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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 21, Issue 4 - Dec 1993
Volume 21, Issue 3 - Sep 1993
Volume 21, Issue 2 - Jun 1993
Volume 21, Issue 1 - Mar 1993
Selecting the target year
A study on hyphomycetous fungi found on Maejus, a raw material of Korean traditional soysources
Lee, Sang-Sun ; Park, Kwang-Ho ; Choi, Kyoung-Jin ; Won, Sun-Ae ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 247~272
Forty-eight fungal isolates were collected from the Korean traditional maejus and twenty-eight isolates of them were identified to be a hyphomycetous fungus(three genera and 20 species) Aspergillus flavus, A. flavus var columnaris, A. oryzae, A, oryzae var effusus, A. terreus, Scopulariosis brevicaulis, Penicillium botryosum, P. gorlenkoanum, P. griseo-purpureum, P. citrinum, P. miczynskii, P. gaditanum, P. turolense, P. funiculosum, P. rubicundum, P. godlewskii, P. jensenii, P. roqueforti, P. volguense, P. verrucosum. Various 14 species of Penicillium were isolated from maejus, but all of them were not considered to be involved in the maeju fermentation. Otherwise, S. brevicaulis was especially collected from the maejus of various located areas, but were also related to the good quality of maejus under labratory conditions. A. flavus, A. oryzae P. tulolense, and P. funiculosum were also observed to be involved in the process of maeju.
Studies on Inorganic Components of Korean Wild Edible Mushrooms - Trace Mineral Elements of Armillariella Mella, Hygrophorous russula, Armillariella tabescens, Lepista nuda and lepista sordida, Hygrocybe conica. -
Park, Wan-Hee ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 273~278
In order to determine of trace mineral elements of wild edible mushrooms in Korea, the dried carpophores of Armillariella mella(Vahl.ex Fr.) karst., Armillariella tabescens(Scop.) Sing., Lepista nuda(Bull.ex Fr.) Cooke and Lepista sordida(Schum.ex Fr.) Sing., Hygrophorus russula (Schaeff.ex Fr.) Qu'el., Hygrocybe conica(Scop.ex Fr.) Kummur were incinerated and analyzed by an atom adsorption spectrophotometry. The six mushrooms contained ubiquitously potassium, iron, zinc, sodium, manganase, copper and calsium in that order, and the content of potassium was the highest than other inorganic components and the content of zinc in Hygrocybe conica was especially the most than the other five mushrooms. Total contents of inorganic components(except Cd) were universally the highest in Hygrocybe conica than other five mushrooms, and decrease in order Hygrophorus russula, lepista nuda, lepista sordida, Aarmillariella mella and Armillariella tabescens. Calsium was not present in Amillariella mella and lepista nuda, and copper not in Hygrocybe conica, and content of cadimium in Hygrophorus russula and Hygrocybe conica were trace.
Cloning and Expression of the metE gene coding for homocysteine methyltransferase from the basidiomycete Ganoderma lucidum in E. coli
Kim, Hyun-Jeong ; Park, Dong-Chul ; Lee, Kap-Duk ; Lee, Byul-La ; Lee, Kap-Rang ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 279~284
The metE gene coding for
homocysteine methyltransferase from the basidiomycete Ganoderma lucidum was cloned by complementation of methionine-requiring mutants of E. coli. The size of a inserted DNA was about 1.54 kb and had 5 restriction enzyme sites. A physical map was constructed. Southern blot analysis confirmed the presence of a transforming DNA in the genome of Ganoderma lucidum. indicating the presence of a single copy.
Fermentative Production of 5'-GMP from 5'-XMP by XMP aminase and ATP-generation System of Saccharomyces cerevisiae
Cho, Jung-Il ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 285~292
For the enzymatic conversion of 5'-XMP to 5'-GMP, partially purified XMP aminase from Escherichia coli was coupled with the yeast, Saccharomycrs cerevisiae, capable of ATP regeneration through glycolytic pathway. In order to elevate the level of XMP aminase in E. coli,
mutant were introduced, and the yeast used as ATP supplier was treated by some method to increase its membrane permeability. The optimum conditions for efficient conversion reaction by energy-coupled system were investigated. As the results, a CH 41,
mutant of E. coli K-12, showed 2.75 fold increase in the level of XMP aminase, compared with its parent cell. And the lyophylized yeast was the most effective at the ATP supplier. The optimum temperature and pH of conversion reaction were
and pH 7.4, and the highest conversion ratio was shown under the reaction condition of 100 mM glucose, 100 mM inorganic phosphate and 6 mM AMP. When 36 units/ml XMP aminase used under the above conditions, the amount of 60 mg/ml yeast was sufficient to be used. Under the optimum condition, 71% of 1.8 mM(65.6 mg/100 ml) 5'-XMP was converted to 5'-GMP within 8 hr.
Comparisons of Isozyme Patterns in Pythium Species and Application to Pythium Systematics
Lee, Youn-Su ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 293~300
For the enzymes AAT, GmDH, ME, GPI, LDH and IDH, nine, seven, four, nine, seven, and four different phenotypes, respectively, were observed. All six isolates of an unidentified sterile Pythium sp. isolated from field soil showed the same band positions for all six enzymes compared. These phenotypes were not similar to any of the known Pythium species. Two isolates of unknown Pythium species (145 and 299) showed the same band positions for all six enzymes. The phenotypes for all three unknown Pythium spp. were different from the other species in the experiment. Five isolates of P. heterothallicum showed the same band positions for all enzymes compared except one enzyme, lDH. Two isolates of P. torulosum showed the same band petitions for enzymes AAT, GmDH and ME, and three isolates of P. totulosum showed the same positions for enzymes GPI, LDH, and IDH. Single isolates of P. spinosum and P. irregulare showed the same band positions for enzymes AAT, GmDH and GPI. In conclusion, sterile types of Pythium species showed 100% similarities among themselves but did not show any similarity with all isolates of P. heterothallicum and P. spinosum isolate, and showed very low similarities with other isolates in general except with unknown Pythium isolate 306. Similarity levels between different species were low in general with few exceptions.
Partial Purification and Some Properties of Carboxymethyl Cellulases from Alkalophilic Cephalosporium sp. RYM-202
Kang, Myoung-Kyu ; Park, Hee-Moon ; Rhee, Young-Ha ; Kim, Yun-Seog ; Kim, Yeo-Kyung ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 301~309
An alkalophilic Cephalosporium sp. RYM-202 capable of producing cellulase components was isolated from soil. This organism grew best at an initial pH 9.0 and produced cellulase maximal at an initial pH 9.5-10.0. Three carboxymethyl cellulases(CMCases), P-I-I, P-I-II and P-II-I, were partially purified by DEAE-Sephadex A-50 ion exchange column followed by Sephadex G-150 gel filtration. The optimum pH values for activity were 7.5 for P-I-I, 8.0-9.5 for P-I-II and 7.5-10.0 for P-II-I. All CMCases were stable between pH 4.5 and 12.0. Temperature optima for activity ranged between 40 and
and more than 50% of the maximum activity was observed at
for both of P-I-I and P-II-I. The activity of CMCases was significantly stable in the presence of various laundry components, such as, surfactants, chelating agents and alkaline proteinases.
Production of Ligninase in Agitated Submerged Cultures of Phanerochaete chrysosporium Diffuse Mycelia
Kim, Kyung-Soo ; Kim, Young-Ho ; Kang, An-Seok ; You, Chang-Hyun ; Cha, Dong-Yeul ; CROAN, SUKI C. ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 310~315
Phanerochaete chrysosporium is a white rot fungus which secrets a family of lignin-degrading enzymes under nutrient limitation. Ligninase was extracellularly produced in agitated submerged cultures of P. chrysosporium, SC 26. Addition of veratryl alcohol(4 mM), and benzyl alcohol(10 mM) with 0.1% Tween 20 to the culture medium stimulated ligninase production. However, ligninase was not detected when both treatments of veratryl alcohol and benzyl alcohol without Tween 20 were added to the medium. Addition of 0.1 % Tween 20 to the culture medium had little effect on ligninase activity. The ligninase activity was maximum on day 5-8 for veratryl alcohol, and benzyl alcohol with 0.1 % Tween 20 additive medium.
Colonization of Retama raetam Seeds by Fungi and Their Significance in Seed Germination
OUF, S.A. ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 316~322
Examination by scanning electron microscopy and potato-dextrose agar medium showed that the dry seeds of R. raetam were externally free of fungi. When planted in sandy loam soil, the seeds become colonized with eleven soilborne fungal species. The fungi were isolated on cellulose agar, pectin agar and lignin agar media. Aspergillus flavus, A. niger, Penicillium capsulatum and Fusarium oxysporum had broad occurrence and recovered on the three media. The production of hydrolytic enzymes by the isolated fungi depends on the substrate and species. P. capsulatum, P. spinulosum and A. niger had wide enzymatic amplitude and they were able to produce cellulolytic, pectolytic and lignolytic activities on corresponding substrates as well as on seed coat containing media. The lignolytic activities of the isolated species except Chaetomium bostrychods and Trichoderma viride were enhanced on applying the seed coat materials as C-source rather than using lignin. Soaking R. raetam seeds in culture filtrates of the most fungi grown on seed coat supplemented media induced pronounced and distinct stimulating effect on seed germination. The most effective filtrates were those of P. capsulatum, P. spinulosum and Sporotrichum pulverulentum.
Intergeneric protoplast fusion between Gliocladium virens and Trichoderma harzianum
Shin, Pyung-Gyun ; Cho, Moo-Je ;
The Korean Journal of Mycology, volume 21, issue 4, 1993, Pages 323~331
The protoplast formation and intergeneric protoplast fusion between Gliocladium virens and Trichoderma harzianum were attempted to obtain fusants. Protoplast formation was the most effective when the strains were treated with concentration of 5 mg/ml of Novozyme 234 and Cellulase at
for 3 hours in phosphate buffer, pH 6.5, supplemented with 0.6 M sorbitol as osmotic stabilizer. Auxotrophic mutants of G. virens G88 did not grow in minimal medium and benomyl resistant T. harzianum T95 from wild types, however, was selected by treatment with UV light as genetic marker to isolate fusants. When the intergeneric protoplast fusion between G. virens G88 and T. harzianum T95 was carried out using 30% PEG 4000 containing 10 mM
, and 50 mM glycine (pH 8.5) as fusogenic agent at
for 10-15 min, the fusion frequency was
. Fusants obtained from intergeneric protoplast fusion were spontaneously segregated into va rious strains by continous culture on complete medium. Several intergeneric hybrids were classified into three types: parent-like hybrids, segregants, and recombinants.