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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 31, Issue 3 - Dec 2003
Volume 31, Issue 2 - Aug 2003
Volume 31, Issue 1 - Apr 2003
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Changes of Teleomorph of Diatrype stigma Damaging Bed-log of Shiitake and Secondary Harmful Fungi
Bak, Won-Chull ; Lee, Bong-Hun ; Kim, Se-Kwon ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 53~58
DOI : 10.4489/KJM.2003.31.2.053
Studies were conducted to investigate the changes of teleomorph of Diatrype stigma on bed-log of shiitake (Lentinula edodes) and appearance of secondary harmful fungi from December 1999 to August 2001. The color of stroma was changed from red brown to dark brown after 12 months. The size of ostioles became smaller and the number of ostioles decreased as time passed. Most of the ostioles disappeared after 20 months from toleomorph formation. Perithecia and ascospores were gradually disappeared, and perithecia containing ascospores were observed at 10% of bed-logs tested after 20 months. Concerning the secondary harmful fungi infecting the bedlog of shiitake after teleomorph of D. stigma, Hypoxylon truncatum (Anamorph : Nodulisporium sp.), Schizophyllum commune and Trichoderma spp. were observed on stroma of D. stigma. Penicillium sp. occurred on spore-horn of Libertella sp. (Anamorph of D. stigma). Besides, Hypoxylon howeianum and some species of Myxomycetes were observed. Also, Libertella sp. appeared again at some bed-logs.
Characterization of Laccase Purified from Korean Pycnoporus cinnabarinus SCH-3
Park, Eun-Hye ; Yoon, Kyung-Ha ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 59~66
DOI : 10.4489/KJM.2003.31.2.059
Laccase produced by Pycnoporus cinnabarinus SCH-3 isolated from Korea was partially purified using ultrafiltration, anion exchange chromatography and affinity chromatography, The laccase was produced as the predominant extracellular phenoloxidase during primary metabolism. Neither lignin peroxidase nor manganese-dependent peroxidase were detected in the culture fluid. In order to examine the effect of inducers in laccase production, 2,5-xylidine was added in the culture of Pycnoporus cinnabarinus SCH-3. Addition of 2,5-xylidine enhanced 25-fold laccase production. Purified laccase was a single polypeptide having a molecular mass of approximately 66 kDa, as determined by SDS-polyacrylamide gel electrophoresis, and carbohydrate content of 9%.
values for laccase with ABTS [2,2-azinobis (3-ethylbenzthiazoline 6-sulfonic acid)] as a substrate (Lineweaver-Burk plot) was determined to be
, respectively. The optimal pH for laccase activity was found to be 3.0. The enzyme was very stable for 1 hour at
. Half-life (
) of the enzyme was about 10 min at
. Spectroscopic analysis of purified enzyme indicated that the enzyme was typical of copper-containing protein. Substrate specificity and inhibitor studies for laccase also indicated to be a typical fungal laccase. The N-terminal amino acid sequence of the P. cinnabarinus SCH-3 laccase showed 94% of homology to the N-terminal sequences of laccases from P. cinnabarinus PB and P. coccineus.
Characteristics of Antimicrobial Activities for the Human Pathogenic Microorganism by Extracts from Korean Mushrooms
Kim, Sung-Tae ; Lee, Kang-Hyeob ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 67~76
DOI : 10.4489/KJM.2003.31.2.067
This study was performed to screen antimicrobial activities of 198 extracts from 66 Korean mushrooms against 19 human pathogenic microorganisms using paper disc method. Mushrooms were extracted with petroleum ether 80% ethanol and distilled water in that order Among the extracts with antimicrobial activities, 1 water extract of Amanita virgineoides, 8 ethanolic extracts including Amanita and 1 petroleum ether extrac of Psathyrella hydrophila were highly active against fungi, respectively. In addition to, 24 extracts including Amanita pseudoporphyria, Amanita spissacea, 3 extaracts including Paxillus curtisii were highly active against Gram negative and positive bacteria, respectively.
Studies on the Cultural Characteristics of Cellulase Production by Roseofomes subflexibilis
Chang, Hyung-Soo ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 77~83
DOI : 10.4489/KJM.2003.31.2.077
For the purpose of utilizing cellulose resources by cellulolytic enzymes of Roseofomes subflexibilis, it's cultural conditions for the production of cellulolytic enzymes in synthetic media were investigated. The results obtained were summarized as follows : The optimum temperature and pH for the enzyme production were
and pH 4.0, respectively. Among the carbon sources, glucose was good for the production of cellulase. The optimum concentration of saccharose as 1.2%. As a organic nitrogen source, yeast extract was good for the mycelial growth. The optimum concentration of yeast extract as 1.5%. As a inorganic nitrogen source,
was good for the mycelial growth. The optimum concentration of
were 1.1%. The mineral salt of
was effective and the optimum concentration was 0.1 M.
Cultural Characteristics of Pycnoporus coccineus and P. cinnabarinus
Ka, Kang-Hyeon ; Lee, Jeong-Hee ; Hur, Tae-Chul ; Yoon, Kab-Hee ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 84~88
DOI : 10.4489/KJM.2003.31.2.084
Basic studies on the cultural characteristics of Pycnoporus coccineus and P. cinnabarinus were performed. They exhibited
optimal temperature ranges and optimal pH ranges of
. Among 6 media, they were good at mycelial growths on PDA, LBA and YMA.P. coccineus grew more than P.cinnabarinus on the same medium. Among 10 sawdust media, they were good at mycelial growth on three oak trees and Alnus hirsuta. However, the sawdust of Castanea crenata was bad at mycelial growth. Among 3 coniferous trees, Larix leptolepis showed better growth than the other trees such as Pinus densiflora and P. koraiensis. The fruit body production P. coccineus was about twice better than P. cinnabarinus on Quercus spp. sawdust cultivation.
Comparison of Growth Characteristics of Tricholoma matsutake Mycelium Among the Types of Air Bubble Bioreactor
Lee, Wi-Young ; Ahn, Jin-Kwon ; Ka, Kang-Hyeon ; Kwon, Young-Jin ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 89~93
DOI : 10.4489/KJM.2003.31.2.089
In order to select suitable bioreactor type inhibiting cell stress during submerged culture of Tricholoma matsutake mycelium, the growth characteristics and ergosterol contents were investigated using the external-loop type of air-lift bioreactor (ETAB), balloon type of air bubble bioreactor (BTBB) and column type of air bubble bioreactor (CTBB). Dry weights of the T. matsutake in the BTBB, ETAB and CTBB were 12 g, 11.4 g, and 9.5 g per 1 litter, respectively. BTBB, ETAB and CTBB reached stagnant phases 16, 20, and 24 days after cultivation, respectively, The BTBB was more suitable for liquid culture of T. matsutake mycelium compared to other bioreactors owing to much mycelia product and short culture period. The ergosterol contents produced by the mycelium in the bioreactors were in sequence of BTBB, CTBB, and ETAB at every growth phase. BTBB might affect the mycelium on producing the smallest size of pellets. BTBB and CTBB got the mycelium precipitated and coagulated under operation of bioreactor sparser, whereas ETAB shown no effect of above phenomenon. A renovated bioreactor combined between a balloon shape of BTBB and an external-loop of ETAB was developed to enhance the efficiency of culture technique.
Culture Condition and Inoculum Volume of Liquid Spawn on the Bottled Cultivation of Agrocybe cylindracea
Cheong, Jong-Chun ; Hong, In-Pyo ; Jang, Kab-Yeul ; Park, Jeong-Sik ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 94~97
DOI : 10.4489/KJM.2003.31.2.094
In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium,
temperature and pH 7.0. And, optimum inoculum volume was
. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.
Metalaxyl Sensitivity Related with Distribution Feature of Mating Type of Phytophthora capsici Population from Red Pepper in Korea
Song, Jeong-Young ; Yoo, Sung-Joon ; Lee, Youn-Su ; Kim, Byung-Sup ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 98~102
DOI : 10.4489/KJM.2003.31.2.098
Metalaxyl sensitivity related with distribution feature of mating type was characterized far Phytophthora capsici population, totally 433 isolates of the red-pepper pathogen collected from 75 pepper fields in Korea from 1995 to 1998. At the concentration of metalaxyl
, inhibition rate of mycelial growth of P. capsici isolates was 68.2% in average compared to control, and 28.6% isolates in average were estimated as resistance to the chemical. Isolates of field unit with a single mating type revealed similar level of sensitivity to metalaxyl and showed sensitive or resistant in most field units. However, isolates of field units with both mating types revealed diverse sensitivity level to the chemical and various occurrence ratio of metalaxyl sensitive : resistant in each field unit. Results indicated that different levels of metalaxyl sensitivity of P. capsici population in Korea seem to be closely related with occurrence ratio of A1 : A2 mating type of each field.
Morphological and Molecular Characterization of Alternaria Isolates from Solanaceous Crops
Yu, Seung-Hun ; Cho, Hye-Sun ; Kim, Byung-Ryun ; Park, Myung-Soo ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 103~113
DOI : 10.4489/KJM.2003.31.2.103
Twenty five isolates of Alternaria were obtained from various solanaceous crops in Korea. For all isolates, morphological characteristics of the conidia were determined and compared with those of representative isolates of A. solani and A. tomatophila. A selection of the isolates and the representative Alternaria isolates were evaluated for Pathogenicity to potato, tomato, egg plant and red pepper. Molecular characteristics of 17 isolates of Alternaria inculding the representative isolates were determined using sequence analysis of IRS rDNA and histone H3 gene, and URP-PCR analysis. Based on morphological characteristics, the isolates from the solanaceous crops were grouped as identical or very similar to either A. tomatophila (ATO), A. solani (ASO), and unidentified Alternaria sp. (ASP). Isolates of ASO were moderately pathogenic to all the solanaceous crops tested, but ATO isolates were highly pathogenic to tomato and the ASP isolate was pathogenic only to potato. Among the molecular markers used in this study, the URP-PCR analysis was found to be appropriate for taxonomic resolution of these species. Based on the conidial morphology, pathogenicity test and molecular characteristics, A. tomatophila (early blight of tomato) could be distinguished from A. solani (early blight of potato), and the Alternaria sp. (ASP) from potato, which was closely related to ASO in conidial morphology, was considered as a new species.
Detection of Platelet Aggregation Inhibitors and Fibrinolytic Substances from Mushrooms
Park, Jeong-Sik ; Hyun, Kwang-Wook ; Seo, Seung-Bo ; Cho, Soo-Muk ; Yoo, Chang-Hyun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 114~116
DOI : 10.4489/KJM.2003.31.2.114
Platelet aggregation inhibitory and fibrinolytic activities of water and ethanol extracts from mushrooms were studied. The highest platelet aggregation inhibitory activity was 81.2% in the ethanol extract from fruiting body of Inonotus obliquus ASI 74006 and also were high in the ethanol extract from fruiting bodies of Fomitella fraxinea. The ethanol extract from the mycelia of Agaricus blazei Murill. ASI 1174 showed the strongest fibrinolytic activity as 9.6 unit. However fibrinolytic activities of other mushrooms were low or negligible.
Stem Rot of Eggplant (Solanum melongena) Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Shin, Shun-Shan ; Park, Chang-Seuk ;
The Korean Journal of Mycology, volume 31, issue 2, 2003, Pages 117~119
DOI : 10.4489/KJM.2003.31.2.117
A stem rot of eggplant (Solanum melongena) was found in experimental field of Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The typical symptoms of the disease were stem rot, crown rot, wilt or blight. Upper parts of the infected stems were mostly blighted and white turf of fungal mycelium mats was spread over lesions. Sclerotia were farmed on the stems near soil line. The sclerotia of the fungus eadily were produced in artificial media such as PDA at
. The sclerotia were globoid,
in diameter and brown in color. The optimum temperature for growth of the fungus was about
. The typical clamp connections were found in the hypha formed on PDA, and was
in size. On the basis of mycological characteristics and pathogenecity test, the fungus was identified as Sclerotium rolfsii. This is the first report of stem rot of eggplant caused by Sclerotium rolfsii in Korea.