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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 40, Issue 4 - Dec 2012
Volume 40, Issue 3 - Sep 2012
Volume 40, Issue 2 - Jun 2012
Volume 40, Issue 1 - Apr 2012
Selecting the target year
Diversities of Arbuscular Mycorrhizal Fungi in Cultivated Field Soils of Korean Ginseng
Kil, Yi-Jong ; Eo, Ju-Kyeong ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 1~6
DOI : 10.4489/KJM.2012.40.1.001
In this study, soil samples were collected from cultivated fields of 1-5 year old Korean ginseng in Geumsan, Korea. Spores of arbuscular mycorrhizal fungi were extracted from soils and identified using morphological characteristics and 18s rDNA sequences of the spores. Total 10 species of AMF were identified: Acaulospora longula, Archaeospora trappei, Glomus caledonium, Glomus etunicatum, Glomus intraradices, Glomus mosseae, Glomus sp., Paraglomus occultum, Paraglomus brasilianum, and Scutellospora heterogama. Relative abundance of spores of A. trappei were increased with increase of cultivation period of the ginseng. However, relative abundance of other species of AMF and Shannon diversity (H`) of AMF were significantly decreased with the increase of cultivation periods of the ginseng.
Genetic Diversity of Endophytic Fungi Isolated from the Roots of Halophytes Naturally Growing in Suncheon Bay
Seo, Yeong-Gyo ; Kim, Mi-Ae ; You, Young-Hyun ; Yoon, Hyeok-Jun ; Woo, Ju-Ri ; Lee, Gyeong-Min ; Kim, Jong-Guk ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 7~10
DOI : 10.4489/KJM.2012.40.1.007
Endophytic fungi were isolated from the roots of halophytes, Suaeda japonica and Carex scabrifolia in the Suncheonbay. The ITS region in rDNA of 15 endophytic fungal strains were amplified using PCR with universal primers ITS1 and ITS4, and those amplified fragments were sequenced. Based on ITS sequence, five fungal genera were identified in S. japonica and seven fungal genera were identified in C. scabrifolia. The Shannon`s diversity index (H`) of endophytic fungi isolated from S. japonica and C. scabrifolia was 1.561 and 1.889, respectively. In phylogenetic analysis, it was shown that Ascomycota and Pezizomycotina was widely distributed both in S. japonica and C. scabrifolia. Also, Sordariomycetes, Dothideomycetes and Eurotiomycetes were shown to be distributed in these halophytes used in this experiment.
Genetic Diversity of Penicillium isolates Isolated from Pears with Postharvest Decay in Storage
Han, Do-Suk ; Hong, Sung-Kee ; Kang, Hee-Wan ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 11~18
DOI : 10.4489/KJM.2012.40.1.011
This study was carried out to identify the genetic diversity of Penicillium isolates that were isolated from pears with postharvest decay in storage. URP-PCR was used to detect DNA diversity of 84 Penicillium isolates. Based on URP-PCR profiles, 18 Penicillium isolates were selected and their PCR polymorphic bands were produced by additional primers URP1F, URP2R, URP2F, and URP4R. UPGMA cluster analysis using the polymorphic bands showed four clustered groups and futhermore cultural and morphological features characterized the 18 Penicillium isolates. Group 1 was dominant, which occupies 70% in the four clustered groups and identified as P. expansum based on ITS sequence and morphological features.
Relationship between Higher Fungi Distribution and Climatic Factors in Naejangsan National Park
Jang, Seog-Ki ; Kim, Sang-Wook ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 19~38
DOI : 10.4489/KJM.2012.40.1.019
This study was conducted to investigate the diversity of higher fungi and relationship between higher fungi and climatic factors in Naejangsan National Park from April 2004 to October 2010. The obtained results from investigation were as follows. The higher fungi were classified into 48 families, 158 genera and 451 species in Basidiomycotina, 13 families, 26 genera and 39 species in Ascomycotina, and 4 families, 7 genera and 7 species in Myxomycetes, and most of them belonged to Hymenomycetidae in Basidiomycotina. Dominant species belonged to Ttricholomataceae (72 species), Russulaceae (39 species), Polyporaceae (41 species), Boletaceae (40 species), Cortinariaceae (35 species) and Amamtaceae (28 species). For the habitat environment, the ectomycorrhizal mushrooms were 38.8% (15 families, 36 genera and 193 species), litter decomposing and wood rotting fungi 39.4% (36 families, 107 genera and 196 species), grounding Fungi 19.9% (24 families, 51 genera and 99 species) and others 1.8% (3 families, 4 genera and 9 species). Monthly, most of higher fungi were found in July, August and September, and least found in November. In climatic conditions, most higher fungi were occurred in
and above of mean temperature,
and above of minimum temperature, and
and above of maximum temperature. most of higher fungi were found in 73% and above of relative humidity and 200 mm and above of monthly precipitation. In case of ectomycorrhizal fungi like Amamtaceae, Boletaceae and Cortinariaceae, significance levels are not high in
and above of maximum temperature which mostly affects species occurrence than other climatic factors of mean and minimum temperature and monthly precipitation.
Species and Distribution of Native Wild Mushrooms Traditionally using in Jeju Island
Ko, Pyung-Yeol ; Lee, Seung-Hak ; Jeun, Yong-Chull ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 39~43
DOI : 10.4489/KJM.2012.40.1.039
The knowledge of native wild mushrooms traditionally using in Jeju Island, that have specific native environment and various biodiversity, was investigated. From Jeju and Seogwipo cities including 7 provinces 4 locals, so total 50 villages, data was collected in the entire area and voice of the 93 elderly people between the age of fifty and ninety were recorded. As a result, total 23 native wild mushrooms were used in which 2 species were poisonous mushrooms, 7 species were medicinal mushrooms, 2 species was used for folk religion, 2 species were not used for living but for attention and 12 species were edible mushrooms. Also, a total of 267 cases of traditional knowledge was collected, in which 197 cases were about edible mushrooms for 12 species, 43 cases were about poisonous mushrooms for 2 species, 16 cases were about medicinal mushrooms for 7 species. Interestingly, the fortune for agriculture was told depending on spore mass release of Cyathus stercoreus which grows in animal feces and compost. It is considered as the distinctive traditional knowledge of Jeju Island.
Screening of Antagonistic Bacteria for Biological control of Ginseng Root Rot
Kim, Young-Sook ; Lee, Myeong-Seok ; Yeom, Ji-Hee ; Song, Ja-Gyeong ; Lee, In-Kyoung ; Yeo, Woon-Hyung ; Yun, Bong-Sik ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 44~48
DOI : 10.4489/KJM.2012.40.1.044
Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herb in Korea. However, yield losses reached up to 30~60 % due to various diseases during 3 or 5 years of ginseng cultivation. Therefore, successful production of ginseng roots depends primarily on the control of diseases. The objective of this study is to select potential multifunctional biocontrol agents from actinomycetes for the control of multiple ginseng diseases as an alternative to fungicides. Ninety three Streptomyces strains were selected and their ability to produce antibiotics, siderophore and lytic enzymes such as protease and cellulose were investigated. Eight of the isolates, strains A75, A501, 515, 523, A704, A1444, A3265 and A3283 produced cellulase and protease. These strains also produced siderophore and showed potent antifungal activity against Botrytis cinerea, Cylindrocarpon destructans, Collectotricum gloeosporioides, Phytophthora capsici and Rhizoctonia solani causing ginseng root rot.
Development of the Optimal Media for Mycelial Culture of Pleurotus eryngii using the Hot-water Extract of Raw Materials
Kim, Min-Keun ; Ryu, Jae-San ; Lee, Young-Han ; Lee, Seong-Tae ; Heo, Jae-Young ; Kwon, Jin-Hyeuk ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 49~53
DOI : 10.4489/KJM.2012.40.1.049
Hot-water extracted natural media were made from raw materials for mycelial culture of Pleurotus eryngii. Poplar sawdust, wheat bran and rice bran were used as substrates for hot water extraction. The mixed substrates of poplar sawdust, wheat bran, and rice bran with 50 : 20 : 30 (v/v/v, PWR523) and 50 : 30 : 20 (v/v/v, PWR532) were optimal for mycelial growth of P. eryngii, respectively. The hot-water extracted natural media from PWR523 and PWR532 showed a rapid mycelial growth and spawn running compared to PDA. There was no significant difference in mushroom yield when the mycelium grown on the hot-water extracted natural media was used as the inoculum source for producing fruit body.
Effect of Beta-glucans Extracted from Phellinus baumii on the Growth of Caenorhabditis elegans
Kim, Hye-Min ; Lee, Dong-Hee ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 54~59
DOI : 10.4489/KJM.2012.40.1.054
This study investigates the effect of
-glucans on the growth of Caenorhabditis elegans. Comparison was made among lipopolysaccharide (LPS) and
-glucans extracted from Phellinus baumii, in the presence of peptidoglycans which is available as the major carbon source from OP50, a non-pathogenic strain of Escherichia coli. When the three sources of carbohydrate were added singularly or in mixture to the culture media, a significant level of variation was observed with respect to fecundity. Addition of
-glucans appeared to increase the fecundity. When
-glucans was reinforced in the culture media, the fecundity increased at least 20 percent compared to the OP50-only media which exclusively contains peptidoglycans. In terms of life span, C. elegans showed a modest reduction when treated especially with
-glucans. C. elegans accumulated less fat in the
-glucans containing media different from the OP50 media. Based on the Sudan black staining, fat deposition significantly decreased corresponding to the
-glucans content in the media. On LPS-supplemented media, no difference was observed in fat deposition compared to the normal OP50 media. At the level of motility,
-glucans-treated worms moved more distance as well as LPS-treated worm. They also showed a comparable degree of motility under similar treatment with each source of carbohydrate. In conclusion, LPS and
-glucans, extracted from P. baumii, may not entirely replace the food required for C. elegans; however, it might be utilized as valuable alternative food source which C. elegans use as forms of carbohydrates in stead of peptidoglycan of OP50.
Production of Tyrosinase Inhibitor from Saccharomyces cerevisiae
Jang, In-Taek ; Kim, Young-Hun ; Kang, Min-Gu ; Yi, Sung-Hun ; Lim, Sung-Il ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 60~64
DOI : 10.4489/KJM.2012.40.1.060
Physiological functionalities of culture concentrates from various fungi were investigated. The culture concentrates from Saccharomyces cerevisiae Y277-3 showed the highest tyrosinase inhibitory activity of 42.7%. Among mold physiological functionalities, the culture concentrates from Aspergillus orygae CN20-3-1-4 showed the highest antioxidant activity of 15.8%. The other functionalities of fungi were very low or not detected. The intracellular tyrosinase inhibitor from Saccharomyces cerevisiae Y277-3, which showed the highest physiological functionalities was maximally produced when the strain was cultured in PD broth at
for 24 h.
Soft Rot on Peach Caused by Rhizopus oryzae in Korea
Kwon, Jin-Hyeuk ; Kang, Dong-Wan ; Ha, Jeong-Seok ; Kim, Jin-Woo ; Kwak, Youn-Sig ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 65~68
DOI : 10.4489/KJM.2012.40.1.065
In July and August 2011, a disease suspected to be Rhizopus soft rot was observed on peach (Prunus persica var. vulgaris) at the Wholesale Market for Agricultural Products, Jinju, Korea. The first symptom of soft rot on peach is a water-soaked appearance of the affected tissue. The infected parts later disintegrated into a mushy mass of disorganized cells followed by rapid softening of the diseased tissue. The lesion on peach was rapidly softened and rotted, then became brown or dark brown. Optimum temperature for mycelial growth of the causal fungus on PDA was
and growth was still apparent at
Sporangiophores were 6~20
in diameter. Sporangia were globose and 35~200
in size. The color of sporangia was brownish-grey to blackish-grey at maturity. Sporangiospores were sub-globose, brownish- black streaked and 5~10
in size. Columella were globose to sub-globose and 85~120
in size. On the basis of mycological characteristics, pathogenicity test, and molecular identification, the causal fungus was identified as Rhizopus oryzae Went & Prinsen Geerligs. To our knowledge, this is the first report of soft rot caused by R. oryzae on peach in Korea.
Inhibitory Effect of the Culture Broth of Cordyceps longissima and C. scarabaeicola on Nitric Oxide Production
Lee, Ki-Man ; Lee, Geum-Seon ; Shim, Hong ; Nam, Sung-Hee ; Kang, Tae-Jin ;
The Korean Journal of Mycology, volume 40, issue 1, 2012, Pages 69~71
DOI : 10.4489/KJM.2012.40.1.069
During search for novel bioactive materials from natural resources with the potential as health food and alternative medicine, the culture broth of Cordyceps longissima (CL) J106, J144 and C. scarabaeicola (CS) J94, J123 were prepared, and their effect on cytotoxicity and nitric oxide (NO) production in RAW 264.7 cells were investigated. Whereas the culture broth of CL J144 and CS J123 had cytotoxicity in RAW 264.7 cells, that of CL J106 and CS J94 did not. The culture broth of CL J106 and CS J94 suppressed NO production in RAW 264.7 cells activated with lipopolysaccharide (LPS) at a dose-dependent manner. These results suggest that culture broth, a by-product of Cordyceps, may have active compounds with anti-inflammatory effect. In addition, it appears that their biological activity is dependent on the strains in spite of the same species.