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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 26, Issue 4 - Dec 1998
Volume 26, Issue 3 - Sep 1998
Volume 26, Issue 2 - Jun 1998
Volume 26, Issue 1 - Mar 1998
Selecting the target year
Classification of Cordyceps Species Based on Protein Banding Pattern
Sung, Jae-Mo ; Lee, Hyun-Kyung ; Yoo, Young-Jin ; Choi, Young-Sang ; Kim, Sang-Hee ; Kim, Yong-Ook ; Sung, Gi-Ho ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 1~7
In order to find relationship within and between entomopathogenic species, analysis of protein band pattern in mycelia of 25 isolates was conducted by UPGMA. The results allowed differentiation of three groups on 85% similarity coefficient. Similarity coefficient within C. militaris was
, C. kyushuensis was 0.958-1.000 and C. pruinosa was 0.993-1.000. C210 and C298 isolates which had somewhat immersed perithecia, comparable to other C. militaris isolates, had 91% similarity. C108, C225-1 and C228 isolates pathogenic on Lepidopterous larvae had 89% similarity. Closely related species to C. militaris were C. kyushuensis and C. pruinosa. And similarity between C. pruinosa and C. kyushuensis was 88%. Similarity between C. bifusispora formed conidia on media and Paecilomyces tenuipes was 89%. C. scarabaeicola pathogenic specifically on adult Scarabaeidae had 82% similarity with above two species. C118 identified as C. militaris showed different protein banding patterns.
Decolorization of Azo, Triphenylmethane and Heterocyclic Dyes by Irpex zonatus BN2
Yoon, Kyung-Ha ; Choi, Yang-Soon ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 8~15
The present research was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with Irpex zonatus BN2, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low
, its laccase activity was very high
. Moreover, laccase had appeared earlier than MnP. When the isolate was incubated with each dye for 10 days, the decolorization rates of azo dyes, such as orange II, orange G, tropaeolin O and congo red were 98.0%, 97.4%, 99.0% and 95.3%, respectively. In case of heterocyclic dyes, eosin Y, toludine blue, methyl blue and azur B were 97.4 %, 98.7%, 99.9% and 94.0% respectively. Finally the results of triphenylmethane dye such as basic fuchsin, malachite green and crystal violet were 98.5%, 95.7% and 99.4%, respectively. The results suggest that laccase of Irpex zonatus BN2 should be played an important role in the decolorization of the dyes.
Spore germination and Sexuality of Ganoderma
Kim, Kyung-Soo ; You, Chang-Hyun ; Cha, Dong-Yeul ; Ko, Mi-Suk ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 16~19
A study was carried out to explore the method for the germination of the spore of Ganoderma. Three strains of Ganoderma were examined using 2 different media such as PDA and LBA at three different temperatures. As it has been known the germination rate was very low under all the conditions examined. G. lucidum ASI 7004 showed 0.02% germination on both media at
respectively. The germination rate of ASI 7091 was 0.05% on PDA medium at
. The germination rate of G. oregonense ASI 7067 was 0.67% on PDA at
. LBA medium was found to be inadequate for the germination of Ganderma species in this study. In a consequent study, four mating types of G. oregonense such as
Effect of Liquid Spawn on Flammulina velutipes Cultivation
Ryu, Young-Hyun ; Yoon, Young-Seok ; Jo, Woo-Sik ; Park, Sun-Do ; Choi, Boo-Sull ; Kim, Jong-Kuk ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 20~24
The effects of liquid spawn to sawdust substrate on the growth of Flammulina velutipes were conducted for two years. The duration of optimal incubation for preculture and main culture of liquid spawn was 4 days and 6 days, respectively. When using liquid spawn, the application time for the first pinhead formation was similar with sawdust spawn, and incubation time of sawdust substrate was variable with liquid spawn and inoculum quantity. But, the overall yield of mushroom fruitbody was increased by using liquid spawn, excepting sesame hull extract medium.
Artificial Culture Method of Coriolus versicolor (Fr.) Quel. Mushrooms
Jo, Woo-Sik ; Yun, Yeong-Seok ; Rew, Young-Hyun ; Park, Sun-Do ; Choi, Boo-Sull ; Uhm, Jae-Youl ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 25~30
Present expriments were conducted to determine the possibility of cultivation of Coriolus versicolor selected among the higher fungi growing in Korea. Dried apple sawdust used in this study consisted of 31.5% C, 0.54% N, 2.41 % CaO, 0.05%
, 0.48% MgO and 0.81%
(pH 5.6). Coriolus versicolor mushrooms were cultivated on the sawdust media; apple sawdust; rice bran=80:20 in 850cc polypropylene bottles. The isolate of Coriolus versicolor used was YCV collected from an Apple farm in Youngchun district. It took 34 days to make fruitbody from spawning. The fruit bodies produced the total fresh weight 28 g in a bottles, and converted to 64% of fresh weight.
Occurrence and Identification of the Fungus Causing Yellow Rot on Ganoderma lucidum
Oh, Se-Jong ; Chun, Chang-Sung ; Lee, Jong-Kyu ; Kim, Hee-Kyu ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 31~38
Since 1990, destructive yellow rots on Ganoderma lucidum caused by a soilborne fungus have been occurred in major cultivation areas of Korea. Incidences of the disease were 61% in Chulwon and 94% in Kanghwa area where the mushroom has been cultivated for 10 years, whereas the disease has not been found yet in new cultivation areas such as Moonkyung and Hongsung. when severely infected, inner tissues of bed-logs showed severe yellow and fruiting bodies of the mushroom was not produced. Infected tissues of bed-logs were readily distinguished from those of healthy ones by a distinctive brown border line. When the disease progressed, mycelia of Ganoderma lucidum were totally destroyed, and abundant ascocarps of the pathogen were formed inside the tissues of bed-logs showing yellowish green. The fungus derived from a single ascospore strongly lysed mycelia of Ganoderma lucidum growing on bottle media, and non-volatile components secreted by the pathogen were also highly inhibitory to mycelial growth of the mushroom fungus. The pathogen was identified as Arthrographis cuboidea based on its distinctive cultural and morphological characters. The fungus produced arthroconidia and unbrached conidiophores. The width of fungal conidia was distinctively wide as compared with the length. Colonies of the fungi were pale yellow to yellowish green on agar media. As a causal pathogen of yellow rot of Ganoderma lucidum., this fungus has not been reported yet in Korea.
The optimal Factors for the Mycelial Growth of Sparassis crispa
Shim, Jae-Ouk ; Son, Seo-Gyu ; Yoon, Sang-Ook ; Lee, Youn-Su ; Lee, Tae-Soo ; Lee, Sang-Sun ; Lee, Kap-Duk ; Lee, Min-Woong ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 39~46
This study was carried out to obtain the basic data for artificial culture of Sparassis crispa. The optimal conditions for the mycelial growth were
and pH 4, respectively. The mycelial density of S. crispa was most compact in the Hamada media, whereas colony diameter was prominent in the Hoppkins media. Carbon sources such as maltose, arabinose and mannitol were favorable for stimulating a mycelial growth of S. crispa. Glycine, one of nitrogen sources also appeared to be favorable to the mycelial growth. The optimum C/N ratio was about 20 : 1 in case that 1% glucose as carbon source was mixed with the basal medium. Fumaric acid or lactic acid as organic acid was most favorable to the mycelial growth.
Bottle Cultivation of Pleurotus ostreatus and Agrocybe aegerita using Agricultural by-product
Lee, He-Duck ; Kim, Yong-Gyun ; Kim, Hong-Gyu ; Han, Gye-Heng ; Moon, Chang-Sick ; Hur, Il-Bum ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 47~50
The cheaper and high-quality media are necessary to produce the year-round mushroom. For low input and high productivity, this experiment was carried out to screen the proper supplementary materials on media among agricultural by-products; bean-curd residues, dried orange peel, chinese drug residues and celery cabbage leaf. Judging by chemico-physicals, the used agricultural by-products was possible for an additives on mushroom media. Bean-curd residues added to media raised the amount of organic matter, nitrogen and carbon. In Pleurotus ostreatus, the conventional media yielded 20 pilus cap, 72g per a bottle, but adding 10 percent bean-curd residues produced 12 pilus cap, 77g and an increase yielded 7%, and 10 percent orange peel took 12 pilus cap and an increase yieled 11%. In Agrocybe aegerita, added to media, except chinese drug residues, increased performance compared with conventional media, produced 98g per a bottle. Especially, the bean-curd residue increase 15 percent in productivity.
Studies on the Development of Casing Materials Using Sawdust Bottle Culture in Cultivated Mushroom, Agaricus bisporus
Kim, Hong-Kyu ; Lee, He-Duck ; Kim, Yong-Gyun ; Han, Gyu-Heng ; Moon, Chang-Sik ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 51~55
After bottle culture of Pleurotus ostreatus, sawdust was taken out from the bottle and accumulated in the middle of March, and then composted. As the result, Y value was decreased rapidly 30 days after composting, and it was decreased slowly after 30 days. It is considered that 118 days is required for composting, however, it is possible to use for casing material after at least 48 days composting. The pH and total nitrogen content of sawdust based on composting period had tendency to increase as composting was processed. Total carbon and C/N rate had tendency to decrease as time went on. Based on the rate of 10, 30 and 50%, each sawdust was added to clay loam used as casing material for culturing A. bisporrus. Among various treatments, the mycelial growth of A. bisporus was more favorable in the treatment of 30% sawdust than in the single treatment of clay loam. Based on the date necessary for primodium formation of A. bisporus, the primodium formation in the treatment of 30% sawdust was reduced to about 5 days as compared with that of any other treatments When 30% sawdust was added to clay loam used as casing material for culturing A. bisporus, the yield of its fruiting body was increased to 28%.
Mycelial Growth of Phellinus linteus with Various Sawdusts
Chi, Jeong-Hyun ; Ha, Tae-Moon ; Kim, Young-Ho ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 56~59
This study was carried out to obtain the basic data on artificial culture of Phellinus linteus. The suitable sorts of sawdusts artifical culture for Phellinus linteus were an oak sawdust, a mulberry sawdust, a peach sawdust. Rice bran, wheat bran and corn seed peel were found to be effective additives, and optimun mixing ratio of an oak sawdust and additives was from
percent. The suitable moisture content of sawdust media was obtained in the range of
. The mixture of percent oak sawdust with 20 percent rice bran in polypropylene (850 cc) bottle was suitable for starter production, and it took 55 days to produce the starters at
Effect of Inoculation Time and Population Density of Pseudomonas agarici and P. tolaasii on the Mycelial Growth and the Fruit body Formation in Flammulina velutipes
Lee, Hyun-Uk ; Moon, Byung-Ju ; Lee, Heung-Su ; Cha, Heung-Oh ; Cho, Dong-Jin ; Shin, Won-Kyo ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 60~68
The effect of P. agarici and P. tolaasii causing the bacterial disease of mushrooms on the mycelial growth and fruitbody formation of F. velutipes was evaluated in laboratory. When the pathogenic bacteria was inoculated simultaneously with F. velutipes or 5 days after inoculation of F. velutipes, they significantly deterred both mycelial growth and fruitbody formation of F. velutipes in sawdust culture and showed strong inhibition under high population density. They appeared to be tender or milky in exhibiting symptom on F. velutipes by inoculating the concentration of
of unit/g media, and their growth seemed to be stopped under
media of P. agarici and
media of P. tolaasii, there was no effect on the fruitbody yield of F. velutipes. P. tolaasii was more suppressive in the mycelial growth of F. velutipes than P. agarici, while on fruitbodies formation of F. velutipes, P. agarici showed slightly higher inhibition than that of P. tolaasii. When the bacteria was inoculated 10 days after inoculation of F. velutipes, both mycelial growth and fruit body formation were not affected nearly.
Antioxidant Activities of Extracts from Fruiting Bodies of Mushrooms
Park, Sang-Shin ; Yu, Kook-Hyun ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 69~77
Antioxidant activities of 80% ethanol extracts of 63 species of mushroom fruiting bodies were investigated. The ethanol extracts from Daedalea dickinsii, Armillariella mellea, and Fomitella fraxinea showed markedly inhibition on lipid peroxidation in rat liver microsome. The extracts from Daedaleopsis tricolor, Trametes suaveolens, Armillariella mellea, Trichaptium abietinum, Daedalea dickinsii, Fomitella fraxinea, Tylophilus neofelleus, Boletellus obscurecoccineus, and Xerocomus subtomentosus significantly inhibited the hepatic aldehyde oxidase activity, and the extracts from Daedaleopsis tricolor, Armillariella mellea, Daedalea dickinsii, and Fomitella fraxinea slightly stimulated the hepatic SOD activity. These results suggest that Daedalea dickinsii, Armillariella mellea, and Fomitella fraxinea contain the bioactive substances for natural antioxidant and may be useful for development of antioxidant from mushrooms.
Searching for Insecticidal Metabolites Produced by Insect Pathogenic Fungi
Youn, Young-Nam ; Yeo, Woon-Hyung ; Seo, Mi-Ja ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 78~85
During the screening of biological control agents for insect pests in the greenhouse, 70 dead insect-related fungi were isolated and tested insecticidal effect of their culture filtrates was tested. From this studies, CNAB-63 isolate showed strong control effects against the cotton aphids and the two-spotted spider mite as 65.19% and 77.55%, respectively. The insecticidal active compound of CNAB-63 isolate was purified from culture filtrate by silica gel chromotography, thin layer chromatography and HPLC. Purified active compound (CNAB) showed control effects against the two-spotted spider mite with 63.45% at
concentration. However, it did not exhibit antimicrobial activity against fungi and bacteria.
Immuno-stimulating Activity of Phellinus linteus
Song, Chi-Hyun ; Ra, Kyung-Soo ; Yang, Byung-Keun ; Jeon, Yong-Jae ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 86~90
Anti-complementary (immuno-stimulating) activities of the polymers produced from submerged mycelial culture, natural and artificially cultivated basidiocarps of Phellinus linteus were compared. Activity
of polymer extracted from natural and artificial cultivated basidiocarps of P. linteus was 65.77% and 63.94%, respectively, which is not significant difference. However, the activities which were significantly low were found from endo-polymer (41.95%) and exo-polymer (21.87%) produced by submerged culture of P. linteus.
Preservation of Mushroom Cultures in Sterile Distilled Water
Lee, Dong-Hoon ; Kim, Chang-Jin ; Shin, Kwang-Soo ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 91~96
Optimal conditions for the preservation of mushroom cultures in sterile distilled water were investigated. Mycelial disks from 12 mushroom cultures were preserved at room temperature,
using sterile water and 6 cryoprotectants for 12 months. The maximal viability of mycelial disks was observed at room temperature and
with the value of 91.7%. At
, the viability was highest in polyethylene glycol (PEG) and lowest in 10% dimethyl sulfoxide (DMSO). The average mycelial growth was highest for disks preserved at
in PEG. PEG was the most suitable for the preservation at
, although this chemical has rarely been used as a cryoprotectant in fungal culture preservation. The laccase isozyme patterns of the preserved isolate of H. ferrugineum were not changed during preservation at
in sterile distilled water as compared to that of slant culture.
Identification of Mycorrhizal Fungi Identified on Citrus Orchard Soils in The Island of Cheju
Lee, Yong-Se ; Chung, Jong-Bae ; Moon, Doo-Khil ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 97~102
VA-mycorrhizal spores were collected from the 14 citurs orchards of different soil textures and locations in Cheju island. Five species and two kinds of spores were identified as based on the morphological characteristics of the spores; Acaulospora bireticulata, Glomus deserticola, G. geosporum, G. vesiculiferum, and Sclerocystis pachycaulis. Additionally, two kind spores of Acaulospora were also observed but difficult to be identified in this moment. Glomus deserticola and unidentified spores of Acaulospora (brown spores sized 90 to
in diameter) were most frequently observed in the all soil specimens in Cheju, while the other kinds of spores were rarely observed in the soil of Cheju.
The Studies on the Heterogenous Gene Expression in Schizophyllum commune
Park, Dong-Chul ; Kim, Hyun-Jeong ; Kim, Ok-Mi ; Bae, Jun-Tae ; Park, Sun-Hee ; Lee, Byeung-Hun ; Lee, Kap-Rang ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 103~107
The trp2 gene encoding trifunctional enzyme in Coprinus cinereus was investigated the expression in the heterothallic mushroom species. To identify the homology of trp2 gene to Schizophyllum commune and Pleurotus ostreatus, southern hybridization was performed with plasmid pHIONA8 containing C. cinereus trp2 gene as a probe, which resulted in a strong signal indicating an homologous sequence to the chromosomal DNA of S. commune. About 50 transformants per dish was appeared in the complementation test by pHIONA8 using S. commune tryptophan auxotroph as host. In the mating test between transformants and other mating type alleles, the fruiting body of S. commune was formed at
Notes on Some New Species Including Unrecorded Species of the Laboulbeniales (Ascomycotina) Collected in Korea
Lee, Yong-Bo ; Na, Young-Hee ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 108~118
Ten species under three genera of the Laboulbeniales in South Korea are treated in this paper. Of these, Laboulbenia mudeungensis Y. B. Lee on Bembidion lissonotum Bates and Laboulbenia nogodanicus Y. B. Lee on Agonum buchanani Hope and Agonum sp. are new to science. Two species under two genera of Filariomyces and Misgomyces, and four species of Laboulbenia are new to Korea. They are Filariomyces forficulae Shanor on Labidura japonica De Hann, Misgomyces dyschirii Thaxter on Dyschirius ovicollis Putzeys, Chitonomyces iriomotensis Majewski on Laccophilus lewisius Sharp, Laboulbenia egens Speg. on Tachys gradatus Bates, Laboulbenia pedicellata Thaxter on Bembidion thermarum Motschulsky and Laboulbenia philanthi Thaxter on Philonthus wuesthoffi Bernhaur. On the other hand, two species of Laboulbenia are collected from the hosts not previously recorded in Korea. They are Laboulbenia flagellata Peyritsch on Platynus daimio Bates and Laboulbenia stenolophi Speg. on Philonthus longicornis Stenphens.
Characterization of Mitochondrial NADH Dehydrogenase in Lentinus edodes
Kim, Eun-Mi ; Min, Ji-Young ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 119~126
Mitochondria were isolated from Lentinus edodes and properties of the mitochondrial NADH dehydrogenase were studied. Optimal pH, temperature, and thermal stability of the enzyme were estimated to be 7.6,
, and stable for one hour at
. The apparent
for the NADH was 0.33 mM. This enzyme catalyzed to transfer electrons from NADH to ferricyanide, decylubiquinone, and 2,6-dichloro-phenol-indophenol. 0.5 mM antimycin A and 0.01 mM dibromothymoquinone strongly inhibited 87.8% and 76.5% of the enzyme activities. 0.01 mM oligomycin known as an inhibitor of ATPase also strongly inhibited 79.2% of activities. 0.5 mM 5,5'-dithiobis-(2-nitrobenzoic acid) and 1.0 mM N-ethylmaleimide known as a modifier of SH group inhibited 50.4% and 36.7% of activities. 1 mM ethyl 2,4-dihydroxy-6-methyl benzoate and 10 mM orcinol, which had been known as an antibiotics isolated from Umbilicaria vellea according to our previous work, stimulated 68.4% and 48.1% of the enzyme activities.
Isolation and Characterization of Regulatory Mutant for Cellulase Production from Trichoderma reesei QM 9414
Choi, Kun-Ho ; Koo, Youn-Mo ; So, Jae-Seong ;
The Korean Journal of Mycology, volume 26, issue 1, 1998, Pages 127~133
Two regulatory mutants of Trichoderma reesei QM 9414 were isolated by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, and the effects of various inducers on the carboxymethyIcellulose (CMC) and filter paper (FP) production were investigated. Induction of CMCase and FPase production of mutants was shown higher level than wild type strain in 1% lactose minimal broth. When induced by glucose, wild type showed glucose-repression for CMCase and FPase production and mutants showed glucose-derepression. Mutant 1 showed 8.38 fold higher CMCase activity and 5.68 fold higher FPase activity than wild type stain. Mutant 2 showed about 8.42 fold higher CMCase activity and 5.41 fold higher FPase activity than wild type strain. Enzyme activities from the mutants and wild type had the same optimum pH of 4.8 and optimum temperature of