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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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The Korean Society of Mycology
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Volume & Issues
Volume 38, Issue 2 - Dec 2010
Volume 38, Issue 1 - Jun 2010
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The Roles of Protein Degradation During Fungal-plant Interactions
Ahn, Il-Pyung ; Park, Sang-Ryeol ; Bae, Shin-Chul ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 89~94
DOI : 10.4489/KJM.2010.38.2.089
Plant pathogenic fungi are the most diverse and drastic causal agents of crop diseases threatening stable food production all over the world. Plant have evolved efficient innate immune system to scout and counterattack fungal invasion and pathogenic fungi also developed virulence system to nullify plant resistance machinery or signaling pathways and to propagate and dominate within their niche. A growing body of evidences suggests that post translational modifications (PTMs) and selective/nonselective degradations of proteins involved in virulence expression of plant pathogenic fungi and plant defense machinery should play pivotal roles during the compatible and incompatible interactions. This review elucidates recent investigations about the effects of PTMs and protein degradations on host defense and fungal pathogens' invasions.
Notes on Rare Species of Mycoparasitic Forming Fungus in Korea
Seok, Soon-Ja ; Park, In-Cheol ; Kim, Yang-Sup ; Kim, Wan-Gyu ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 95~98
DOI : 10.4489/KJM.2010.38.2.095
One interesting parasitic forming fungus, being grown on the eggs of Phallus impudicus was collected from Mulchajoreum and on woods of Saryeoni in Jeju island. This taxon identified as a Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi (Hyporeaceae) based on morphological characteristics. According to the interpretation of Doi(1978), this taxon hsving the stipitate, genus Podostroma, family Hypocreaceae, Hypocreales, Ascomycota was identified as Podostroma solmsi f. octospora and its korean common name designated by authors. This species is characterized in having the fusiforme to ellipsoidal, unicellular and warted ascospores without septate and with blunt apiculus at each end and mycoparasitic habitat. Although there is some mycologists suggest that this taxon having above characters needs to be derived from Hypomyces. But authors are primarily going to record this species as a named "Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi" for the fungus flora of Korea. In a future, it is need in detail to compare their DNA sequencing data on the type species and some related species. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.
Isolation and Identification of Entomopathogenic Fungi Collected from Mountains and Islands in Korea
Kim, Wan-Gyu ; Seok, Soon-Ja ; Weon, Hang-Yun ; Lee, Kang-Hyo ; Lee, Chan-Jung ; Kim, Yang-Sup ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 99~104
DOI : 10.4489/KJM.2010.38.2.099
Dead insects infected with fungi were collected from mountains and islands in several locations in Korea from 2003 to 2007. Morphological characteristics of the fungal fruiting bodies produced on the insect samples were examined. A total of 542 entomopathogenic fungal isolates were obtained from the insect samples and identified based on their morphological and cultural characteristics. Three species of Beauveria, eight species of Cordyceps and four species of Isaria were mainly isolated from the insect samples. The other entomopathogenic fungi isolated from the insect samples were Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp. and Verticillium sp.
Genetic Diversity and Phylogenetic Relationship in Korean Strains of Lentinus lepideus Based on PCR Polymorphism
Lee, Jae-Seong ; Cho, Hae-Jin ; Yoon, Ki-Nam ; Alam, Nuhu ; Lee, Kyung-Lim ; Shim, Mi-Ja ; Lee, Min-Woong ; Lee, Yun-Hae ; Jang, Myoung-Jun ; Ju, Young-Chul ; Cheong, Jong-Chun ; Shin, Pyung-Gyun ; Yoo, Young-Bok ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 105~111
DOI : 10.4489/KJM.2010.38.2.105
Lentinus lepideus, known as train wrecker fungus, has been used for nutritional and medicinal purposes. Recently, commercial cultivation technique and a new cultivar of the mushroom were developed. To investigate the genetic diversity and phylogenetic relationship for identifying the mushroom strains and cultivar, one commercial and 13 strains of Lentinus lepideus from different geographical regions of Korea were analyzed by ITS regions of rDNA and RAPD of genomic DNA. Three strains of Lentinus edodes were also used for the analysis. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 173 to 179 bp and 203 to 205 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical with 156 base pairs. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into four clusters, while 3 strains of L. edodes was divided into a new cluster. Ten primers out of 20 arbitrary primers used in the RAPD-PCR efficiently amplified the genomic DNA. The numbers of amplified DNA bands varied with the primers and strains, with polymorphic DNA fragments in the range from 0.2 to 2.6 kb. The results showed that phylogenetic relationship among Korean strains of Lentnus lepideus is high, but genetic diversity is low.
Comparative Molecular Phylogenetic Relationships in Different Strains of Pleurotus spp.
Cho, Hae-Jin ; Lee, Jae-Seong ; Yoon, Ki-Nam ; Alam, Nuhu ; Lee, Kyung-Lim ; Shim, Mi-Ja ; Lee, Min-Woong ; Cheong, Jong-Chun ; Shin, Pyung-Gyun ; Yoo, Young-Bok ; Lee, U-Youn ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 112~119
DOI : 10.4489/KJM.2010.38.2.112
Pleurotus spp. have been used for edible and medicinal purposes in Asian countries for a long time. The fruiting bodies of the Pleurotus ostreatus, Pleurotus citrinopileatus and Pleurotus salmoneostramineus contained many physiologically beneficial substances for human health. Therefore, it is necessary to study the genetic diversity of Pleurotus mushroom cultivars commercially cultivated in Korea. Eleven strains of Pleurotus spp. were collected from different geographical regions in South-East Asia and ITS regions of rDNA and RAPD of genomic DNA were analyzed. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 167 to 254 bp and 156 to 213 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into 4 clusters. Eleven Pleurotus species were also analyzed by RAPD with 20 arbitrary primers. Ten of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.1 to 2.0kb. The results revealed that genetic diversity of selected strains of P. ostreatus, P. citrinopileatus and P. salmoneostramineus is low.
Characteristics of microorganism isolated from Cotton Waste Media for the Oyster Mushroom Cultivation
Lee, Chan-Jung ; Jhune, Chang-Sung ; Cheong, Jong-Chun ; Oh, Jin-A ; Han, Hye-Su ; Um, Na-Na ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 120~124
DOI : 10.4489/KJM.2010.38.2.120
This study was carried out to investigate interaction between mushroom mycelium and microorganisms in cotton waste media for the shelf cultivation of oyster mushroom. Two oyster mushroom farms was selected for this experiment. One was good mushroom farm (farmhouse I) and the other failed mushroom farm (farmhouse II). In farmhouse I, the inhibition microorganisms were higher toward the end of growth stage than the early stage, but the result of farmhouse II was opposite. Effects of the mycelium growth on plate culture showed same results on mushrooms as the earlier one. And the mycelium growth was influenced by secretory materials of microorganisms. Among of the isolates, Only few microorganism had inhibitory effects on either P. tolaasii or T. harzianum causing the disease of oyster mushrooms. But more microorganisms had inhibition effects on P. agarici.
Cultural Characteristics by Sawdust and Liquid Spawn for the Cultivation of Neolentinus lepideus
Jang, Myoung-Jun ; Lee, Yun-Hae ; Ju, Young-Cheol ; Koo, Han-Mo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 125~129
DOI : 10.4489/KJM.2010.38.2.125
This study was carried out to elucidate suitable spawn culture for an artificial cultivation of Neolentinus lepideus. The optimum culture conditions of the liquid spawn were deffated soy flour for main material, 12 days for culture period and 0.9 vvm for aeration volume, respectively. Sawdust spawn was appropriate for douglas fir sawdust and deffated corn flour (95 : 5, v/v) for mycelia growth and fruiting body formation. In case of liquid spawn, cultivation period was two shorter than sawdust spawn and mushroom yield was 111.9 g per 850ml cultivation bottle. In conclusion, the suitable spawn for fruiting body production was found to be liquid spawn than sawdust spawn with considering cultivation period and mushroom productivity.
Development of New Mushroom Substrate using Kapok Seedcake for Bottle Culture of Oyster Mushroom(Pleurotus ostreatus).
Won, Seon-Yi ; Lee, Yun-Hae ; Jeon, Dae-Hoon ; Ju, Young-Cheoul ; Lee, Yong-Beom ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 130~135
DOI : 10.4489/KJM.2010.38.2.130
To select the viable alternative substrates among the variable organic substrates for productivity enhancement and production cost-reduction of oyster mushroom in bottle culture, this study was carried out at mushroom research institute of GGRDA in 2007. In bottle culture of oyster mushroom (Plerutus ostreatus), the seedcakes of rape (RS), soybean (SS), coconut (CCS), and kapok (KS) were examined as substitute of cotton seedcake which was primary nutritive material of mushroom growing substrate. The chemical properties of substrate mixed with kapok seedcake is similar to the mixture with cotton seedcake in T-C, T-N, C/N ratio, and other nutrients. Mixed growing substrate containing cotton seedcake and kapok seedcake was superior to other mixtures 99.2% and 99.5%, respectively in spawning ratio and was faster mycellium growth in column test than that of soybeen seedcake, cotton + soybeen seedcake, and coconut seedcake. The period required in first pin-heading was 1-2 days longer in rape and soybeen seedcake mixture. Also there wad no primodia and fruitbody formation at soybeen seedcake mixture which had highest T-N content among the other mixed substrates. Yield per bottle and biological efficiency were highest of 144.6 g and 75.4%, respectively at kapok seedcake mixture. As a result, this study found that cotton seedcake can be replaced with kapok seedcake in bottle culture of oyster mushroom.
Physico-chemical Characteristics and Utilization of Raw Materials for Mushroom Substrates
Cheong, Jong-Chun ; Jhune, Chang-Sung ; Lee, Chan-Jung ; Oh, Jin-A ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 136~141
DOI : 10.4489/KJM.2010.38.2.136
To provide a basic information on the chemical concentrations of different raw materials used in mushroom cultivation, the raw materials were collected from 13 Flammulina velutipes, 14 Pleurotus eryngii, and 8 P. ostreatus farms and analyzed to calculate moisture contents, pH, total carbon(T-C), total nitrate(T-N), total phosphate(T-P) and 13 different cations. In our results, the C : N ratios of cotton seed meal, beet pulp, and corn-cob were 6~17, 12~29 and 56~127, respectively. Depending on the companies which process these materials, the range of C:N ratio of soybean curd residue was approximately either 8~9 or 14~17 with wider range of C : N ratio of the raw materials imported from other countries without the detection of heavy metals. In this study, the formula was provided to calculate the composition of mixed media for mushroom cultivation based on the ingredient chart of different raw materials.
Selection of an Substitute Sawdust Material in Pleurotus ostreatus by Bottle Cultivation
Jang, Myoung-Jun ; Lee, Yun-Hae ; Ju, Young-Cheol ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 142~145
DOI : 10.4489/KJM.2010.38.2.142
In this study, we attempted to find substitute materials, swelling rice hull, cocopeat, corncob and coconut sawdust, for sawdust in bottle cultivation of Pleurotus ostreatus. Chemical characters of mixture substrates with four substitute materials for pine sawdust were not different significantly. By comparison in mycelial growth and yield of fruitbody, mixture substrate of cocopeat were showed the same level in P. ostreatus. Therefore, it is suggested that cocopeat was substituted for sawdust for cultivation of P. ostreatus.
Isolation Frequency of Candida species from Clinical Specimens
Shin, Hyun-Sung ; Park, Youn-Bo ; Shin, Du-Sik ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 146~151
DOI : 10.4489/KJM.2010.38.2.146
A total of 167,955 microorganisms were isolated from 366,661 clinical specimens. Among them, 6,517 strains of the Candida spp. were isolated from the department of laboratory medicine in "C" hospital from Jan. 1, 2005 to Dec. 31, 2009. All clinical specimens were reviewed by the medical records of patients with Candida by the method of retrospectiveness. From this, we got the some isolated pure cultured yeasts. We identified these yeast by the identification kit system of VITEKII and VITEKII-ID-YST card. The isolation frequencies of Candida spp. were as follows. 56.4%,of C. albicans, 17.7% C tropicalis, 10.7% C glabrata and 9.5% C parapsilosis. The isolated frequency of Candida spp. in 2009 was 1.9 times higher than that in 2005. The clinical materials showing over 10.0% isolation rate were in sputum (30.1%), random urine (25.0%), 15.8% blood (15.8%) and catheterized urine (13.5%) in Candida spp.. The clinical department of showing over 7.0% isolation rate were in pulmonary medicine (20.5%), renal medicine (11.0%), infection disease medicine (10.4%), critical care medicine (10.0%), hematooncology (9.6%), general surgery (7.5%) and gastrointestinal medicine (7.4%) in Candida spp.. In monthly analysis, Candida spp. were most friquency isolated in July (10.6%), but lowest one in February (6.1%). Candida spp. were most frequently isolated in patient of over 50 years old (16.7-40.1%) than those isolated from the patients under the age of 0-49 (1.3-7.5%).
Purification and Characterization of Urushiol Induced Laccase Isoenzyme from Fomitella fraxinea
Choi, Han-Seok ; Park, Hyo-Suk ; Yeo, Soo-Hwan ; Jeong, Seok-Tae ; Choi, Ji-Ho ; Kim, Myung-Kon ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 152~159
DOI : 10.4489/KJM.2010.38.2.152
The influence of urushiol, as an allergen on laccase property of Fomitella fraxinea was investigated. The enzyme production was reached to the highest level after 10 days, cultivation and the activity and mycelial biomass were increased by 2.5 and 1.5 folds, respectively, by adding urushiol in the culture medium. In liquid cultures using a Cu Mn-free medium, laccase lactivity was decreased by 3.8-9.2 folds, with similar dry cell weight. Two isoenzymes, were purified using anion exchange, hydrophobic interaction and size-exclusion chromatographies. Both isoenzymes are monomeric proteins, with
around 67 kDa(Lac1) and 66 kDa(Lac2), and isoelectric points of 3.67 and 3.81. The optimal conditions for purified isoenzymes were found to be pH 4.5-5.0 and
. Activity decreased by the addition of
, and strongly inhibited by EDTA and sodium azide.
Gene Structure and Function of fkhE, a Forkhead Gene in a Filamentous Fungus Aspergillus nidulans
Park, Mi-Hye ; Kim, Hyoun-Young ; Kim, Jong-Hwa ; Han, Kap-Hoon ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 160~166
DOI : 10.4489/KJM.2010.38.2.160
A homothallic filamentous fungus Aspergillus nidulans has been used as the a model organism for studying growth and development for eukaryotic system. Various studies about specific transcription factors have been performed for elucidating the molecular mechanisms of growth, asexual and sexual developmental processes. Among them, the fkhE gene (AN2025.3) is located in chromosome VII and contains an ORF encoding 718 amino acid polypeptide intervening with two short introns. The cDNA sequencing revealed that at least four types of alternative splicing events were occurred when the fkhE gene was transcribed. The putative FkhE polypeptide contains a conserved forkhead domain and a bipartite nuclear localization signal at it's N-terminus and C-terminus, respectively. Deletion of fkhE resulted in impaired conidiophore formation in a solid medium. However, the sexual developmental process or cleistothecia formation was normal. Furthermore, fkhE deletion mutant produced conidiophores and conidia under the submerged culture, indicating that the fkhE gene is involved in asexual developmental process similar to the fkhF gene.
Inhibitory Effects of Melanin Secretion on B16 Melanoma cell of Cordyceps militaris Water Extract
Nam, Byung-Hyouk ; Jo, Wool-Soon ; Choi, Yoo-Jin ; Lee, Jae-Yun ; Kang, Eun-Young ; Jeong, Min-Ho ; Lee, Jae-Dong ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 167~171
DOI : 10.4489/KJM.2010.38.2.167
The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at
, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (> 50%) melanin synthesis at
. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.
Physicochemical Properties and Physiological Activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea
Choi, Han-Seok ; Kim, Bo-Hyun ; Yeo, Soo-Hwan ; Jeong, Seok-Tae ; Choi, Ji-Ho ; Park, Hyo-Suk ; Kim, Myung-Kon ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 172~178
DOI : 10.4489/KJM.2010.38.2.172
The contents of proximate composition, free amino acids and phenolic acids in the Fomitella fraxinea cultivated-Rhus verniciflua stem bark(FRVSB), and its adipogenesis effect were investigated. The proximate composition(%) of FRVSB was as follows: moisture(7.64), ash(6.30), crude fat(3.86), crude protein(3.59) and sugar(not detected); while Rhus verniciflua stem bark(RVSB) contained 1.64, 8.09, 7.28, 6.48 and 5.39, respectively. The total free amino acids concentration was 97.41 mg% in FRVSB and 71.91 mg% in RVSB. Phosphoserine(55.06 mg%), ammonia(17.84mg%) and aspartic acid(6.05mg%) were predominant amino acids. The content of total phenolic acids was 422.89 ppm in ethanol extract and 283.86 ppm in water extract, with syringic and gallic acid as the main component. The FRVSB extracts showed a potent free radical scavenging activity for DPPH(2,2-diphenyl-1-picrylhydrazyl hydrate) with
(water), respectively, whereas
value of gallic acid was
. The protective effect of both ethanol and water extract the extracts against UV-induced oxidative stress in NIH3T3 was observed. The water extracts of FRVSB may promote adipogenesis in 3T3-L1 cells.
Characterization of Anti-inflammation Effect of Aqueous Extracts from Phellinus baumii
Kim, Hye-Min ; Lee, Dong-Hee ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 179~183
DOI : 10.4489/KJM.2010.38.2.179
This study is to characterize the postulated anti-inflammatory effect of the hot water extracts from the Phellinus baumii. RAW264.7, macrophage cell line, was activated by lipopolysaccharide (LPS) and, further, treated with Phellinus baumii's aqueous extract. When the cultured macrophage cells were treated with LPS, they show typical signs of endoplasmic reticulum stress (ERS) and an increment in secretion of inflammatory cytokine compared to the non-treated control: The expression of glucose-regulated protein78 (Grp78), Grp94, and C/EBP homologous protein/GADD 153 (CHOP) increased along with augmented secretion of interlukin-6. Cellular nitric oxide content also significantly went up in comparison to the non-LPS treatment. When the LPS-treated RAW264.7 was treated with the aqueous Phellinus baumii extracts, however, the expression of ERS markers markedly reduced and the release of nitric oxide declined. Also, the expression of induced nitric oxide synthase (iNOS) notably diminished similarly as the NO content. In conclusion, this study strongly indicated that aqueous Phellinus baumii extract can be utilized directly as anti-inflammation agent and serves as a source of functional ingredient to lessen the inflammation.
Screening of Yeast for Brewing of Korean Traditional Pear Yakju and Optimal Fermentation Condition
Song, Jung-Hwa ; Jang, Jeong-Hoon ; Na, Kwang-Chul ; Kim, Ha-Kun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 184~188
DOI : 10.4489/KJM.2010.38.2.184
The goal of this study was to screen a useful yeast for Korean traditional pear Yakju (KTPY) brewing and develop its brewing process. Cooked non-glutinous rice and nuruk were mixed, and added into pear juice with various Saccharomyces cerevisiae and then fermented at
for 7 days. Among several alcohol fermentation yeasts, ethanol contents was the highest in pear Yakju made by S. cerevisiae K-7 and also showed high ethanol content in pear Yakju which was made by commercial S. cerevisiae C-2. Therefore, we selected S. cerevisiae K-7 and S. cerevisiae C-2 as suitable yeasts for brewing of KTPY. Maximal ethanol production (10.4%) was obtained when cooked non-glutinous rice (100 g) and nuruk (30 sp/g) were mixed and added into pear juice (600 ml) with S. cerevisiae K-7 (5%) and fermented at
for 7 days and also its antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activity was 57.2%. Addition of antihypertensive starchy materials into the mash was not affected in ACE inhibitory activity and total acceptability of KTPY.
Manufacture and Characteristics of Functional Drink Using Pear-Strawberry Fermentative Concentrates from Fermentation by Saccharomyces cerevisiae C-2
Jang, Jeong-Hoon ; Na, Kwang-Chul ; Kim, Wal-Soo ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 189~191
DOI : 10.4489/KJM.2010.38.2.189
Mixed juice of pear and strawberry was fermented by commercial Saccharomyces cerevisiae C-2 at
for 7 days and obtained the pear-strawberry fermentative concentrates (PSFC). The PSFC showed high ACE inhibitory activity of 70.8%. The PSFC drink was prepared by using PSFC and by-materials including amylopeptide, and determined changes of quality and ACE inhibitory activity during storage of
. PSFC drink was very stable at storage of
for 8 weeks without any quality and ACE inhibitory activity.
Some Unrecorded Genera and Species in Korean Agaricales (I)
Seok, Soon-Ja ; Kim, Yang-Sup ; Suh, Jang-Sun ; Kwon, Soon-Woo ; Park, In-Cheol ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 192~196
DOI : 10.4489/KJM.2010.38.2.192
Two species of the Korean Agaricales are described and illustrated in this paper. Two genera Cystoagaricus and Limacella are new to Korean agaric flora. All of these, Cystoagaricus strobilomyces and Limacella glioderma are recorded for the first time in the Korean fungus flora. The collections cited are all preserved in the Herbarium Conservation Center of National Academy of Agricultural Sciences.
Note on the New Record of Russula eburneoareolata Hongo in Korea
Shin, Kwang-Soo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 197~198
DOI : 10.4489/KJM.2010.38.2.197
The morphological and ecological characteristics of Russula eburneoareolata Hongo is reported first time in Korea. The fungus was collected from the mixed forest of Fagaceae in the Gyeryongsan Mountain National Park. The pileus is champagne to ivory, convex then expanded and depressed in the center afterward, and 4 to 9 cm broad. Stipe is 3 to 6 cm long, 12 to 20mm thick and tapering below. Spores are
, subspherical oval and warty with some connecting lines. Basidia are about
and four-spore bearing type.
Fruit-body Production of Ganoderma neo-japonicum by Sawdust Cultivation
Jo, Woo-Sik ; Park, Ha-Na ; Park, Shin-Hye ; Jung, Hee-Young ; Yoo, Young-Bok ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 199~201
DOI : 10.4489/KJM.2010.38.2.199
Ganoderma neo-japonicum, which is also known as black lingshi mushroom and medicinal mushroom. Present experiments were conducted to determine the possibility of artificial culture with oak sawdust of G. neo-japonicum. The duration of mycelial growth and days of pinhead formation of oak sawdust bag (2.4 kg) were 28~35 days and 25~29 days, respectively. The yield of mushroom fresh fruitbody was 135~157 g.
Tyrosinase Inhibitory Activity of Macrolepiota procera
Kwak, Jung-Hoon ; Han, Yeong-Hwan ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 202~204
DOI : 10.4489/KJM.2010.38.2.202
Inhibitory effect on tyrosinase activity and melanogenesis of the mycelia and mycelial culture broth of Macrolepiota procera were investigated. The methanol fraction of culture broth showed 56.6% of tyrosinase inhibitory activity and its IC50 was 8.78 mg/ml. Using Streptococcus bikiniensis for melanogenesis in vitro, the methanol extract of mycelia showed 94.0% inhibition of melanogenesis.
Occurrence of Stem Rot of Valeriana fauriei Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Jeong, Seon-Gi ; Jin, Young-Min ; Song, Won-Doo ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 205~207
DOI : 10.4489/KJM.2010.38.2.205
Stem and petiole rot symptoms of Valeriana fauriei occurred sporadically in the herb exhibition field in Gyeongsangnam-do Agricultural Research and Extension Services, Hamyang-gun, Gyeongnam Province in Korea. The typical symptom is water-soaking on the stem, rotting, wilting, blighting and the infected plants eventually died. White mycelial mats spreaded over lesions, and then sclerotia were formed on the infected plant parts and near soil surface line. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii. This is the first report of stem rot on Valeriana fauriei caused by Sclerotium rolfsii in Korea.
Occurrence of Downy Mildew on Rape in Korea and Resistance of Rape Varieties to the Disease
Kim, Wan-Gyu ; Hong, Sung-Kee ; Choi, Hyo-Won ; Myung, Inn-Shik ; Choi, In-Hu ;
The Korean Journal of Mycology, volume 38, issue 2, 2010, Pages 208~209
DOI : 10.4489/KJM.2010.38.2.208
Downy mildew commonly occurred in six of seven locations investigated in Korea during disease survey performed in 2007 and 2008. This report provides screened results on resistance of Korean varieties of rape to downy mildew in the field.