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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 34, Issue 2 - Dec 2006
Volume 34, Issue 1 - Jun 2006
Selecting the target year
Distribution of Ectomycorrhizal Mushroom According to Altitude in NaeJangsan National Park
Jang, Seog-Ki ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 63~72
DOI : 10.4489/KJM.2006.34.2.063
This study was conducted to investigate the diversity of ectomycorrhizal mushroom by surveying sites from June 2004 to October 2005. The obtained results from investigation were as follows. The total of 5 classes 16 orders 63 families 149 genera and 358 species including saprophytic and ectomycorrhizal fungi was investigated. A total of 17 families 36 genera 152 species (1,285ea.) of ectomycorrhizal mushroom was investigated. The mushrooms are classified into 9 families 27 genera and 136 species in Agaricales, 5 families 6 genera and 12 species in Aphyllophorales and 3 families 3 genera and 4 species in Gasteromycetes. Dorminant species were Russulaceae (35 species) followed by Boletaceae (34 species), Amanitaceae(22 species) and Cortinariaceae (21 species). The mushroom occurrence of octomycorrhizal fungi was closely related to climatic conditions such as high air temperature and lots of rainfall from July to September. The environment factors which have a favorable influence of mushroom occurrence were soil pH, available
of soil and rainfall and air temperature of climatic environment.
Physiological and Genetic Changes by Mixing Culture of Shiitake
Lee, Bong-Hun ; Bak, Won-Chull ; Kim, Myung-Kil ; Ryu, Sun-Hwa ; Ryu, Sung-Ryul ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 73~78
DOI : 10.4489/KJM.2006.34.2.073
Attempts were made to investigate the physiological and genetic changes when two different shiitake (Lentinula edodes) strains are mixed. Mycelial growth of KFRI 180 strain and KFRI 1 strain were investigated 82 mm and 80 mm, respectively. Concerning the weight loss percentage of medium, KFRI 1 strain decreased 2.4% and KFRI 180 strain 1.6%. Plug-shaped spawn had no-problem to incubate and there were no differences among the ratios of mixture. Also, conditions of plug-shaped spawns were similar, When the isolated mycelia from plugshaped spawns was incubated again, KFRI 1 50%-KFRI 180 50% showed decreased growth of mycelia compared with other treatments. The same results were obtained from test tubes filled with sawdust. When surface of spawn bottles were observed, KFRI 1 50%-KFRI 180 50% showed spots, but other treatments were not different from KFRI 1 and KFRI 180. Test was made to confirm the strains by confrontation culture. The mixture of two strains was proved to be KFRI 1 regardless the ratios of mixture. However, by the RAPD primer analysis, when KFRI 1 was mixed with KFRI 180, KFRI 180 was stronger. Thus, the confrontation line on PDA was different from the bands analysis by primers. Attempts were made whether the fruit-bodies were made at the generating condition of spawn bottles. The results were that KFRI 1 100%, KFRI 1 90%-KFRI 180 10%, KFRI 1 80%-KFRI 180 20%, KFRI 1 50%-KFRI 180 50% treatment showed fruit-body formation. The shape of fruit-body was deformed, but the gill was made normally.
Comparison of Shiitake Productivity in Sawdust Media According to the use of Sawdust, Plug-shaped and Liquid Spawn
Lee, Bong-Hun ; Bak, Won-Chull ; Yoon, Kab-Hee ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 79~83
DOI : 10.4489/KJM.2006.34.2.079
This study was made to investigate the difference of the shiitake productivity according to the use of sawdust, plug-shaped and liquid spawn. Contamination of medium was confirmed by eye. There was no contamination in the liquid spawn treatment. And, contamination rates in the sawdust spawn and plug-shaped spawn were 6.7%, respectively. Productivity of fruit-bodies was 725.1 g from sawdust spawn, 726.2 g from plug-shaped spawn and 573.9 g from liquid spawn until 3rd flushing of fruit-bodies. The number of fruit-bodies was 57.4 per medium of sawdust spawn, 64.3 plug-shaped spawn and 45.1 liquid spawn. At yield per medium, the percentage of media producing over 1,000 g was 13% in plug-shaped spawn and 7% in liquid spawn. However, there was no medium producing over 1,000 g of fruit-bodies in sawdust spawn. Fruit-bodies of
were yielded from 79% of sawdust spawn treatment, 53% plug-shaped spawn and 33% liquid spawn treatment. When compared the productivity of fruit-bodies, liquid spawn treatment appeared lower than sawdust spawn and plug-shaped spawn treatment at 1st flushing, but appeared higher than the others at 2nd and 3rd flushing.
Biosynthesis of Organic Germanium Using Cordyceps militaris
Kim, Seung ; Kim, Jae-Sung ; Sapkota, Kumar ; Choi, Bong-Suk ; Park, Se-Eun ; Park, Yeal ; Chun, Hong-Sung ; Yoo, Jin-Cheol ; Choi, Han-Seok ; Kim, Myung-Kon ; Kim, Sung-Jun ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 84~87
DOI : 10.4489/KJM.2006.34.2.084
In the present study, an attempt has been made to produce a high quality medicinal mushroom Cordycops militaris supplemented with organic Ge. Cordycops militaris was cultivated in SDAY liquid medium containing yeast extract 10 g, peptone 10 g, glucose 40 g per liter and chrysalis media supplemented with inorganic Ge at 100, 500, 1000, and 5000 ppm concentrations. The greatest organic Ge production of 442.4 ppm/g and 284 ppm/g were observed in SDAY liquid and Chrysalis media cultures supplemented with 100 ppm inorganic Ge respectively. Similarly, 4,509.7 ppm/g and 1,058 ppm/g of organic Ge were obtained from liquid and chrysalis media cultures supplemented with 5,000 ppm and 1,000 ppm inorganic Ge, respectively. In addition, higher concentration of organic Ge was obtained in mycelia than fruiting bodies. These results indicate that the concentration of organic Ge increase with decreasing inorganic Ge concentration in the medium. This is the first report on production of high valuable Cordyceps militaris contained with organic Ge.
Investigation of Harmful Microorganisms and Physiological Characteristics of Mycelia in the Bed-log of Shiitake Strains Showing Low Fruit-body Productivity
Bak, Won-Chull ; Lee, Bong-Hun ; Ryu, Sung-Ryul ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 88~91
DOI : 10.4489/KJM.2006.34.2.088
Attempts were made to investigate the conditions of mycelia of one low-temperature type strain and one high-temperature type strain of shiitake showing poor fruit-body formation in bed-log, and to survey harmful microorganisms formed on the log surface in Gapyung County, Korea. When tested the growing ability of mycelia, the low-temperature type strain showed ca. 1.1% decrease compared with preserved original strain. And, the high-temperature type one showed ca. 8.0% decrease. The growth of isolated mycelia was tested in sawdust medium. The high-temperature type strain showed ca. 10.8% decrease compared with original strain, and the low-temperature type one showed ca. 25.1% decrease. Weight reduction rate was investigated. The high-temperature strain showed ca. 20.1% decrease and the low-temperature one ca. 19.0%. When compared with non-treatment, original high-temperature type strain showed 107.0% decrease, the isolated high-temperature type strain 49.5%, original low-temperature type one 85.4%, isolated low-temperature type one 50.0%. As the results of confrontation culture, the high-temperature type strain and the low-temperature type one were same as the original ones, respectively. And, in the bed-logs, Hypoxylon truncatum, Coriolus versicolor, Inonotus xeranticus, Daedaleopsis tricolor, Graphostroma platystoma, two species of Myxomycetes, Trichoderma sp. Hypoxylon fragiforme, H. howeianum, and Nitschkia confertula were observed as harmful microorganisms, and the bed-logs were not in good condition.
Antimicrobial Activity of the Coriolus versicolor Liquid Culture Extracts Against Antibiotic Resistant Bacteria and Purification of Active Substance
Lee, Jung-Sun ; Kim, Taeg ; Lee, Yoon-Hi ; Jin, Cheng-Min ; Kim, Hyun-Guell ; Kim, Woo-Jung ; Oh, Duek-Chul ; Park, Yong-Il ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 92~97
DOI : 10.4489/KJM.2006.34.2.092
The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.
Inhibitive Effect of Mouse Sarcoma 180 by Crude Polysaccharide Extracted from Fruiting Body of Aramillaria mellea
Kim, Sang-Beom ; Lee, Gun-Woo ; Kim, Hye-Young ; Shim, Mi-Ja ; Rho, Hyun-Su ; Lee, Hyun-Sook ; Lee, Min-Woong ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 98~104
DOI : 10.4489/KJM.2006.34.2.098
Armillaria mellea, one of edible and medicinal mushroom belonging to Tricholomataceae of Basid-iomycota, has been known to have outstanding inhibitive effects on the sarcoma 180 and Erhrlich carcinoma of mice. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr, HW and Fr. MeOH, respectively) were extracted from the mushroom. In vitro cytotoxicity tests, crude polysaccharide were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of
. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of
in mice inoculated with Sarcoma 180, respectively. Fr, NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by
folds, respectively. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 10 and 2 folds, respectively.
The Immuno-Modulatory and Antitumor Effects of Crude Polysaccharides Extracted from Tremella fuciformis
Oh, Yun-Hee ; Kim, Sang-Beom ; Lee, Gun-Woo ; Kim, Hye-Young ; Shim, Mi-Ja ; Rho, Hyun-Su ; Lee, Hyun-Sook ; Lee, Min-Woong ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 105~111
DOI : 10.4489/KJM.2006.34.2.105
Tremella fuciformis, one of edible and medicinal mushroom belonging to Tremellaceae of Basidiomycota, has been known to have a curative effect on sarcoma 180 of mice and lowering high blood pressure of human beings. Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) substances were extracted from Tremella fuciformis. In vitro cytotoxicity tests, Fr. HW and Fr. NaCl were not cytotoxic against cancer cell lines such as NIH3T3, Sarcoma 180, and HT-29 at the concentration of
, while Fr. MeOH was cytotoxic to NIH3T3 and Sarcoma 180. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 53% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by
folds, respectively. Intraperitoneal injection with Fr. NaCl increased the numbers of peritoneal exudated cells and circulating leukocytes by 7.4 folds and 1.6 folds, respectively, than in the control group. The antitumor effect of T. fuciformis against Sarcoma 180 of mice was likely due to immunopotentiation activity.
Selection of Lecanicillium Strains for Aphid (Myzus persicae) Control
Jung, Hye-Sook ; Lee, Hyang-Burm ; Kim, Keun ; Lee, Eun-Young ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 112~118
DOI : 10.4489/KJM.2006.34.2.112
To select efficient entomopathogenic fungal strains of Lecanicillium for the biocontrol of aphid, Myzus persicae, conidial suspension (
) was sprayed onto a detached Chinese cabbage leaf in a petri dish with a dampened filter paper that had 20 nymphs of aphid. Lecancillium strain 4078 and 6543 were the best strains for the biocontrol of aphid at high temperature of
and low relative humidity (RH) of 85%, respectively. The cumulative mortality of strain 4078 at
after 3 days was 100% and that of strain 6543 was 90% at 85% RH after 5 days. Strain 4078 also exhibited almost 100% germination ratio of conidia and high rate of mycelial growth at the broad temperature-range of
. The strain 4078 and 6543 were all identified as Lecanicillium species based on the DNA sequences (accession no.: EF026004 and EF026005, respectively) of the ITS regions of the fungi. Excellent production of aerial conidia of strain 6543 was accomplished by using steamed polished rice as the solid culture medium.
Antifungal Activity of Chitosans on Candida albicans and Trichophyton rubrum and its Induction of Apoptosis
Chee, Hee-Youn ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 119~121
DOI : 10.4489/KJM.2006.34.2.119
The antifungal activity of chitosan (
) and chitooligosaccharide (
) was investigated against Candida albicans and Trichophyton rubrum. Chitosan showed antifungal activity against C. albicans and T. rubrum at 50 and 100 ng/ml, respectively while chitooligosaccharide did not suppress the growth of fungus. The mode of antifungal activity of chitosan was found to be fungicidal activity. In order to investigate the induction of apoptosis by chitosan, exposure of phosphatidylserine on the surface of the cytoplasmic membrane was observed by the FITC-annexin V reaction. The results showed that chitosan induced apoptosis on C. albicans.
Occurrence of Gray Mold of Stachys sieboldii Caused by Botrytis cinerea
Kwon, Jin-Hyeuk ; Jin, Young-Min ; Bae, Sung-Mun ; Jeong, Eun-Ho ; Ryu, Jae-San ; Kim, Min-Keun ;
The Korean Journal of Mycology, volume 34, issue 2, 2006, Pages 122~124
DOI : 10.4489/KJM.2006.34.2.122
In March of 2005, gray mold disease caused by Botrytis cinereu on Stachys sieboldii Miq. was occurred in the mud cellar storage of Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The symptoms started with water-soaked and rotten in the tubers. The conidia were one celled and mostly ellipsoid or ovoid in shape and light gray in color. The conidia were
in size and the conidiophores were
in length. The pathogen formed conidia and sclerotia abundantly on PDA. The optimal temperature for mycelial growth and sclelotia formation was
. On the basis of symptom, mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries. This is the first report on gray mold of S. sieboldii caused by B. cinerea in Korea.