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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 23, Issue 4 - Dec 1995
Volume 23, Issue 3 - Sep 1995
Volume 23, Issue 2 - Jun 1995
Volume 23, Issue 1 - Mar 1995
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The Composition and Bioactivities of Ganoderan by Mycelial Fractionation of Ganoderma lucidum IY009
Han, Man-Deuk ; Jeong, Hoon ; Lee, June-Woo ; Back, Sung-Jin ; Kim, Su-Ung ; Yoon, Kyung-Ha ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 285~297
Ganoderan, an immunomodulating
of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. The present study was set up to elucidate the chemical composition and bioactivities of ganoderan obtained from the mycelial fractionation of G. lucidum IY009. Ganoderan was isolated and purified from its extracellular, cell wall and cytoplasmic sources. These ganoderans were composed mainly of glucose. The cell wall-alkali soluble-water soluble fraction (CW-AS-WS) showed the highest antitumor activity (inhibition rate of 94%) in sarcoma-bearing mice and 37% of anticomplementary activity. The CW-AS-WS fraction was found to be approximately average 20,000 dalton in aq. 0.3N NaOH solution and composed of 88% carbohydrate and 4% protein. The carbohydrate of the CW-AS-WS was composed of 74% glucose. These results indicate that the ganoderans extracted from the mycelial fractionations of G. lucidum IY009 had different chemical characteristics and showed different potentiality in antitumor and anticomplementary activity.
Decolorization of Dyes by White Rot Fungi
Kim, Hyoun-Young ; Leem, Young-Eun ; Choi, Hyoung-Tae ; Song, Hong-Gyu ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 298~304
Decolorization of poly R-478, congo red and methylene blue by 5 white rot fungi which were isolated in Korea has been carried out. Coriolus versicolor KR-11W and C. versicolor KR-65W gave the best results when they were grown under stationary culture. C. versicolor KR-11W decolorizes 100% of poly R-478 in 13 days, 100% of congo red in 7 days and 90% of methylene blue in 7 days. C. versicolor KR-65W decolorizes 100% of poly R-478 in 15 days, 85% of congo red in 7 days and 100% of methylene blue in 7 days. Phanerochaete chrysosporium IFO 31249, which was used as a control, decolorizes 35% of poly R-478 in 15 days, 85% of congo red in 7 days and 95% of methylene blue in 7 days.
Protoplast Formation and Regeneration of the Wood-Rot Basidiomycete Phanerochaete chrysosporium
Jun, Sang-Cheol ; Kim, Kyu-Joong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 305~309
To investigate optimal conditions for the protoplast formation and regeneration of Phanerochaete chrysosporium, preparations of three enzymes were used to liberate protoplasts from its 20 hrs-old mycelium on cellophan membrane covered agar media. Novozym 234 alone with 0.6M sucrose was the most effective for isolation of protoplasts from the mycelium with 3hrs incubation time at
in shaking condition of 120 rpm. The poly-R medium stabilized with 0.6M mannitol was the best for regeneration of the protoplasts.
Phylogenetic Relationships of Yeast-like Fungi Deduced from Partial Sequences of 18S Ribosomal RNA
Jung, Soo-Jin ; Shin, Yong-Kook ; Joo, Woo-Hong ; Lee, Jae-Dong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 310~317
The evolutionary relationships for 43 species in the taxa of the basidiomycetous yeast and related yeast-like fungi have been studied from the 43 18S rRNA partial sequences. Fibulobasidium inconspicuum showed a relatively close relationship to Filobasidiella neoformans. It is obvious that Tremella foliacea and Ustilogo rabenhorstiana form a independently different cluster from basidiomycetous yeast and seem to be phylogenetically distant from basidiomycetous yeast and each other. The basidiomycetous yeasts are believed more closely associated with Tremella foliacea than with Ustilago rabenhorstiana. The phenotypic criteria such as carotenoid pigments, teliospores, ballistospores seems not to be phylogenetically useful indicators.
Evolutionary Relationships of the Genus Trichoderma and Related Taxa Based on the Partial Sequences of 18S Ribosomal RNA
Lee, Goang-Jae ; An, Won-Gun ; Lee, Jae-Dong ; Joo, Woo-Hong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 318~324
The evolutionary relationships of the genus Trichoderma and related taxa were assessed using partial sequencing of 18S ribosomal RNA. Phylogenetic tree divided into three major groups; 1. Saccharomyces cerevisiae-Geotrichum klebahnii-Alternaria mali group; 2. Neurospora crassa-Aspergillus-Penicillium-Chrysosporium pannorum-Scopulariopsis sp. group; 3. Trichoderma group. The genus Trichoderma seemed to be phylogenetically separated from Saccharomyces cerevisiae, Aspergillus and Penicillium groups, and have passed through it's own evolutionary pathway.
Effect of Cultural Conditions on Polysaccharide Production and its Monosaccharide Composition in Phellinus linteus L13202
Lee, Jae-Hoon ; Cho, Soo-Muk ; Ko, Kyung-Soo ; Yoo, Ick-Dong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 325~331
The effect of cultural conditions on mycelial growth, polysaccharide production in Phellinus linteus and its monosaccharide composition was studied. P. linteus showed the highest growth (0.9 g/100 ml) on glucose but the polysaccharide production was the highest (13.7%) on mannose. The fungus grew very well at neutral pH (0.9 g/100 ml) but the growth was reduced to 0.47 g per 100 ml at alkaline pH. For the different pH, the yield of polysaccharide was in the range of
. The highest yield of 7.94% was obtained at pH 5. Also a variation in monosaccharide composition was observed for different carbon sources and pH. The composition ranges of glucose, mannose, and galactose of polysaccharide were
depending on carbon sources, respectively. In contrast, the variation of composition range of three monosaccharides was narrower for different pH than that for carbon sources. These results suggested the possibility of the improvement of production and the physiological modification of the polysaccharide.
Immuno-stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (I) - Characterization of polysaccharides extracted with neutral sodium chloride solution -
Cho, Soo-Muk ; Lee, Jae-Hoon ; Han, Sang-Bae ; Kim, Hwan-Mook ; Yu, Seung-Hun ; Yoo, Ick-Dong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 332~339
Water-soluble polysaccharide (FCW) was extracted from the fruiting body of Fomitella fraxinea with neutral sodium chloride solution. The polysaccharide was further fractionated into FCW-I and FCW-II by ion exchange chromatography. The FCW-I and FCW-II were then purified by gel permeation chromatography and named as FCW-Ia and FCW-IIa, respectively. FCW-IIa showed relatively strong immuno-stimulating activity but FCW-Ia did not. By analyses of HPLC and GPC, FCW-Ia and FCW-IIa were identified to be homogeneous and their molecular weights were estimated to be about 15,000 and 8,700, respectively. FCW-Ia consisted of fucose, galactose, and mannose as main sugars and their molar ratio was 19.5 : 63.2 : 25.0. Protein was not detected in FCW-Ia. However, FCW-IIa was composed of glucose, galactose, and mannose at a molar ratio of 1.0 : 0.3 : 0.4 and contained 0.4% protein with a higher amount of glutamic acid. A small amount of uronic acid was detected in both FCW-Ia and FCW-IIa.
Immuno-stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (II) -Isolation and characterization of hot-water extracted polysaccharides-
Cho, Soo-Muk ; Lee, Jae-Hoon ; Han, Sang-Bae ; Kim, Hwan-Mook ; Yu, Seung-Hun ; Yoo, Ick-Dong ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 340~347
Polysaccharide FHW was extracted from the fruiting bodies of Fomitella fraxinea with hot-water treatment and then fractionated into FHW-I and FHW-II on DEAE-Cellulose chromatography. FHW-I and FCW-II were further purified into FHW-Ia and Ib, FHW-IIa and IIb on gel permeation chromatography, respectively. A small amount of uronic acid was detected and glucose, galactose, fucose, and mannose were found to be main sugars in the polysaccharides. Protein was detected in FHW-Ia, FHW-IIa, and FHW-IIb, but not in FHW-Ib. FHW-Ia was identified to be a fuco-gluco-mannogalactan with molecular weight of 19,000 and FHW-Ib was a gluco-fuco-mannogalactan of 15,000. FHW-IIa and FHW-IIb were galacto-mannoglucan and their molecular weights were estimated to be 31,000 and 9,000, respectively. Both FHW-Ib and FHW-IIb did not show an absorption band characteristic of the
linkage in IR spectra. FHW-IIb showed a strong immuno-stimulating activity but the other three polysaccharides showed a weak activity.
Biodegradation of Poly (3-hydroxybutyrate) by Penicillium pinophilum
Kim, Mal-Nam ; Kang, Eun-Jung ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 348~353
Biodegradability of poly (3-hydroxybutyrate) (PHB) by Penicillium pinophilum was investigated by the modified Sturm Test. The biodegradability measurement by this method was more reproducible than other conventional activated sludge methods. Optimum inoculum size for the PHB biodegradation was 1% (v/v). The degradation appeared to occur not only on the sample surface but also inside the sample because the biodegradation did not increase quite proportionally with the sample surface area. The biodegradation rate increased to an asymptotic value as the nitrogen content in the test medium increased, indicating the nitrogen source was needed for the synthesis of the PHB depolymerase.
Yeast Loading BOD Biosensor
Kim, Mal-Nam ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 354~358
A yeast loading biochemical oxygen demand (BOD) sensor was designed and constructed to quickly measure the concentration of biologically assimilable organic substances dissolved in water as BOD values to feed back to the waste water treating processes. The sensitivity of the BOD sensor reached maximum at around pH 7.0 and
where yeast showed the highest assimilation activity. Biomass also affected the sensor output, and biomass of
on the dialysis membrane appeared to be the optimum cell mass level. The sensitivity of the sensor depended on the kinds of pollutants and increased considerably when the yeast was preincubated in the solution of respective pollutants before loading on the sensor.
Comparision of Polypeptide Patterns by 2-D PAGE in Fusarium Species
Min, Byung-Re ;
The Korean Journal of Mycology, volume 23, issue 4, 1995, Pages 359~368
F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.