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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 28, Issue 2 - Oct 2000
Volume 28, Issue 1 - Apr 2000
Selecting the target year
Effect of Rice Bran Added at Spawn-making on the Cultivation of Oyster Mushroom, Pleurotus spp.
Jhune, Chang-Sung ; Kim, Gwang-Po ; Shin, Cheol-Woo ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 1~5
This experiment were carried out to investigate the effect of the rice bran added into spawn various amounts on the cultivation. Our results show that 10 to 20 percent addition of the rice bran as a supplement results in a good mycelial growth and density. However we didn't find a significant variance among the different species of oyster mushroom using poplar sawdust as a medium. When it inoculated spawn with various amounts of rice bran on the medium of rice straw, the mycelial density was increased according to the increase of the added supplement, while there was no significant in the mycelial growth among the treatments. Through the field test it was showed that 15 to 20 percent addition of the supplement results in the highest yield, the shortest days from spawing to pinhead, and the lowest infection rate.
Cultural Characteristics and Fruitbody Formation of Phellinus gilvus
Rew, Young-Hyun ; Jo, Woo-Sik ; Jeong, Ki-Chae ; Yoon, Jae-Tak ; Choi, Boo-Sool ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 6~10
For artificial cultivation of Phellinus gilvus (Schw.) Pat we have conducted a study on cultural characteristics and condition of fruitbody formation. The optimum temperature was about
for mycelial growth. Optimum sawdust media were oak sawdust+willow sawdust(5:5, V/V), oak sawdust+willow sawdust+rice bran (4.5:4.5:1, V/V) and oak sawdust+pine sawdust+rice bran(4.5:4.5:1, V/V) and, the spawn incubation period was about
days. Mycelial growth in the inner portion of oak log was 200 mm after 60 days and duration for the first fruitbody primordia were formed about 90 days after inoculation.
Cultural Characteristics and Fruitbody Formation of Phellinus pini
Rew, Young-Hyun ; Jo, Woo-Sik ; Jeong, Ki-Chae ; Yoon, Jae-Tak ; Choi, Boo-Sool ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 11~15
For artificial cultivation of Phellinus pini (Thore. Fr.) Ames, we conducted some study on mycelium growth and optimum condition for fruitbody formation. The optimum condition for mycelial growth was
. Optimum sawdust media were oak sawdust+willow sawdust+rice bran (4.5:4.5:1, V/V) and oak sawdust+pine sawdust+rice bran (4.5:4.5:1, V/V) and the optimum spawn incubation period was about
days. Mycelial growth in the inner portion of oak log was 40 mm after 60 days and duration for first fruitbody primordia formation was about 110 days after inoculation.
Application of Super Water Absorbent for Edible Mushroom Production
Kim, Myung-Kon ; Yoon, Sook ; Mun, Sung-Pil ; Kim, Hyung-Moo ; Chang, Tae-Bok ; Hong, Jae-Sik ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 16~21
Super water absorbent (CPAM-AS-hyd-1) was prepared by polymerization of acrylamide and allyl sulfonate salt with N,N'-methylene-bis-acrylamide as crosslinking agent, followed by alkaline hydrolysis and the effect on mycelial growth and sporophore production of edible mushrooms in the artificial cultivation was examined. The mycelial growth of edible mushrooms did not depend on the addition of super water absorbent upto 200 g of hydrated polymer gel per 100 cc medium. The proper hydrated polymer gel concentration for sporophore production of Pleurotus sajor-caju and Hericium erinaceus were 200 g and
per 100 cc medium, respectively. The proper hydrated polymer gel and puffed rice hull concentration for sporophore production of Flammulina velutipes was 200 g per 100 mm medium and 10% (v/v), respectively.
Nucleotide Sequence of Mating Locus of Schizophyllum commune Indigenous to North America
Park, Dong-Cheol ; Lee, Sang-Sun ; Lee, In-Seon ; Kim, Hyeun-Jeong ; Lee, Kap-Rang ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 22~25
This study was carried out to compare DNA sequence of mating type locus concerning with direct formation of fruiting body in Schizophyllum commune which is growing in North America with that of same species growing in South America. The nucleotide sequence appeared to have about 96% homology to 1-71
allele from South America strain, showing a conservative feature. The polypeptide sequence showed about 82% homology when compared partially with mating activity region of 1-71
allele. In addition, this polypeptide sequence indicated 74% and 82% identity in homeodomain and acidic-rich regions known as a transcription factor respectively.
Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes
Kim, Dool-Yi ; Azuma, Tomo-Nori ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 26~31
We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.
A Study on the Genetic Variations of Tricholoma matsutake Collected from Eleven Sites of Korea Using I-SSR PCR
Cho, Duck-Hyun ; Lee, Kyung-Joon ; Han, Sim-Hee ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 32~37
The objectives of this study was to identify genetic variations of Tricholoma matsutake (S. Ito et Imai) Sing. growing in different geographic ranges in South Korea. Mushrooms were collected during fruiting seasons from 1994 to 1997 from 11 major sites which included four sites (Bonghwa, UIjin, Goryoung, and Chungdo) in Kyongbuk Province, three sites (Changnyung, Hadong, and Hamyang) in Kyongnam Province, two sites (Yangyang and Inje) in Kangwon Province, one site (Goisan) in Choongbuk Province, and one site (Namwon) in Chonbuk Province. Two mushrooms each from three to eight shiros in each sites were collected. Genetic characteristics were analyzed by Inter-Simple Sequence Repeat Polymerase Chain Reaction (I-SSR PCR) method using six primers. With a total of 131 DNA bands identified, Nei's genetic distance and UPGMA tree were constructed. It was estimated that genetic variations between sites amounted to 12.9%, while 87.1% of total variation was explained by variations among individuals within sites. The cluster analysis indicated that the eleven major sites were clustered into four groups, group I (Yangyang, Hamyang, Inje, Hadong and UIjin), group II (Changnyung, Namwon and Chungdo), group III (Goryoung), and group IV (Bonghwa and Goisan). It is concluded that matsutake mushrooms in South Korea have a considerable degree of genetic variations between major sites.
Observation of Anamorph (Libertella sp.) and Teleomorph (Diatrype stigma) of D. stigma Affecting Bed-log of Oak-mushroom in Korea
Bak, Won-Chull ; Lee, Bong-Hun ; Yoon, Kab-Hee ; Ka, Kang-Hyeon ; Choi, Joong-Sigk ; Lee, Tai-Soo ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 38~40
Anamorph and teleomorph of Diatrype stigma on the bed-log inoculated with oak-mushroom (Lentinula edodes) in Korea were observed and identified. As the characters of anamorph of Diatrype stigma, conidia were hyaline, falcate or slightly curve, and
, and conidia layer was confirmed under the bark. About teleomorph, perithecia
, with ostiole (
, three- to four-sulcate). Size of ascus containing 8 ascospores was
. Ascospores were hyaline to pale brown,
and mostly biguttulate.
Histological Observation on the Symbiotic Relationships Between Gastrodia elata and Rhizomorph of Armillaria mellea
Kim, Yong-Kyoo ; Km, Myung-Kon ; Yoon, Sook ; Hong, Jai-Sik ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 41~45
The histological studies were conducted to investigate the symbiotic relationships between Gastrodia elata and Armillaria mellea by using light and electron microscopes. The fungus, A. mellea, penetrated into the cortex of G. elata, in which endomycorrhizal mycelia in the cortical cells appeared to be dissolved and digested, and seemed to be consequently used as nutritional sources for G. elata growth. Staining of infected tissues revealed that protein- and fat-like substances were localized in the cells. The nuclei of cells infected by the fungal mycelia were hypertrophied 1.5 to 2 times as those without the fungal infection.
Gray Mold of Safflower Caused by Botrytis cinerea
Kwon, Jin-Hyeuk ; Kang, Soo-Woong ; Son, Kyung-Ae ; Park, Chang-Seuk ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 46~48
The gray mold disease was severely occurred on safflower grown in experimental farm of Kyongsangnam-do Agricultural Research and Extension Services in 1999. At first, the infected leaves discolored gray or dark from the tip and gradually expanded to downword. The fungus also attacked stem, pod and calyx. The infected area became dark and dried, and frequently gray mold grown on the infected area. The causal organism was isolated and proved its pathogenecity according to Koch's postulate. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized
. Conidiophores were observed with their wide size of
. The temperature range for mycelial growth was between
with the optimum temperatures of
. The causal organism was identified as Botrytis cinerea and based on mycological characteristics examined. This is the first report on gray mold of safflower caused by Botrytis cinerea in Korea.
Survey and Control of the Occurrence of Mycotoxins from Post-harvest Fruits. II. Detection and Control of the Occurrence of Penicillium Mycotoxins Producing Pathogen in Storaged Fruits (Apple, Pear, Citrus and Grape)
Paik, Su-Bong ; Chung, Ill-Min ; Yu, Seung-Hun ; Kim, Eun-Yong ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 49~54
This study was conducted to investigate the major mycotoxins occurred during storage from apple, peat, citrus and grape. Analyses of the mycotoxins were conducted by TLC and HPLC. Patulin was only detected from apple, pear, citrus and grape infected by penicillium mycotoxins, but citrinin did not detected. The detected amount except grape ranged from
in citrus, respectively. When compared to the effect of control on penicillium mycotoxins during storage in apple, pear and citrus, sodium hypochloride gas and heat at
treatment exhibited effective control. Especially, heat at
treatment exhibited 100% effective control without any injuring by treatment. In pear and citrus sodium hypochloride gas treatment caused injury by treatment.
Antioxidation and Anticancer Effects of Polyozellus multiplex
Han, Jung ; Lee, In-Seon ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 55~59
This study was carried out to investigate the antioxidative and chemopreventive effects of the extracts from Polyozellus multiplex, an edible mushroom through in vitro and in vivo assay. Polyozellus multiplex fractions were assayed for its antioxidative effect with colony formation assay. Polyozellus multiplex methanol extract and water fraction showed protective effects against the cytotoxicity of
. The modifying effects of Polyozellus multiplex methanol extract and water fraction on the induction of carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were investigated in Wistar rats. The GSH content was decreased by MNNG treatment but was increased by adding Polyozellus multiplex water fractions. Also the activity of glutathione S-transferase and the superoxide dismutase levels were increased by the treatment of Polyozellus multiplex water fractions more than with MNNG alone. In addition to the Polyozellus multiplex water fraction increased the p53 expression as compared with the value of MNNG alone.
Purification and Characterization of Fibrinolytic Enzymes from Tricholoma saponaceum
Kim, Jun-Ho ;
The Korean Journal of Mycology, volume 28, issue 1, 2000, Pages 60~65
Two fibrinolytic enzymes were purified from the fruiting bodies of Tricholoma saponaceum. The enzymes have a molecular weight of 18(FE-1) and 18.2(FE-2) kDa, respectively, and include
ion as determined by ICP/MS. The N-terminal amino acid sequence of the two enzymes were exactly the same: A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V. The activity of FE-1 was highly inhibited by EDTA and 1,10-phenanthroline, indicating that the enzyme is a metalloprotease. The activity of FE-1 was slightly increased by
, however, the enzyme activity was totally inhibited by
. Addition of
reversed the inhibition caused by 1,10-phenanthroline. It has a pH optimum at pH 7.5, suggested that FE-1 was a neutral protease. It shows the maximum fibrinolytic activity at
, is completely inactivated above at