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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 22, Issue 4 - Dec 1994
Volume 22, Issue 3 - Sep 1994
Volume 22, Issue 2 - Jun 1994
Volume 22, Issue 1 - Mar 1994
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Isolation and Characterization of NSD mutants in Aspergillus nidulans
Han, Dong-Min ; Han, Yoo-Jeong ; Kim, Jee-Hyun ; Jahng, Kwang-Yeop ; Chung, Yoon-Shin ; Chung, Jae-Hoon ; Chae, Keon-Sang ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 1~7
Several mutants which never underwent to sexual development(NSD) of Aspergillus nidulans were analyzed genetically and physiologically. They were divided into two groups according to their characterisitics of asexual development after release from aeration block. The mutants in first group proceeded asexual development immediately after removal of aeration block, while those in second group did 10 hours or more later. The NSD mutants were separated into 4 complementation groups, nsdA, nsdB, nsdC and nsdD. The nsdA and nsdD genes were linked to AcrA1 on linkage groupⅡ and pabaA1 on linkage group I, respectively. The mutant alleles were all recessive to wild type allele in heterokayon state. The mutants did not developed cleistothecia on any of carbon sources, except NSD208 which developed cleistothecia on lactose.
Effects of Carbohydrates on the Biosynthesis of Phospholipids and Fatty Acid Composition in Aspergillus, Rhizopus and Candida Cells
Cho, Sun-Hee ; Lee, Chong-Sam ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 8~30
The synthesis of phospholipids and the composition of fatty acids were analyzed in the fungal cells of Aspergillus phoenicis, Rhizopus acidus and Candida albicans treated with carbon sources (glucose, sucrose, raffinose) during the culture. Growth ratios were predominantly in Aspergillus and Candida treated with sucrose and those in Rhizopus treated with glucose. The synthesis of total lipids were accelerated in Aspergillus and Rhizopus with glucose and the contents of total lipid was increased in Candida with raffinose. The glucose treatment increased Phosphatidylinositol(PI) content by 73.7% for Aspergillus and Phosphatidylcholine(PC) content by 292% for Rhizopus. In sucrose treatment the synthesis of PI was accelerated 112% for Aspergillus and that of PC was increased 77.7% for Rhizopus and 71.8% for Candida. In raffinose treatment, the content of PC was increased 79.5% for Aspergillus, the biosynthesis of Phosphatidylethanolamine(PE) was inhanced 50.9% for Rhizopus and 49.1% for Candida. In Aspergillus and Candida, oleic acid and linoleic acid were major fatty acids for biosynthesis of PC and PE when the three carbon sources were treated. The major fatty acids for biosynthesis of phospholipid was palmitic acid and linoleic acid in Aspergillus, palmitic acid in Rhizopus and oleic acid in Candida. Palmitic acid was one of major fatty acids utilized for the biosynthesis of phospholipid(PC, PE, PI) in the fungal cells treated with carbohydrates.
Cell Biological Changes of Validamycin Resistant Strain in Coprinus cinereus
Shim, Jae-Yong ; Choi, Hyoung-Tae ; Yoon, Kwon-Sang ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 31~35
Coprinus cinereus resistant strain against validamycin was successfully isolated. Germination rate of oidiospores, branching pattern, and localization of actin protein of the resistant strain were compared with normal strain. The resistant strain showed better germiantion rate of oidia (about 20 fold), more frequent branching, and even actin localization on validamycin plate where actin content was severely reduced in case of normal strain.
Hyphal growth, auxiliary cell development and hyphal healing process of arbuscular mycorrhizal fungi, Gigaspora and Scutellospora genera
Ka, Kang-Hyeon ; Koo, Chang-Duck ; Yi, Chang-Keun ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 36~45
Hyphal growth, auxiliary cell development and hyphal healing process of Gigaspora margarita, Scutellospora heterogama and S. verrucosa were investigated. The germinated hyphae from spores grew on the surface and the bottom of agar media. The hyphal growth on the surface stopped 19 to 23 days and the growth on the bottom 40 to 51 days after spore germination. Auxiliary cells began to develop 7 to 9 days after the spore germination in the media. The auxiliary cells almost always developed on the tip of a hypha branched from a secondary hypha. The cytoplasmic streaming rates in the hyphae of G. margarita and S. heterogama were
, respectively. The hyphae artificially cut were healed by connecting with a hypha grown from the spore-side hypha. We may suggest that the wound healing process of hyphae should be one of the characteristics obtained from symbiotic relationship between host plants and arbuscular mycorrhizal fungi for a long period of time.
Regulation of membrane-associated laccase synthesis in liquid culture of Coprinus congregatus
Choi, Yong-Ok ; Ha, Eun-Soo ; Kim, Soon-Ja ; Choi, Hyoung-Tae ; Yoon, Kwon-Sang ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 46~49
When Coprinus congregatus was cultivated in low pH YpSs medium(pH 4.2), the culture supernatant turned brown earlier than that of normal(pH 7.1) medium resulted from the melanization synthesized by the secreted laccase reaction. The pH of medium became 5.2 after 24 h incubation. Laccase which was deeply related to the fungal development might also be implicated in the neutralization of excess hydrogen ions to protect from acidification of cytoplasm.
Establishment of rhizosphere microbes for plant protection on soil-borne diseases -Benificial antagonist and its mode of action toward ginseng root rot pathogen-
Kim, S.I. ; Lee, M.W. ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 50~61
From soil samples, 380 antagonistic microorgnisms were isolated. Among the isolates, 42 strains had mycelia growing inhibition ability against Fusariun solani, ginseng root rot causing pathogen. Isolates CHA 1 and S-PFHR 6 were proposed as antagonists for this study and they were identified as Promicromonospora sp. and Pseudomonas pseudoalcaligenes respectively. As an antagonism against hyphae of F. solani in dual culture test, CHA 1 and S-PFHR 6 inhibited linear growing, caused abnormal branching, and the membrane projection which formed by cell wall destruction. The secondary metabolites contained in the culture filtrates which prepared from PD broth and Nutrient broth inhibited the spore germination to 14.3%. The culture filtrate of S-PFHR 6 which prepared by a little amount of soil extract addition to nutrient rich medium had more strongly. inhibited the spore germination and spore germination decreased to less than 4.0% in it. The soil used in this study had fungistasis and the germination rate of macroconidia and chlamydospore of F.solani was 19.4% and 17.7% respectively. The steam sterilized soil lost fungistasis and germination rate of conidia increased to more than 97.9%. The soils amended with the propagule of CHA 1 and S-PFHR 6 increased fungistasis and the germination rate of macroconidia decreased to 14.7% and 11.7% respectively in each treatments. But the soil ammended with glucose and asparagine annulled fungistatic ability and the germination rate of macroconidia increased to more than 48.0%. As an antagonistic activity of the secondary metabolites of two antagonistic isolates in soil, the germination rate of macroconidia of F. solani was 9.3% in the soil amended with the culture filtrate of CHA 1 but the culture filtrate of S-PFHR 6 had no such activity. In the soil which treated with antagonist propagule or culture filtrate, the chlamydospore germination rate was lower than that in natural soil. The addition of glucose and asparagine to antagonist propagule treated soil did not enhanced the chlamydospore germination.
Floral Studies on Korean Wood-rotting Fungi (II) -on the flora of the Aphyllophorales(Basidiomycotina)-
Jung, Hack-Sung ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 62~99
The fungal flora of Korean wood-rotting fungi were studied for two years from March of 1990 to February of 1992. Fresh fungi were collected from national parks, local areas, and islands throughout the country. Fleshy fungi of the Aphyllophorales were identified through specimen examination and literature studies. Total 217 species and 1 variety were counted, among which Aleurodiscus cerussatus, Botryobasidium obtusisporum, Ceraceomyces cystidiatus, Erythricium hypnophilum, Grandinia crustosa, Grandinia spathulata, Hyphoderma praetermissum, Hyphoderma roseocremeum, Hypochnicium bombycinum, Hypochnicium detriticum, Hypochnicium lundellii, Laeticorticium roseum, Mycoacia fuscoatra, Peniophora incarnata, Phanerochaete avellanea, Phanerochaete filamentosa, Phanerochaete martelliana, Phlebia lilascens, and Trechispora vaga under the Corticiaceae, Chondrostereum purpureum and Cystostereum subabruptum under the Stereaceae, Tomentella pilosa under the Thelephoraceae, Asterostroma laxum, Hymenochaete cruenta, Hymenochaete fuliginosa, Hymenochaete tabacina, Inonotus radiatus, and Phellinus pomaceus under the Hymenochaetaceae, Antrodia crassa, Antrodia serialis, Ceriporia reticulata, Oligoporus balsameus, Oligoporus guttulatus, Oxyporus cuneatus, Rigidoporus microporus, and Trichaptum laricinum under the Polyporaceae, total 36 species were confirmed as unrecorded species to Korea and are registered here with Korean names and English descriptions.
Taxonomical studies on Ascomycotina in Korea(I)
Park, Pyoung-Jae ; Lee, Ji-Yul ; Otani, Yoshio ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 100~106
Fifty eight specimens of Ascomycotina were collected at Gwangneung, Mt. Weorak, Mt. Seorak, Mt. Paldal, Mt. Sungsu, Mt. Chiri and Mt. Odae from May, 1989 to October, 1992. These Ascomycotina were identified and classified into 6 orders, 10 families, 19genera, and 29 species. Among them, Peziza michelii(Boud.) Dennis, Tricharina gilva(Boud.) Ekblad, Jafnea fusicarpa (Gerard) Korf, Scutellinia kergulensis(Berk.) O. Kuntze, Vibrissea truncorum Fries, Ciboria amentacea (Balbis ex Fr.) Fuckel, Hypoxylon fragiforme(Per. ex Fr.) Kickx and Nectria episphaeria(Tode ex Fr.) Fries were newly recorded in Korea. These species were described and their Korean names were designated.
Interorder Hybridization between Pleurotus ostreatus and Elfvingia applanata by Protoplast Fusion
Yoo, Young-Bok ;
The Korean Journal of Mycology, volume 22, issue 1, 1994, Pages 107~116
Interorder somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus in the order Agaricales and Elfvingia applanata in the order Aphyllophorales. The fusants were classified into stable heterokaryons and spontaneously segregated heterokaryons. Hyphae of all fusion products except two strains did not form clamp connections. Out of them, two clamped and three clampless fusants produced mature fruiting bodies by light-dark cycle on sawdust rice bran medium. All of these basidiocarps had clamp connections. Three fusants were analysed with the distribution of progenies and segregation of genetic characters by random spore analyses. The genetic markers were shown to segregate and recombine in the first generation of monospores isolated from basidiocarps. Phenotypes of a large number of auxotrophic progenies were not detected in the two clamped fusants. The aberration ratio of segregants indicated the gene interaction resulting from different genome structure between distantly related species. The polymerase chain reaction (PCR) was adopted for the detection of somatic hybrids nuclear DNA. Four fusants showed a positive results in three kinds of primers. The prominent reaction products are represented by new bands in primer # 87 and # 125. Out of four fusants, two somatic hybrids had non-parental mtDNA patterns when digested with EcoR1 and HindIII. Comparison of somatic hybrids, tissue culture isolates(TC) and multispore germination isolates(MS) were made using esterase isozyme analysis. It is apparent that somatic hybrids had a minor banding patterns which are quite different from those of parents.