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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 23, Issue 4 - Dec 1995
Volume 23, Issue 3 - Sep 1995
Volume 23, Issue 2 - Jun 1995
Volume 23, Issue 1 - Mar 1995
Selecting the target year
Solid-culture of Lepista nuda
Lee, Sang-Sun ; Choi, Kyoung-Jin ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 105~113
The mycelia of Lepista nuda were isolated from the different basidiocarps, collected from the forests of Robinia pseudo-acaciae and mixed forests of R. pseudo-acaciae and Quercus serrata. On the different woods, the growths of mycelia collected were compared with those transferred from Applied Mycology Division, RDA. Also, the measurements of dry weight loss, as growth of mycelia, were correlated to the productions of carbon dioxide on the different wood substrates. The growth of mycelia was observed to be best on the woods of R. pseudo-acaciae; followed by the growth on the woods of Morus alba. The growth rate of mycelia appeared to be various, depending on the different kinds of wood substrates. The soild-cultures of the mycelia on the sawdust of the R. pseudo-acaciae woods and the grains of sorghum and corn were conducted, but the basidiocarps were not obtained from these. Therefore, this mycelium collected was believed to be important as new mushroom utilizing the different woods resources instead of Q. serrata.
Seed-borne Infection of Anthracnose Fungi Isolated from Diseased Red Pepper
Lee, Du-Hyung ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 114~120
Colletotrichum dematium, C. gloeosporioides and Glomerella cingulata were detected in seed samples collected from diseased red pepper (Capsicum annuum) using blotter method. C. gloeosporioides was the predominant species in seed samples tested and followed by C. dematium and G. cingulata. When the seed components were plated C. dematium, C. gloeosporioides and G. cingulata were detected from seed coat, endosperm and cotyledon. The three anthracnose fungi were recorded more frequently from seed coat than that of observed in the endosperm and cotyledon. Seed infection with C. dematium, C. gloeosporioides and G. cingulata caused seed rotting, damping off and seedling blight of red pepper plants. According to the inoculation experiments, it was shown that C. gloeosporioides was the most virulent among three species. C. dematium showed weak virulence when the plants were wounded, and G. cingulata was wound parasite or weakly virulent on red fruits. Benlate T (benomyl+thiram) and Homai (thiophnate-methyl+thiram) were effective to anthracnose fungi when treated to infected seeds.
Isolation and purification of protein-bound polysaccharides from the sawdust mycelia of Agrocybe cylindracea
Ha, Hyo-Cheol ; Park, Shin ; Park, Kyung-Sook ; Lee, Chun-Woo ; Jung, In-Chang ; Kim, Seon-Hee ; Kwon, Yong-Il ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 121~128
The characteristics of protein-bound polysaccharides (PBP) which were isolated and purified from the sawdust mycelia of Agrocybe cylindracea were investigated. The yield of crude protein-bound polysaccharides (Fr.CB) extracted with boiling water and precipitated with 95% ethanol, was 0.74% based on the original sawdust mycelia. The Fr.CB was purified by the membrane filtration, ion exchange chromatography and gel filtration. The Fr.B fraction of which the molecular weight is over 300 KDa, was isolated from the Fr.CB using membrane filtration, and the yield was 38.6% based on the Fr.CB. This result indicates that high molecular protein-bound polysaccharides are the dominent components of the Fr.CB. Two fractions (Fr.B-1, Fr.B-2) were also isolated from the Fr.B using ion exchange chromatography, and the yields were 17.3% (Fr.B-1) and 10.3% (Fr. B-2), respectively. The Fr.B-1 was concentrated and gel-filtrated, and the single peak, thought to be nearly pure protein-bound polysaccharides, was obtained. The yield of final fraction
was 42.5% based on the Fr.B-1. The molecular weight of
was nearly 710 KDa. The monosaccharides' composition of
was analized by HPLC, and glucose was the dominent component, and fucose and galactose were also detected. The result of amino acid analysis was that glutamic acid and analysis were detected to a significant level, and cysteine was not detected.
Cloning of a Gene Involved in Biosynthesis of
in Saccharomyces cerevisiae
Jin, Eun-Hee ; Lee, Dong-Won ; Kim, Jin-Mi ; Park, Hee-Moon ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 129~138
DNA fragment being able to restore in vitro activity of
synthase was cloned by transformation of the Saccharomyces cerevisiae LP353 mutant strain with genomic library constructed in the YCp50. For the selection of transformants which showed no detectable phenotype linked to recovery of the defect in
synthase activity, the colony autoradiography was succesfully applied. The restriction map of the cloned DNA fragment, which is 8.5-kb in length, was constructed. Both the YEplac195 and the YCp50 carrying the 8.5-kb fragment increased
synthase activity of LP353 by two fold. Neither the YEplac195 nor the YCp50 carrying the 8.5-kb DNA fragment, however, complemented the temperature-dependent osmotic sensitivity which is another distinctive phenotype of LP353. Subcloning experiments indicated that a functional region was located in 4.8-kb BglII-KpnI fragment. The 4.8-kb fragment was also able to increase the level of
content in cell wall as well as the resistance of cells to cell wall lytic enzyme,
. The growth rate of the LP353 with 4.8-kb fragment was almost same as that of wild type strain in liquid medium with 1.2 M sorbitol at nonpermissive temperature. Taken these results together, the 4.8-kb fragment seemed to contain the BGS2 gene for
synthase activity in yeast S. cerevisiae.
Cladosporium alliicola sp. nov. on Allium victorialis var. platyphyllum
Shin, Hyeon-Dong ; Braun, Uwe ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 139~143
A species of Cladosporium isolated from Allium victorialis var. platyphyllum Makino differs from C. allii and C. allii-cepae by catenate, multiseptate conidia and aseptate conidiophores. Comparable Cladosporium spp. on other substrates are unknown. Therefore, this fungus must be considered as a new, undescribed species, viz. Cladosporium alliicola sp. nov.
Higher Fungi in Korea (1)
Seok, Soon-Ja ; Kim, Yang-Sup ; Ryu, Young-Jin ; Park, Dong-Suk ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 144~152
Through a floral study and resource investigation of Korean mushrooms during the year 1993, five species of Agaricales, Pseudoclitocybe cyathiformis (Bull.: Fr.) Sing.; Pholiota brunnescens A.H. Smith & K. Hesler; Coprinus angulatus Peck; Rhodophyllus bisporus Hongo; Suillus viscidipes Hongo and one form of Gasteromycetes, Lysurus mokusin (L.: Pers.) Fr.f. sinensis (Lloyd) Kobayashi, were found and described new to Korean flora. Pseudoclitocybe is described as an unrecorded genus to Korea. The color names cited are from Kornerup & Wanscher's Methuen Handbook of Colour (1984). All the specimens are deposited in the RDAGB's and ASIK's herbarium.
Antimicrobial Activity of Elfvingia applanata Extract Alone and in Combination with Naringenin
Cheon, Jun-Hee ; Lee, Sun-Ok ; Rym, Kyo-Hwan ; Eo, Seong-Kug ; Lee, Chong-Kil ; Han, Seong-Sun ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 153~160
As part of our search for less toxic antimicrobial agents from natural resources, the antimicrobial activity of Elfvingia applanata
extract was examined alone and in combination with naringenin. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against nineteen strains of bacteria and eleven strains of fungi by serial broth dilution method, and expressed by minimal inhibitory concentration (MIC). Among nineteen strains of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.125 mg/ml. EA also inhibited the growth of the selected fungi at higher concentrations ranging from 7.5 mg/ml to 15.0 mg/ml. To investigate the effect of antimicrobial combinations of EA with naringenin, the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with naringenin resulted in partial synergism against Staphylococcus aureus only, and showed additive effect in two strains including Klebsiella pneumoniae and Salmonella typhi. Antagonism was not found.
Meju Fermentation for a Raw Material of Korean Traditional Soy Products
Lee, Sang-Sun ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 161~175
Meju is a raw material used to make Korean soy sauce (Kanjang) and soybean paste (Doenjang), both of them rich in plant protein. The twenty-nine fungal and a bacterial species were identified from twenty-three traditionally homemade meju cakes. Out of them, only a few species were found to be involved in the actual fermentation process of meju; The other species were contaminants during the improper subprocesses of meju fermentation. The fungal floral successions were observed to be related to two physical and biochemical changes of meju cakes during meju fermentation: drying and heat releasing processes. The zygomycetous fungi were first observed to exist mainly during the first stage. Scopulariopsis brevicaulis was observed to grow on the surface of meju cakes and then to coexist with Bacillus megatrium in the inner part of meju cakes during the second stage. Based on the biochemical tests, the proteases secreted by the different microorganisms were involved in the degradation of soybean proteins with a mutual relationship. Also, zygomycetous fungi were speculated to be important microorganisms for inducing the second stage in the traditional Korean homemade meju.
Study on the Screening and Development of Antibiotics in the Mushrooms -The Screening of Bacterial and Fungal Antibiotics in Basidiomycetes (II)-
Park, Sang-Shin ; Lee, Kap-Duk ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 176~189
For the development of antibiotics from Korean mushrooms, the biological activities of extracts from 98 species of mushrooms and from 25 mushrooms were tested against 9 different Gram-negative bacteria and 8 fungi, respectively. Fruiting bodies of each mushrooms were extracted with petroleum ether (P), 80% ethanol (E), and distilled water (H) in that order. P, E, or H extracts from 20 mushroom samples exhibited the antibacterial activity against 8 different Gram-negative bacteria containing Klebsiella pneumoniae, selectively. Among the mushroom extracts with antibiotic activity, E extracts of Boletus umbriniporus, Armillariella tabescens, Rhodophyllus sinuatus, and Suillus luteus showed various antibiotic activities against several bacteria. E extracts of Abortiporus biennis, Phellinus gilvus, and Polyporus dispansus are highly active against Salmonella typhi and their minimum inhibitory concentration (MIC) was all
. E extract of Armillariella tabescens showed the antifungal activity against Trichopyton mentagrophytes, and its MIC was
Screening for Phyllospheral Antagonistic Microorganisms for Control of Red-pepper Anthracnose (Collectotrichum gloeosporioides)
Paik, Su-Bong ; Kim, Dong-Woo ;
The Korean Journal of Mycology, volume 23, issue 2, 1995, Pages 190~195
For the screening of biocontrol agents against red-pepper anthracnose (Colletotrichum gloeosporioides Penz) 248 isolates of bacteria, 51 of fungi and 30 of yeasts were obtained from phyllospere of medicinal plants. Of isolated microorganisms, four bacterial isolates, KB6, KB12, KB13 and KB14 were highly antagonistic to C. gloeosporioides than the others through dual culture test on potato dextrose agar (PDA). Among the four bacterial isolates, culture filtrate of the isolate KB12 showed the highest inhibition of C. gloeosporioides on PDA. The culture filtrates of four isolates controlled anthracnose on the red fruits, but not on the green fruits. In the living bacterial cell test, high control effect was observed both on the red and the green fruits. In the biochemical test, all isolates were identified as Bacillus subtilis.