suppressive soil than conducive soil, whereas celluloytic bacteria were about 2 times lower. On the other hand, the microbial numbers of total fungi were higher in R. solani-suppressive soil than conducive soil, whereas total bacteria, actinomycetes, celluloytic bacteria and celluloytic fungi were lower. Fourteen of 16 F. solani-suppressive soils tested were suppressive to ginseng root rot, whereas fifteen of 16 C. destructans-suppressive soils were suppressive. Ginseng root rots of ginseng disease-suppressive soils were in the range of 1.0-17.4% in F. solani-suppressive soil and 0.2-20.4% in C. destructans-suppressive soil, respectively.
New Description of Acaulospora scrobiculata Collected in Korea
Ka, Kang-Hyeon ; Lee, Sang-Sun ; Lee, Min-Woong ;
The Korean Journal of Mycology, volume 18, issue 3, 1990, Pages 178~180
New description of Acaulospora scrobiculata was conducted. based on the azygospores collected from the soils of four different plant communities in Korea.