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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 39, Issue 3 - Dec 2011
Volume 39, Issue 2 - Aug 2011
Volume 39, Issue 1 - Apr 2011
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The Optimal Culture Conditions and Antifungal Activity of Culture Extract from Oudemansiella mucida
Choi, Mi-Ryue ; Cho, Hae-Jin ; Lee, Jae-Seong ; Kim, Hye-Young ; Lee, Tae-Soo ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 91~98
DOI : 10.4489/KJM.2010.39.2.091
Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were
and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants.
Assessment of the Ability of Extracellular Enzyme Production in Hybrid Strains of Lentinula edodes by Chromogenic Reaction-based Plate Assay
Kwon, Hyuk-Woo ; Kim, Jun-Young ; Ko, Han-Gyu ; Park, Heung-Soo ; Kim, Seong-Hwan ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 99~104
DOI : 10.4489/KJM.2010.39.2.099
Shiitake breeding requires the procedures of mating of two different parental strains and selection of hybrid strains that have good traits for the mushroom production. In this study, we tested the possibility of the use of chromogenic plate-based assay for extracellular enzyme production in order to assess and find good biochemical properties-possessed hybrid strains that were generated from genetic cross of the monokaryotic strains derived from two different parental strains of Lentinula edodes Sanjo-101ho and Sanjo-108ho. We observed that there was difference in the ability of producing
-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease among the monokaryotic strains. We could also comparatively assess that the ability of the seven extracellular enzymes production in the hybrid strains depended on the mating combination of the monokaryotic strains. Our results demonstrate that the assessment method for extracellular enzyme production using chromogenic plate assay could be usefully applied to the assessment of the hybrid strains derived from the breeding procedure of L. edodes.
Physiological Activities of Extracts from Fomitella fraxinea on Brown Rice added Rice Bran, Pine Needle and Tumeric Powder
Park, Hyo-Suk ; Jeon, Tae-Woog ; Choi, Han-Seok ; Kim, Joong-Man ; Kim, Myung-Kon ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 105~110
DOI : 10.4489/KJM.2010.39.2.105
This study was carried out to examine the electron donating ability (EDA), nitrite scavenging, tyrosinase inhibition, ACE inhibition and fibrinolytic activity of culture extracts from Fomitella fraxinea which was grown added rice bran, pine needles and turmeric in brown rice. Electron donating ability was higher in the extract by hot water than ethanol. Nitrite scavenging activity was the highest from ethanol extract of culture products added to the pine needles, showed the scavenging activity was approximately 50%. Tyrosinase inhibition activity was highest in the extract by hot water, showed inhibition rate of rice bran was 72.49%, pine needles 87.34% and turmeric powder 82.75%, respectively. ACE inhibition activity were very low effective at all water and ethanol extracts. Fibrinolytic activities were similarly strong in the rice bran, pine needles and turmeric powder. Especially, when the rice bran and turmeric powder were added, showed the activities were increased about 10% than plasmin. Therefore, it may be used for the food and cosmetic industry as natural source of bioactive compound after further investigation such as in vivo experiment.
Physiological Activities of Extracts from Phellinus linteus on Brown Rice added Rice Bran, Pine Needle and Tumeric Powder
Park, Hyo-Suk ; Jeon, Tae-Woog ; Choi, Han-Seok ; Kim, Joong-Man ; Kim, Myung-Kon ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 111~116
DOI : 10.4489/KJM.2010.39.2.111
This study was carried out to examine Electron donating ability (EDA), nitrite scavenging, tyrosinase inhibition, ACE inhibition activity and fibrinolytic activity of culture extracts from Phellinus linteus which was grown added rice bran, pine needles and turmeric in brown rice. Electron donating ability of Phellinus linteus extract (PLE) was lower in the water extract than the ethanol extract. Nitrite scavenging activity was the highest in PLE from ethanol extract than water extract. Especially, when the pine needles was addition treatment, the nitrite scavenging activity was about 70% at pH 1.2 by ethanol extract. Tyrosinase inhibition activity of PLE was highest in the water extract than ethanol extract, and inhibition rate was the most higher in the extract by hot water added pine needles. ACE inhibition activity were very low effective at water and ethanol extract. Fibrinolytic activities were similarly strong in rice bran, pine needles and turmeric powder. Especially, when rice bran was added, showed the activity was increased about 5% than plasmin. Therefore, It may be used for the food industry as natural source of bioactive compound after further investigation, such as in vivo experiment.
Optimization of Anticoagulant Production from Phellinus linteus Mycelia
Seo, Ho-Chan ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 117~121
DOI : 10.4489/KJM.2010.39.2.117
To produce the functional food materials, 50 kinds of the mycelial extracts from edible mushroom were examined for anticoagulant activity and Phellinus linteus showed the highest activity through the activated partial thromboplastin test (aPTT). The maximum production of anticoagulant activity and the mycelial growth was observed in culture medium containing soluble starch 3.0%, peptone 0.1%,
0.1% and in the culture conditions controlled at initial pH 7.0,
and 150 rpm by the rotary shaker. In addition, the maximum production of mycelial dry weight was 7.5 mg/mL after 10 days under the optimal conditions, and anticoagulant activity was reached to 390 sec in 5 L-jar fermentor.
Changes of Quality Characteristics during Storage of Gugija-Liriope tuber Makgeolli Made by Saccharomyces cerevisiae C-2
Baek, Seung-Ye ; Nam, Yun-Gyu ; Ju, Jung-Il ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 122~125
DOI : 10.4489/KJM.2010.39.2.122
The goal of this study was to provide shelf-life of Gugija-Liriope tuber raw makgeolli. After we prepared Gugija-Liriope tuber raw makgeolli by using Gugija, Liriope tuber, nuruk, cooked rice and Saccharomyces cerevisiae C-2, changes of its quality and physiological functionality were investigated during the storage at
. Ethanol contents was decreased from 8.3% to 7.2% for storage of 9 days at
and 6 days storage at
, respectively. Antihypertensive angiotensin I-converting enzyme inhibitory activity of Gugija-Liriope tuber makgeolli was increased from 63.1% to 66.0% after storage for 9 days at
. Total acceptability of 9 days storage makgeolli at
was still good. In conclusion, shelf-life of Gugija-Liriope tuber raw makgeolli was 9 days at
in this respect of ethanol contents, antihypertensive action and total acceptability.
Screening of Multifunctional Bacteria with Biocontrol and Biofertilizing Effects
Kim, Young-Sook ; Lee, Myeong-Seok ; Yeom, Ji-Hee ; Song, Ja-Gyeong ; Lee, In-Kyoung ; Yun, Bong-Sik ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 126~130
DOI : 10.4489/KJM.2010.39.2.126
In the course of search for multifunctional microbial inoculants, three Bacillus strains (BS11-1,BS11-2,BS11-3) with biological control and biofertilizing effects were selected. In this study, their ability for solubilization of insoluble phosphate, production of indole-3-acetic acid (IAA), siderophore, and hydrolytic enzymes, and antagonism against phytopathogenic fungi were estimated. All strains produced IAA and siderophore depending on culture time and produced a visible clear zone on agar plate containing 0.5% carboxylmethyl cellulose as a carbon source. Also, these strains exhibited antifungal activities against phytopathogenic fungi, Botrytis cinerea, Cylindrocarpon destructans, Fusarium oxysporum, Rhizoctonia solani, and Phytophthora capsici.
Inhibitory Effect of Paraconiothyrium minitans CM2 on Sclerotial Germination of Sclerotinia sclerotiorum and S. minor Causing Sclerotinia Rot of Lettuce
Lee, Sang-Yeob ; Kim, Wan-Gyu ; Hong, Sung-Kee ; Weon, Hang-Yeon ; Park, Kyung-Seok ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 131~135
DOI : 10.4489/KJM.2010.39.2.131
One fungal isolate CM2 parasitic to Sclerotinia sclerotiorum and S. minor causing Sclerotinia rot of lettuce was identified as Paraconiothyrium minitans based on its morphological and molecular characteristics. P. minitans CM2 grew best on PDA with pH 6.5 at
under alternating cycles of 12 hr near ultraviolet light and 12 hr darkness. Scleroria of S. sclerotiorum and S. minor treated with conidial suspension of P. minitans CM2 did not directly germinate and produced no apothecia.
Note on Some Species of the Laboulbeniales Newly Collected from Korea(2011)
Lee, Yong-Bo ; Lim, Chae-Kyu ; Na, Young-Hee ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 136~140
DOI : 10.4489/KJM.2010.39.2.136
Five species of the Laboulbeniales are reported to the Korean mycoflora. They are Laboulbenia acupalpi Spegazzini on Acupalpus hilaris Tschitscherine, L. pseudomasei Thaxter on Anoplogenius cyanescens(Hope), Rickia peyerimhoffii Maire on Scaphidium femorale Lewis, Zodiomyces odae Majewski et Sugiyama on Sternolophus rufipes Fabr. and Z. vorticellarius Thaxter on Berosus fairmairei Zaitzev. Among these species L. pseudomasei Thaxter and Z. odae Majewski et Sugiyama are the first report to South Korea. L. acupalpi Spegazzini and Z. vorticellarius Thaxter found on species which are different from previous to host insects. R. peyerimhoffii Maire is newly described main land of Korea, although it was reported from Jeju island.
Comparison of Viability in Basidiomycetes After Low Temperature Storage According to Storage Period
Ryu, Sung-Ryul ; Ka, Kang-Hyeon ; Lee, Bong-Hun ; Park, Hyun ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 141~144
DOI : 10.4489/KJM.2010.39.2.141
Short-preservation of basidiomycetes is generally being conducted in slant tubes containing solid medium based on agar. In this study, we investigated the vitality of 28 species and 76 strains preserved on potato dextrose agar (PDA) at
for 2~7 years. The survival rates of the fungi were 82%, 86%, 94%, 96%, 94%, and 94% for seven, six, five, four, three, and two years old preservation, respectively. The volume of medium in Lentinula edodes showed decrease after 2 years preserved. The pH of preserved medium was 5.42 in 2007 (two years old), but it became nearly neutral as increasing preservation term.
Stem Rot of Convallaria keiskei Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Lee, Heung-Soo ; Kang, Dong-Wan ; Kwack, Yong-Bum ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 145~147
DOI : 10.4489/KJM.2010.39.2.145
Stem rot symptoms of Convallaria keiskei occurred sporadically in the herb exhibition field at Hamyang-gun Gyeongnam province in Korea. The typical symptom is water-soaking on the main stem, rotting, wilting, and blighting, which eventually leads to death of the plant. The sclerotia, which were white to brown in color, globoid in shape with size of 1~3 mm hyphal width of which was 4~9
, were formed over lesions and surface soil line. The optimum temperature for mycelial growth and sclerotia formation was
on PDA. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. Mycological characteristics and pathogenicity to host plants indicated that the infected fungus was Sclerotium rolfsii Saccardo. This is the first report of stem rot on Convallaria keiskei caused by S. rolfsii in Korea.
Stimulatory Effect of Cordyceps militaris on Testosterone Production in Male Mouse
Hong, In-Pyo ; Choi, Yong-Soo ; Woo, Soon-Ok ; Han, Sang-Mi ; Kim, Hye-Kyung ; Lee, Myung-Ryul ; Nam, Sung-Hee ; Ha, Nam-Gyu ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 148~150
DOI : 10.4489/KJM.2010.39.2.148
Cordyceps militaris(CM) has been used as a traditional folk medicine to strengthen the immune system and regain energy for hundreds of years in Far East Asian countries. In the present studies, we determined that the stimulatory effect of Cordyceps militaris on testosterone production in male mouse rats. Results illustrated that changes of the body weight, food and water intake of the rats were not observed in this study but the concentration of testosterone in the serum of the rats was significantly increased by CM(p < 0.05). Therefore fruiting bodies of CM grown on the drone bee medium may be an integrative medicine for the treatment of reproductive problems caused by insufficient testosterone levels in human males.
Changes of Yeasts and Bacterial Flora during the Storage of Korean Traditional Makgeolli
Min, Jin-Hong ; Baek, Seung-Ye ; Lee, Jong-Soo ; Kim, Ha-Kun ;
The Korean Journal of Mycology, volume 39, issue 2, 2011, Pages 151~153
DOI : 10.4489/KJM.2010.39.2.151
In order to optimize storage conditions of Korean traditional Makgeolli, we brewed Korean traditional Makgeolli for 1 week with two-stage fermentations and investigated changes viable cell counts of yeasts and bacteria during storage for 1 month at
. Yeast viable cell counts were decreased to 89.0% after storage for 30 days at
, however, those were not significantly changed at
storage. Bacteria cell counts were decreased to 59.0% of initial cell counts for 30 days at
. In the storage at
, bacteria were significantly decreased to 98.0% of initial cell counts after storage for 30 days. Lactic acid bacteria were also similar to those of total bacteria cell counts at
storage, however, 99% of lactic acid bacteria were decreased at
storage for 30 days.