Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 1996
Volume 24, Issue 3 - Sep 1996
Volume 24, Issue 2 - Jun 1996
Volume 24, Issue 1 - Mar 1996
Selecting the target year
Effects of the Quantities of Substrate on the Yield of Oyster mushroom Pleurotus ostreatus
Yun, Young-Seok ; Rew, Young-Hyun ; Park, Sun-Do ; Choi, Boo-Sull ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 89~92
This study were carried out to evaluate the effect of quantities of substrate on the yields for 2 years
. The substrates are cotton waste, rice straw and rice hull. It were revealed that cotton waste mixed rice straw was the best yield of fruit body. It gave more increased
than other substrates
when increasing the quantities of substrate. And cotton waste mixed with rice hull was most fast for first pinhead formation.
Effect of a Soil Amendment for Controlling Fusarium Wilt of Cucumber caused by Fusarium oxysporum f. sp. cucumerinum
Chung, Bong-Koo ; Ryou, Na-Young ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 93~103
In order to find out effect on the inorganic and organic compounds as a soil amendment to control Fusarium wilt of cucumber caused by Fusarium oxysporum f. sp. cucumerinum, this study was conducted during the last two years from 1993 to 1995. According to add 14 inorganic chemicals (1%, w/v) including
individually in vitro, these chemicals were confirmed their suppression, and especially Alum, CaO and
suppressed not only
percent on mycelial growth of the fungus, but also inhibited
on conidial germination.
suppressed mycelial growth only, while KCl,
, and Urea suppressed conidial germination. The 7 chemicals were finally seleced. Composted pine bark (CPB) suppressed definely more than 90% on conidial germination in the different extract concentration (2,5 and 10%), although mycelial growth on extract medium of CPB and milled alfalfa leaves (MAL) were not remarkable. The antagonist Trichoderma sp. (Tr-3) mixed with an amended soil (1%, w/w) containing composted pine bark showed a good mycelial growth to compete the causal fungus. And the antagonist Pseudomonas sp. (7-1-3) was also confirmed its antagonistic ability with culture filterate. It is known that a CPB soil amendment mixed with the two antagonists (1%, w/w) controlled almost completely Fusarium wilt of cucumber in greenhouse pots and a field experiment. It is therefore expected that biocontrol on Fusarium wilt of cucumber by a soil amendment can be applied to farmmer's fields.
Studies on the anti-complementary polysaccharides produced from submerged mycelial culture of Pleurotus sajor-caju
Song, Chi-Hyeun ; Kim, Jae-Hong ; Yang, Byoung-Kun ; Kim, Kil-Woong ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 104~110
The effect of nutritional and cultural conditions for the production of anti-complementary polysaccharide from Pleurotus sajor-caju was studied. Maximum anti-complementary activity from its culture broth and mycelium was obtained by using lactose and maltose as a carbon source, respectively. Ammonium phosphate (monobasic) and serine were chosen for the highest activity among the inorganic nitrogen compounds and amino acids tested. Optimum temperature for the production of the anti-complementary polysaccharide was
Intergeneric Transfer of Isolated Nuclei from Lentinus edodes into Protoplasts of Pleurotus florida
Yoo, Young-Bok ; Shin, Pyung-Gyun ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 111~126
Transfer of the isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida was induced with polyethlene glycol (PEG) and
. The intergeneric transfer products were classified into nuclear hybrid, heterokaryon or synkaryon, and reconstituted cell. These progenies except nuclear hybrids formed mature fruiting bodies on sawdust rice bran medium. Formation of fruit bodies was influenced by several factors such as light, temperature, nutrition and physic state of the culture media. Most of fruiting body characters were similar to those of P. florida in synkaryon and L. edodes in reconstituted cell, respectively. All these basidiocarps had clamp connections though initial heterokaryon colonies were lacking. Isozyme patterns of intergeneric progenies were quite different from those of parents. DNA polymorphisms of transfer products were also compared by random amplified polymorphic DNAs (RAPD) analysis based on polymerase chain reaction. The RAPD patterns were different from those of donor and recipient. DNA fingerprints ranged in size from 0.25 to 4.0 Kb. On the basis of RAPD, the transfer products were classified into five groups. Two synkaryon were analysed with distribution of progenies and segregation of genetic markers by random spore analyses. The genetic markers were segregated into wild type and riboflavine requiring auxotrophs.
Studies on collection and spawn manufacture of Armillaria spp. for production of Gastrodia tuber
Sung, Jae-Mo ; Jung, Beom-Sig ; Moon, Hee-Woo ; Kim, Su-Ho ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 127~134
Armillaria isolates (KNU-A110, KNU-A234, KNU-A1022 and KNU-A1030) were excellent isolates for producing Gastrodia tuber in farm cultivation. Depth of soil between
was favorable for producing Gastrodia tuber and rhizomorph at cultivated area. Eighty nine isolates were collected from 5 countries; 16 from Japan, 22 from USA, 26 from France, 4 from Africa and 21 from Korea. Mycelial fan of most isolates were better formed on basal medium with lemon extract than without lemon.A. mellea (KNU-A997) were strongly pathogenic to Gastrodia but A. gallica (KNU-A110) were excellent symbiotic to Gastrodia tuber. Mycelial growth were good on basal medium containing
ethanol and sawdust spawn added wheat bran and corncob. Liquid culture inoculation were not only fast growth of mycelium but also reduction of contamination.
Isolation of auxotrophs and drug resistant mutants of Lentinus edodes
Kim, Chae-Kyun ; Shim, Mi-Ja ; Choi, Eung-Chil ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 135~141
Auxotrophs and drug resistant mutants from the mycelia of Lentinus edodes were obtained by UV irradiation at survival rates of
and ethidium bromide (EtBr) enrichment after UV irradiation. The mutation rate was 0.40%, and back mutation rate was
. Various amino acid-, nucleic acid-, and vitamin-requiring auxotrophs were isolated. The concentrations of several fungicides, antibiotics and amino acid analogues inhibiting the growth of L. edodes were determined. The MIC values for cycloheximide, benomyl, and p-fluorophenylalanine were 2, 2000, and 1000 ug/ml respectively. Five p-fluorophenylalanine-resistant mutants and eight benomyl-resistant mutants were selected by UV irradiation.
Studies on immunomodulating function of components separated from higher fungi
Bae, Man-Jong ; Park, Mu-Hee ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 142~148
To find compounds of immunomodulating and anti-allergic function, effects of protein-bound polysaccharides extracted from Phellinus igniarius (PI), Fomitella fraxinea (FF) and Agrocybe cylindracea (AC) on hemagglutinin titer (HA), hemolysin titer (HY), plaque forming cell (PFC), rosette forming cell (RFC) and phagocytosis were investigated in BALB/C mice. The oral administration of the protein-bound polysaccharides of PI, FF and AC for 10 days resulted in the enhanced phagocytic activity of peritoneal exudate cells (PEC), spleen cells (SC) and blood lymphocyte cells (BLC). Moreover, PI showed the activating effect on the phagocytosis of PEC and AC in SC. In the experiment of PFC and RFC, the results of the experimental group which was given each samples as compared to the control group, showed the enhanced level of activity such as PI 130%, FF 90% and AC 70%. Generally, HY and HA showed from ten to hundred times of level in each sample groups, as compared to the control group.
Changes in activities of protease, phenoloxidase and cellulase during mycelium growth of Pleurotus ostreatus in sawdust cultures
Chang, Hyun-You ; Kim, Gwang-Po ; Cha, Dong-Yeul ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 149~154
Effects of various kinds of sawdusts, supplements and culture conditions on activities of several enzymes such as protease, phenoloxidase and cellulase produced from mycelium of P. ostreatus grown on sawdust medium were studied and the results are as follows; Higher specific activity of these enzymes was observed when oak tree sawdust and poplar tree sawdust were supplemented with rice bran or wheat bran at rate of 30%, 20% and 10% in total volume respectively. Higher total activities of protease, phenoloxidase and cellulase were observed at 70% of the moisture contents of culture media, while lower activity of these enzymes was observed with 40% moisture contents of sawdust culture medium. The pH 4 and 9 of the sawdust media appeared to be optimum pH for the. production of protease while pH 5 and 7 were optimal for the production of phenoloxidase. The pH 6 of the sawdust medium was optimal for the production of cellulase. The optimum incubating temperature for the production of protease, phenoloxidase and cellulase was
. Higher total activities of protease and phenoloxidase were observed when culture medium was added with wood vinegar at the control, and 0.5% for cellulase.
Phylogenetic Relationships Among Pleurotus species Inferred from Sequence Data of PCR Amplified ITS II Region in Ribosomal DNA
Bae, Shin-Churl ; Seong, Ki-Young ; Lee, Shin-Woo ; Go, Seung-Joo ; Eun, Moo-Young ; Rhee, In-Koo ;
The Korean Journal of Mycology, volume 24, issue 2, 1996, Pages 155~165
This study was carried out to identify the phylogenetic relationship among several isolates of Pleurotus species by comparing ITS II region of ribosomal DNA(rDNA) repeat unit. Two primers from ribosomal DNA sequences were chosen to amplify the specific internal transcribed spacer (ITS) II region of Pleurotus spp. The exact ITS II region with an unique band from six species of Pleurotus genus could be amplified using the two primers taken from at the 3'-end of 5.8S rDNA and 5'-end of 28S rDNA. Six representative species of the Pleurotus genus were easily characterized according to the length differences of ITS II region. Furthermore, within P. ostreatus species, different sizes of ITS II region could be observed in the isolates of ASI 2025 and ASI 2095 although they were classified as P. ostreatus by the conventional observation. The nucleotide sequence analyses of PCR-amplified ITS II region indicated that the isolates ASI 2025 and ASI 2095 were different from other Pleurotus spp. When the nucleotide sequences of six Pleurotus species were compared, three typical ITS II regions were highly variable especially at both ends of this region. The phylogenetic tree obtained by the Neighbor program of Felsenstein PHYLIP package with all the nucleotide sequence of Pleurotus spp. indicated that P. ostreatus, P. florida, P. sajor-caju and P. eryngii were closely related to one phylogenetic branch and P. cystidious was related to other branch with P. cornucopiae. The isolates ASI 2025 and 2038, however, were not closely related to any other Pleurotus spp. and formed their own individual branches.