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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 27, Issue 6 - Dec 1999
Volume 27, Issue 5 - Oct 1999
Volume 27, Issue 4 - Aug 1999
Volume 27, Issue 3 - Jun 1999
Volume 27, Issue 2 - Apr 1999
Volume 27, Issue 1 - Feb 1999
Selecting the target year
The Production of Artificial Fruiting Body of Paecilomyces japonica
Choi, In-Young ; Choi, Joung-Sik ; Lee, Wang-Hyu ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 87~93
This study was conducted to investigate the morphological characteristics and cultural conditions for artificial fruiting body(synnemata) production of Paecilomyces japonica. In the morphological characteristics of P. japonica, the size of it's conidia was ranged from
. The artificial fruiting body showed yellow in color, shape was confirmed ellipsoidal or obovoid type, and the length was
. The mycelial growth on the PDA medium treated with pH7, at
was superior to that of other treatments. The formation period of an artificial fruiting body of P. japonica treated with polypropylene and glass bottle culture was 30 days and 50 days, respectively. The length and number of fruiting body was longer and higher in the polypropylene bottle culture than those of the glass bottle culture. As the results, the artificial fruiting body production in the polypropylene bottle increased 1.2g per bottle compared to that of the glass bottle. It also increased in
illumination, whereas the elongation of synnemata, pinheading and fruiting body growth were inhibited by continuous use of 900 lx illumination. The results of these experiment indicated that fruiting body formation seemed to be lower as the light intensity increased. The fruiting body formation was also dependent on the light color. There was a higher incidence in red color light and fluorescent light treatment than that of incandescent and blue color light. The fruiting body of the naked barley medium had so much better growth compared to other media that it would be able to use for it's production. The growth of fruiting body was affected by
concentration. It increased after putting the lid on the bottle.
Studies on the Celluloytic Enzymes Produced by Stropharia rugosoannulata in Synthetic Medium
Yoo, Kwan-Hee ; Chang, Hyung-Soo ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 94~99
For the purpose of utilizing cellulose resources by cellulolytic enzymes of Stropharia rugosoannulata, it's cultural conditions for the prodution of cellulolytic enzymes in synthetic media were investigated. The optimum pH for the production of Avicelase and
was pH 5.0, while that of CMCase was pH 4.0. The optimum temperature for the production of Avicelase, CMCase and
. Among the carbon sources, xylose was good for the production of CMCase and
, but maltose was good for the production of Avicelase. The optimum concentration of the carbon sources for the production of CMCase, Avicelase and
was 1.0, 0.8 and 1.1%, respectively. As inorganic nitrogen sources,
was good for the production of all the three cellulolytic enzymes. The optimum concentration of
for the production of CMCase was 0.3% while that of Avicelase and
was 0.4%. As organic nitrogen sources, malt extract was good for the production of all the three cellulolytic enzymes. The optimum concentration of organic nitrogen for the production of
was 1.3% while that of CMCase and Avicelase was 1.0%. As the mineral sources,
good for the was good for the production of all the three cellulolytic enzymes. The optimum concentration of
for the production of all the three enzymes was 0.35%.
Characteristics and Purification of Polysaccharide Produced from Agrocybe cylindracea
Kim, Seon-Hee ; Jung, In-Chang ; Kim, So-Yeun ; Lee, Jong-Suk ; Cho, Hyen-Jae ; Lee, Hang-Woo ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 100~106
The polysaccharides, intracellular and extracellular, extracted from the liquid culture of the Agrocybe cylindracea were purified and characterized. The mycellial cellular productivity of Agrocybe cylindracea was proved to be almost 2 folds in the shaking culture compared to the standing culture. These polysaccharides were purified by the DEAE-cellulose ion exchange chromatography and the Sepharose 2B size exclusive gel filteration. The two purified fractions of extracellular polysaccharides, ACEPDG and ACEPAG, contained 75.8% and 65.4% total sugar respectively. The total sugar content of ACIPDG and ACIPAG, the two purified fractions of intracellular polysaccharides, were 89.2% and 54.2% respectively. The molecular weights range of all the substances were estimated to be above 100,000, from 300KDa of ACEPDG to 600 KDa of ACIPAG. The results of sugar analysis by HPLC showed that the sugar part of ACEPDG was consisted of glucose and inositol. The ACIPDG, ACEPAG and ACIPAG contained three kinds of monosaccharides, glucose, fructose and inositol.
Studies on the Effect of Vinyl Mulching on Pleurotus ostreatus Cultivation - Quality and Productivity of Crop (I) -
Oh, Se-Jong ; Chun, Chang-Sung ; Park, Jung-Sik ; Kim, Hee-Kyu ; Fermor, T.R. ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 107~111
Vinyl mulching was used on Oyster mushroom beds in an attempt to control bacterial diseases of Pleurotus ostreatus. Beds were covered with perforated, transparent vinyl sheets (0.03 mm thick, 180 cm wide); holes (10 cm diameter) were 10 cm apart. There were
. Vinyl mulching made management of the crop easier. Discrete mushroom flushes formed with high quality bunches of mushrooms formed through the holes; this made picking quicker and made more efficient use of labor. Total yield of mushrooms increased by 5.7% to
. Mushroom mycelium under vinyl remained healthy and white; whereas mycelium grown using conventional methods changed from white to yellow-brown during the cropping cycle. The mean weight of a mushroom bunch from vinyl mulched beds was 283 g (33 fruitbodies) compared to 117 g (15 fruitbodies) obtained using conventional growing methods.
Electrophoretic Karyotypes of Fusarium oxysporum f. sp. lycopersici
Kim, Young-Tae ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 112~118
Strains of Fusarium oxysporum f. sp. lycopersici isolated from Korea, Japan and U.S.A. were used for electrophoretic karyotype (EK) analysis. Chromosome separations on FastLane agarose gels (FMC BioProducts, Rockland, ME), called pulsed field gel electrophoresis (PFGE), were performed by CHEF-DRII apparatus (Bio-Rad Laboratories, Melville, NY) using TAE as a running buffer. To obtain optimal condition for separation of chromosome sized DNAs, variable running conditions such as field strengths, swithching intervals, and running time were applied in CHEF gel electrophoresis. We were able to resolve 9 to 11 chromosome sized DNAs ranging in size from 0.76 to 6.41 Mb in isolates from Korea and estimate that the total genome size was ranging from 35.29 to 38.92 Mb. Distinct differences in length range and genome size exist among isolates from different countries. Isolates from Japan and U.S.A. were resolved 9 to 11 chromosome sized DNAs ranging in size from 1.24 to 6.85 Mb and estimated that the total genome size was ranging from 35.32 to 43.87 Mb. Isolates from variable provinces in Korea had the same or similar chromosomal polymorphism and showed different chromosomal DNA patterns compared to isolates from the other countries.
Genetic Diversity of Korean Isolates of Pseudomonas tolaasii and WLRO (White Line Reacting Organism) using BOX-, REP-, and ERIC-PCR
Chee, Hee-Youn ; Oh, Se-Jong ; Lincoln, S.P. ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 119~123
Genetic diversity of Korean isolates of Pseudomonas tolaasii and WLRO (White line reacting organism) was assessed using BOX-, REP-, and ERIC-PCR analysis. P. tolaasii showed nearly identical band patterns among isolates, whereas considerable DNA polymorphism was found among isolates of WLRO. On the basis of dendogram, WLRO is characterized as a complex group with high degree of genetic differentiation. Genetic relatedness based on repetitive DNA regions was low between P. tolaasii and WLRO isolates.
Phylogenetic Analysis of the Genus Phellinus by Comparing the Sequences of Internal Transcribed Spacers and 5.8S Ribosomal DNA
Chung, Ji-Won ; Kim, Gi-Young ; Ha, Myung-Gui ; Lee, Tae-Ho ; Lee, Jae-Dong ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 124~131
This study was carried out to identify the phylogenetic relationship among Phellinus species by comparing the DNA sequences of the 5.8S ribosomal DNA (rDNA) and the internal transcribed spacers (ITSs), ITS1 and ITS2 regions. Two primers from the 3' end of 18S rDNA and the 5' end of 28S rDNA sequences were chosen to amplify the specific ITS regions of Phellinus spp. Phellinus strains used in the study were divided into four clusters by the phylogenetic tree based on the amplified regions of ITS and 5.8S rDNA sequences. The first cluster consist of Phellinus hartigii IMSNU 32041 and Phellinus robustus IMSNU 32068, and the second cluster consists of Phellinus linteus strains and Phellinus weirianus IMSNU 32021. Phellinus laevigatus KCTC 6229, KCTC 6230 and Phellinus igniarius KCTC 6227, KCTC 6228 belong to the third cluster. Finally, Phellinus chrysoloma KCTC 6225 and Phellinus chrysoloma KCTC 6226 are the fourth cluster. In the second cluster the differentiation between Phellinus linteus strains and Phellinus weirianus species were not possible by the comparison of the ITS sequences. These results revealed that Phellinus linteus and Phellinus weirianus cannot be established the concept of species level only by the ITS sequences. Therefore, both physiological and molecular biological methods as well as the sequences of type strains are necessary to classify the strains of these two species accurately. The comparison of the ITS sequences of four Phellinus species indicated that the sequences of the ITS1 generally are more divergent than those of the ITS2. Although the ITS sequences are varied in some species, the conserved regions in both ITS1 and ITS2 are useful tool to differentiate the species. Phellinus linteus and related species have their specific sequences in the ITS1 compared to the other species.
Detection of Pseudomonas tolaasii Causing Brown Blotch Disease of Mushroom with Species-specific DNA Probe
Kwon, Soon-Wo ; Go, Seung-Joo ; Cheun, Meung-Sook ; Kang, Hee-Wan ; Oh, Se-Jong ; Chang, Who-Bong ; Ryu, Jin-Chang ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 132~137
This study was carried out to develop the molecular marker for the detection of Pseudomonas tolaasii, a causative agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). When several primers designed from repetitive sequences and pectin lyase genes of bacteria were used to produce DNA polymorphism from different Pseudomonas spp. isolated from edible mushrooms, PEU1 primer derived from pectin lyase gene produced polymorphic bands differentiating P. tolaasii strains from other Pseudomonas species. Two bands, 1.0kb and 0.4kb, found commonly in 6 isolates of P. tolaasii were cloned into pGEM-T vector which were designated as pPTOP1 and pPTOP2, respectively, to use as probe. The 0.4 kb insert of pPTOP2 hybridized to only 6 isolates of P. tolaasii, but did not to the other Pseudomonas species. As few as
colony forming unit (cfu) of P. tolaasii could be detected by dot blot hybridization with the cloned 0.4kb DNA in pPTOP2.
Taxonomic Studies on Cercospora and Allied Genera in Korea (VII)
Kim, Jeong-Dong ; Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 138~146
This paper is the seventh contribution towards taxonomic studies on Cercospora and allied genera, and contains ten species of Korean cercosporoid fungi; viz., Cercospora arcti-ambrosiae, C. cichorii, C. kikuchii, C. subhyalina, Neoramularia koreana, Pseudocercospora ligustri, P. oenotherae, P. rubi, P. zelkowae, and Ramularia archangelicae. Morphological characteristics of taxonomic value are described and illustrated for these species to contribute towards a mycological monograph of Korean cercosporoid fungi.
Taxonomic Studies on Cercospora and Allied Genera in Korea (VIII)
Kim, Jeong-Dong ; Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 147~157
This paper is the eighth contribution towards taxonomic studies on Cercospora and allied genera, and contains ten species of Korean cercosporoid fungi; viz., Cercospora brassicicola, C. flagellaris, C. sesami, C. zinniae, Passalora amurensis, Pseudocercospora balsaminae, P. humuli, P. puderi, P. salicina, and Stenella dianthi. Morphological characteristics of taxonomic value are described and illustrated for these species to contribute towards a mycological monograph of Korean cercosporoid fungi.
New Records of Powdery Mildews from Ornamental Trees in Korea (I)
Shin, Hyeon-Dong ; Yang, Sung-Il ; Lee, Sang-Hyun ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 158~163
Powdery mildew diseases previously unrecorded in Korea were noticed on four species of ornamental trees. Field observations on each disease were described and the causal fungi were identified. Powdery mildew of Symplocos chinensis for. pilosa was found to occur mildly, especially on plants growing in shade. The causal fungus was identified as Microsphaera nomurae U. Braun. Cornus florida was found to be infected with Microsphaera pulchra Cooke & Peck and supposed to be epidemic in nursery. Powdery mildew of Malus baccata occurred on young leaves and herbaceous stems in nursery. The causal fungus was classified as Podosphaera leucotricha (Ellis & Everh.) Salmon. Powdery mildew of Ulmus macrocarpa was found to occur in autumn and severe on young shoots. Uncinula kenjiana Homma was identified as the causal fungus.
Screening of Chemicals on Bacterial Brown Blotch Caused by Pseudomonas tolaasii on Flammulina velutipes
Lee, Hyun-Uk ; Kim, Tae-Sung ; Park, Hyean-Cheal ; Song, Keun-Woo ; Shin, Won-Kyo ; Moon, Byung-Ju ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 164~169
This study was carried out in an attempt to select chemicals such as zeolite granules, chitosan, wood-vinegar,
, NaOCl, against bacterial brown blotch caused by Pseudomonas tolaasii on Flammulina velutipes in laboratory and mushroom unit. Results obtained using these chemicals were summarized. Inhibitory effect on
and wood-vinegar with 0.5% concentration was shown to caual pathogen, P. tolaasii, by slight damage on the mycelial growth of F. velutipes. Those materials were recognized as promising one for control of bacterial brown bloth on F. velutipes. Disease incidence in control was the highest value as 43.8%; whereas chemical treatment was estimated as 14.6% with 0.5% of
. Disease incidence was inhibited about 20% in chemical treatment with 0.5% of wood-vinegar, 1.0% of
, and 26.1% with 1.0% of wood-vinegar. For the inhibition effects of chemical concentrations, it was effective at the low concentration which was 0.5% rather than that of 1.0%. Quality of mushrooms was significantly improved, and yields was also increased by 30% in the chemical treatment. In case of
treatment at 1.0% concentration, the yields was increased by 35.6% showed the highest value among tested chemicals. However, the overall effects including disease incidence, quality and yields suggests that
is relatively more effective than wood-vinegar, and the optimal concentration controlling the disease was
and 0.5% with wood-vinegar.
Chemical Features and Purification of Immunostimulating Polysaccharides from the Fruit Bodies of Agaricus blazei
Cho, Soo-Muk ; Park, Jeong-Sik ; Kim, Kwang-Po ; Cha, Dong-Yeol ; Kim, Hwan-Mook ; Yoo, Ick-Dong ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 170~174
Water-soluble polysaccharides from the fruiting bodies of Agaricus blazei Murill were extracted with 0.9% sodium chloride and hot water, successively. The purified polysaccharides showed a potent immunostimulating activity. Eight major polysaccharides, which were named from AG-l to AG-8, were fractionated and purified by ethanol precipitation, ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. These polysaccharides were identified to be homogeneous by analysis of HPLC. Three major active polysaccharides (AG-2, -3, and -6) showed relatively strong immunostimulating activity. AG-2 and -3 were composed of glucose, galactose and mannose in the molar ratios of 74.0:15.3:10.7 and 63.6:17.6:12.7, respectively. AG-6 was composed of glucose and ribose in the molar ratios of 81.4:12.6.
Antiviral Effect of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons Against Vesicular Stomatitis Virus (New Jersey Serotype)
Rym, Kyo-Hwan ; Eo, Seong-Kug ; Kim, Young-So ; Lim, Jai-Yun ; Han, Seong-Sun ;
The Korean Journal of Mycology, volume 27, issue 2, 1999, Pages 175~179
In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [New Jersey serotype, VSV(NJ)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of VSV(NJ). EA caused a concentration-dependent reduction in the plaque formation of VSV(NJ) with 50% effective concentration
of 2.10 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha showed more potent effect with CI values of
for 50%, 70% and 90% effective levels than that with INF gamma with CI values of