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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 22, Issue 4 - Dec 1994
Volume 22, Issue 3 - Sep 1994
Volume 22, Issue 2 - Jun 1994
Volume 22, Issue 1 - Mar 1994
Selecting the target year
Growth Rate and Colony Morphology of Progenies of Zoospores and Oospores of Phytophthora cactorum causing Phytophthora rots in Apple Trees
Shim, Jae-Ouk ; Lee, Min-Woong ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 281~285
Zoospore progenies of Phytophthora cactorum were relatively uniform and similar to their respective parent in the rate of linear extension, whereas oospore progenies were greatly various. Also, the character of colony pattern was quantitatively various in oospore progenies but not zoospore progenies. Therefore, these results suggested that multiple genes were involved in determining growth rate and colony morphology of P. cactorum, and support the hypothesis that species of Phytophthora are diploid during the vegetative phase.
Production of Polygalacturonase from Ganoderma lucidum
Yoon, Sook ; Kim, Myung-Kon ; Hong, Jai-Sik ; Kim, Myeong-Sook ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 286~297
The optimum nutritional and cultural conditions of polygalacturonase by Ganoderma lucidum in liquid culture were studied. The optimal temperature, pH, and the duration of culture for production of the enzyme was
, 5.5 and 14 days, respectively. The maximal production of the enzyme was obtained in a synthetic medium containing 10 g of pectin, 10 g of soluble starch, 1 g of yeast extract, 2 g of peptone, 1 g of phenylalanine, 2 g of
, 0.2 g of
, 0.05 g of
and 100 g of
in 1000 ml of distilled water.
Purification and Properties of Polygalacturonase from Ganoderma lucidum
Yoon, Sook ; Kim, Myung-Kon ; Hong, Jai-Sik ; Kim, Myeong-Sook ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 298~308
The properties of polygalacturonase by Ganoderma lucidum in liquid culture were investigated. The enzyme was composed of an endo- and an exo-polygalacturonase. The endo- and exo-polygalacturonase were purified approximately 56 and 9.2-fold, respectively, through ammonium sulfate fractionation, gel filtration on Biogel P-100, anion exchange chromatography on DEAE-cellulose, gel chromatography on Sephadex G-150 and re-gel chromatography on Sephadex G-150. The endo- and exo-polygalacturonase had higher affinity for apple pectin than for citrus pectin or pectic acid. The Km values of the endo- and exo-polygalacturonase for apple pectin, determined on the Lineweaver-Burk plot, were 1.44 and 10.6 mg
for apple pectin, respectively. Purified endo-polygalacturonase was found to be homogeneous electrophoretically and had a molecular weight of 54,000 estimated on SDS polyacrylamide gel. The optimal pH for the activity of the enzymes was 4.0. The endo- and exo-polygalacturonase were stable in the pH range of 4.0 to 6.0 and 3.5 to 5.5, respectively. The optimal temperatures of the endo- and exo-polygalacturonase were 40 and
, respectively. The exo-polygalacturonase was more resistant to heat than the endo-polygalacturonase, requiring heating for 40 min at
for complete inactivation. The activity of the endo-polygalacturonase was increased by
, while that of the exo-polygalacturonase was increased by
only, and was not affected by
Anastomosis Groups and Cultural Characteristics of Rhizoctonia solani Isolates from Crops in Korea
Kim, Wan-Gyu ; Cho, Won-Dae ; Lee, Young-Hee ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 309~324
A total of 2,276 isolates of Rhizoctonia solani obtained from diseased crops of 68 species was classified into anastomosis groups AG-1, AG-2-1, AG-2-2, AG-3, AG-4 and AG-5 by anastomosis test. Among the isolates, 1,091 isolates were identified as AG-1, 326 isolates as AG-2-1, 191 isolates as AG-2-2, 71 isolates as AG-3, 505 isolates as AG-4, and 92 isolates as AG-5. Among the isolates of AG-1, 791 isolates were grouped as cultural type IA, 280 isolates as cultural type IB, and the others as cultural type IC. Among the isolates of AG-2-2, 112 isolates were grouped as cultural type IIIB, and the others as cultural type IV. Cultural types IA, IB and IC of AG-1 were isolated from 7, 26 and 2 species of crops, respectively. AG-2-1 was isolated from 10 species of crops. Cultural types IIIB and IV of AG-2-2 were isolated from 7 and 3 species of crops, respectively. AG-3 was only isolated from Solanum tuberosum. AG-4 was isolated from 43 species of crops, and AG-5 from 13 species of crops. A single anastomosis group was isolated from each of 45 species of crops, but two or more than two anastomosis groups were isolated from each of the other crops. Cultural appearance of the isolates belonging to an anastomosis group or a cultural type was mostly distinct from that belonging to others, although cultural appearances of some anastomosis groups or cultural types were similar to one another. Optimum temperature for mycelial growth of AG-1, AG-2-2, AG-4 and AG-5 ranged from 26 to
, and that of AG-2-1 and AG-3 from 22 to
. Minimum temperature for mycelial growth of AG-2-1 was the lowest as
, that of AG-1(IA) and AG-4 was the highest as
, and that of the others ranged from 5 to
. Maximum temperature for mycelial growth of AG-2-2(IIIB) was the highest as
, that of AG-2-1 was the lowest as
, and that of the others ranged from 31 to
. When the mycelial growth rates at
were compared, AG-1(IC) grew most rapidly, followed by AG-1(IA) and AG-1(IB), and AG-2-1 grew most slowly.
Correlative Production of Mn-Peroxidase and Glucose Oxidase Depending on the Culture Condition of Schizopora paradoxa
Lee, Sang-Yoon ; Shin, Hyeon-Dong ; Kim, Kyu-Joong ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 325~331
White-rot fungus, Schizopora paradoxa did not produce Mn-peroxidase and glucose oxidase without manganese. But, in high concentration of manganese (40 ppm), the activities of both enzymes were higher than those in basal concentration of manganese (11.15 ppm). Unlike the activities of the enzymes, mycelial mass was the same level as the control culture (11.15 ppm manganese) through out the culture period, depending on the concentration of manganese. The same experiments were carried out for the effect of copper and veratryl alcohol added to the culture. The results were not consistent dependent on the concentration of copper and veratryl alcohol, respectively. The involvement of cAMP in the correlative production of MNP and GOX was investigated. In this study, addition of atropine to the culture resulted in a concomitant inhibition of production of MNP and GOX, depending on the concentration of inhibitor added.
Effect of Various Carbon Sources on the Development of Aspergillus nidulans with
or velA1 allele
Han, Dong-Min ; Han, Yoo-Jeong ; Chae, Keon-Sang ; Jahng, Kwang-Yeop ; Lee, Young-Hoon ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 332~337
Under standard condition (Han, et al., 1990: glucose 1%-nitrate 0.1% minimal medium, 30 ml in 9 cm plate,
cells of inoculum per plate), wild type of Aspergillus nidulans developed both sexual and asexual organs in ballance, while velA1 mutant developed asexual ones preferentially. Increase of glucose concentration did not significantly affect the asexual sporulation. However, development of sexual organs were largely affected. It was greatly enhanced when favorable nitrogen source, for example, casein hydrolysate was added, which is contrary to the case of Neurospora or Saccharomyces where limitation of N source induces sexual development. On most of moderate C sources asexual development in
strain was largely inhibited except acetate on which only asexual spores were produced, while that in velA1 mutant strain was not affected. Lactose promoted the sexual development even in velA1 mutant indicating that lactose itself or its metabolic intermediate may induce sexual development independent of allelic state of velA gene. On other moderate favorable C sources, glycerol, galactose and ethanol, asexual development was largely inhibited in
strain but not in velA1 mutant strain. Sexual organs were, however, never produced on acetate. These results suggested that asexual development of wild type is largely dependent on C sources and the velA gene is involved in the repression of asexual development in not-enough-grown (non-competent) thalli resulting in preferential progression of sexual development.
Studies on the Changes of Amino acid Contents on Pleurotus ostreatus
Ryu, Jeong ; Lee, Gong-Joon ; Jung, Gi-Tai ; Na, Jong-Seong ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 338~342
Amino acids of Pleurotus ostreatus in various cultural media(rice straw, sawdust and cotton waste) were analyzed to recognize the compositional differences depending on pileus size and portions(pileus and stipe) Total amino acids of rice straw were a little less than that of sawdust and cotton waste. Seventeen amino acids were identified and quantified. Among the amino acids glutamic acid was higest and cystine was lowest. The amino acid contents depending on the pileus size was higest in
of rice straw, under 3 cm of sawdust, over 7 cm of cotton waste, respectively. The content of them was found to be higher in pileus than stipe.
Vegetative Incompatibility and Hypovirulence Conversion of Cryphonectria(Endothia) parasitica(Murr.)Barr
Lee, Jong-Kyu ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 343~349
One hundred and two virulent(V) strains of Cryphonectria parasitica were isolated from the cankers of American chestnut (Castanea dentata) trees in western Massachusetts, USA. The diversity of vegetative compatibility groups (VCGs) of C. parasitica was investigated. One hundred and two strains represented 54 VCGs; 38 VCGs had only one strain each, 6 VCGs had 2 strains each, and 10 most common VCGs had 52 strains. Great diversity in VCGs may due to the increasing numbers of VCGs with time since the pathogen has been in Massachusetts for 80 years. Ten vegetative compatibility representative strains were selected from the 10 most common VCGs and converted to hypovirulent (H) strains through the pairing and hyphal anastomosis of H strains (4 strains with French dsRNA elements and 17 strains with Italian dsRNA elements). All of the 10 representative strains were converted to H strains by at least more than one of the H strains.
Selection of Highly Bitter taste Strains on Ganoderma sp.
Kim, Kyung-Soo ; Byun, Myung-Ok ; You, Chang-Hyun ; Cha, Dong-Yeul ; Jeong, Hoon ; Ko, Mi-Suk ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 350~354
A study was conducted on the characteristics of 11 strains of Ganoderma sp. to select the strains with more bitterness. Among the flat type strains, ASI 7071 and 7091 showed higher bitterness, while among the branched type strains ASI 7074 and 7094 were found to more bitter than other strains. The growth of ASI 7091 was best on Ganoderma complete media(GCM), while ASI 7010, 7048 and 7075 performed best in oak saw dust media. Among the branched type strains the esterase isozyme band patterns were similar. On the other hand among the flat type strains, the esterase isozyme band patterns differed from each other. Out of 11 strains, ASI 7004 was found to have the heaviest fruiting body, ASI 7071 the thickest cap and ASI 7094 the biggest cap.
Isolation and Identification of Hyperparasites against Powdery Mildew Fungi in Korea
Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 355~365
An extensive ssurvey was conducted on the occurrence of hyperparasites (HP) on powdery milew species in Korea during
seasons. As a result, a total of 1070 materials infected with powdery mildew fungi were collected. Of these, 92 ones were infected with the HP; 6 with the unidentified HP and the rest 86 with Ampelomyces quisqualis. This showed infection of powdery mildew species with HP was common phenomenon in nature and A. quisqualis was the most common HP in Korea. To prove the hyperparasitism of A. quisqualis, 24 isolates from 32 collections in 1992 year were successfully cultured. All isolates tested were hyperparasitic to cucumber powdery mildew, Sphaerotheca fusca.
The Effect of Antibiotics on the DNA Synthesis and Base Composition in Fungal Cells
Park, Kyou-Yeon ; Lee, Chong-Sam ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 366~377
The base composition of DNA of Aspergillus phoenicis, Rhizopus acidus and Candida albicans treated with cycloheximide and nalidixic acid during the culture was analyzed to compare with the control. The contents of base in the DNA were inhibited by cycloheximide, 20.4% of adenine, 43.1% of thymine, 40.9% of cytosine, 35.3% of guanine, 32.2% of purine, and 42.7% of pyrimidine for A. phoenicis. In R. acidus, 34.2% of adenine, 42.1% of thymine, 38.0% of cytosine, 18.1% of guanine, 24.1% of purine and 40.0% of pyrimidine were depressed by cycloheximide. In the antibiotic treatment of C. albicans, 58.3% of adenine, 58.5% of thymine, 58.1% of cytosine, 42.4% of guanine, 46.8% of purine and 58.8% of pyrimidine were inhibited to compare with the control. The nalidixic acid treatments were showed that, in A. phoenicis 41.6% of adenine, 47.1% of thymine, 59.3% of cytosine, 46.3% of guanine, 45.6% of purine and 57.2% of pyrimidine were inhibited. When R. acidus was treated with nalidixic acid, 59.1% of adenine, 54.7% of thymine, 35.3% of cytosine, 37.4% of guanine, 45.9% of purine and 44.9% of pyrimidine decreased. In treatment of nalidixic acid, the content of DNA was depressed 60.1% of adenine, 68.6% of thymine, 60.7% of cytosine, 40.0% of guanine, 45.8% of purine and 63.5% of pyrimidine for C. albicans In the DNA synthesis of three fungal cells, cycloheximide and nalidixic acid treatments were analyzed obviously that the biosynthesis of pyrimidine was depressed than that of purine. Therefore, it was showed that the DNA contents in the various fungal cells were inhibited remarkably in nalidixic acid treatment than cycloheximide.
Interspecific Hybridization between Pleurotus ostreatus and Pleurotus sajor-caju by Protoplast Fusion
Yoo, Young-Bok ; Lee, Haing-Sook ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 378~385
Interspecific somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus and Pleurotus sajor-caju. The fusion products between incompatible strains did not form clamp connections. Fruiting body of the clampless fusants was induced by light-dark cycle on saw-dust-rice bran substrate in glass bottles. Out of them, seven somatic hybrids produced fruiting bodies of intermediate morphology of the two species. Light and low temperature were the initiating factors for the development of clamped hyphae from the clampless mycelial colonies. All of these basidiocarps had clamp connections. Eight fusants from the six crosses were analysed with the segregation of genetic characters by random spore isolates. In the three combinations, unexpected alleles were shown. Somatic hybrid between P188 (P. ostreatus 2-1 + P. sajor-caju 2-53) and P. florida 2-3 by triple cross produced fruiting bodies similar to those of fusant between P. ostreatus and P. florida. All the genetic charaters from the three strains were shown to segregate and recombine.
Isozyme patterns of section Elegans, section Liseola and similar species in the genus Fusarium
Min, Byung-Re ; Kweon, O-Yeong ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 386~393
To investigate taxonomical relationships of Fusarium species,
, acid phosphatase, malate dehydrogenase, peroxidase and polygalacturonase were extracted and the isozyme patterns were compared by using polyacrylamide gel electrophoretic analysis. Only polygalacturonase was monozyme and the other enzymes showed little differences in banding patterns. Genetic similarities based on the isozyme banding patterns were as follows: the interspecific similarity between F. subglutinans and F. moniliforme in Liseola showed the closest relationship of 74.3% of all species studies. And the similarity between section Elegans and section Liseola was 45.4%. F. napiforme and F. nygamai showed the similarity of 64.7%, similar to the correlation between species in the same section. The similarity of these two species to Liseola and Elegans showed 55.2% and 45.4%, being revealed that they would be closer to Liseola than Elegans. However, these results were similar to those of any other sections. Therefore it suggested that these 2 species should be in a different section from any other sections. And F. graminearum showed the similarity of 28.2% to the other 6 species.
Ecological Study on Arbuscular Mycorrhizae(AM) at Coastal Reclaimed Lands
Koh, Sung-Duk ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 394~409
The symbiotic activities of arbuscular mycorrhizal fungi(AMF) such as spore density, symbiotic intensity and vesicle density, phytomasses of higher plants such as Calamagrostis epigeios, Imperata cylindria, Artemisia scoparia, Aster tripolium and Sonchus brachyotus and seasonal change of the AMF activities, electric conductivity and zinc contents in plant and soil were determined in the rhizospheres of higher plants at abandoned old coastal reclaimed lands, where constructed in 12 and 30 years ago. If plants of reclaimed land classified to salinity, symbiotic activities of AMF were high in order of obligate halophyte, facultative halophyte and glycophyte. Also, those plants classified to life form, symbiotic activities of AMF were high in order of annual, biennial and perennial plants. Seasonal variation of spore density, one of symbiotic activities showed that the plateau density maintained continuously from the end of growing season of the higher plants to next spring. For this reason, it regarded that reproduction of AMF spore would be formed in autumn, when the higher plants will be developed. Seasonal change of symbiosis intensity, other symbiotic activities, however, showed that the highest symbiosis intensity occurred in spring and summer but the lowest in autumn. In relationships among symbiotic activities, spore density was directry proportional increase of symbiosis intensity. Moreover, phytomass of higher plants also was directly proportional to increase the spore density as well as symbiosis intensity. Vesicle density, however, did not any correlation with the phytomass, spore density and symbiosis intensity. From these results, it can know that both spore density and symbiosis intensity are strongly possible to use as the measure of symbiotic activity owing to symbiosis of tho-AMF, the more absorption of zinc by the higher plants carried out the less concentration of zinc in the soil.
Fungal Flora of Mt. Chiak (1) -Agaric Fungi-
Kim, Yang-Sup ; Seok, Soon-Ja ; Park, Yong-Hwan ; Cha, Dong-Yeol ; Min, Kyong-Hee ; Yoo, Kwan-Hee ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 410~420
Some agaric fungi were collected at Chink from Sep. 10, 1988 and Aug. 12, 1990. some agaric fungi were recognized to the species. Among them six species were confirmed new to Korea and registered here with descriptions. They are Calocybe ionides (Bull.: Fr.) Donk ; Tricholoma psammopus (Kalchbr.) Quel.; Collybia cirrhatta (Per.) Quel.; Marasmius aurantioferrugineus Hongo; Amanita abrupta Peck and Bull.; Amanita castanopsidis Hongo.
Notes on Korean Agaricales (III)
Seok, Soon-Ja ; Kim, Yang-Sup ; Park, Dong-Suk ;
The Korean Journal of Mycology, volume 22, issue 4, 1994, Pages 421~426
Some Agaric fungi were collected from the area of Suwon, Mt. Taewha and Mt. Yongmoon in Kyonggi-do and Chongpyong temple in Kangwon-do. Among them, one genus and five taxa of Agaricales were confirmed new to Korea. In this paper we registered five texa of Agricales in detailed description such as Hygrocybe firma (Berk. & Br.) Sing. var. firma Sing.; Hohenbuehelia petalodes (Bull.: Fr.) Schulzer; Cystoagaricus strobilomyces (Mull.) Sing.; Coprinus domesticus (Bolt.: Fr.) S.F.Gray and Inocybe acutata T. Koba.& E.Naga.. The one genus new to Korea is Cystoagaricus Sing.