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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 1994
Volume 22, Issue 3 - Sep 1994
Volume 22, Issue 2 - Jun 1994
Volume 22, Issue 1 - Mar 1994
Selecting the target year
Variations in Mitochondrial DNA of Pleurotus sajor-caju
Byun, Myung-Ok ; Kim, Kyung-Soo ; You, Chang-Hyun ; Cha, Dong-Yeul ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 117~121
Five strains of Pleurotus sajor-caju were collected from some countries including India and Papua New Guinea. Four strains produced brown fruitbody but the other strain from Papua New Guinea produced white fruitbody. Monokaryons obtained from both strains producing brown fruitbody and white fruitbody were mated each other. They showed different mating types such as brown strain of
and white strain of
. DNAs were isolated from mycelia of five strains of P. sajor-caju. Mitochondrial DNA was seperated from nuclear DNA by bisbenzimide-CsCl ultracentrifugation. Digestion of the fungal mitochondrial DNA with Eco RI restriction endonuclease yielded from ten to fourteen fragments. Two strains of the five strains showed different restriction pattern of mitochondrial DNA from the other three strains. Summation of the fragment sizes gave a mitochondrial DNA size of about 60 to 65kb.
Study on the Role of Metal ions for the Activity of the Mitochondrial
in Lentinus edodes
Park, Sang-Shin ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 122~129
The role of metal ions for the activity of the mitochondrial
was studied. Removal of non-heme iron ion from the mitochondria by dialysis against chelating agents, 10 mM ethylenediaminetetraacetic acid(EDTA) and 10 mM o-phenanthroline(o-Phe), led to 56% and 49% inactivation of the enzyme, respectively. The enzyme dialyzed against EDTA was reactivated 81% by the addition of 0.5 mM
and 70% by 0.5 mM
did not reactivate the enzyme. Coexistence of 0.5 mM
and 0.5 mM
resulted in 95% reactivation of the enzyme, while
with 0.5 mM
did not reactivate the enzyme like the effect of
alone. The enzyme dialyzed against o-Phe showed the similar results. These data showed that
is predominantly required for the activity of the mitochondrial
in Lentinus edodes and stimulated the activity of it by
increased enzyme's affinity for substrate, decreasing the Km value 1.67 mM to 0.65 mM.
Studies on Protoplast Formation and Regeneration of Lyophyllum decastes
Bok, Jin-Woo ; Kim, Jong-Pil ; Jin, Mi-Rim ; Choi, Eung-Chil ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 130~137
This experiment was carried out to investigate proper conditions for protoplast isolation and regeneration from mycelia of Lyophyllum decastes. Novozym 234(10 mg/ml) with 0.6 M
in phosphate buffer(pH 4.0) was proper for protoplast isolation. The optimal reaction time of the mycelium with the lytic enzyme was four hours in shaking condition at 120 strokes per min. When the mycelium of L. decastes was cultured at
for 5 days, the formation of protoplasts was effective. The liquid medium was more effective for protoplast isolation than the solid medium. In the liquid medium, high yields of protoplasts were obtained from 0.6 M
osmotic stabilizer. Protoplasts of L. decastes were regenerated to normal hyphal growth and the regeneration frequency of the protoplasts in the complete agar medium containing Triton X-100(0.0025%) was
. The regeneration medium stabilized with 0.6 M sucrose was the best for regeneration of the protoplasts. In contrast to protoplast formation, regeneration was inhibited by the inorganic salts used as osmotic stabilizer.
Isolation of Protoplasts from Rhizopus nigricans
Kim, Myung-Hee ; Kim, Mal-Nam ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 138~144
Conditions for isolation of protoplasts from spores and mycelia of Rhizopus nigricans were studied. Larger amount of protoplasts was obtained from swollen spores in liquid medium contained with 5% of 2-deoxy-D-glucose for 4 hours than from mycelia. Enzyme mixture of Novozym 234(2%) and
was most effective for the isolation of protoplasts from swollen spores and from mycelia. The solution of 0.6 M
or mannitol and pH 6.0 showed good results as the osmotic stabilizer and the optimal condition of pH of the enzyme solution for the isolation of protoplast from the swollen spores, respectively. At this condition,
of protoplasts was obtained from swollen spores by digestion with lytic enzyme mixture for 2 hours.
Study on the Artificial Cultivation of Lentinus lepideus(Fr. ex Fr.) Fr. -Investigation of Mycelial Growth Conditions-
Kim, Han-Kyung ; Park, Jeong-Sik ; Cha, Dong-Yeul ; Kim, Yang-Sup ; Moon, Byung-Ju ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 145~152
In order to investigate the cultural characteristics of Lentinus lepideus, media, pH, carbon sources, nitrogen sources, vitamin and organic acids were tested in submerged culture. It grew well in GPB(broth), and the optimum temperature and pH was
and 4.2, respectively. The carbon sources such as galactose belong to monosaccharide and maltose belong to disaccharide were effective for mycelial growth. Peptone which is compound nitrogen source was good for mycelial yield but the other nitrogen sources were not effective. Among the various vitamins, pyridoxine is suitable for mycelial yield. Among the various organic acids, citric acid promoted the mycelial yield but acetic acid interrupted the mycelial growth of L. lepideus.
Studies of Cultural Condition on the Mycelial Vegetative Growth in Naematoloma sublateritium (Fr.) Karst.
Kang, An-Seok ; Cha, Dong-Yeol ; Hong, In-Pyo ; Chang, Hyun-Yoo ; Yu, Seung-Hun ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 153~159
Effects of some sources on the vegetative growth of Naematoloma sublateritium (Fr.) Karst. were investigated using liquid and solid culture media. Temperature, pH, carbohydrates as carbon sources, amino acids as nitrogen sources, the optimal carbon/nitrogen ratio, mineral element and organic acids were studied for good mycelial growth. We could improve a new semisynthetic medium for mycelial growth in N. sublateritium.
Factors related to the growth of arbuscular mycorrhizal fungi in the plant roots
Lee, Sang-Sun ; Eom, Ahn-Heum ; Lee, Seok-Koo ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 160~171
Microscopic observations of arbuscular mycorrhizae (AM) were done with the colonizations of AM and the determinations of chitin in the plant roots of Sorghum bicolor, Cassia mimosoides, Capsicum annuum and Allium fistulosum. The intercellular and intracellular hyphae, arbuscules and vesicles were microscopically observed, according to increases of colonization of AM in the roots of four plants. The growth of AM fungi appeared sigmoid with the cultivation days after inoculation. The growth of AM fungi were inversely influenced by the additions of commercial fertilizers, P
A study on the production of arbuscular mycorrhizal fungal spores by using the commercial fertilizers and the pot culture techniques
Lee, Sang-Sun ; Eom, Ahn-Heum ; Lee, Seok-Koo ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 172~183
The productions of arbuscular mycorrhizal fungal(AMF) spores were observed by adding three different commercial fertilizers on AMF inhabiting soils. Various morphological features, vesicles, arbuscles, sporulations of spore, and flower-like-structures, were also found in the mycorrhizal roots during 80 days after transplanting. Spore prodcutions of the employed AMF were observed to be periodically increased with the intervals of 40 days. Sorghum, green onion, hot pepper, and wild legume plants were appeared to be a good plant for productions of AMF and as the host of AMF. The productions of AMF spores was inversely related to phosphate fertilizer, and also observed to be low in the plant pots added with whole balanced fertilizers.
Production of protein-bound polysaccharides by solid-state fermentation of Coriolus versicolor
Park, Kyung-Sook ; Park, Shin ; Jung, In-Chang ; Ha, Hyo-Cheol ; Kim, Seon-Hee ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 184~189
The possibility of solid-substrate fermentation of Coriolus versicolor for the production of protein-bound polysaccharides(PBP) was studied. Zeolite and orchid-pot soil were used as solid materials for the culture because of the desirable physical properties. Glucose, sucrose and starch showed to be good carbon sources for the production of PBP by the solid-substrate fermantation of C. versicolor. Among the nitrogen sources, bactosoyton and peptone were very effective for the PBP production. The optimum pH for solid-substrate culture for the production of PBP was at the range of 5-6. The yields of PBP reached to 5-6 mg per 100 g solid-substrate.
Effect of Sodium Hypochlorite(NaOCl) Treatment On Bacterial Yellow Blotch in Oyster Mushroom, Pleurotus ostreatus
Shin, Gwan-Chull ; Cho, Soo-Muk ; Jeon, Nak-Beom ; Ku, Ja-Hyeong ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 190~195
Studies were conducted to determine the potential of sodium hypochlorite(SHC) on the control of bacterial yellow blotch in cultivated oyster mushroom, Pleurotus ostreatus. SHC at the concentration of 80 ppm was effective on the control of Pseudomonas agarici causing yellow blotch in oyster mushroom except number 916 isolate. In vitro the mycelial growth was slightly inhibited at the concentration higher than 100 ppm of sodium hypochlorite, but retardation of the mycelial growth was soon recovered. Spray of SHC solution at the concentration of 40-50 ppm per day significantly reduced the incidence of the yellow blotch without impairing the growth of oyster mushroom in field culture. However, the higher concentration of SHC(67 ppm) induced yellow brown or dark gray in color and deformed cap and elongated stripe in morphology of fruiting body. Results indicate that periodical spray of sodium hypochlorite seems to be the recommendable method for protection against bacterial yellow blotch disease in oyster mushroom without reducing food quality.
Fungal flora of Ullung Island (V) -on additional agaric fungi-
Jung, Hack-Sung ;
The Korean Journal of Mycology, volume 22, issue 2, 1994, Pages 196~208
Some additional fungi were collected during two field trips to Ullung Island in July and September of 1992. Through the observation of agaric fungi, 36 mushrooms were identified to the species or subspecies and are listed below. Among them, three species and one subspecies, Mycena luteopallens, Mycena macrocystidiata, Amanita hemibapha ssp. similis, and Pluteus petasatus, were confirmed new to Korea and are registered here with descriptions.