Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 27, Issue 6 - Dec 1999
Volume 27, Issue 5 - Oct 1999
Volume 27, Issue 4 - Aug 1999
Volume 27, Issue 3 - Jun 1999
Volume 27, Issue 2 - Apr 1999
Volume 27, Issue 1 - Feb 1999
Selecting the target year
Growth Condition of Liquid Culture by Pleurotus ostreatus
Sung, Jae-Mo ; Moon, Hee-Woo ; Park, Dong-Soo ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 1~9
For the practical using of liquid spawn we carried out selection test of nutrient sources, cultural methods and cultural apparatus for liquid spawn production of oyster mushroom(ASTI 2001, ASTI 2018, ASTI 2072, ASTI 2016, ASTI 2070, ASTI 2180, ASTI 2183, ASTI 2042). The optimal temperature and pH range for mycelial growth of Pleurotus species were
and 5.5 to 6.5, respectively. The effect of carbon sources, nitrogen sources and mineral salts on the mycelial growth was studied using petridish culture. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of Pleurotus species, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth. For the mycelial growth of the 8 oyster mushroom stains, soybean flour was superior to the other kinds of nitrogen sources. On the other hand, addition of mineral salts did not affect, and even poor under certain mineral salts, the mycelial growth of the 8 oyster mushroom stains. The brown sugar selected out the carbon source of the agricultural medium. Also the soybean flour selected out the nitrogen source of agricultural medium. In the medium selection, we selected out agricultural optimum medium composed of brown sugar 3%, soybean flour 0.3%, potassium phosphate 0.05%, magnesium sulfate 0.05%. Under the 250 ml erlenmeyer culture, the effects of such factors as the inoculum rate, the working volume, cultural method and flask shapes on the mycelial growth were examined. The optimal inoculum rate and working volume on the mycelial growth of oyster mushroom was 2 mycelial disk (diameter 6 mm) and 100 ml, respectively. The shake flask culture was enhanced the mycelial growth than at the erlenmeyer flask. Pulp form growth of mycelium in the erlenmeyer flask culture was obtained in the culture with glass rod of length 50 mm, diameter 10 mm.
A Short Composting Method by the Single Phase Composter for the Production of Oyster Mushroom
Lee, Ho-Yong ; Shin, Chang-Yup ; Lee, Young-Keun ; Chang, Hwa-Hyoung ; Min, Bong-Hee ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 10~14
A single phase composter was constructed by modifying the conventional mixer of sawdust for the cultivation of oyster mushroom Pleurotus ostreatus. The machine was designed on the basis of 3-phase-1 system which was controlled in prewetting, pasteurization and fermentation processes. In composting 200 kg of straw and cotton waste in the machine, it took 20 minutes in prewetting step and also to hours at
in pasteurization process. Postfermentation by aerothermophiles was completed by treating the compost at
for 48 hours which was shorten 24 hours from the conventional method. In the postfermentation at high temperature, forced aeration and/or vigorous mixing process(es) played a great role in the improvement of spawn quality. The growth of mycelium of oyster mushroom was excellent in the culture combinated with 3 parts of surface inoculation and 7 parts of mechanical mixing.
Production of Fruiting Body Using Cultures of Entomopathogenic Fungal Species
Sung, Jae-Mo ; Choi, Young-Sang ; Lee, Hyun-Kyung ; Kim, Sang-Hee ; Kim, Yong-Ook ; Sung, Gi-Ho ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 15~19
One hundred and six Cordyceps cultures including five cultures of Paecilomyces tenuipes were used for production of artificial fruiting body. In the test of artificial fruiting body formation, no fruiting bodies were induced on media containing PDA and ground silkworm pupae with liquid nitrogen. The best fruiting body formation was showed on media which mixed at the ratio of 1 unsticky rice to 3.5 water. But fruiting bodies formed on media mixed at the ratio 1 unpolished rice to 2.5 water. Optimal temperature in inducing artificial fruiting body was at
. Twenty seven isolates were selected as good cultures for production of artificial fruiting body. Maturation of fruiting bodies incubated on rice grain media was completed for about 50 to 65 days.
The Specific Probes Confirming the Genomic DNA of Tricholoma matsutake in Korea
Lee, Sang-Sun ; Hong, Sung-Woon ; Chung, Hung-Chae ; Sung, Chang-Kun ; Kim, Jae-Hun ; Ka, Kang-Hyeon ; Kim, Hyun-Joong ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 20~26
The specific DNA band appeared in PCR-RAPD analysis using OPO-2 primer was a very important for the researching Korean pine-mushrooms, Tricholoma matsutake. This DNA band, sequenced to be the 770 base pairs, existed as only a single copy in the whole genomic DNA's of Korean pine-mushrooms. However, this band was not presenting from the PCR-RAPD bands of other ectomycorrhyzal fungi reacted with the OPO-2 primer or the dot blots. Also, this DNA sequence was not matched with those of the other genes known by NCBI and had low homology together with sequence of other proteins compared. Those results suggested that the specific DNA band can be used as probe for identification of T. matsutake and might be related to the informations rather than the gene for the proteins with analysis of protein sequence translated from the DNA sequence.
Genetic Relationships of Coprinus spp. on the Basis of Sequences in ITS II Region
Park, Dong-Suk ; Go, Seung-Joo ; Kim, Yang-Sup ; Seok, Soon-Ja ; Song, Jae-Kyeong ; Yeo, Yun-Soo ; Ryu, Jin-Chang ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 27~31
The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Coprinus spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. lagopus, C. cinereus, C. rhizophorus, C. flocculosus, C. radians, and C. echinosporus were compared. The spacer regions of them were
nucleotide in length and partially contained 5.8S and 25S. The reciprocal homologies of each ITS II sequence among these strains were in the range of
. According to the analysis of ITS II sequences, Coprinus spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, C. niveus, and C. atramentarius. Cluster II comprised C. micaceus, C. flocculosus, C. radians, and C. disseminatus. On the other hand C. comatus is in Cluster III even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that Coprinus comatus, which was considered as a type species of the genus Coprinus in morphological classification, gives a doubt of monophyletic evolution and is assumed to be paraphyletic or polyphyletic.
Pathological Characteristics of Saprolegnia sp. to Snakehead and Effect of Essential Oils to the Growth of the Water Mold
Lee, Keun-Kwang ; Kim, Young-Gill ; Lee, Min-Woong ; Lee, Hyung-Hoan ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 32~38
A Saprolegnia sp. was isolated from cultured snakehead, Channa argus, and its physiological characteristics were investigated. The optimum temperature, pH and NaCl concentration for mycelial growth of Saprolegnia sp. were
, 6.0 and 0%, respectively. The mycelial growth was increased with the addition of 10 mM phosphate and 10mg/L casamino acid. The essential oils extracted from three plants, Artemisia princeps var. orientalis, Thuja orientalis and Chamaecyparis obtusa have been tested to know whether they inhibit the growth of Saprolegnia sp. at six different oil concentrations(10, 100, 500, 1,000, 1,500 and 2,000 ppm). Essential oil from A. princeps var. orientalis began to inhibite the mycelial growth of Saprolegnia sp. at the concentration more than 10ppm. Using other essential oils from T. orientalis and C. obtusa, those initially inhibited the mycelial growth of Saprolegnia sp. at the concentration over 10 ppm and complate inhibition of mycelial growth was observed at over 500 ppm. The histopathological features of Snakehead infected by Saprolegnia sp. were studied. A club shape of gill lamella epithelial cells was observed in the gill. The mycelial cells were penetrated into muscular tissue, and the accumulation of the ceroid was observed in the liver, spleen and kideny tissue in common. The necrosis of tubular epithelial cells was seen in the liver tissue, parenchymal tissue in the spleen and tubular epithelial cells in the kidney.
Phylogenetic Study of Ganoderma spp. Based on the DNA Sequences in ITS II Region
Park, Dong-Suk ; Go, Seung-Joo ; Ryu, Jin-Chang ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 39~43
The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Ganoderma spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 9 species including Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum and Inonotus xeranticus as an out-group were compared. The spacer regions of them were
nucleotides in length and contained partial sequences of 5.8S and 25S gene. The reciprocal homologies of each ITS II sequence of the species were in the range of
except outgroup species, I. xeranticus. According to the analysis of ITS II sequences, Ganoderma spp. constructed 5 clusters. Ganoderma lucidum isolates were to be divided into two groups. One group was consisted of isolates from South Korea. The other group comprised isolates from UK. G. lucidum isolates belonging to the group I were closely related with G. tsugae. These results suggested that G. lucidum from Korea should be G. tsugae, otherwise G. tsugae was to be synonym of G. lucidum.
Taxonomic Studies on Cercospora and Allied Genera in Korea (V)
Kim, Jeong-Dong ; Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 44~53
This paper is the fifth contribution towards taxonomic studies on Cercospora and allied genera, and contains ten species of Korean cercosporoid fungi; viz., Cercospora adusta, C. chrysanthemi, C. ludwigiana, C. zebrina, Passalora depressa, Pseudocercospora destructiva, P. lonicericola, P. nojimai, Pseudocercosporella inconspicua, and Ramularia major. Morphological characteristics of taxonomic value are described and illustrated for these species to contribute towards a mycological monograph of Korean cercosporoid fungi.
Taxonomic Studies on Cercospora and Allied Genera in Korea (VI)
Kim, Jeong-Dong ; Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 54~62
This paper is the sixth contribution towards taxonomic studies on Cercospora and allied genera, and contains ten species of Korean cercosporoid fungi; viz., Cercospora apii, C. aristolochiae-contortae, C. violae, Neoramularia bidentis, Phaeoramularia weigelicola, Pseudocercospora cantuariensis, P. cydoniae, P. profusa, Pseudocercosporella sublineolata, and Ramularia plantaginis. Morphological characteristics of taxonomic value are described and illustrated for these species to contribute towards a mycological monograph of Korean cercosporoid fungi.
Two Entomopathogenic Conidiobolus Species First Observed on the Aphids in Korea
Yoon, Cheol-Sik ; Sung, Gi-Ho ; Park, Hyun-Soo ; Yoo, Jai-Ki ; Lee, Jeang-Oon ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 63~65
Conidiobolus obscurus and C. thromboides (Zygomycetes: Entomophthorales), aphid-attacking fungi, were found on the Dactynotus species (Homoptera: Aphididae) in June 1998 for the first time in Korea. They produce globose primary conidia typical to the genus Conidiobolus but their dimensions are clearly distinguished. Conidiobolus thromboides produces rhizoids and conidiophores with cylindrical constriction at their apices but C. obscurus does not form rhizoids or constricted conidiophores. Resting spores were not found in our specimens of both species, but their vegetative structures observed readily allowed identification.
First Record of the Entomopathogenic Fungus Neozygites fresenii on the Aphid in Korea
Yoon, Cheol-Sik ; Lee, Min-Ho ; Lee, Seung-Hwan ; Yoo, Jai-Ki ; Lee, Jeang-Oon ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 66~67
Neozygites fresenii (Zygomycetes: Entomophthorales), aphid-attacking fungus, was found in June 1998 for the first time in Korea. The fungus produces the globose primary conidia with a truncate papillar and two types of the secondary conidiophores and conidia. Resting spores were not found in our specimens, but the fungal structures observed clearly distinguish the fungus from other aphid-attacking fungi, allowing inclusion in the species N. fresenii.
Taxonomic study on Korean Aphyllophorales (IV) - on some unrecorded wood-rotting fungi -
Lim, Young-Woon ; Hong, Soon-Gyu ; Jung, Hack-Sung ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 68~72
Wood-rotting fungi of the Aphyllophorales were collected through field trips to mountain areas of the country from January to December of 1997. Through the observation and identification of specimens, two genera, Meruliopsis (type species=M. taxicola) and Pseudomerulius (type species=P. aureus), and four species, Phanerochaete calotricha, Phanerochaete chrysorhiza, Meruliopsis corium, and Pseudomerulius aureus were confirmed as new wood-rotting fungi to Korea and are registered here with descriptions.
Taxonomical Characteristics of Tropical Species of Xylaria Collected from Taiwan
Lee, Yang-Soo ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 73~75
Three species of the genus Xylaria are recognized on Taiwan materials: X. allantoidea (Berk.) Fr. and X. obovata (Berk.) Fr.; including one new record X. curta Fr., having
ascospores, in Taiwan. The collection of Xylaria allantoidea from Taiwan is compared to other collections in morphological characters. The species are described from the host and in culture by a bright microscope and a scanning electron microscope.
Structural Analysis of the Antitumor Active Exo-polysaccharide Produced by Submerged Cultivation of Ganoderma lucidum Mycelium
Lee, Shin-Young ; Kang, Tae-Su ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 76~81
Exo-polysaccharide obtained from the submerged cultivation of Ganoderma lucidum mycelium was fractionated. The structural analysis of the acidic exo-polysaccharide fraction (BWS-DA-GI), showing high antitumor activity, was carried out and compared to the mycelial acidic fraction (MWS-DA-GI). The major sugar constituents of the fraction of BWS-DA-GI were glucose, galactose and mannose in the molar ratio of 2.5 : 2.1 : 2.5. The minor components in this fraction were xylose and fucose. While the major sugar constituents of the mycelial acidic fraction of MWS-DA-GI were galactose, fucose, mannose and glucose. The trace components in this fraction was xylose. From the results of periodate oxidation, Smith degradation, affinity chromatography and methylation analysis, the chemical structures of the two fractions, BWS-DA-GI and MWS-DA-GI were both determined as
glucans. It was also estimated that BWS-DA-GI had a
glucosidic linkage and MWS DA-GI had
glucosidic linkages. The molecular weights of these fractions, MWS-DA-GI and MWSDA-GI were estimated as
Hepatoprotective Effect of Exo-polysaccharide Produced from Submerged Mycelial Culture of Ganoderma lucidum WK-003 by Using Industrial Grade Medium
Yang, Byung-Keun ; Jeon, Yong-Jae ; Jeong, Sang-Chul ; Kim, Dong-Hyun ; Ha, Ji-Young ; Yun, Jong-Won ; Shon, Dong-Hwan ; Go, Geon-Il ; Song, Chi-Hyun ;
The Korean Journal of Mycology, volume 27, issue 1, 1999, Pages 82~86
The production of hepatoprotective exo-polysaccharide by using synthetic and industrial grade media of the submerged mycelial culture of Ganoderma lucidum WK-003 was compared. The optimum concentrations of molasses and corn steep liquor (industrial grade) for the production of exo-polysaccharide were 5% and 2.5%, respectively. The productions of the exo-polysaccharide by using a 5l jar fermenter with industrial grade medium and synthetic medium were 11.2 g D.W./l and 7.2g D.W./l, respectively. Glutamic pyruvic transaminase (GPT) activities in the serum of intoxicated Sprague-Dawley rats by oral administration of the exo-polysaccharide produced from the industrial grade and synthetic media for 4 consecutive days were decreased from 704 IU/L to 356IU/L and 704IU/L to 349IU/L, respectively.