Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 28, Issue 2 - Oct 2000
Volume 28, Issue 1 - Apr 2000
Selecting the target year
Influence of Soaking Time of Paddy Straw on the Mycelial Growth of Oyster Mushroom, Pleurotus spp.
Jhune, Chang-Sung ; Choo, Chang-Ho ; Chang, Gap-Yeul ; Paik, Su-Bong ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 67~72
The purpose of this study was to investigate the change of water content in mushroom substrate, paddy straw, and the mycelial growth of oyster mushroom by various soaking time. The water content of paddy straw was gradually increased as the soaking time was getting longer. These experiments also showed that the water content in the upper part of paddy straw was less than that of the low part. The mycelial growth was the good between 4 and 12 hours soaking treatment, showing faster growth and higher density in the lower part than the upper part. However we could not found any significant variation among 5 strains of oyster mushroom in the effects of the various soaking times and soaking part. The pH of the lower part substrates had a low pH compared with that of the upper part. When pathogens and mushroom mycelia were co-inoculated on paddy straw under various soaking conditions, the mushroom mycelial growth showed fast and high density according to the increase of the soaking time. The experiment related to water transport reveals that it is not easy to move into upper part, but only soaked part by adding water is available to absorb enough water. Old straw was much better than the straw new this year one for water absorption, mycelial growth and mycelial density.
Studies on Mycelial Growth and Artificial Cultivation of Pleurotus eryngii
Kang, Mi-Sun ; Kang, Tae-Su ; Kang, An-Seok ; Shon, Hyeong-Rak ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 73~80
This study was carried out to investigate the optimal mycelial growth and an artificial cultivation conditions of Pleurotus eryngii. The optimal medium for the mycelial growth and density was MYPA medium. The optimal temperature and pH for the mycelial growth were
and 6.0, respectively. The modified optimal medium composition were obtained to be soluble starch 3%(w/v), malt extract 0.25%(w/v), yeast extract 0.25%(w/v) and
0.05% (w/v). From the results of experiment on the nutritional requirements, the modified optimal medium was higher than MYP medium in mycelial production and growth yield (Yx/s) of Pleurotus eryngii. The optimal sawdust species of solid culture for the mycelial growth and density was Quercus spp. The optimal concentration of additives (rice bran and wheat bran) and moisture content for the mycelial growth were about 30%(v/v) and 70%(v/v), respectively. On the other hand, the optimal concentration of additives for the production of fruiting body was 20%(v/v) of rice bran.
Investigation on Cultural Characteristics of Mycelial Growth by Cordyceps scarabaeicola
Lee, Jae-Keun ; Choi, Young-Sang ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 81~87
The optimal synthetic medium, temperature and pH range for the mycelial growth of Cordyceps scarabaeicola were PDA,
, and pH
, respectively. The effects of carbon sources, nitrogen sources and mineral salts on mycelial growth were superior to lactose, ammonium phosphate and
, respectively. Liquid spawn culture was selected out BM(brown sugar 3%, soybean flour 0.3%, potassium phosphate 0.05%, magnesium sulfate 0.05%) medium.
Studies on Improvement of Cultural Practice for Lyopyllum ulmarium
Chi, Jeong-Hyun ; Park, Woo-Kill ; Kim, Young-Ho ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 88~92
Substrate improvement, maturation period and method of pinheading promotion were investigated to establish the culture method of Lyopyllum ulmarium which could obtained high yield in the short culture period. Rice bran+wheat bran (10%+10%) combination was selected from various additives, which showed vigorous growth of fruit body and high yield (133.8 g/850 cc). Oyster shells powder substance increased yield to 155.5 g per 850 cc capacity bottle. The suitable method for pinheading promotion was to stand the bottle reversely. The number of fruit bodies per 850 cc capacity bottle in this method was 27.2 stipes and bunch formation was good. When the maturation period of mycelium culture was 15 days to 30 days, pinheading period was 10 days. But when the maturing mycelium was 30 to 45 days, yield per 850 cc bottle was 148.2 g.
Effect of Slow Releasing Fertilizer Supplement in Oyster Mushroom (Pleurotus ostreatus) Cultivation
Rew, Young-Hyun ; Jo, Woo-Sik ; Kim, Chan-Yong ; Yoon, Jae-Tak ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 93~96
To investigate the effect of inorganic nutrient supplement in oyster mushroom (Pleurotus ostreatus), we have conducted some study on cultural and growth characteristics of fruitbody formation and chemical composition of media and fruitbody. When supplemented with slow releasing fertilizer, contamination rate was not different from non-supplemented medium, days for incubation time and first pinhead were faster than non supplemented medium. And fruitbody yield and biological efficiency were increased
respectively, but biological efficiency was decreased when increased supplement ratio. The chemical compositions (total carbon, total nitrogen, ammonia nitrogen, nitrate nitrogen, potassium and phosphate) of slow releasing fertilizer supple mented medium and fruitbody were compared with non-supplemented.
The Characteristics of Thermophilic Fungi in Relation to Growth-Promoting Effect on the Mycelium of Pleurotus ostreatus
Lee, Ho-Yong ; Shin, Chang-Yup ; Kim, Jun-Ho ; Kim, Won-Rok ; Lee, Young-Keun ; Chang, Hwa-Hyoung ; Song, In-Geun ; Hyun, Soung-Hee ; Min, Bong-Hee ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 97~102
The mycelial growth of Pleurotus ostreatus in compost is strongly stimulated by solid-state fermentation with thermophilic fungi which were isolated from oyster mushroom compost. The biochemical characteristics of these thermophilic fungi were investigated. Cellulase and ligninase activities were not detected by clear zone effect on CMC and lignin media. All of thermophilic fungi grew well with high mycelial density on xylan media and the growing rate of Sepedonium sp. S-2 observed very high. In results of MUF-test, extracellular enzyme activity of Sepedonium sp. S-2, and S-5 measured very high. On the compost after high temperature fermentation with Sepedonium sp. S-2 and S-5, the mycelial growing rate of Pleurotus ostreatus was increased about 50% and it also showed the inhibiting effect on mycelial growth of Trichoderma sp. SJG-51. Isolated thermophilic fungi, Sepedonium sp. S-2 and S-5 were expected as very useful organism for making oyster mushroom compost.
Effect of Pasteurized Substrates in Various Condition on the Mycelial Growth of Oyster Mushroom, Pleurotus spp.
Jhune, Chang-Sung ; Shin, Dong-Hun ; Park, Jeong-Sik ; Oh, Se-Jong ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 103~108
Various conditions for mushroom were investigated to study their effects on the mycelial growth of oyster mushroom and pathogens. Our results show that the optimal pasteurization temperatures for mycelial growth and pathogens are
respectively. Both were then decreased by heating over
and getting down temperature under
, respectively. In the experiment for pasteurization time, it was showed that both 8 and 12 hours pasteurization were greater than 24 hours treatment for the mycelial growth. In contrast, we didn't find any particular effect by different soaking time of substrates. The pH In substrates was leveled well by pasteurization and after-fermentation even though the acidity was lowered by extending the soaking time. Our results also show that the mycelial growth is greatest at
regardless of pasteurization time for substrates, Through the experiment with paddy straw as a substrate, it was showed that two time pasteurization was greater than one time for mycelium growth and pro-fermentation of paddy straw before pasteurization have resulted in worth mycelial growth compared with that of normal condition.
Changes of Fatty Acid Composition by Various Developmental Stage and Fruit Body Section in Pleurotus ostreatus
Rew, Young-Hyun ; Lee, Sook-Hee ; Jo, Woo-Sik ; Yoon, Jae-Tak ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 109~111
Composition of fatty acids (FAs) in Pleurotus ostreatus were analyzed by gas chromatography and compositional changes according to cultivar, fruitbody section and developmental stage (days after primordia formation) were investigated. Major FAs in oyster mushroom were linoleic acid, oleic acid and palmitic acid. Unsaturated FAs were higher in pileus portion than upper- or lower-stipe portion. Oleic acid content was increased with developmental days but linoleic acid content was highest at
days after primordia formation and then decreased after that. And ratio of unsaturated FAs/saturated FAs was decreased with basidiocarp maturation.
Genetic Relationships of Internal Transcribed Spacer (ITS) Regions on Entomopathogenic Fungi by RFLP
Choi, In-Young ; You, Young-Jin ; Choi, Joung-Sik ; Lee, Wang-Hyu ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 112~117
A similarity coefficient were analyzed by RFLP of fourteen species of entomopathogenic fungi, isolated from inhabiting pupa and adult insect at forest. Each rDNA ITS I and ITS II with primers of ITS 1 and ITS 4 was amplified by PCR. The amplified products were conserved to 500 bp were not demarcated between genus and species. Four Paeciliomyces tenuipes, two Beauveria bassiana and six Cordyceps militaris were treated by seven restriction enzymes and confirmed in species except JB3 by electrophoresis band. However, the band of C. scarabaeicola showed the identity with B. bassiana. The result of this experiment indicated that the teleomorph of C. scarabaeicola was the same as that of B. bassiana. CfoI and HpaII of seven restricted enzymes were easily discriminating in the genus between Paecilomyees and Cordyceps. Especially, CfoI was more effective to classify the genera of Paecilomyees, Cordyceps and Beauveria than other restriction enzymes. The band patterns of RFLP of P. tenuipes, C. militaris, C. scarabaeicola and B. bassiana were also analyzed by UPGMA program of NTSYS-pc and showed 100% significance. Thus, the similarity coefficient tended to be lower between genera by RFLP analysis, but was higher between species.
A Newly Identified Hypogeous Mushroom of Korea, Melanogaster tuberiformis
Castellano, Michael ; Kim, Je-Su ; Koo, Chang-Duck ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 118~120
Sclerotium rolfsii Causing Collar Rot on Doonggulle (Polygonatum odoratum var. pluriflorum)
Kwon, Jin-Hyeuk ; Kang, Soo-Woong ; Park, Chang-Seuk ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 121~122
A destructive collar rot of Doonggulle (Polygonatum odoratum var. pluriflorum) occurred in an experimental farm at Hamyang Medicinal Plant Experiment Station, Kyonguam ARES in 1999. The disease also observed in farmer's fileds in Hamyang and the infection rate reached up to 12.3%. Upper parts of the infected plants were mostly blighted and white mycelial mats were formed on the lesions or soil lines near the stem. The fungus readily formed sclerotia on PDA and then shapes were globoid or irregular, and sized
). Although the fungus showed wide range of mycelial growth temperature, the optimum temperature was about
. The typical sclerotium type and clamp connections of hyphae were found in the fungus grown in PDA. The causal fungus was identified as Sclerotium rolfsii on the basis of mycological characteristics and pathogenicity that on the host plants. This is the first report on the collar rot of Polygonatum odoratum caused by Sclerotium rolfsii in Korea.
Studies on the Hemolytic Activities of Korean Wild Mushrooms (II) - Screening of 22 Mushrooms Including Laccaria vinaceoavellanea for Their Hemolytic Activities -
Chung, Kyeong-Soo ; Lee, Ji-Seon ; Jo, Moon-Joo ; Lee, Im-Seon ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 123~125
To investigate the hemolytic toxicities of the Korean basidiomycetes, the cold-water extracts of 22 wild mushrooms were tested for their hemolytic activities on the erythrocytes of a mouse and a rat. As a result, three species including Collybia confluence, Laccaria vinaceoavellanea and Lenzites betulina showed strong hemolytic activities. Of these, L. vinaceoavellanea retained more than 80% of its hemolytic activity even after it was boiled for 30 minutes, while the other two were inactivited in five minutes.
Hypoglycemic Effect of Extracts of Soybean Paste Containing Mycelia of Mushrooms in Streptozotocin- Induced Diabetic Rats
Yang, Byung-Keun ; Jeong, Sang-Chul ; Hur, Nam-Jung ; Ha, Sang-O ; Kim, Ki-Young ; Kym, Kyo-Hyeok ; Yun, Jong-Won ; Song, Chi-Hyun ;
The Korean Journal of Mycology, volume 28, issue 2, 2000, Pages 126~129
The hypoglycemic effects of the hot water extract of soybean paste containing mycelia of Phellinus linteus, Cordyceps militaris, Ganoderma lucidum were evaluated in streptozotocin-induced diabetic rats. All the diabetic groups were not significantly affected in food intake and food efficiency ratio. Significant decrease in blood glucose was observed in all the soybean paste containing mushroom mycelia (SPM) groups. Especially, administration of soybean paste containing mycelia of Ganoderma lucidum, blood glucose level was significantly lowered up to 45% compared to that of diabetic control group. The GPT and GOT activities indicated lower values in all the SPM groups than in the diabetic control group.