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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 31, Issue 3 - Dec 2003
Volume 31, Issue 2 - Aug 2003
Volume 31, Issue 1 - Apr 2003
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Taxonomical Classification and Species-specific Detection of Genus Some Phellinus using Phylotype
Kim, Cheng-Yun ; Lee, Jae-Yun ; Kim, Gi-Young ; Lee, Ki-Won ; Park, Jae-Min ; Kim, Mun-Ok ; Lee, Tae-Ho ; Lee, Jae-Dong ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 121~128
DOI : 10.4489/KJM.2003.31.3.121
This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.
Notes on Species of the Laboulbeniales Parasitic on Species of the Dermaptera in Korea
Lee, Yong-Bo ; Na, Young-Hee ; Bong, Pil-Dong ; Lim, Chae-Kyu ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 129~133
DOI : 10.4489/KJM.2003.31.3.129
Two species belonging to two genera of the Laboulbeniales were collected from species of the Dermaptera of Korea. Among these species, Dimeromyces anisolabis Thaxter parasitic on Anisolabis maritima (Bonelli) is new to Korea and Filariomyces forficulae Shanor parasitic on Labidura riparia japonica (do Haan) was collected newly Iron Jeju island. These specimens were preserved in Mycologlcal Laboratory, Division of Science Education, College of Education, Chosun University.
Mushrooms Utilized as Natural Breeding Sites by Wild Drosophilids on Jeju Island, Korea
Kim, Won-Taek ; Oh, Duck-Chul ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 134~140
DOI : 10.4489/KJM.2003.31.3.134
Wild Mushrooms collected in Mt. Halla of Jeiu Island around altitude of
from 1999 to 2000 were studied to investigate utilization as the natural breeding sites by wild drosophilids. Thirty-five mushroom species belonging to the 19 genera of 12 families from 2 orders were used as natural breeding sites by drosophilids. Adult flies emerged from 14 species of mushrooms belonging to the 11 genera of 8 families from 2 orders were identified. From the rest of 21 species of mushrooms, only larvae or/and pupae were reaped. Among 35 species of mushrooms, 34 species belonged to order Agaricales and only one species, Sarcodon scabrosum, belonged to order Aphyllophorales. In terms of composition of mushroom families, total 35 species belonging to 12 families were composed of 10 species of Russulaceae, 6 species of Amanitaceae, 4 species of each Tricholomataceae and Agaricaceae, 3 species of Boletaceae, 2 species of Strophariaceae and each one species of rest 6 families. Concerning the composition of the genus, 35 species belonging to 11 genera were composed of 8 species of Russula, 6 species of Amanita, 4 species of Agaricus, 2 species of Lactarius, and each one species of rest 7 genera. In terms of the mushroomsize preference of drosophilid flies for breeding sites, most flies preferred comparatively large mushrooms above 5 cm of pileus size in diameter, Fourteen species of drosophilids were identified as mushroom-breeding species. Among these, one and five species belonged to genera Mycodrosophila and Hirtodrosophila respectively, The genus Drosophila included the most abundant 8 species. Drosophila bizonata and Hirtodrosophila sexvittata bred on 6 species of mushrooms respectively, D. unispina bred on 3 species of mushrooms, rest species of Drosophila bred on one or two species of mushrooms. From the result of this study and other literatures, it could be presumed that much more mushroom species could serve as the natural breeding sites for greater number of drosophilid species than those examined in this study on Jeju Island.
Studies on the Morphological and Physiological Characteristics of Pleurotus cystidiosus O.K.Miller, the Abalone Mushroom
Jang, Kab-Yeul ; Jhune, Chang-Sung ; Shin, Chul-Woo ; Park, Jung-Sik ; Cheong, Jong-Chun ; Choi, Sun-Gyu ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 141~147
DOI : 10.4489/KJM.2003.31.3.141
The pileus of Pleurotus cystidiosus was attached at the side and was spatular-shape. It grew to the size of
with dark brown color. The length of stem was
. The individual weight of fruiting-body was 25.4 g in average. This study was conducted to Investigate the physiological characteristics and to develop the artificial cultivation technique of P. cystidiosus. To find out the optimum condition of mycelial growth of P. cystidiosus, physiological conditions such as media, temperature, pH carbon sources and nitrogen sources were investigated. Various cultural media were used for the cultivation of P. cystidiosus and compost dextrose agar was favorable for mycelial growth. The optimal growing temperature and pH were in range of 25 to
, respectively. When different carbon sources were compared, glucose was found to be best. However nitrogen sources were different with each strains. When different C/N ratio were compared, 20:1 of C/N ratio was found to be best.
Screening of Mushrooms Having Angiotensin I-Converting Enzyme Inhibitor
Lee, Dae-Hyoung ; Kim, Jae-Ho ; Cheong, Jong-Chun ; Gong, Won-Shik ; Yoo, Young-Bok ; Park, Jeong-Sik ; Yoo, Chang-Hyun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 148~154
DOI : 10.4489/KJM.2003.31.3.148
Extracts from 52 samples of mushrooms were prepared by using water, ethanol and methanol, and then yields and angiotensin I-converting enzyme(ACE) inhibitory activity were investigated. Sample mushrooms contained crude proteins of
, curde lipids of
and carbohydrates of
. Among 52 samples, the water extract from fruiting body of Pholiota spp. ASI 24027 showed the highest extraction yield of 68%. Water extract of Pholiota spp. ASI 24012 fruiting body had potential ACE inhibitory activity of 66%. The optimal extraction condition of the ACE inhibitor from the fruiting bodyies of Pholiota spp. ASI 24012 was In water at
for 1 hr and ACE inhibitory activity was 67.6% on the condition with 0.2 mg of
Studies on Immuno-Modulatory and Antitumor Effects of Crude Polysaccharides Extracted from Paecilomyces sinclairii
Shim, Sung-Mi ; Im, Kyung-Hoan ; Lee, U-Youn ; Kim, Jung-Wan ; Shim, Mi-Ja ; Lee, Min-Woong ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 155~160
DOI : 10.4489/KJM.2003.31.3.155
Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) materials were extracted from Paecilomyces sinclairii. In vitro cytotoxicity tests. Fr. HW was not cytotoxic against MH3T3, Sarcoma 180 and HepG2 cancer cell lines at the concentration of
, while HT-29 cell line was cytotoxic at the concentration of
. Fr. NaCl and Fr. MeOH were slightly cytotoxic to the cell lines. Intraperitoneal injection with Fr. NaCl and Fr. MeOH exhibited antitumor activity with life prolongation effect of 32.3% in mice inoculated with Sarcoma 180. Fr. NaCl improved proliferation of spleen cells and the immunopotentiation activity of B lymphocyte by increasing spleen cell numbers and the alkaline phosphatase activity by
folds, respectively. Fr. NaCl generated
of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of
, while iipopolysaccharide, a positive control, produced
. The Fr. NaCl showed the hightest antitumor effect and activation of B lymphocytes and macrophage. From these results, antitumor effect of P. sinclaitii was likely due to immunopotentiation activity.
The Immuno-Modulatory and Antitumor Effects of Crude Polysaccharides Extracted from Daedaleopsis tricolor
Shim, Sung-Mi ; Im, Kyung-Hoan ; Kim, Jung-Wan ; Lee, U-Youn ; Kim, Ha-Won ; Lee, Min-Woong ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 161~167
DOI : 10.4489/KJM.2003.31.3.161
Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) materials were extracted from Daedaleopsis tricolor. In vitro cytotoxicity tests, Fr. HW was not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2 and HT-29 at the concentration of
, while Fr. NaCl and Fr. MeOH were cytotoxic to the cell lines. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 77.4% in mice inoculated with Sarcoma 180. Fr. NnCl and Fr. HW improved proliferation of spleen cells and the immunopotentiation activity of B lymphocyte by increasing spleen cells and the alkaline phosphatase activity by
folds, respectively. Fr, NaCl generated
of nitric oxide (NO) when cultured with RAW 264.7 at the concentration of
, while lipopolysaccharide, a positive control, produced
. Intraperitoneal injection with Fr. NaCl (50 mg/kg body weight) increased the numbers of peritoneal exudate cells and circulating leukocytes by 10 folds and two folds, respectively, than in the control group. The antitumor effect of D, tricolor was likely due to immunopotentiation activity.
Purification and Characterization of a Chitinase in Culture Media of Cordyceps militaris(Linn.) Link.
Lee, Kang-Hyeob ; Min, Tae-Jin ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 168~174
DOI : 10.4489/KJM.2003.31.3.168
In this study, Cordyceps militaris was grown in a liquid medium containing colloidal chitin. A chitinase was purified from the supernatant or cultured medium by ammonium sulfate fractionation, DEAE-Sephadex A-25 and Sephadex G-50 column chromatography. Optimum temperature and pH of this enzyme were
and 5.5, respectively. The molecular weight of the chitinase was estimated to be 48.5 kDa by SDS-PAGE and its Km value was 0.57 mM. The activity of this enzyme was inhibited by
maleic anhydride, acetic anhydride or N-bromo succinimide, especially strongly inhibited by sodium cyanate for 84.0 percentage. But its activity wag slightly stimulated by
ion, respectively. The products formed during hydrolysis of the hexa-N-acetylchitohexaose with this enzyme were N,N'-diacetylchitobiose and N,N',N'-triacetylchitotriose. These results imply that this purified enzyme may be an endo-chitinase.
Mycelial Growth Using the Natural Product and Angiotensin Converting Enzyme Inhibition Activity of Pleurotus eryngii
Kang, Tae-Su ; Jeong, Heon-Sang ; Lee, Myong-Yul ; Park, Hee-Joeng ; Jho, Taek-Sang ; Ji, Seung-Taek ; Shin, Myung-Keun ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 175~180
DOI : 10.4489/KJM.2003.31.3.175
To develop the health/functional food materials, we investigated the cultural condition of mycelial growth on the solid state fermentation using the brown rise, Acanthopanax sp. and Artemisia sp., and also evaluated inhibitory activity of angiotensin converting enzyme (ACE) of hot water extracts from cultured media of Pleurotus eryngii. As the amount of Acanthopanax nnd Artemisia In the cultural media increased, the mycelial growth rate decreased. Especially, addition of Aeantopanax showed marked effect than Artemisia. Moisture contents in three kinds of cultured media were in the range of
. Crude protein fat and crude fiber content were the highest value in cultured brown rice medium, whereas the mineral contents (Ca, K and P) were higher in the Acanthopanax supplemented (5%) medium than the other media, The extraction yield of the Artemisia supplemented (5%) medium was the highest value of 4.80%, and the pH of hot water extract from cultured brown rice medium showed the lowest value of 6.1. Lightness (L) values in three kinds of extracts from cultured media were in the range of
. Redness (a) value was the highest In the brown rice and Acanthopanax supplemented media, however cultured Artemisia supplemented medium showed the highest value in yellowness (b). In comparison of sugar components analyzed by the thin layer chromatography with three kinds of samples, two spots were detected to be glucose and maltose, respectively. The ACE inhibitory activity of hot water extract from the cultured Acanthopanax supplemented medium showed the highest value at the concentration of
. These results suggest that the Pleurotus eryngii grew in natural media using brown rice and Acanthopanax can be supplemented to the brown rice medium to enhance its ACE inhibitory activity as health/functional food materials.
Production of Laccase by Fomitella fraxinea
Yoon, Jae-Don ; Lee, Jong-Suk ; Lee, Kyung-A ; Chung, Min-Wook ; Ha, Hyo-Cheol ; Lee, Jae-Sung ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 181~186
DOI : 10.4489/KJM.2003.31.3.181
The production of laccase by Fomitella fraxinea was studied. The addition of minerals were necessary far laccase production by Fomitella fraxinea. Jar fermentor and Air-sparging fermentor performed high productivity In laccase activity by F. fraxinea. Laccase activity reached 3,540 in 8 days (Jar fermentor) and 3,100 in 6 days (Air-sparging fermentor) respectively.
Optimal Condition for Mycelial Production of Armillaria mellea
Kim, Myung-Kon ; Choi, Han-Seok ; Park, Hyo-Suk ; Kim, Sung-Jun ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 187~191
DOI : 10.4489/KJM.2003.31.3.187
Armillaria mellea, honey mushroom is well known as a symbiotic fungus with Gastodia elata, The mycelial yields of the fungus were compared when cultured with various broth media. The highest yield of cell mass, 2.31 g dry weight/50mL, was obtained on germinated-malt extract broth (GMEB). The optimal broth concentration which was measured hand refractometer for mycelium production was
. The optimal conditions estimated with response surface methodology under temperature, pH and incubation period were
, pH 5.72, 15.22 days, respectively, on GMEB having
concentration for mycelial production of A. mellea.
Studies on Artificial Cultivation of Pleurotus eryngii (De Canolle ex Fries) Quel.
Lee, Dae-Jin ; Kim, Kwang-Po ; Lee, Byung-Eui ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 192~199
DOI : 10.4489/KJM.2003.31.3.192
This study was carried out to investigate the artificial cultivation of Pleurotus eryngii on the optimal medium vessel, periods of cultivation and the optimal method of pinheading for both yield and quality of fruiting body were also performed. The optimal composition of sawdust medium in polypropylene(PP) bottle was combination of sawdust(70%) and corncob(30%) but increased amount of corncob delayed the period of mycelial growth. The mycelial growth and the yield of fruiting body in the medium with beat pulp were worse than that without beat pulp. The optimal composition of nutrients for both yield and quality of fruit body tuned out to be a combination of rice bran(12%), wheat bran(12%) and cottonseed cake(6%). Additions of zeolite, shell lime and bean curd dregs were not effective in mycelial growth and yield of fruit body. When testing size of PP bottle for cultivation, the larger of bottle mouth is, the more pinheading number found, but the number of available fruit body is not significantly different. The culture in
bottle is the best in the yield and quality of fruit body than those in
bottle. Using the PP bag for cultivation, a square shaped bag was better than a round shaped and black square shaped in mycelial growth and yield of fruit body. The most suitable period of incubation was 35 days after inoculation at
. When the incubation periods was decreased less than 35 days, the pileus formation and yields were very bad but a pinheading condition looked similar, For an optimal pinheading, turning upside down was better than standing and covering.
Studies on the Artificial Cultivation of Pleurotus cystidiosus O.K.Miller, the Abalone Mushroom
Jang, Kab-Yeul ; Jhune, Chang-Sung ; Shin, Chul-Woo ; Park, Jung-Sik ; Oh, Se-Jong ; Choi, Sun-Gyu ; Sung, Jae-Mo ;
The Korean Journal of Mycology, volume 31, issue 3, 2003, Pages 200~205
DOI : 10.4489/KJM.2003.31.3.200
The study was carried out on the artificial cultivation of the abalone mushroom, Pleurotus cystidiosus O.K.Miller. The pine sawdust substrates with 20% rise bran were good for mycelial growth and high quantity of P. cystidiosus in the bottle cultivation. Moreover, the proper volume for bottle cultivation was 850 ml and the removal of spawn and surface layer of the medium before pin-heading was more efficient. The yields of P. cystidiosus were higher in sawdust substrates added calcium carbonate than those not added calcium carbonate. The volume of 3 kg polypropylene bag is good for yield and biological efficiency in bag cultivation of P. cystidiosus. Cotton wastes were proper substrates for bag cultivation. In the effect of different cultivation temperature,
cultivation temperature was good for for primordial formation after inoculation.