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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 36, Issue 2 - Dec 2008
Volume 36, Issue 1 - Jun 2008
Selecting the target year
Isolation and Identification of Filamentous Fungi from Indoor Air of a Sogokju Traditional Rice Wine Factory
Park, Ji-Eun ; Jeon, Young-Jae ; Kim, Ji-Hye ; Kim, Seong-Hwan ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 1~8
DOI : 10.4489/KJM.2008.36.1.001
To investigate the mycoflora of indoor air in a Sogokju, traditional rice wine, factory, fungi were sampled and analyzed from the air of several rooms in the factory using an Anderson air sampler and from two kinds of Nuruk. Twelve fungal species belonging to the genera of Aspergillus, Penicillium, Gibberella, Cladosporium, and Talaromyces were isolated. Species belonging to Aspergillus and Penicillium genera were the major species. Seven different species of Penicillium were isolated from each different room of the factory. The Aspergillus species found from indoor air of the factory was also found from Nuruk. Rhizopus sp. was commonly isolated from Nuruk but not from indoor air of the factory. This is first report of fungi present in indoor air of a traditional rice wine factory in Korea.
Development of Simple Colorimetric Method for Detecting Contamination of Liquid Spawn of Oyster Mushroom by pH Indicator
Jang, Myoung-Jun ; Lee, Yun-Hae ; Ju, Young-Cheol ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 9~15
DOI : 10.4489/KJM.2008.36.1.009
For the detection of contaminated liquid spawn, we selected suitable medium, indicator and developed method of diagnosis. The growth of pathogenic bacteria, Pseudomonas sp., and fungi, Trichoderma sp., in YPL media was better than in PDA and NA. In addition, the changes of color and absorbance of media were obviously showed when contaminated liquid spawn by pathogenic bacteria and fungi was incubated on YPL including phenol red for 48 hour at
. The color of YPLP after incubating of infected liquid spawn by Pseudomonas sp. and Trichoderma sp. were changed from orange to red and to scarlet, respectively. Whereas, the color of YPLP after incubation of only Pleurotus ostreatus indicated yellow at liquid spawn. Therefore, it is possible to easily distinguish contaminated liquid spawn by color of change in YPLP.
Cultural Characteristics of Cauliflower Mushroom, Sparassis crispa
Cheong, Jong-Chun ; Park, Jeong-Sik ; Hong, In-Pyo ; Seok, Soon-Ja ; Jhune, Chang-Sung ; Lee, Chan-Jung ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 16~21
DOI : 10.4489/KJM.2008.36.1.016
This study was carried out to obtain the basic data for the mycelial growth conditions of cauliflower mushroom, Sparassis crispa. Twenty-one isolates were collected from domestic and abroad. The optimal temperature and pH for the mycelial growth of these isolates were
and pH 6.0, respectively. The mycelial growth was the best in the HBA medium, but very poor in the Lilly medium. However no mycelial growth in the CzapekDox medium. The utilization of carbon source was the best with fructose, and that of nitrogen source was the best with glutamine when compared to other tested sources. The selected isolate ASI150010 produced the highest mycelial weight in liquid culture containing soybean mill (
) and potato (
) extract. And uncleaned rice, wheat and barley were found to be good substrates for the mycelial growth S. crispa.
Effects of Addition of Crab Shell to Sawdust Substrate on the Growth and Development of Pleurotus eryngii
Jo, Woo-Sik ; Rew, Young-Hyun ; Kim, Jong-Su ; Park, So-Deuk ; Seok, Soon-Ja ; Jung, Hee-Young ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 22~25
DOI : 10.4489/KJM.2008.36.1.022
Pleurotus eryngii is by far the best tasting oyster mushroom, well deserving of the title, the King Oyster. Popular in Europe, this stout, thickly fleshed mushroom, is one of the largest species in the genus. The effects of addition of crab shell to sawdust substrate for the growth of P. eryngii were investigated. Dried crab shell used in this study contained 55.2% C, 3.74% N, 5.57%
, 6.44% Ca, and pH 6.9. The addition of 1% crab shell (v/v) increased the yield of the mushroom fruit-body by 34% comparing control (sawdust 80, rice bran 20; v/v), 0.03% CaO content of 1% crab shell treatment was higher than 0.01% CaO of control and period of primordia formation was similar regardless of the treatments.
Investigation on Favorable Substrate Formulation for Bag Cultivation of Grifola frondosa
Kim, Jeong-Han ; Choi, Jong-In ; Chi, Jeong-Hyun ; Won, Sun-Yee ; Seo, Geon-Sik ; Ju, Young-Cheoul ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 26~30
DOI : 10.4489/KJM.2008.36.1.026
This study was carried out to develop artificial culture method of Grifola frondosa in polypropylene bag. To find out the favorable substrate formulation of G. frondosa, physicochemical conditions were investigated. The T2 formulation (55 : 25 : 12 : 8) mixing ratio of oak sawdust, oak chip, dried bean-curd refuse and wheat bran showed the shortest time to complete the crop cycle and the highest yield (weight of fresh mushrooms harvested at maturity). Those physicochemical conditions were pH 4.3, 2.4% crude fat contents, 54.1% total carbon, 1.42% total nitrogen, 38.1 C/N ratio, 75.5% porosity and
bulk density. Among the physicochemical factors, pH, crude fat and total nitrogen may affect mushroom yield. Therefore, development of favorable substrate would be benefit to increase production efficiency of G. frondosa, mushrooms and be commercial potential.
Studies on Favorable Light Condition for Artificial Cultivation of Grifola frondosa
Chi, Jeong-Hyun ; Kim, Jeong-Han ; Won, Sun-Yee ; Seo, Geon-Sik ; Ju, Young-Cheoul ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 31~35
DOI : 10.4489/KJM.2008.36.1.031
To elucidate optimum light conditions for artificial cultivation of Grifola frondosa, the effects of light quality (blue, green, white) and light intensity (200, 500, 800, 1200 lux) on primordium formation, morphological properties and yield of fruiting bodies of G. frondosa using bag cultivation method were tested. Among three light sources, white light source (
) had a higher mushroom yield (242 g/bag) and a shorter cultivation period (52 days) than those of the others. In particular, blue light source (
) induced the morphology of wide and deep colored pilei in G. frondosa fruiting body. The experimental results on the appropriate light intensity indicated that 500 lux light was the most effective on mushroom production, whereas primordium formation was effective at 200 lux.
Comparison of Productivity According to Sawdust Size and Effect of Additives for Sawdust Cultivation of Shiitake
Lee, Bong-Hun ; Bak, Won-Chull ; Ka, Kang-Hyeon ; Ryu, Sung-Ryul ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 36~40
DOI : 10.4489/KJM.2008.36.1.036
Studies were processed to confirm the differences of productivity according to sawdust size and effect of additives for sawdust cultivation of shiitake. In results of investigation until shiitake mycelia fully spread on surface of sawdust medium, mycelial growth in treatment of sawdust size
, and treatment of sawdust sizw
was the fastest. And, in investigation of rate of weight reduction, treatment of sawdust size below 1 mm without
was the highest. The tendency of fruiting was somewhat different according to treatments. In case of sawdust size below 1 mm with
, yield produced from 1st to 2nd flushing period was over 80%. However, in case of sawdust size below 1 mm plus
, yield produced from 3rd to 4th flushing period was more than it from 1st to 2nd flushing period and rate of yield produced from 1st to 2nd flushing period to total yield was 34%. Results of investigation on productivity, yield and the number of fruit-bodies were remarkably different according to treatments. Sawdust size
, whose total yield and the number of fruit-bodies were 568 g and 67, respectively, was the highest. And, total yield of medium of sawdust size below 1 mm plus
was lowest as 227 g and the number of fruit-bodies of medium of sawdust size below 1 mm plus
was lowest as 24. In investigation on amount of fruit-bodies over 10 g, medium of sawdust size
was highest as 397 g.
Comparison of Productivity according to Different Quantity of Sawdust and Liquid Spawn for Sawdust Cultivation of Shiitake
Bak, Won-Chull ; Lee, Bong-Hun ; Ka, Kang-Hyeon ; Ryu, Sung-Ryul ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 41~44
DOI : 10.4489/KJM.2008.36.1.041
Studies were made to investigate the difference of the shiitake productivity according to the use of sawdust and liquid spawn on middle-temperature type strain. Treatments inoculated with sawdust spawn have shown that yields were dispersed according to flushing periods and fruit-bodies occurred sporadically on medium. However, treatments inoculated with liquid spawn have shown that yield was relatively concentrated at 1st flushing period and fruit-bodies were densely occurred on medium. At total yield and the number of fresh fruit-bodies until seventh flushing, treatments inoculated with liquid spawn were more than those inoculated with sawdust spawn. Also, biological efficiency (B.E.) of inoculation with 20 ml liquid spawn which produced 30% of the medium weight was highest as 69%, but biological efficiency of inoculation with 10 g sawdust spawn was lowest as 42%. And, although rate of fruit-bodies over 10 g in total yield was high on treatments inoculated with sawdust spawn, amounts of fruit-bodies over 10 g were not different among treatments.
Eeffect of Wood Xylem Flour in Liquid Culture on Mycelial Biomass of Lentinus lepideus and Lentinus edodes
Lee, Wi-Young ; Ahn, Jin-Kwon ; Park, Eung-Jun ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 45~50
DOI : 10.4489/KJM.2008.36.1.045
This study was carried out to investigate the promoting effect of wood flour on the mycelial growth of Lentinus lepideus and Lentinus edodes. To determine the optimal culture condition, we first examined the tissue origin of pine flour (Pinus densiflora) including needle, bark, root and xylem. Only the xylem-derived flour increased mycelial growth compared to no treatment control. The addition of the xylem flour (5 g/l) showed the highest increase and the glucose level in the basal medium was best at 10 g/l. The smaller particle size of the xylem flour showed the positive effect on mycelial growth; two-fold increase when supplemented with flour of which particle size is less than
in diameter compared to
. The addition of the xylem flour continuously increased the mycelial production for 25 days while mycelia stopped growing within 15 days without the xylem flour. In addition, when woody flour obtained from the different tree species was applied to L. edodes mycelial culture, all treatments accelerated mycelial production compared to the control. Based on all results described above, we conclude that the supplementation of woody flour to culture medium may be an another promising way to increase mycelial production of economically important fungi.
Studies on the Morphological Characteristics of Coprinus species
Kim, Yong-Gyun ; Kim, Hong-Kyu ; Lee, Byung-Joo ; Yang, Euy-Seog ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 51~57
DOI : 10.4489/KJM.2008.36.1.051
To develop artificial cultivation and improve some problems such as auto-lysis on commercialization of Coprinus comatus that has been known edible and medicinal mushroom, they were conducted for selection of superior strain, suitable culture methods for mycelial growth and fruiting, and morphological characteristics of fruit body. Strain CM 980301 of Coprinus comatus was selected as a superior strain for artificial cultivation. Wheat grain and rice straw full-grown compost media were most effective for preparation of spawn and artificial cultivation of C. comatus, respectively. Spawn running of Coprinus spp. on the rice straw full-grown compost media required to be 15 days from 24 to
. The casing layer incubation before initiation of fruit body formation, required for 13 days at same temperature for spawn running. And then require
days for initiation and
days for development of fruit body from 20 to
. The fruit body of strain CM 980301 was harvested within a week from initiation of primordium formation. The hardness of pileus and stipe that were harvested in optimal stage showed 102 to 169, and 128 to
, respectively. Yields of srain CM 980301 from the rice straw full-grown compost media was
. Weight of individual fruit body was 17.9 g in average.
Characteristics of Mycelial Growth and Fruit Body Development of White Pleurotus ostreatus 'Miso'
Kim, Hong-Kyu ; Kim, Yong-Gyun ; Lee, Byung-Joo ; Lee, Ka-Soon ; Yang, Eyu-Seog ; Park, Myung-Soo ; Yoo, Young-Bok ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 58~62
DOI : 10.4489/KJM.2008.36.1.058
'Miso', a new variety of oyster mushroom was developed for the bottle culture at the Chungnam Agricultural Research & Extension Services. Its mycelium grew rapidly with
on PDA medium. The optimum pH of the mycelial growth was pH 5.0. It took 24 days for the primitive primordium formation after inoculation on pine sawdust media mixed with 20% wheat bran. Fruiting body color was white, and the shape of pileus was convex-umbonate. In the bottle culture, the yield was 115.7 g per 850 ml bottle. Stipe length was about 54 mm, the number of stipe per bottle was 18.1, the size of pileus was 28 mm, and gill was crowded. The moisture content of 'Miso' was lower than that of Jangan No.5 and Wonhyeong No.1, and contents of protein, ash and sugar of 'Miso' were higher than those of other varieties, and tannin acid content of 'Miso' was lower than that of others. On the basis of AFLP analysis, the 'Miso' was distinct not only from Wonhyeong No.1, but also from their closest relative, oyster mushrooms.
Confirmation of Genetic Homogenecity among Wild Strains of Piptoporus betulinus Collected from a Single Tree
Ka, Kang-Hyeon ; Chang, Ji-Youn ; Ryu, Sung-Ryul ; Yoon, Kab-Hee ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 63~65
DOI : 10.4489/KJM.2008.36.1.063
Four strains of Piptoporus betulinus were isolated from a trunk of the Betula ermani in Seorak mountain. These strains were studied to observe whether they are originated from the same genet or not, by using the method of pairing culture between strains and by comparing part of the nuclear rDNA of ITS regions amplified with NSI1 and ITS4B primers. The demarcation line was not formed on the potato dextrose agar plates in the combinations of two different strains. Partial nuclear rDNA sequences of the four strains were also the same. As a result, it is considered in high probability that they are derived from a single spawn.
Antitumor and Immuno-modulatory Effect of Crude Polysaccharides from Fruiting Body of Tremella aurantialba Against Mouse Sarcoma 180
Lee, Geon-Woo ; Kim, Hye-Young ; Hur, Hyun ; Lee, Min-Woong ; Shim, Mi-Ja ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 66~74
DOI : 10.4489/KJM.2008.36.1.066
Tremella aurantialba, one of edible and medicinal mushrooms belonging to Tremellaceae of Basidiomycota, has been known to have outstanding curing effects on coughing, tracheitis and hypertension of humans and antitumor activity on the sarcoma 180 and Erhrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests showed that all crude polysaccharides extracted from the fruiting body were not cytotoxic against cancer cell lines such as RAW 309 CR.1 and Sarcoma 180 at the concentration of
. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of
in mice inoculated with Sarcoma 180. Fr. MeOH improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by 1.16 folds at the concentration of
. In case of Fr. HW, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 1.42 and 2.87 folds, respectively.
Antitumor and Immuno-potentiating activity against Mouse Sarcoma 180 by Crude Polysaccharides from Fruiting Body of Lentinus giganteus
Lee, Geon-Woo ; Kim, Hye-Young ; Hur, Hyun ; Lee, Tae-Soo ; Lee, U-Youn ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 75~83
DOI : 10.4489/KJM.2008.36.1.075
Lentinus giganteus, one of edible and medicinal mushroom belongs to Pleurotaceae of Agaricales, has been known to contain some inhibitive substances on Sarcoma 180 and curative effect on high blood pressure. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180 and HepG2 at the concentration of
, but crude polysaccharides from Fr. NaCl was toxic to NIH3T3 at the concentration of
. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of
in mice previously inoculated with Sarcoma 180, respectively. Fr. NaCl exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by
folds compared with control at the concentration of
, respectively. The numbers of peritoneal exudate cells and circulating leukocytes were increased by 7.7 and 1.6 folds by injecting Fr. NaCl and Fr. MeOH into the mice at the concentration of 50 mg/ml body weight, respectively.
Immuno-potentiating and Antitumor Effects against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Russula rosacea
Choi, Yon-Il ; Lee, Geon-Woo ; Hur, Hyun ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 84~92
DOI : 10.4489/KJM.2008.36.1.084
Russula rosacea, one of edible and medicinal mushroom belonging Agaricales of Basidiomycota, has been known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at the concentration of
. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of
in mice previously inoculated with Sarcoma 180. Fr. HW improved the immuno-potentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by 6.8 fold compared with control at the concentration of
. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6 and 2.6 folds at the concentration of 50 mg/kg, respectively. Therefore, the antitumor effect against mice Sarcoma 180 by Russula rosacea could be due to immunomodulating activity.
Expression and Cloning of Microbial Transglutaminase in S. cerevisiae
Kim, Hyoun-Young ; Oh, Dong-Soon ; Kim, Jong-Hwa ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 93~97
DOI : 10.4489/KJM.2008.36.1.093
microbial transglutaminase (mTGase) from the actinomycete Streptomyces mobaraensis IFO13819 is a useful enzyme in the food industry. It is consists 406 amino acid residues, which comprised leader and pro region of 75 amino acid residues and the structure region of 331 amino acid residues. Pro and structure gene of TGase were cloned into the yeast shuttle vector pYAEG-TER and then used to transform Saccharomyces cerevisiae 2805. Expression of mTGase in recombinant was confirmed with Northern hybridization and the maximal activity of TGase was shown 26 mU/ml.
Log Cultivation of the Birch Fungus Piptoporus betulinus using Betula davurica
Ka, Kang-Hyeon ; Ryu, Sung-Ryul ; Lee, Bong-Hun ; Yoon, Kab-Hee ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 36, issue 1, 2008, Pages 98~100
DOI : 10.4489/KJM.2008.36.1.098
A birch fungus, Piptoporus betulinus, is judged the mushroom with economical value that produces the fruiting body on Betula trees, but the mushroom rarely occurs in Korean birch forest. So, the fungus was applied to log cultivation for the production of fruiting body on Betula davurica. The fungus produce the fruiting bodies in the early August of the next year after inoculation in April and its mushroom matured in October. The mushroom produced 212 g to 1,298 g fresh weight in one or two mushrooms per a log. Thus, it is considered that log cultivation of the fungus has a potential to mass production of fruiting body and is expected to introduce to more researches to develop the use of this mushroom.