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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 26, Issue 4 - Dec 1998
Volume 26, Issue 3 - Sep 1998
Volume 26, Issue 2 - Jun 1998
Volume 26, Issue 1 - Mar 1998
Selecting the target year
Electrophoretic Karyotyping by PFGE in the Genus Fusarium
Min, Byung-Re ; Jung, Jin-Sook ; Choi, Yong-Keel ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 135~143
Contour-clamped homogeneous electric field gel electrophoresis was used to establish electrophoretic karyotype for 10 species of Fusarium sections Sporotrichiella, Liseola, Gibbosum, Discolor and Martiella. Intact chromosomal DNA was isolated from fungal protoplast and separated under various conditions according to their size in order to improve DNA separation. The numbers of chromosome-sized DNA molecules for individual species ranged from 5-13, with individual chromosomes ranging from 0.78 Mb to 7.20 Mb in size. The total genome DNA size of each species was estimated at about 18.32 Mb to 48.20 Mb. Comparison of karyotype profiles following Southern hybridization analysis with a randomly selected genomic probe of F. oxysporum formae speciales litii was carried out.
Screening of White Rot Fungi with Selective Delignification Capacity for Biopulping
Lee, Jong-Kyu ; Oh, Eun-Sung ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 144~152
To obtain white rot fungi which have selective delignification capacity and can be used in biopulping processes, 94 different wood rotting fungi were screened and the capabilities of selected species were evaluated on deciduous and coniferous wood blocks. White rot fungi, first of all, were selected by simple enzyme tests, i.e., cellulase activity test; phenol oxidase activity test; laccase and peroxidase activity test. Most organisms that gave a positive Bavendamm gave a strongly positive laccase test with syringaldazine whereas most of those that gave a negative Bavendamm test also negative test for laccase and peroxidase, even if some exceptions were noted. Wood decay experiement were carried out to select fungal species with selective lignin-degrading ability by inoculating selected fungi to both wood blocks of Populus tomentiglandulosa and Larix leptolepis. After 12 weeks of incubation, weight losses, lignin losses, and morphological characteristics of the decayed wood were investigated. Almost all fungi tested caused 2 or more times of weight losses in P. tomentiglandulosa than in L. leptolepis, while no weight losses were detected from the un-inoculated wood blocks. Ceriporiopsis subvermispora and Phanerochaete chrysosporium were the best delignifiers for both hardwood and softwood. P. chrysosporium, however, was less effective than C. subvermispora. Bjerkandera adusta and two unidentified spp. caused delignification for only P. tomentiglandulosa. B. adusta caused simultaneous rot of all cell wall components, resulted in thinning of the secondary cell wall layers. Other fungi caused selective delignification resulting in the removal of lignin from middle lamella and separation of cells from each other.
Notes on the Higher fungi in Kangwon-do (I) - on Some Unrecorded Species -
Kim, Yang-Sup ; Seok, Soon-Ja ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 153~162
Some fresh fungi collected at three mountain areas in Kangwon-do in 1996 and dried specimens collected before 1996 were observed and identified. Five species, Lepiota grangei, Baeospora myriadophylla, Mycena acicula, Clitocybe sinopica and Gerronema strombodes were confirmed new to Korea and registered here with full descriptions.
Interspecific Relationships within the Fungal Genus Pleurotus by Isozyme Analysis
Lee, Hee-Kyung ; Yoo, Young-Bok ; Cha, Dong-Yeul ; Min, Kyung-Hee ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 163~172
Thirty six strains of Pleurotus spp., from world-wide nations, were examined for interspecific isozyme variation. A comparison of isozymes in mycelial extracts of the fungal genus Pleurotus was made by polyacrylamide gel isoelectric focusing. A total of one hundred and sixty six bands was resolved from six isozymes. A cluster analysis was done based on the zymograms for esterase, glucosephosphate isomerase, leucine aminopeptidase, malate dehydrogenase, peroxidase, and phosphoglucomutase. From the isozyme analysis, esterase showed higher degree of variability, while it was observed less variability for the enzymes such as glucosephosphate isomerase, malate dehydrogenase, and phosphoglucomutase. The species P. ostreatus, whose taxon is controversial, was discriminated from P. pulmonarius, while P. florida was classified as a distinct taxon. The clustering of P. sapidus and P. spodoleucus strains appeared to be more difficult. It was found that some strains were included to another cluster based on electrophoretic banding patterns. These results show that this lack of congruence among data sets may help explain the taxonomic difficulty within the genus Pleurotus. A dendrogram of genetic similarities was presented, and applications of isozyme data to the systematics of these commercially important fungi was discussed.
Identification of Varieties by Biochemical Methods in Pleurotus spp.
Kim, Dong-Hyun ; Kong, Won-Sik ; Kim, Kyung-Soo ; Kim, Young-Ho ; You, Chang-Hyun ; Kim, Young-Bae ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 173~181
To identify genetic difference of 13 strains in three Pleurotus species, analyses of rDNA, AP-PCR and RFLP were carried out. IGRI and
regions of rDNA amplified by PCR were about 0.9 and 0.7 kb, respectively. These PCR products were digested with six restriction enzymes to analyse polymorphism. Especially, treatment of HaeIII enzyme on
regions showed specific bands in three Pleurotus sajor-caju strains. Genetic differences among three species were classified by similarity analyses based on rDNA polymorphism. Various band patterns of
were showed by AP-PCR. Identification of species and varieties in 13 Pleurotus strains was possible according to primers used in AP-PCR. In order to develop genetic markers, RFLPs using IGRI and
probes derived from ASI 2180 and 2070 were carried out on eight Pleurotus varieties. RFLP patterns using IGRI probe were more various than that of
Artificial Cultivation of Tricholoma giganteum Collected in Korea (I) - Morphological Charateristics of Fruitbody and Environmental Condition in Habitat of T. giganteum -
Kim, Han-Kyoung ; Kim, Yang-Sup ; Seok, Soon-Ja ; Kim, Gwang-Po ; Cha, Dong-Yeul ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 182~186
The result of study on the morphological feature of fruitbody, soil characteristics and enviromental condition of habitat of Tricholoma giganteum in Korea was as follows: The Pileus was
in diameter and
in thickness, indicating of yellowish white, beige, or ivory color. The lamellae were
in diameter; the stipe was
wide. The spores were
in size, ellipsoid, hyaline and the spore deposit white. The basidia were
in size. Cheiloystidia were
in size. Pileipellis were
wide and had clamp connection. Stipitipellis were
in size. Texture of soil in habitate of T. giganteum was silty loam, and organic materials content and available phosphate content in this soil were high as comparision to general soil in Korea. In the time of the mushrooms sprout, the room temperature indicated
, their humidity showed
, and the brightnesses were 328 Lux.
Formation and Characteristics of Oidia in Flammulina velutipes
Kim, Young-Ho ; Kong, Won-Sik ; Kim, Kyung-Soo ; You, Chang-Hyun ; Kim, Han-Kyoung ; Sung, Jae-Mo ; Ryu, Young-Jin ; Kim, Kwang-Ho ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 187~193
The effects of medium, incubation temperature, incubation period, pH of medium and
ondition during mycelial growth were investigated to study the factors associated with the formation of oidia in Flammulina velutipes. Oidia formation was increased when mycelial growth was poor, while oidia formation was inhibited in optimum condition of mycelial growth. Mating type of oidia was investigated to examine the effect of oidia formation on dikaryotic strain. Di-mon matings between oidia strains and original dikaryotic strain were carried out. Monokaryotic strains derived from oidia showed only one genotype. Seventy percent among Dimon mating strains showed slow mycelial growth and low yield of fruit-body, but others showed similar or high mycelial growth and yields in comparision with original dikaryon strain. One strain from di-mon mating demonstrated some differences in isozyme band pattern.
The Fruit-body Formation and Properties of Pholiota sp.
Sung, Jae-Mo ; Lee, Jae-Keun ; Park, Dong-Soo ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 194~199
Pholiota sp. were collected from the forest of Kangwon province (Mt. Samak, Mt. Odae and Kangwon National University Forest Experimentation) from the early of August to the end of October 1997. Among these fungi, Poliota adiposa was selected for culture experiment because it is suitable for edible mushroom. The Optimal temperature for the mycelial growth of Pholiota adiposa was in the range of
while that of fruit body formation was
. Brown sugar as carbon source and soybean flour as nitrogen source were good for mycelial growth in commercial liquid culture. The fruit bodies of Pholiota adiposa were artifitially produced on plastic bottle including poplar sawdust and rice bran (4:1).
Notes on Korean Strobilomycetaceae (I) - On Strobilomyces and Heimiella -
An, Yong-Hwan ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 200~210
To study the species diversity of the genera Strobilomyces and Heimiella in Korea, 52 specimens were collected from 1982 to 1996 at 25 locations including Odaesan. Collected specimens were observed and three species among them were identified and described by observing macroscopic and microscopic characters. The three species are as follows; Strobilomyces floccopus (Vahl ex Fries) Karsten, S. confusus Singer, Heimiella japonica Hongo.
Notes on Korean Strobilomycetaceae (II) - On Boletellus -
An, Yong-Hwan ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 211~229
To study the species diversity of the genus Boletellus in Korea, 56 specimens were collected from 1982 to 1996 at 20 locations including Odaesan. Collected specimens were observed and seven species among them were identified and described by observing macroscopic and microscopic characters, two of which were confirmed new to Korea: Boletellus chrysenteroides (Snell) Snell. Boletellus fallax Corner. Seven species were described here: Boletellus chrysenteroides (Snell) Snell, B. elatus Nagasawa, B. emodensis (Berk.) Singer, B. fallax Corner, B. obscurecoccineus
Singer, B. russellii (Frost) Gilbert, B. shichianus (Teng & Ling) Teng.
Notes on Korean Strobilomycetaceae (III) -On Austroboletus and the Key to Genera of the Strobilomycetaceae-
An, Yong-Hwan ; Kim, Yang-Sup ; Seok, Soon-Ja ; Yoon, Kyung-Ha ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 230~238
To study the species diversity of the genus Austroboletus in Korea, 12 specimens were collected from 1982 to 1996 at 5 locations including Odaesan. Collected specimens were observed and three species among them were identified and described by observing macroscopic and microscopic characters, one of which was confirmed new to Korea.: Austroboletus subvirens (Hongo) Wolfe. The three species are as follows; Austroboletus fusisporus (Kawam. ap. Imaze. & Hongo) Wolfe, A. gracilis (Peck) Wolfe and A. subvirens (Hongo) Wolfe and registered here with the key to Genera of the Strobilomycetaceae in Korea.
Effects of Mixed Carbon Sources on the Production of Cellulase by Trichoderma reesei
Nam, Joo-Heon ; Koo, Yoon-Mo ; Yun, Hyun-Shik ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 239~245
The feasibility of enzymatic hydrolysis of cellulosic materials is dependent on the cost of cellulase, which is strongly influenced by the selection of proper carbon source in the cellulase production medium. When solka floc was used as a carbon source for the production of cellulase by Trichoderma reesei Rut C-30, a maximum of 53.2 U/ml of CMCase activity (4.8 U/ml of FPase activity) was obtained with a concentration of 1 % of solka floc. The cellulase activity decreased to 50% in the presence of 0.5% of glucose in the medium. The production of cellulase was considerably enhanced when solka floc and wheat bran were used together as a carbon source. A medium which contained 1 % of solka floc and 3 % of wheat bran yielded highest cellulase activity: CMCase activity of 76 U/ml and FPase activity of 12.5 U/ml.
Production of Nitric Oxide in Raw 264.7 Macrophages treated with Ganoderan, the
of Ganoderma lucidum
Han, Man-Deuk ; Lee, Eun-Sook ; Kim, Young-Kweon ; Lee, June-Woo ; Jeong, Hoon ; Yoon, Kyung-Ha ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 246~255
Ganoderan (GAN), an immunomodulating
of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. This study was set up to elucidate the ability of macrophage activation of GANs. GAN-treated Raw 264.7 macrophages showed enhanced production of nitric oxide (NO). The ability of GANs to produce NO was based on differences in chemical composition of GANs obtained from the mycelium on various carbon sources and mycelial fractionation. The highest NO production was observed in CW-AS-WS polysaccharide which was extracted from the mycelial wall. GAN-treated Raw 264.7 cells gave a 2-to 5-fold (24 hr) formation of NO levels compared with those treated with medium only. Partial removal of the protein in the extracellular GAN by TCA treatment did appreciably reduce its capacity to secrete NO. The mixture effect of GAN and LPS increased the nitric oxide secretion from RAW 264.7. The cell proliferation of GAN-treated Raw 264.7 cell tines inhibited as compared with its control. Of the culture supernatant of macrophage activated by GAN, the percentage of cytotoxicity against mouse leukemia L1210 cells was slightly dependent on the amount of NO in the culture supernatants of the activated-macrophages. These results indicate that the
polysaccharides of the higher fungus activate macrophage and release nitric oxide. It also suggests that murine macrophages possess certain receptors for
glucans and play a critical role of
tumor killing mechanism.
The 18s rDNA Sequences of the Basidiocarps of Tricholoma matsutake in Korea
Lee, Sang-Sun ; Hong, Sung-Woon ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 256~264
The 18S rDNA sequences of Tricholoma matsutake (TM=T. caligatum var. nauseoum) collected in Korea were analyzed for the ectomycorrhizal fungi in the roots of Pinus densiflora. The 514 base pairs of rDNA region were synthesized by UF-5 and UR-6 primers, and double checked in the base pair. The sequence of four strains synthesized were all identical in this work, but different from those done by the previous workers. The basidiocarps collected in this work. were identified to T. matstake after searching the 18s rDNA by the BLAST in NCBI. Only several base pairs of 18S rDNA analyzed from other related basidiocarps were different from our analyses of 18S rDNA. The dendrogram were made based on the sequences of the 514 bp 18S rDNA by CLUSTAL-X alignment program. The groupings of the species at the level of genus in the dendrogram were well constructed.
Optimization for Solid Culture of Phellinus sp. by Response Surface Methodology
Kang, Tae-Su ; Kang, An-Seok ; Sohn, Hyung-Rac ; Kang, Mi-Sun ; Lim, Yaung-Iee ; Lee, Shin-Young ; Jung, Sung-Mo ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 265~274
This study was carried out to obtain the basic data for an artificial cultivation of Phellinus sp.. The optimum conditions for the mycelial growth on the different sawdusts (Quercus aliena, Morns alba and Alnus japonica) substrate of an isolated Phellinus sp. were optimized by response surface methodology. The ratio of rice bran addition to sawdust and the suitable moisture content for the mycelial growth in the all sawdust media were about 30% (w/w) and
(w/v), respectively. The initial pHs for the mycelial growth of Quercus aliena and Morns alba were in the range of
, whereas Alnus japonica was obtained at pH 6. The optimum temperature for the mycelial growth was about
, depending on the different kinds of wood substrates. From the response surface analysis, the values of independent variables of Quercus aliena at stationary points were determined to be 31.01 % (w/w) of rice bran, pH of 5.31 and 69.03% (w/v) of moisture content, and the expected value of mycelial growth was about 8.32 cm. Both the ratio of rice bran addition to sawdust
and moisture content
were effective to the mycelial growth. In the case of Morns alba, the ratio of rice bran addition to sawdust, initial pH and moisture content at the stationary points were 28.77% (w/w), 5.28 and 69.8 (w/v),respectively, and the expected mycelial growth of 7.60 cm was obtained. Stationary points for the mycelial growth in the sawdust media of Alnus japonica were 28.74% (w/w) of rice bran, pH of 6. 04 and 66.96% (w/v) of moisture content, and the expected values of mycelial growth was about 5.38 cm. Based on the above results, there was correlations between the mycelial growth and independent variables, and the effect of rice bran
and initial pH
for the mycelial growth were higher than the moisture content
. The optimum species of sawdust media for the my celial growth of Phellinus sp. was in the order of Quercus aliena > Morns alba > Alnus japonica.
Protoplasts Isolation and Reversion of Fomitella fraxinea
Kim, Kyung-Soo ; You, Chang-Hyun ; Kong, Won-Sik ; Kim, Young-Ho ; Cha, Dong-Yeul ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 275~280
Factors affecting protoplasts isolation and regeneration of Fomitella fraxinea were investigated. Lytic enzyme mixture of Novozym 234, Cellulase onozuka R-10 and
was found to be the best for the protoplasts isolation. Osmotic stabilizer of 0.6 M sucrose was observed as the best for protoplasts isolation. The highest number of protoplasts was obtained from the F. fraxinea mycelium with lytic enzyme mixture and osmotic stabilizer that had been cultured for 3 hours. The highest regeneration rate of 0.02 % was achieved when the 0.6 M sorbitol was employed as osmotic stabilizer.
Juice Clarification with the Use of Polygalacturonase Produced by Ganoderma lucidum
Yoon, Sook ; Kim, Myung-Kon ; Hong, Jai-Sik ; Park, Il-Woong ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 281~286
Ganoderma lucidum produced the potent pectolytic enzymes for clarifying cloudy fruit juice. Among the purified polygalacturonases (endo- and exo-polygalacturonase), endo-polygalacturonase had a good effect on juice clarification. The optimum temperature and concentration of endo-polygalacturonase for the juice clarification were
and 4 unit/5 ml juice, respectively. The apple juice was almost completely clarified at
for 60 min. It was suggested that culture filtrate of Ganoderma lucidum or it's ammonium sulfate fraction should be used as a good source of pectolytic enzyme for juice clarification.
In vitro Formation of Cochliobolus nisikadoi, the Perfect State of Bipolaris coicis
Kim, Sung-Kee ; Kim, Ki-Woo ; Park, Eun-Woo ; Kang, Wee-Soo ; Yang, Jang-Souck ;
The Korean Journal of Mycology, volume 26, issue 2, 1998, Pages 287~292
The perfect state of Bipolaris coicis, causing leaf blight of Job's tears, was in duced under in vitro conditions. Eighty nine isolates of the fungus were collected from 17 locations in Korea from 1994 to 1996. They were crossed on Sach's nutrient agar, on which a piece of rice straw was placed, and incubated at
. Pseudothecia were produced only by certain combinations of compatible isolates. Although pseudothecia were usually produced on rice straw two weeks after incubation, asci and ascospores were observed only in a few pseudothecia examined. The pseudothecia were black and globose with protruding ostiolar beaks. The locules were filled with a mass of hyaline and filamentous pseudoparaphyses. Asci were cylindrical to clavate and straight or slightly curved. The ascus wall was bitunicate with short stipes. Ascospores were filiform, hyaline, and arranged parallel to slightly coiled in the asci, measuring
. Perfect state of the fungus was identified as Cochliobolus nisikadoi (Tsuda, Ueyama & Nishihara Alcorn), based on the morphological characteristics.