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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 1996
Volume 24, Issue 3 - Sep 1996
Volume 24, Issue 2 - Jun 1996
Volume 24, Issue 1 - Mar 1996
Selecting the target year
Studies on the Inheritance of fruitbody color in Flammulina velutipes
Byun, Myung-Ok ; Kong, Won-Sik ; Kim, Young-Ho ; You, Chang-Hyun ; Cha, Dong-Yeul ; Lee, Du-Hyung ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 237~245
Esterase isozyme band patterns were compared between the wild strain and commercial strain of Flammulina velutipes. Monospores were isolated from wild strain. ASI4019 and their mating types were determined. We investigated the relationship between pigmentation on the plate and fruitbody color to understand genetic relationship among F. velutipes strains. Dikaryotic strains mated between nonpigmenting strains produced white fruitbodies. However dikaryon obtained from mating between nonpigmenting monokaryon and brown pigmenting monokaryon produced brown fruitbody as the dikaryon obtained from mating of brown pigmenting monokaryons. The white fruitbody from wild strain was distinguished from that of commercial strain. When the nonpigmenting wild monokaryon was mated with commercial monokaryon, pale brown mushroom was produced. The BC1F1 was obtained by mating the above mentioned
with commercial monokaryon. Fruitbody color of BC1F1 shared two types; one strain with all pale brown fruitbodies, and the other strain with separated eight pale brown and two mixed type involving pale brown and white fruitbodies.
Xylogone sphaerospora, a New Fungal Pathogen of Cultivated Ganoderma lucidum
Lee, Jong-Kyu ; Choi, Gyung-Ja ; Cho, Kwang-Yun ; Oh, Se-Jong ; Park, Jeong-Sik ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 246~254
Since the mid of 1980's, cultivation area and production of Ganoderma lucidum have been increased annually in Korea. However, the presence of a fungal disease has become a major limiting factor in the cultivation of Ganoderma lucidum, causing a serious economic loss. The present study was carried out to isolate and identify the pathogenic fungus to Ganoderma lucidum. Several fungi isolated from the wood logs showing typical symptoms were tested whether they are pathogenic to Ganoderma lucidum or not by cross-pairing culture method, flask inoculation method, and wood log inoculation method. The pathogenic fungus produced ascomata. Mature ascomata was spherical, dark, thick-walled,
diameter. Asci were thin-walled, evanescent when mature, disintegrate early. Ascospores were spherical, hyaline, glaborous, thick-walled, refractive,
in size. Conidiophores soon became abundantly septate and broke up into arthrospores, which are cylindrical,
wide. Based on the observations under dissecting microscope, light microscope and scanning electron microscope, teleomorph and anamorph of the pathogenic fungus were identified as Xylogone sphaerospora Von Arx & Nilsson and Sporendonema purpurascens (Bonordon) Mason & Hughes, respectively. X. sphaerospora is first reported as a pathogenic fungus of Ganoderma lucidum.
Mode of Action and Chemical Modification of an Alkaline Xylanase (CX-III) from Alkalophilic Cephalosporium sp. RYM-202
Kang, Myoung-Kyu ; Maeng, Pil-Jae ; Rhee, Young-Ha ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 255~264
The hydrolysis products formed from birchwood xylan by the action of an alkaline xylanase (CX-III) from alkalophilic Cephaloxporium sp. RYM-202 were xylobiose and xylooligosaccharides polymerized with more than 4 sugar molecules. This enzyme was not active on xylobiose but readily attacked xylotriose accumulating xylobiose as a major product. The predominant end-products from xylotetraose by CX-III were xylobiose and xylotriose. These results indicate that the enzyme is typically endo-type xylanase possessing transglycosidase activity. Chemical modification of CX-III with N-bromosuccinimide revealed that two tryptophan residues per molecule of CX-III were essential for its catalytic activity on xylan. On the other hand, iodoacetamide and diethylpyrocarbonate did not influence the activity of the enzyme, suggesting that cysteine and histidine residues are not involved in the active site of this alkaline xylanase.
Taxonomic study on Korean Aphyllophorales (III) -on some unrecorded corticioid fungi-
Jung, Hack-Sung ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 265~273
Fleshy fungi were collected during field trips to mountain areas throughout the country from October in 1994 to June in 1995. Through the observation and identification of specimens belonging to the wood-rotting fungi of the Aphyllophorales, one genus, Phlebiopsis, and six species, Athelia bombacina, Ceraceomyces sublaevis, Hyphoderma sibiricum, Hyphodontia arguta, Phlebiopsis gigantea, and Radulomyces confluens were confirmed as new corticioid fungi to Korea and are registered here with descriptions.
Sawdust cultures of Lepista nuda
Lee, Sang-Sun ; Choi, Kyoung-Jin ; Oh, Chang-Ho ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 274~279
An isolate of Lepista nuda was cultivated on the solid media consisted of various cereal grains or those of the acacia sawdust mixed with other cereal grains. The mycelia grew well on the solid cultures made from millet cereals, but did not on those from sorghum or corn cereals. Thus, the millet and the acacia sawdust, as the solid media, were employed for the next experiments related to mycelial growths. For growth of this isolate, it was shown that the nitrogen source is needed for those of millet. Also, the rice bran or minerals were considered to be needed for the acacia sawdust. As minerals, however, the rice bran is shown as the best additive in the solid cultures of the acacia sawdust. The mycelia of L. nuda were cultivated directly through the solid cultures. Their growths were measured' by the production of carbon dioxide with gas chromatography.
Studies on the Fungal Isolates of Mucorales Collected from Korean Home Made Mejus and Nuluks
Yu, Kee-Won ; Seoung, Chang-Kun ; Lee, Sang-Sun ; Yoo, Jin-Young ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 280~292
The fungal isolates of Mucorales, directly collected from Korean traditional raw materials of Nuruk (raw material for Korean rice wine) and Meju (raw material for Korean soysauces), were compared with those of Rhizopus oryzae purchased. The fungal isolates of Rhizopus, Mucor, and Absidia mostly identified as based on the morphological observations, were evaluated with the PCR-polymorphic bands. The PCR-polymorphic bands of the genomic DNA reacted with the primers of OPD series tenmer were various, but showed averaged 4 to 6 in the agarose-electrophoresis. The dissimilarity coefficient (DC) between two isolates were compared by the cluster analyses, dendrogams and polar ordinations. The isolates of R. oryzae known. showed several groupings within the lower value of DC and were divided to two groups of amylo-process and other fungi with other purposes. The isolates unidentified were identified by the DC made of this results. Taxonomy of these isolates made by the morphological observations were consistent with those resulted above in most case but not in all aspects. More works were needed with the isolates known for detail informations of Mucorales.
Purification and characterization of antifungal compounds produced by Bacillus subtilis KS1
Ryoo, Sung-Woo ; Maeng, Hack-Young ; Maeng, Pil-Jae ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 293~304
A bacterial strain, KSl, possessing strong antifungal activity was isolated from soil samples of ginseng fields and identified as Bacillus subtilis. In greenhouse test, the culture filtrate of B. subtilis KS1 showed strong protective effect against several fungal diseases of agricultural plants such as cucumber gray mold and wheat leaf rust. In addition, the crude butanol fraction of the culture filtrate exhibited antagonistic effect against several fungi including plant or human pathogens, such as Botrytis maydis, Chytridium lagenarium and Candida albicans. The antifungal compound, SW1, produced by B. subtilis KS1 was purified through consecutive chromatographic separations on a pep-RPC column and a
reverse phase column. Temperature and pH showed little effect on the stability of the compound in the ranges
and pH 4.0-10.0, respectively. The composition and structural characteristics of SW1 were analysed by HPLC and by
, NOESY, COSY-NOESY and HOHAHA NMR spectroscopy, respectively, which revealed that the compound belongs to iturin A, a typical cyclic antifungal compound produced by B. subtilis. In contrast to the previously reported iturin A compounds which have one or no
side chain in the hydrophobic hydrocarbon chain of
acids, SW1 was shown to have a
acid containing 12-carbon skeleton with two
The Production of Mushroom Mycelium Using Rancid Oil of Fried Chicken
Jung, Gi-Tai ; Kim, Kyu-Tae ; Choi, Joung-Sik ; Hong, Jai-Sik ; Kim, Kum-Jae ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 305~309
The conditions for mycelial production of mushroom rising rancid oil of fried chicken were investigated. Pleurotus ostreatus Nonggi 202 which showed the greatest mycelial growth among mushrooms was chosen for the investigation. The optimum temperature and pH for mycelial growth were
and 5.5 to 6.5, respectively. Tryptone was better effective than any other nitrogen sources on the mycelial growth. The mycelial growth was enhanced with addition of 0.3%
. and 0.02%
, respectively. Among the vitamins, thiamine was the most effective on the mycelial growth.
Biological and Physico-chemical Properties of Antifungal Cyclic Lipopeptides Produced by Pseudomonas cepacia Strains
Kim, Sung-Ho ; Lee, Min-Woong ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 310~321
Five strains AF027, AF069, AF2001, AF2011 and SD02 of Pseudomonas cepacia were isolated from soil, and the antifungal cyclic lipopeptides(CLP) i.e, CLP027A, CLP069A, Cepacidine A, CLP2011A and CLP02A were produced from each strains, respectively. Nitrogen and carbon sources in media were proved to be important factors for the production of CLP and among them, polypeptone-S, glucose and fructose were the most effective. It appeared that compounds CLP027A and CLP069A were identical with Cepacidine A and Xylocandine A, respectively. contain aspartic acid as amino acid component, are differentiated from Xylocandine A containing asparagine. Although molecular weight, amino acid composition and UV spectrum of CLP2011A and CLP02A are same with those of Cepacidine A, it is postulated that these compounds are not identical with Cepacidine A when the antifungal spectra and antifungal activity were compared to those of Cepacidine A.
The Effect of Reynoutria sachalinensis on Mycelial Growth of Pleurotus ostreatus
Park, Won-Mok ; Kim, Young-Ho ; Ko, Han-Gyu ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 322~328
It is possible to cultivate Pleurotus ostreatus on the crush medium of Reynoutria sachalinensis. The crush of leaves and stems of Reynoutria sachalinensis with water (1:8 W/V) enhanced mycelial growth of P. ostreatus. That mycelial growth of P. ostreatus on the crush medium was accelerated three times as fast as that on malt extract agar (MEA), and mycelial compactness was denser than that of on MEA. The same result was obtained on mixture of saw dust and the crush of leaves and stems in test tube and bottle. The addition of rice bran and the crush to saw dust was best for mycelial growth. Regardless of pH (4.5, 6.5 and 8.5), P. ostreatus could suppress the growth.
The fungal isolates of Scopulariopsis collected from Korean home-made Mejus
Lee, Sang-Sun ; Yoon, Young-Sil ; Yoo, Jin-Young ; Lee, Kap-Duk ;
The Korean Journal of Mycology, volume 24, issue 4, 1996, Pages 329~336
From five years' previous work, the fungal isolates of Scopulariopsis were reported to be important flora at the late stage of meju fermentation. Mainly, the white or pale brown powders of spore mass of these fungi were observed on the surfaces of rectangular mejus, and to be an important sign for well-done Korean traditional home - made mejus. Out of the five isolates previously collected and stored, two kinds of Scopulariopsis isolates were identified as S. brevicauli and S. fusca. The microscopic differences between two were found to be branching patterns of annellophore and ornamentations of spore wall (warty and smooth). However, the intermediate form between two ornamentations of spore wall were also observed in our isolates. This observation was consistent with other result made from the protein electrophoresis. The isolates of Scopulariopsis were considered to be similar or superior to those of Aspergillus species, as compared with production of protease and amylase related enzymes. Thus, these isolates were speculated to be important fungi in Korean traditional home - made meju fermentation and also in production of protease and amylase.