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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 38, Issue 2 - Dec 2010
Volume 38, Issue 1 - Jun 2010
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Characteristic of Orchid Mycorrhizal Fungi from Roots of Cypripedium japonicum and C. macranthum
Sim, Mi-Yeong ; Youm, Jae-Young ; Chung, Jae-Min ; Lee, Byung-Chun ; Koo, Chang-Duck ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 1~4
DOI : 10.4489/KJM.2010.38.1.001
Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.
Fruit-body Production of Auricularia auricula-judae by Sawdust Cultivation
Jo, Woo-Sik ; Hwang, Eok-Keum ; Kang, Min-Jin ; Choi, Seong-Yong ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 5~7
DOI : 10.4489/KJM.2010.38.1.005
Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. Present experiments were conducted to determine the possibility of artificial cultivation with oak sawdust of A. auricula-judae. The duration of mycelial growth and days of pinhead formation on oak sawdust bag (1.4 kg) were 29~32 days and 13~17 days, respectively. The yield of fresh fruit-body was 275~350 g.
Ecological Study on Wild Mushrooms at the Dongbaekdongsan of Seunheulgot in Jeju Island
Ko, Pyung-Yeol ; Seok, Soon-Ja ; Jeun, Yong-Chull ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 8~15
DOI : 10.4489/KJM.2010.38.1.008
To understand fungal species in evergreen broadleaf forest, occurrence and habitate of wild mushrooms spontaneously growing at the Dongbaekdongsan of Seonheulgot in Jeju Island were investigated. Fungal samples were collected from an area of
size consisting of four designated plots from in June 2007 to December 2008. Wild mushrooms were identified 69 genera and 178 species within the sampling places. Among 178 species, twenty-eight genera and fifty species were first reported in Jeju Island. Especially, Amanita pseudogemmata was first recorded in Korea. The mushroom species were divided into six groups on the basis of fungal habitats in which 44 species were on dead tree, 29 species on fallen leaf, 99 species on soil surface, 2 species on animal feces, 2 species on insect or mushroom, and 2 species on moss.
Mycelial Growth and Fairy-Ring Formation of Tricholoma matsutake from Matsutake-Infected Pine Trees
Ka, Kang-Hyeon ; Hur, Tae-Chul ; Park, Hyun ; Kim, Hee-Su ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 16~20
DOI : 10.4489/KJM.2010.38.1.016
Transplantation of matsutake-infected pine tree into a pine forest has great potential as an artificial cultivation method of Tricholoma matsutake. Matsutake-infected pine trees had previously transplanted by this research group into the experimental area from 2001 to 2004 and its survival rate determined in 2006 was 20%. For the survived samples, the characteristics of mycelial growth and the development of fairy-ring formation of T. matsutake have been evaluated until 2009. It was found that size of the indeterminate ring showed significant differences among the individual trees and varied from
. The variation was possibly resulted from the differences in production area of the matsutake-infected pine tree and those in site characteristics of the transplanted spots. For the characteristics of mycelial growth, it grew in the shape of a cudgel or an acute-angled `V` in early stage, and then the shape became more wider and changed into an obtuse-angled `V` as time passed. We expect that matsutake mushrooms may occur from the fairy-rings of some of these matsutake-infected pine trees in the autumn of 2010.
Selection of High Efficient Enzyme for Protoplasts Isolation from Mushrooms
Kim, Jong-Kun ; Kim, Jin-Hee ; Kong, Won-Sik ; Kang, Hee-Wan ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 21~24
DOI : 10.4489/KJM.2010.38.1.021
This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enzymes. The highest yield of protoplasts was obtained from the homogenized mycelia treated with the enzyme combination of
200G and cellulase onozuka R-10. The protocol was also available for Pleurotus ostreatus, P. eryngii, and Hypsizygus marmoreus.
Characteristics of New Shiitake Strain "Sanlim No. 7" Produced by Di-mon Hybridization Method
Bak, Won-Chull ; Lee, Bong-Hun ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 25~28
DOI : 10.4489/KJM.2010.38.1.025
New shiitake(Lentinula edodes) strain "Sanlim No. 7" was produced by Di-mon hybridization method. Bed-log cultivation of Sanlim No. 7 was performed. Fruit-body production was most at autumn and it is suitable to use as fresh shiitake rather than dried one. The fruit body is hemispherical shape, brown coloured and diameter of pileus is ca. 47 mm. Row of gill is straight, and its width is narrow. Optimum temperature of fruit-body formation was
, and the fruiting is sporadic. The total amount of fruit-body production during 4 years(one generation) was ca. 1.4 kg/log.
Effect of KGD1 Deletion on Cell Wall Biogenesis in Saccharomyces cerevisiae
Kim, Sung-Woo ; Ahn, Ki-Woong ; Park, Yun-Hee ; Park, Hee-Moon ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 29~33
DOI : 10.4489/KJM.2010.38.1.029
KGD1 gene was cloned by functional complementation of defects in
-1,3-glucan synthase activity of the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. We performed the gene disruption experiment to characterize the function of KGD1 gene, which encodes
-ketoglutarate dehydrogenase, in cell wall biosynthesis. The disruption of KGD1 showed the decreased growth rate, the increase of chitin synthases activity, alterations in cell wall composition, and increase of susceptibility to cell wall inhibitors such as Calcofluor white and Nikkomycin Z. These results suggested that KGD1 might be involved in cell wall biogenesis, especially the biosynthesis of
-1,6-glucan and chitin in S. cerevisaie.
Molecular Genetic Classification of Hypsizigus marmoreus and Development of Strain-specific DNA Markers
Lim, Yun-Jeong ; Lee, Chang-Yun ; Park, Jeong-Eun ; Kim, Sang-Woo ; Lee, Hyun-Sook ; Ro, Hyeon-Su ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 34~39
DOI : 10.4489/KJM.2010.38.1.034
We have attempted to verify 30 strains of Hypsizigus marmoreus from various mushroom stocks in Korea using random amplified polymorphic DNA (RAPD) methodology. Chromosomal DNAs of them were extracted and subjected to PCR analyses with 3 random primers. Each PCR produced approximately 30 distinct PCR bands with the size from 200 bp to 3000 bp. A dendrogram was acquired using the unweighted pair-group method with arithmetic average (UPGMA) clustering methodology on the basis of the DNA band pattern. The analysis revealed that 30 strains of H. marmoreus were clustered into two distinct clusters. Cluster 1 contained 3 subgroups while the cluster 2 consisted of rather diverse strains. Interestingly, Hm3-10, a wild strain collected from Deog-Yu mountain, was not included in either clusters, indicative of uniqueness of this strain. We nextly attempted to develop strain-specific DNA markers to verify a specific strain. A unique band in the RAPD gel lane of Hm0-4 was extracted and its sequence was determined. PCR with a primer set from the determined sequence revealed that the primer set gave a 250 bp DNA band only for Hm0-4, indicating that this approach works well for the strain-specific identification of H. marmoreus.
Multi-locus Phylogeny Analysis of Korean Isolates of Phytophthora Species Based on Sequence of Ribosomal and Mitochondrial DNA
Seo, Mun-Won ; Song, Jeong-Young ; Kim, Hong-Gi ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 40~47
DOI : 10.4489/KJM.2010.38.1.040
To investigate genetic relationships either interspecies or intraspecies of 14 Korean Phytophthora species, sequence analyses of nuclear DNA (ypt gene and rDNA-IGS region) and mitochondrial DNA (Cox gene,
-tubuline gene, and EF1A gene) were performed. All of 14 Korean Phytophthora species clearly clustered into foreign isolates of each species. These Korean isolates in Phytophthora species also showed no correlation between molecular classification and morphological classification like as in case of foreigners. P. palmivora KACC 40167 reported previously from genetic groups of Phytophthora species in Korea was not consistent with the classification system, and therefore was required re-examination for the genetic group analysis. Korean isolates of P. drechsleri KACC 40195 showed very close relationship with P. cryptogea KACC 40161 above 94% bootstrap value in P. cryptogea-P. drechsleri complex group. Identification of these isolates is still unclear, because P. cryptogea and P. drechsleri were not differentiated in this study. On the other hand, it was required to unify species for these two species, since P. parasitica and P. nicotianae were clustered into a group on the level of 99 to 100% sequence homology. Comparing to the sequences of foreigners, Korean isolates were newly divided to ten groups in the phylogenic system. These results could be prepared useful informations to understand genetic diversity of Phytophthora species in Korea.
Agrobacterium-mediated transformation using gill tissue of Flammulina velutipes
Park, Soon-Young ; Van Peer, Arend F. ; Jang, Kab-Yeul ; Shin, Pyung-Gyun ; Park, Yun-Hung ; Yoo, Young-Bok ; Park, Ki-Moon ; Kong, Won-Sik ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 48~53
DOI : 10.4489/KJM.2010.38.1.048
Agrobacterium-mediated transformation was conducted in order to generate DNA insertional mutants of Flammulina velutipes. Agrobacterium tumefaciens AGL-1 harboring pBGgHg was transformed into gill tissues of Flammulina velutipes strain KACC42777. The transformants resistant on hygromycine (
) were confirmed by PCR. The targeted insertional sites were amplified by inverse PCR and sequenced. To find the phenotype variation of all generated transformants, bottle cultivation which followed by the standard cultivation protocol were conducted. Color variation was observed on the cultivated fruiting bodies. Furthermore, the transformant pool will be used as a good genetic resources for studying gene function.
Manufacture and Characteristics of Food Additives, Phellineus linteus Powder-containing Anti-obesity Lipase Inhibitor
Lee, Jong-Kug ; Jang, Jeong-Hoon ; Seo, Geon-Sik ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 54~56
DOI : 10.4489/KJM.2010.38.1.054
This study was performed to develop anti-obesity Phellineus linteus powder for application as food additives. P. linteus powders which contained anti-obesity lipase inhibitor were prepared by freeze drying and spray drying. Effects of various additives on the quality of their powders were investigated. 10% over addition of dextrin and gum-arabia was good in the rheological properties of their powders and optimal spray temperature was near
. When their powders were sealed and storaged at room temperature for 6 months, its characteristics were not changed. Especially, changes of its colors was repressed by addition of 0.3 M oxalic acid, citric acid and ascorbic acid than those by non-treatment.
Purification of Anti-obesity Lipase Inhibitor from the Fruiting Body of Phellinus linteus
Lee, Jong-Kuk ; Song, Jung-Hwa ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 57~61
DOI : 10.4489/KJM.2010.38.1.057
For development of anti-obesity nutraceuticals from mushrooms, new anti-obesity lipase inhibitor from Phellinus linteus was purified by systematic solvent extraction, TLC and HPLC and characterized. Methanol extract from P. linteus most effectively inhibited(72.5%) porcine pancreatic lipase and ethyl acetate fraction showed the highest inhibitory activity in the systematic solvent extraction. A lipase inhibitor from the ethyl acetate fraction was purified through following steps including 3 times silica gel chromatography and preparative HPLC. The purified lipase inhibitor was a yellowish geen powder and its
value was 175 ng. Its molecular weight by MALDI-TOF-MS was 523.06 Da and showed maximal absorption spectrum at 225.1 nm.
Antagonistic Properties of Mushroom Strains to Korean Oak Wilt Pathogen, Raffaelea quercus-mongolicae
Jeon, Sung-Min ; Ka, Kang-Hyeon ; Kim, Kyung-Hee ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 62~68
DOI : 10.4489/KJM.2010.38.1.062
This study was performed to investigate the antagonistic properties of mushroom strains against Korean oak wilt pathogen (Raffaelea quercus-mongolicae) by assessing their predominance on potato dextrose agar plates after pairing culture. Among the total of 158 strains of mushroom tested, thirty-three strains showed no antagonistic activities against the pathogen in both the short term (6~7 days) and long term (27~31 days). Ninety-six strains of tested mushrooms had a weak or no long term antagonistic activity. The antagonistic activities of 29 strains were higher than those of other strains with the levels of
R for short and long term, respectively. We found that these strains with antagonistic activities against R. quercus-mongolicae belong to the species of mushrooms living on different host plants such as conifers, oaks and other broad-leaved trees. In order to use the trees damaged by Korean oak wilt as a resource(bed-log) for cultivating mushrooms, seven strains of antagonist 29 strains were selected as follows; Laetiporus sulphureus KFRI 654, Laetiporus miniatus KFRI 914, Lentinula edodes KFRI 269 and 764, Pycnoporus coccineus KFRI 588, Schizophyllum commune KFRI 832 and Trametes versicolor KFRI 100.
Effect of Hydrophobicity of Oyster Mushroom on the Severity of Brown Blotch
Lee, Ki-Young ; Yang, Seung-Up ; Han, Jong-Woo ; Yoon, Kun-Sik ; Jang, Hoo-Bong ; Cha, Jae-Soon ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 69~74
DOI : 10.4489/KJM.2010.38.1.069
The 2 strains of Pleurotus ostreatus showing very severe and mild symptom of brown blotch were selected, and their F1 hybrid strains were made by mating of their single spore isolates. Hydrophobicity of fruiting body surface and brown blotch severity of the parental and their 11 F1 hybrid strains showing different level of severity of brown blotch were determined. Correlation coefficient (R) between disease severity and hydrophobicity were 0.68 and 0.70 on two independent experiments. Correlation coefficient between disease severity and disease incidence that was determined in the oyster mushroom farm were 0.46 and 0.52 on two independent experiments. When GFP-tagged Pseudomonas tolaasii was monitored with confocal microscope on cap surface of fruiting body, more cells of the pathogen were clustered on the severe strain than the mild strain, which indicates that the bacterial pathogen proliferates more on the severe strain. These results suggest that hydrophobicity of oyster mushroom fruiting body affects disease severity of the brown blotch disease; the longer the bacterial suspension stays on fruiting body surface more severe symptom of the blotch develops.
Fungicide Resistance of Gibberella fujikuroi Isolates Causing Rice Bakanae Disease and Their Progeny Isolates
Kim, Jung-Mi ; Hong, Sung-Kee ; Kim, Wan-Gyu ; Lee, Young-Kee ; Yu, Seung-Hun ; Choi, Hyo-Won ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 75~79
DOI : 10.4489/KJM.2010.38.1.075
A total of 25 isolates of Fusarium fujikuroi were obtained from diseased rice plants in Korea from 2006 to 2007 to assess their resistance against fungicides prochloraz and benomyl + thiram. Minimal inhibitory concentration (MIC) values of F. fujikuroi isolates were examined by agar dilution method. Most of the isolates were sensitive to the fungicides. Out of 25 isolates, six were resistant to prochloraz and three to benomyl + thiram. In addition, the isolates CF245, CF249 and CF337 showed resistant to both fungicides. The progenies (
isolates) obtained through two different crosses between sensitive parental isolates(CF202, CF232 and CF179) and resistant parental isolate (CF337) were evaluated for their mycelial growth at different temperatures and resistance against fungicides. Mycelial growth rate of
isolates originated from CF202
CF232 was similar to the parental isolates. However mycelial growth rate of
isolates originated from CF179
CF337 was faster than their parent isolates. In case of prochloraz, distribution ratio of sensitivity(S) to resistance(R) against to the fungicide of
isolates originated from CF202
CF232 and CF179
CF337 was 86 : 14 and 78 : 22, respectively. In case of benomyl+thiram, all the
isolates originated from CF202
CF232 were sensitive to the fungicide, however ratio of sensitivity(S) to resistance(R) against to the fungicide of
isolates originated from CF179
CF337 was 35 : 65.
Formation of Fruiting Body of Omphalotus japonicus by Sawdust Cultivation
Ka, Kang-Hyeon ; Park, Hyun ; Hur, Tae-Chul ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 80~82
DOI : 10.4489/KJM.2010.38.1.080
Omphalotus japonicus is known to as a poisonous mushroom in East Asia, but Illudin S, bioluminescent compound, has been recently reported for an anticancer effect. The fungus is quite rare in natural condition in Korea. Therefore, we tried to cultivate in the mushroom on the sawdust cultivation to provide sufficient amount of mushroom for pharmaceutical study for the future. The mushroom could be harvested from all sawdust media used in this study, and the sawdust of Pinus densiflora was considered to be the best for mushroom cultivation with considering mushroom productivity and cultivation period. The mushroom was produced 43~80 g fresh weight per 600 g sawdust medium in average, and could be harvested one to three times during cultivation period.
Fruit-body Production of Inonotus obliquus on Living Betula platyphylla var. japonica
Park, Hyun ; Ka, Kang-Hyeon ; Lee, Bong-Hun ; Ryu, Sung-Ryul ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 83~84
DOI : 10.4489/KJM.2010.38.1.083
We inoculated the spawn of Inonotus obliquus on living trees of Betula platyphylla var. japonica, and could get several fruiting bodies from the inoculations. The fruiting body showed almost the same feature of that collected from natural habitats in Korea, while the size was much smaller than that of natural one. The diameter of the fruiting body ranged from 1.1 cm to 4.8 cm, and the height 0.5 cm to 2.0 cm. We expect that a harvest is possible within 5 years, but need to check the growth rate of the fruiting body to decide suitable time for getting more economic value.
Screening of Anti-gout Xanthine Oxidase Inhibitor from Mushrooms
Zanabaatar, Bolormaa ; Song, Jung-Hwa ; Seo, Geon-Sik ; Noh, Hyung-Jun ; Yoo, Young-Bok ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 38, issue 1, 2010, Pages 85~87
DOI : 10.4489/KJM.2010.38.1.085
Anti-gout xanthine oxidase inhibitory activity of water extracts from various mushrooms were determined. The highest xanthine oxidase inhibitory activity was 72.9% in the water extract from fruiting body of Agaricus brazillensis and also were high in the extract from fruiting bodies of Pleurotus salmoneostramineus(60.1%), Phellinus baumii(57.7%), Agaricus bisporus(56.7%) and Hericium erinaceum(53.4%). The xanthine oxidase inhibitor was maximally extracted when Agaricus brazillensis fruiting body was treated with water at
for 24 h.