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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 41, Issue 4 - Dec 2013
Volume 41, Issue 3 - Sep 2013
Volume 41, Issue 2 - Jun 2013
Volume 41, Issue 1 - Mar 2013
Selecting the target year
A Taxonomic Study of Genus Melanophyllum in Korea
Seok, Soon Ja ; Jin, Yong Joo ; Kwon, Soon Woo ; Kim, Yang Sup ; Kim, Wan Gyu ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 205~211
DOI : 10.4489/KJM.2013.41.4.205
For the study of species diversity and distribution of Melanophyllum in Korea, M. eyrei and M. haematospermum were identified with unrecorded species and reported species, respectively. Their identification were confirmed based on macroand microscopic descriptions as well as ITS region sequence data. We give a Korean name `Cheongheukjureumbeoseot` for M. eyrei and provided full description for M. haematospermum due to incomplete previous description.
Identification and Characteristics of Mushrooms Grown with Vegetables Cultivated in the Greenhouse
Seok, Soon Ja ; Kim, Wan Gyu ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 212~217
DOI : 10.4489/KJM.2013.41.4.212
Unusual mushrooms were observed in the greenhouses of vegetables Chinese chive, cucumber, melon and Korean dandelion in four locations of Korea in 2012. The mushrooms usually grew with the vegetables cultivated on beds in the greenhouses. Occurrence aspects of the mushrooms in the greenhouses and effect of the mushrooms on growth and quality of the vegetables were investigated. Mushroom samples were collected from the beds in the greenhouses and identified based on their morphological characteristics. The mushroom samples collected from the greenhouse of Chinese chive were identified as Coprinus aokii, those from the greenhouse of cucumber as Cristinia eichleri and Coprinus sp., those from the greenhouse of melon as Leucocoprinus cretaceous, Panaeolus fimicola and Conocybe sp., and those from the greenhouse of Korean dandelion as Conocybe rickenii f. tetrasporique. Out of the seven mushrooms identified, L. cretaceus, C. rickenii and C. eichleri were first found in Korea. The investigation results revealed that the mushrooms were originated from compost or soil used in the beds, and showed that C. aokii caused sooty mold on leaves of Chinese chive, and C. rickenii f. tetrasporique hindered growth of Korean dandelion and caused sooty mold on leaves of the plant. The other mushrooms had no effect on growth and quality of the vegetables.
Characterization of Phosphate Solubilizing Yeasts from Korean Traditional Fermented Foods
Park, In-Cheol ; Kim, Jeong-Seon ; Jung, Joo Ae ; Yoo, Jae-Hong ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 218~224
DOI : 10.4489/KJM.2013.41.4.218
Of 1,100 yeast strains which were isolated from various Korean fermented foods, screened for phosphate solubilization, five strains showed the ability to solubilize tricalcium phosphate. The 26S rDNA domain D1-D2 sequence analysis revealed the identification of strain Y393 and Y524 as Pichia anomala (99.8 and 100% identity, respectively), Y669 as Pichia farinosa (100% identity), Y901 as Candida versatilis (100% identity), and Y1101 as Pichia subpelliculosa (100% identity). All the phosphate solubilizing strains showed mesophilic characteristics. The temperature range for growth of 4 strains was
and P. farinosa Y669 was able to grow up to
. The strain C. versatilis Y907 was able to grow at pH range of 5.0~6.0 and showed halophilic characteristics with tolerance to 15% of NaCl concentration. The Phosphate solubilizing yeast strains were survived well in bed soil for 8 weeks which were maintained densities of
cfu/g. The highest phosphate solubilizing activity was observed in P. subpelliculosa Y1101. It solubilized 697.2 ug/mL of phosphorus from tricalcium phosphate with decrease in pH from 6.8 to 4.37 after 11 days of inoculation.
Cultivation Charateristics of Neolentinus lepideus Strains on Sawdust Media
Kim, Min-Soo ; Jeon, Sung-Min ; Ka, Kang-Hyeon ; Bak, Won-Chull ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 225~230
DOI : 10.4489/KJM.2013.41.4.225
We investigated the cultivation characteristics of Neolentinus lepideus (21 strains derived from Korea and Japan) on Pinus densiflora sawdust medium as follows; period of mycelial growth, fruiting period, productivity of fruit body and color and morphology of fruit body. The periods for mycelial growth and fruiting on the sawdust medium of N. lepideus were 40~65 days and 6~22 days, respectively. It was produced approximately 48.3 g of fresh weight and 9 fruit-bodies per 600 g of sawdust medium. The productivity of fruit body was reduced by scratching off the surface of sawdust medium. We suggest that three strains (KFRI 1199, 1302, 1331) might be good candidates for sawdust cultivation.
Reassessment of the Growth Properties of Mycelium among Cordyceps pruinosa Isolates According to Cultivation Conditions
Kim, Jun Young ; Kwon, Hyuk Woo ; Sung, Gi-Ho ; Choi, Hyung-Kyoon ; Sung, Jae-Mo ; Kim, Seong Hwan ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 231~235
DOI : 10.4489/KJM.2013.41.4.231
To obtain basic data for the better use of Cordyceps pruinosa we reassessed the effect of different medium, culture method, pH, and carbon and nitrogen sources on the mycelial growth properties of four C. pruinosa isolates. The growth of mycelia differed among the four isolates depending on medium type and cultivation days. Among the tested 8 kinds of solid media, the four isolates grew well on PDA and MMMA(mushroom minimal medium agar). While, among the tested 8 kinds of liquid media, all the isolates grew well in SDYM(Sabourand`s dextrose yeast extract medium). The isolates also grew well in the SDYM with pH from 4.0 to 9.5 without any inhibition. One isolate could best grow at pH 8 to 9.5. Regarding the ability of utilizing carbon source, the difference of mycelia growth among the isolates was the most with xylose. Regarding nitrogen source, three isolates could utilize urea which is new fact in this species. These results provide new points on the growth properties of the fungal mycelium which has not been explored before. Overall, this reassessed study concluded that it is necessary to check in advance the growth properties of mycelium when a new isolate of C. pruinosa is expected to be used for application.
The Quantities of Methyl Orsellinate and Sparassol of Sparassis latifolia by Host Plants
Kim, Min-Soo ; Lee, Kyoung-Tae ; Jeon, Sung-Min ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 236~242
DOI : 10.4489/KJM.2013.41.4.236
It is known not only that antifungal compounds such as sparassol, methyl orsellinate (ScI) and methyl-dihydroxy-methoxy-methylbenzoate (ScII) were produced during submerged culture from Sparassis crispa, but also that ScI and ScII were appeared higher antifungal activity than sparassol. The aim of this study, antifungal compounds of Sparassis latifolia were purified from mycelial culture media and identified by using NMR and ESI-MS. Based on HPLC analysis, methyl orsellinate and sparassol were detected at 15 min and 31 min of retention time, respectively. The compounds derived from S. latifolia were classified into four production patterns according to their strains. The strains originated from host plant Larix kaempferi and Pinus koraiensis showed different patterns of compound production, whereas the strains originated from host plant P. densiflora and Abies holophylla showed almost same patterns. There was no correlation between mycelial biomass and compound production. KFRI 645 strain from L. kaempferi exhibited higher methyl orsellinate production (0.170 mg/ml). Sparassol was produced by KFRI 747 from P. densiflora (0.004 mg/ml). Thus, our result revealed the new fact that methyl orsellinate and sparassol have different patterns according to the strains originated from different host plants.
Antioxidant and Anti-inflammatory Effects of Phellinus linteus HN1009K
Kang, Hee-Wan ; Lee, Man Hyee ; Seo, Geon-Sik ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 243~247
DOI : 10.4489/KJM.2013.41.4.243
Novel Phellinus linteus HN1009K (HK1009) has been characterized on speedy mycelial growth and yearly artificial cultivation. In this study, antioxidant and anti-inflammatory activities of ethanol extract from mycelium and fruiting body of P. linteus HN1009K (HK1009) were compared to those of P. linteus (PL, Korea Sangwhang) and P. baumii (PB, Jangsu Sanghwang) as controls. HK1009 extract showed higher DPPH free radical scavenging activity in both mycelial and fruiting body samples than those of PL and PB. However, in xanthine oxidase assay, antioxidant effect was highly measured on mycelial samples of PL and HK1009. Mycelial extract of HK1009 induced high inhibitory effect on LPS-induced nitric oxide (NO) production in RAW264.7 cells comparing to PL and PB without toxicity on the cells. The results suggest that P. linteus HN1009K possesses superior antioxidant activity and anti-inflammatory effects.
Isolation of the Bacterium Pseudomonas sp. HC1 Effective in Inactivation of Tolaasin Produced by Pseudomonas tolaasii
Lee, Chan-Jung ; Yoo, Young-Mi ; Han, Ju-Yeon ; Jhune, Chang-Sung ; Cheong, Jong-Chun ; Moon, Ji-Won ; Suh, Jang-Sun ; Han, Hye-Su ; Cha, Jae-Soon ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 248~254
DOI : 10.4489/KJM.2013.41.4.248
A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.
Manufacture of Pear Marc Nuruk by Aspergillus oryzae and Characteristics of Makgeolli Fermentation
Shin, Ja-Won ; Kim, Young-Hun ; Kim, Jae-Ho ; Na, Kwang-Chul ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 255~260
DOI : 10.4489/KJM.2013.41.4.255
In order to develop new valuable pear marc nuruk and further, reduce environmental pollution by pear marc from pear juice processing, we prepared pear marc nuruk by incubation of Aspergillus oryzae into pear marc containing 50% of moisture at
for 7 days.
-Amylase and glucoamylase activities of the pear marc nuruk were 320.2 IU and 442.8 IU, respectively and its acidic protease activity was showed 142.6 IU. After brewed makgeolli by using the pear marc nuruk, cooked rice and Saccharmyces cerevisiae, its physicochemical characteristics was investigated. Ethanol content of pear marc nuruk-makgeolli was 6.8% after fermentation at
for 10 days and also pear marc makgeolli showed 45.6% of antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activity. In conclusion, pear marc nuruk had high amylase activity and pear marc-makgeolli had also good fermentation characteristics and antihypertensive ACE inhibitory activity. Therefore, it has the potential to become a new nuruk for brewing makgeolli.
Selection of Bacillus amyloliquefaciens CC110 for Biological Control of Cucumber Downy Mildew Caused by Pseudoperonospora cubensis
Lee, Sang Yeob ; Weon, Hang Yeon ; Kim, Jeong Jun ; Han, Ji Hee ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 261~267
DOI : 10.4489/KJM.2013.41.4.261
In order to select antagonists for biological control of downy mildew of cucumber, 126 bacteria were isolated from cucumber plants collected from several locations in Korea. Among them, Five isolates were selected as potential biocontrol agents of cucumber downy mildew using a leaf disc bioassay method. In preventive and curative effect tests, the isolate CC110 was found to be effective to control downy mildew on cucumber showing diseased area by 0% whereas that of control was 15.0~18.0%. A bacterium isolate CC110 was identified as Bacillus amyloliquefaciens subsp. plantarum based on phylogenetic analysis using gyrB gene sequence. The culture liquid of isolate CC110 in TSB media were more effective for the control of the disease than those cultured in LB, NB, and KB media in leaf disk bioassay. when undiluted liquid, two-fold, five-fold diluted culture broth, and undiluted liquid, two-fold, five-fold diluted filtrate of isolate CC110 in TSB media were treated, diseased area of cucumber powdery mildew were 0%, 3.0%, 8.0%, 0%, 4.0% and 7.0%, respectively, whereas diseased area in the control was 21.0%. In the cucumber seedling tests, when the culture broth of isolate CC110 in TSB media was treated, diseased area were 35.0%, whereas that of control was 82.0%. When B. amyloliquefaciens CC110 was treated four times at five-day interval in the vinylhouse test, the control effect of cucumber downy mildew was higher than that treated three at seven-day interval.
Biological Control of Cucumber Powdery Mildew by Bacillus amyloliquefaciens M27
Lee, Sang Yeob ; Weon, Hang Yeon ; Kim, Jeong Jun ; Han, Ji Hee ; Kim, Wan Gyu ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 268~273
DOI : 10.4489/KJM.2013.41.4.268
Bacillus amyloliquefaciens M27 was isolated from the cotton-waste compost for cultivation of oyster mushroom (Pleurotus ostreatus). B. amyloliquefaciens M27 is a biocontrol agent with antagonistic activities against a wide range of fungal pathogens. The aim of this work was to evaluate the possibility of exploiting antagonistic bacteria, B. amyloliquefaciens M27, in the biological control of the cucumber powdery mildew fungus, Podosphaera fusca. In greenhouse tests, the isolate was found to be very effective to control powdery mildew on cucumber leaves showing 4.0% diseased area, whereas diseased area in the control was 80.5%. The filtrate of the isolate cultured on MH and LB media were more effective for control of the disease than those cultured on TSB, NB, and KB media. When two, five, ten, 20, 50 and 100-fold diluted culture broth of isolate on LB media were treated, disease areas were 0%, 0%, 0%, 1.3%, 3.1% and 5.0%, respectively, whereas diseased area in the control was 60.0%. The filtrate of the isolate cultured on LB media was treated to cucumber plants on July, October and December just before the outbreak of the powdery mildew occurred. When 10-fold diluted filtrate of the isolate was treated, control efficacy was 88.9~98.9% in the treated seasons. The results showed that the culture filtrate of B. amyloliquefaciens M27 was very effective to control powdery mildew of cucumber.
Fungicidal Effect of Slightly Acidic Hypochlorous Water against Phytopathogenic Fungi
Song, Jeong Young ; Kim, Narae ; Nam, Myeong Hyeon ; Park, BeomJin ; Whang, Eui-Il ; Choi, Jong Myung ; Kim, Hong Gi ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 274~279
DOI : 10.4489/KJM.2013.41.4.274
Slightly acidic hypochlorous water (SAHW) is well known for having a powerful and broad spectrum antimicrobial activity, and is harmless to the environment and humans. SAHW (pH 5~6.5, 20~30 ppm available chlorine concentration) was generated by electrolysis of dilute solution of HCl (4%) in a chamber of a non-membrane electrolytic cell. Our objective was to determine SAHW has a potential fungicidal activity on some phytopathogenic fungi. Spores of Botrytis cinerea, Colletotrichum acutatum and Phytophthora capsici were not culturable on agar media at approximately 10 seconds after treatment by SAHW. However, inactivation of Penicillium hirsutum was required over 3 min. Dilution of SAHW with sterilized distilled water (SDW) at the ratio of 1:1 (SAHW:SDW) against C. acutatum showed 100% inactivation but, the efficacy in 1:2 decreased until 63.2%. Control value of SAHW was 70.4% against C. acutatum on pepper fruits when applied upto 24 h postinoculation. SAHW has a powerful and wide spectrum antifungal activity and could be applied as a potential alternative to fungicidal agent for control of plant disease.
Note on the New Korean Common Names of Marasmioid Fungi -1. The Genus Marasmius
Ryoo, Rhim ; Antonin, Vladimir ; Ka, Kang-Hyeon ; Shin, Hyeon-Dong ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 280~286
DOI : 10.4489/KJM.2013.41.4.280
47 species was reported in the genus Marasmius in Republic of Korea up to now. 26 of total 47 species previously recorded from Korea were made up a list. Korean common names of 10 new species and 11 species newly recorded in Korea were introduced in study with synoptic key. These names were followed by the Romanization rule to express Korean common name.
Genetic Transformation of the Mycelia of Tremella fuciformis and Changes of Cytotoxicity
Shin, Dong-Il ; Park, Hee-Sung ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 287~291
DOI : 10.4489/KJM.2013.41.4.287
Tremella fuciformis, as one of higher basidiomycetes, can asexually reproduce yeast-like conidium (YLC) cells by budding. We have developed an efficient method to introduce pCambia1300 plasmid containing hph gene into YLC cells using Agrobacterium. This was successful only when YLC cells were wounded by NaOH treatment before co-cultivation. In average, 40~50 transformants were produced out of
YLC cells investigated. The T-DNA transfer was confirmed by PCR. Methanolic extracts from transformants demonstrated different levels of toxicity against SKOV-3 cervical cancer cells.
Gray Mold on Neofinetia falcata Caused by Botrytis cinerea in Korea
Han, Kyung-Sook ; Lee, Seong-Chan ; Lee, Jung-Sup ; Soh, Jae-Woo ; Park, Mi-Jeong ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 292~294
DOI : 10.4489/KJM.2013.41.4.292
In February 2010, the grayish fungus was found on Neofinetia falcata at Namsa-myeon, Yongin city, Gyeonggi-do, Korea. The symptoms start mainly on the leaves and stems, and the infected stems were rotten. Many conidia appeared on the lesions under humid condition. Colonies were grayish brown color and sclerotial formation was observed on potato dextrose agar (PDA). Conidia were mostly ellipsoidal to ovoid in shape, hyaline, one-celled, and
in size. Based on morphological characteristics and pathogenicity, the causal fungus was identified as Botrytis cinerea Pers. This is the first report of gray mold on N. falcata caused by B. cinerea in Korea.
Influence of Fungicide on the Spore Germination and Mycelial Growth of Beauveria bassiana GHA
Kim, Jeong Jun ; Song, Yu Jin ; Han, Ji Hee ; Lee, Sang Yeob ;
The Korean Journal of Mycology, volume 41, issue 4, 2013, Pages 295~300
DOI : 10.4489/KJM.2013.41.4.295
This study was conducted to test effect of fungicides on the spore germination and mycelial growth of BotaniGard
(Beauveria bassiana GHA). Eight fungicides, which are using for plant disease control in cucumber cultivation, with different concentration were mixed with conidia of GHA isolate for 0.5, 2, and 4 hours. The fungicides trifluminazol, fenarimol, ethaboxam, copper hydroxide and tetraconazol did significantly not inhibit on spore germination and mycelial growth. The trifluminazol, propineb and tetraconazol on high concentrations inhibited the mycelial growth of GHA. However, mixing time of fungicide with conidia of GHA did not affect in spore germination and mycelial growth. These results suggest that fungicide effect needed be checked before mycopesticide is applied in the field.