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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 27, Issue 6 - Dec 1999
Volume 27, Issue 5 - Oct 1999
Volume 27, Issue 4 - Aug 1999
Volume 27, Issue 3 - Jun 1999
Volume 27, Issue 2 - Apr 1999
Volume 27, Issue 1 - Feb 1999
Selecting the target year
The Condition of Production of Artificial Fruiting Body of Cordyceps militaris
Choi, In-Young ; Choi, Joung-Sik ; Lee, Wang-Hyu ; Yu, Young-Jin ; Joung, Gi-Tae ; Ju, In-Ok ; Choi, Young-Keun ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 243~248
To compare the morphological and cultural characteristics of Cordyceps militaris, forming conditions of artificial fruiting body production were investigated in this experiment. The stroma shapes of artificial fruiting body were confirmed to club and/or stick. However, both shapes were same color as a orange. Perithecia of stroma was semi-egg shape and was ranged from
and it's asci was narrowly cylindric. Ascospore of perithecia was belong to filiform and multiseptate. The conidia of C. militaris was centurally grown and shaped with globose, long clavate type, floccose, centrally raised during anamorph stage. When it was cultured between glass and PP bottle, production of artificial fruiting body, pinheading ratio and total yield were higher in PP bottle. The optimum illumination was 1,000 lx for the those production. The culture medium of barley, wheat and hulled rice showed higher artificial fruiting body compared to that of silkworm. Pinheading and yield of it's isolates was decreased at more than three subsequent transculture.
Effect of Different Cultivation Methods on Yield of Hericium erinaceus
Chang, Hyun-You ; Roh, Mun-Gi ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 249~251
The yields and the biological efficiency of Hericium erinaceus in the case of the bottle cultivation was 356 g/850 ml, 147.8% and in pot cultivation was 810 g/2,500 ml, 114.3% respectively. On the primordial formation the case of no removing inoculation spawn was well introduced, and the mushroom's yield and biological efficiency was to be high in the case of the bottle's cap was shut. The results in the use of the logs for Hericeum erinaceus's cultural media were to be fine in oak, alder, poplar, black locust in order. And in oak, the yield of the mushrooms were
biological efficiency was 17.3%, the period requirements for primordium was 69 days, and the mushroom's individual weight was 143 g.
Physiological Characteristics of Hericium erinaceus in Sawdust Media
Chang, Hyun-You ; Roh, Mun-Gi ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 252~255
This study was carried out to investigate the physiological charateristics of Hericium erinaceus in sawdust media. The optimum temperature was
and the optimum pH was 5. Mycelial growth and density of H. erinaceus was quite good when oak tree sawdust were used as cultural substrates. The best mycelial growth in H. erinaceus were observed when wheat pollard was added as supplement on sawdust substrates. The optimum supplement ratios of wheat pollard and magnecium sulfate were 20% and 0.1% respectively, for H. erinaceus.
Analysis of Genetic Relationship of Cordyceps militaris in Korea by Random Amplified Polymorphic DNA
Sung, Jae-Mo ; Kim, Sang-Hee ; Yoon, Chul-Sik ; Sung, Gi-Ho ; Kim, Yong-Wook ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 256~273
Seventy two isolates of Cordyceps militaris collected from 11 sites in Korea, including two isolates from ATCC, were used to assess genetic variation within Cordyceps militaris. The anamorph stage and cultural characteristics of C. militaris were observed through microscope and investigated on PDA respectively. The anamorphs of C. militaris were identified to be Verticillium. Isolates of C. militaris showed different growth rates, morphology and color. Fifty six isolates of single ascospore and seventy two isolates of mass ascospore from C. militaris were analysed using by Random Amplified Polymorphic DNA (RAPD) for genetic relationship analysis. Fifty six single ascospore isolates fell into two groups by phenogram constructed from distance values using the UPGMA method in NTSYS-pc software: group A from artificial fruit body of C18 except for isolate 51; group B from artificial fruit body of C738. The average genetic distance value within group A is 0.150 and group B is 0.163. The average genetic distance value between the two groups is 0.221. The average genetic distance value within 56 single ascospores is 0.207 and 72 mass ascospores is 0.330. Genetic relationships were not found among 72 mass ascospore isolates obtained from eleven geographically distant populations.
Phylogenetic Relationships of Genera Coprinus and Psathyrella on the Basis of ITS Region Sequences
Park, Dong-Suk ; Go, Seung-Joo ; Kim, Yang-Sup ; Seok, Soon-Ja ; Ryu, Jin-Chang ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 274~279
The internal transcribed spacer regions(ITS) of the ribosomal DNA gene repeat from Coprinus and Psathyrella spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. rhizophorus, C. radians, C. echinosporus, C. disseminatus, Psathyrella candolleana, P. spadiceogrisea and Stropharia rugosoannulata were compared. The spacer region I and II were
in length respectively and partially contained 17S, 5.8S and 25S. The reciprocal homologies of ITS sequences among these strains were in the range of
. According to the analysis of ITS sequences, Coprinus and Psathyrella spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. Cluster II comprised C. micaceus, C. radians, C. disseminatus, Psathyrella candolleana, and P. spadiceogrisea. On the other hand C. comatus is in Cluster III with Stropharia rugosoannulata even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that taxonomic position of Psathyrella would better be inculded in genus Coprinus. Coprinus comatus, the type species of Coprinus, gives a doubt on monophyletic evolution and is assumed to be paraphyletic or polyphyletic.
Gray Mold Rot on Fruit of Cucumis melo var. reticulatus Caused by Botrytis cinerea
Kwon, Jin-Hyeuk ; Kang, Soo-Woong ; Son, Kyeng-Ae ; Bae, Dong-Won ; Park, Chang-Seuk ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 280~282
A new disease on mask melon grown under plastic film houses was found in Namhae area in May of 1999. Gray to dark brown mold were grown on the surface of matured fruits and infected inside tissues were discolored and rotten. Basal part of the fruit and blossom-end were frequently infected and colonized by fungi. About 2.2% of matured fruits were infected in the surveyed plastic film houses. The causal organism was isolated from the lesion and identified as Botrytis cinerea. The conidia in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized
. Hyaline or pigmented conidiophores were tall, slender and determinated and, sometimes branched irregularly in upper part. Enlarged or rounded apical cells bear conidial cluster and sized
. Optimum temperature for mycelial growth was recorded at
. This is the first report on gray mold of melon caused by Botrytis cineria in Korea.
Impact of Environmental Factors on in vitro Interactions and Niche Overlap between Aspergillus ochraceus and other Storage Fungi
Lee, Hyang-Burm ; Magan, Naresh ; Yu, Seung-Hun ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 283~288
The effect of water activity (
) and temperature (
C) on in vitro growth and interactions between ochratoxin-producing Aspergillus ochraceus and six other fungi (Alternaria alternata, Aspergillus candidus, A. flavus, A. niger, Eurotium amstelodami, E. rubrum) isolated from maize grain were investigated. A. ochraceus and each six other species were paired and their interactions given a numerical score to obtain an index of dominance (
) for each species. Generally A. ochraceus was very competitive and dominant against other fungi. It was, however, dominanted by Alternaria alternata and A. niger at high
, and mutually antagonistic when paired with E. amstelodami and E. rubrum at low
. The growth rates of each species were also calculated under the same range of environmental conditions. They were markedly influenced by aw and temperature. At high temperature (
), A. ochraceus grew most rapidly under slightly drier conditions (
), while A. alternata, A. flavus and A. niger did at high water availability level (
, and high
), A. alternata grew fastest, while A. candidus, E. amstelodami and E. rubrum grew very slowly. Using Biolog plates the effect of
and temperature on utilization patterns of carbon sources in maize was evaluated. The niche overlap index (NOI) relative to A. ochraceus was determined and compared with that of each interacting species. Under high water available condition (
). the NOI of A. ochraceus was often >0.9, indicative of the coexistence with other interacting species. However, against E. amstelodami and E. rubrum at
, the species had NOI <0.8, indicative of occupation of different niches. At low
, NOI for A. ochraceus was <0.8 when paired with A. alternata and A. niger also suggested the occupation of different niches.
Isolation Method of Mushroom Infesting Pests from Mushroom-Growing Compost
Lee, Heung-Su ; Kim, Kyu-Jin ; Song, Geun-Woo ; Kim, Jin-Ho ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 289~292
We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipes, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of seperating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was
mesh; Lycoriella mali was trappped mainly
mesh sieve, Coboldia fuscipes was caught mainly
mesh sieve, Histiostoma sp. was trapped mainly
mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.
Chemical Composition and Biological Activities of Immunostimulants Purified from Alkali Extract of Poria cocos Sclerotium
Rhee, Sang-Dal ; Cho, Soo-Muk ; Park, Jeong-Sik ; Han, Sang-Bae ; Jeon, Young-Jin ; Kim, Hwan-Mook ; Kim, Gwang-Po ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 293~298
An one percent sodium carbonate extract prepared from sclerotia of Poria cocos activated the proliferation of the T lymphocytes as measured by mixed lymphocyte responses(MLR). The active fraction, PCSC22, was isolated from an one percent sodium carbonate extract by a combination of fractionation procedures, including ethanol precipitation and chromatographies on column of DEAE-cellulose and Sephadex G50. Carbohydrate and peptide contained in PCSC22 were 78 : 22% in ratio. On employing gel filtration high performance liquid chromatography, PCSC22 exhitited a homogeneous peak with an average molecular weight of 8 kDa. The sugar moiety of PCSC22 was composed with mannose (92%), galactose (6.2%) and arabinose (1.3%), which might be indicated as heteromannan. Fifteen amino acids were found in peptide moiety of the polysaccharide and aspartic acid, serine, and valine were major components. PCSC22 activated the primary proliferation of T lymphocytes measured by mixed lymphocyte responses, the antibody production of the B lymphocytes and the secretion of nitric oxide from macrophage cell line, RAW264.7.
Purification of Phytase from Aspergillus ficuum and Production of Anti-phytase Antibody
Kim, Keun ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 299~303
Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 22.214.171.124) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.
Batch Kinetics of Exo-polysaccharide Production by Submerged Cultivation of Ganoderma lucidum
Lee, Shin-Young ; Lee, Hak-Su ; Park, Heung-Cho ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 304~311
Batch kinetics during the exo-polysaccharide (EPS) fermentation of Ganoderma lucidum was investigated as a function of different substrates (glucose and starch), substrate concentration
and subculture (3 times). Logistic model for mycelial growth fitted the experimental data better than Monod and two thirds power model. The Luedeking-Pirt equation was adequate to fit the kinetic data of product formation and substrate consumption. The EPS production was strongly non-growth associated, although it was mixed type. The product formation and sustrate consumption by growth associated mechanism decreased as the concentration of glucose increased, while those of the non-growth associated mechanism increased. However, starch medium increased the growth associated and non-growth associated substrate consumption indicating higher availability of substrate. Also, batch culture in starch medium showed the higher specific growth rate and stability during subculture than those in glucose medium. In conclusion, the enhanced EPS production and stability in the subculture was found to be remarkably improved by use of starch as sole carbon source in medium. The maximum mycelium dry weight and EPS production of 9.463 and 10.410 g/l, respectively, were obtained after shake culture of 7 days at
from the media containing 7% starch.
Inhibitive Effects of Meju Extracts Made with a Single Inoculum of the Fungi Isolated from the Traditional Meju on the Human Leukemia Cell Line
Han, Jung ; Kim, Hyun-Jeong ; Lee, Sang-Sun ; Lee, In-Seon ;
The Korean Journal of Mycology, volume 27, issue 4, 1999, Pages 312~317
In order to study the antitumoral effect of meju extracts, which was made with a single inoculum of the microorganism, the cytotoxicity effects on several human leukemia cells such as promyelocytic leukemia cell (HL60), histiocytic lymphoma cell (U937) and acute T-cell leukemia Jurkat cell, and lymphocyte were analyzed by MTT assay. Twenty one microbes, mainly fungal genera, were isolated from Korean traditional mejus of different regions. From those collected isolates, meju was manufactured and extracted with 80% methanol, respectively. Meju methanol extracts exhibited low activites in cytotoxicity tests on HL60 cell, but high antitumoral effects of meju methanol extracts were shown on U937 and Jurkat cells. Meju methanol extracts made with a genera of Mucor, Absidia and Aspergillus showed prominant cytotoxic activities, especially. However all these extracts had no inhibitory effects on the cell growth of lymphocyte under the same conditions.