Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 25, Issue 4 - Dec 1997
Volume 25, Issue 3 - Sep 1997
Volume 25, Issue 2 - Jun 1997
Volume 25, Issue 1 - Mar 1997
Selecting the target year
Lichen Flora of Ullung Island(I) -Graphis and Pyrenula Genera-
Ka, Kang-Hyeon ; Park, Hyun ; Ryoo, Cheon-In ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 77~84
The samples of 26 lichens were collected during two field trips to Ullung Island in May and October, 1995. From the samples collected, three species in two genera were identified; Graphis rikuziensis (Vain.) Nak., G. scripta (L.) Ach. and Pyrenula japonica Kurok. Among them, P. japonica was identified for the first time in Korea and is described here in detail.
Electrophoretic Patterns of Isozymes from the Mycelia of the Auxotrophs of Lentinula edodes
Kim, Chae-Kyun ; Kim, Byong-Kak ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 85~90
The Isozyme activities of Lentinula edodes were studied as a preliminary study for genetic analysis after protoplast fusion. The presence of peroxidase, esterase, superoxide dismutase, acid phosphatase, alkaline phosphatase, alcohol dehydrogenase and
was examined. An intracellular buffer-soluble protein from the mycelia was used for enzyme analysis on nondenaturing polyacrylamide gels. The auxotrophs of Lentinula edodes were positive for peroxidase, esterase, superoxide dismutase and acid phosphatase. However, alkaline phosphatase, alcohol dehydrogenase and
were not detected. The esterase and peroxidase were not affected by the various culture age. Isozyme identification may be a useful tool after protoplast fusion.
Production of Alkaline Carboxymethyl Cellulase and Xylanase by Batch and Fed-batch Cultures of Alkalophilic Cephalosporium sp. RYM-202
Kang, Myoung-Kyu ; Kim, Do-Young ; Rhee, Young-Ha ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 91~100
Production of alkaline carboxymethyl cellulase (CMCase) and xylanase by batch and fed-batch cultures of alkalophilic Cephalosporium sp. RYM-202 was investigated. Of carbon sources tested, wheat bran gave the highest production of those enzymes. The high levels of CMCase on carboxymethyl cellulose and xylanase on birchwood xylan suggest that the biosynthesis of CMCase and xylanase in Cephalosporium sp. RYM-202 is regulated separately at the level of enzyme induction. The temperature and pH for maximal production of those enzymes was
and 9.0, respectively. High concentration of wheat bran in batch fermentation resulted in the lower and delayed production of the enzymes by catabolite repression. In fed-batch fermentation with controlled feeding of 5% final wheat bran concentration, the highest activities of CMCase and xylanase were 0.39 and 9.2 units/ml, respectively, and 1.22 and 1.36 times higher respectively than those in batch fermentation on 5% wheat bran.
Isolations of Orchid Mycorrhizal Fungi from the Korean Native Orchid Plants
Lee, Sang-Sun ; Riew, Hee-Kyun ; Paek, Kee-Yoeup ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 101~110
This study was to identify the orchid mycorrhizal fungi and to test whether the orchid plants antificially inoculated with this fungus showed better growth them uninoculated plants. Symbioses in the root cells of the native plants of Cymbidium goeringii collected were observed and the digestive forms of peletons were also observed in various native roots. Two types of hyphae, thick
in thickness, were conclusively found to be from various native orchid roots. The symbiotic fungus was isolated by several agars and identified as a Rhizoctonia repens or a R. endophytica var. endophytica. Symbioses on the plantlets of C. karnan and Cymbidium hybrid 'Onomoron' were evaluated as the isolates inoculated on oatmeal agars. The growth of plantlets were measured with the formations of mycorrhizae in the roots. R. repens was shown to be the better isolate than the other in growth stimulation of plantlets on oatmeal agars when grown for two months. The two types of hyphae in the root cells under nature were speculated from the different fungal isolates of Rhizoctonia. Further isolates would be needed for application works for the orchid industries.
Genetic Variability of Flammulina velutipes Monosporous Isolates
Kong, Won-Sik ; Kim, Dong-Hyun ; Kim, Young-Ho ; Kim, Kyoung-Soo ; You, Chang-Hyun ; Byun, Myung-Ok ; Kim, Kwang-Ho ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 111~120
The characteristics of monosporous isolates of winter mushroom [Flammulina velutipes (Curt. ex Fr.)] were investigated to obtain useful breeding materials. Within monokaryons from cultivars which have a white colored fruitbody showed narrow genetic variation, while domestic strains which have a brown colored fruitbody showed wide variation. The mating type of the white strains was A1A2B1B2 genotype, but that of the domestic brown strains were A3A4B3B4. In intra-crossing, the genetic stability of dikaryons mated by monokaryons from white strain was less than that of parents. While in brown strain, dikaryons with high yield and color variation were obtained.
The Mycelia Isolated from the Basidiocarps of Tricholoma matsutake in Korea
Lee, Sang-Sun ; Sung, Chang-Kun ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 121~129
Tricholoma matsutake (TM=T. caligatum var. nauseoum) is, for an agricultural income earned by Forestry by-products, a very important mushroom in Korea. The mycelia isolated from the basidiocarps were compared with basidiocarps of TM by the random amplified polymorphisms of RAPD-DNA bands. The mycelia were confirmed to be originated from the basidiocarps of TM by cluster analyses of the DNA-bands made from RAPDs and Southern blotting with the band (0.75 kb) identified. The mycelia defined were observed to grow very slowly at the rate of 10 cm per month at
and also to be semi-transparent and submerged in on PDA. The method developed in this work was considered to be very useful for confirming the mycelia originated from the ectomycorrhizal mushrooms and also to be applied for the fungal mycelia isolated from the commercial useful mushrooms.
Artificial Cultivation of Phellinus linteus
Song, Chi-Hyun ; Moon, Hye-Yeon ; Ryu, Chung-Hyun ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 130~132
Artificial cultivation of Phellinus linteus was studied. The best yield (B.E.
) of basidiocarps including basidiospore was obtained using Quercus acutissima among the logs of Morus alba, Quercus acutissima, Alnus japonica, Castanea crenata. Optimum temperature and required periods for the basidiocarps formation were
and about 10 months, respectively.
Cultural Condition for the Mycelial Growth of Phellinus igniarius on Chemically Defined Medium and Grains
Jung, In-Chang ; Kim, Seon-Hee ; Kwon, Yong-Il ; Kim, So-Yeun ; Lee, Jong-Suk ; Park, Shin ; Park, Kyung-Sook ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 133~142
The chemical media composition and culture conditions were optimized for mycelial growth of Phellinus igniarius 26005. The method of solid-state fermentation, cultivation of basidiomycetal strains in various grains, was developed. Media composition for optimal growth of Phellinus igniarius 26005 was made of 7.0% malt extract, 0.3% bacto soytone, and 0.2% yeast extract. The optimum condition for mycelial growth was
and pH 7.0, respectively. For the mass cultivation of mycelia, the hydrated grains with cold water, were put into the plastic bottle. The mycelial growth rate in the bottled grains was high in the early stage with inoculation of homogenized mycelium. The activity of mycelium was maintained by adding sterilized water in the middle of cultivation. The glucosamine content which determins the mycelial growth rate in solid material was in the order of job's tears>barley>black soybean>wheat>malt soybean>brown rice>sorghum>glutinous rice.
Development of Lipase Hyper-producing Strain from Hybrids between Aspergillus niger and Penicillium notatum by Nuclear Transfer
Yang, Young-Ki ; Moon, Myeng-Nim ; Lee, Yoon-Hee ; Kang, Hee-Kyoung ; Lee, Jung-Sup ; Lim, Chae-Young ; Kim, Jong-Se ; Rhee, Young-Ha ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 143~151
Interspecific hybrids between Aspergillus niger and Penicillium notatum (Tyr-), hyperlipolytic enzyme-producing fungi, were obtained by nuclear transfer technique. Optimal conditions for formation of intergeneric hybrids were investigated. Maximum production of protoplasts was obtained by 1% Novozyme 234 at
for 3 hrs and the most effective osmotic stabilizers for the isolation of protoplasts were 0.6 M KCl. Frequencies of hybrid formation by nuclear transfer were
. From the observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyotypes are aneuploid. The hybrids showed
fold higher lipase activities than parental strains. It was strongly supported by results of this study that nuclear transfer technique is much more efficient in the formation of intergeneric hybrids than protoplast fusion and is very useful for the improvement of strains.
The Genetic Characteristics of Strains and the Optimal Condition for Mycelial Growth of Naematoloma sublateritium on Sawdust Media
Kang, An-Seok ; Kong, Won-Sik ; Seok, Soon-Ja ; Hong, In-Pyo ; Cha, Dong-Yeol ; Kim, Kwang-Po ; Kim, Dong-Hyun ; Yu, Seung-Hun ;
The Korean Journal of Mycology, volume 25, issue 2, 1997, Pages 152~160
In order to compare collected strains of Naematoloma sublateritium, electrophoretic isozyme and RAPD band patterns of DNA from mycelia were studied. The two characteristics of the strain, ASI 11010 were much different from those of ASI 11005 and 11006. These patterns may be used as valuable criteria for identifying strains of N. sublateritium. Optimal conditions on the vegetative growth of N. sublateritium using sawdust media were favourable on alder sawdust media added with 30% beer waste. Optimal water content was 70%.