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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 42, Issue 4 - Dec 2014
Volume 42, Issue 3 - Sep 2014
Volume 42, Issue 2 - Jun 2014
Volume 42, Issue 1 - Mar 2014
Selecting the target year
Maintenance of Filamentous Fungi in Korean Agricultural Culture Collection (KACC)
Shin, Myeong-Suk ; Hong, Seung-Beom ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 97~103
DOI : 10.4489/KJM.2014.42.2.97
A total of 7039 strains of filamentous fungi are preserved in Korean Agricultural Culture Collection (KACC). The 4065 strains (58%) of them, which produce many spores in cultivation on proper media, are preserved with freeze-drying method. They are also preserved with liquid nitrogen and deep-freezer storage in order to minimize loss by death. Aspergillus, Penicillium, Lichtheimia, Mucor, Rhizopus, etc. which are common in surrounding environments, are included in this category. The others which do not produce spores, or produce few spores in vitro, are preserved with liquid nitrogen, deep-freezer and mineral oil storage. Phytophthora, Pythium, Cercospora, Septoria, Rhizoctonia, etc. are included in this category. The authors also introduced various fungal preservation methods and provided detailed preservation procedures that are used in KACC.
Species Diversity of Spontaneous Mushrooms on Jeju Island
Ko, Pyung Yeol ; Seok, Soon Ja ; Lee, Hyang Burm ; Ko, Hyo Soon ; Jeun, Yong Chull ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 104~132
DOI : 10.4489/KJM.2014.42.2.104
This study was conducted to survey the diversity and distribution of mushrooms growing spontaneously on Jeju Island in the last eight years. Approximately 2,000 specimens of mushrooms were collected in Gotjawal, Hallasan Natural Park, and Oreum, the habitats where a rich diversity of mushrooms was expected, from 2006 to 2013. They were identified as 74 families, 213 genera and 511 species. Thus, including 69 families, 99 genera and 561 species from the previous study conducted in 2005, a total of 83 families, 257 genera and 740 species of wild mushrooms have been recorded. This study would support new information for fungal flora in Korea and preliminary data for further studies on mushroom diversity.
Community Changes of Ectomycorrhizal Fungi by Thinning in a Forest of Korea
Choi, Jae-Wook ; Lee, Eun-Hwa ; Eo, Ju-Kyeong ; Koo, Chang-Duck ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 133~137
DOI : 10.4489/KJM.2014.42.2.133
This study was conducted to investigate the effect of thinning on ectomycorrhizal fungal communities in a forest. Ectomycorrhizal root tips were collected from forest soils in thinning and non-thinning sites and identified using morphological characteristics and molecular analysis of ITS rDNA sequences. As a result, species richness of ectomycorrhizal fungi was significantly increased and ectomycorrhizal fungal community composition was changed by thinning. These results suggest that forest management such as thinning, could be an important factor affecting mutualistic relationships and belowground microorganisms in forest ecosystems.
Molecular Phylogenetic Analysis of Botrytis cinerea Occurring in Korea
Back, Chang-Gi ; Lee, Seung-Yeol ; Jung, Hee-Young ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 138~143
DOI : 10.4489/KJM.2014.42.2.138
Several isolates were collected from apple, pepper, strawberry, cucumber and tomato having typical gray mold symptoms. All the isolates were identified as Botrytis cinerea by using morphological characteristics and PCR-RFLP method. It was difficult to analyze the phylogenetic relationship of these isolates by using ITS region, HSP60 and G3PDH because these genes were highly homologous in their nucleotide in inter-species of B. cinerea and intra-species of genus Botrytis. However, phylogenetic analysis using combined sequences (RPB2, HSP60 and G3PDH genes) clearly showed that all isolate of B. cinerea were different from Botrytis spp. Furthermore, it was also confirmed that strawberry isolate was distantly related to apple, pepper, cucumber and tomato isolates that were closely related to each other in nucleotide level.
Changes of Nutrients in Media and Mycelia on Liquid Spawn Culture of Lentinula edodes
Shim, Kyu-Kwang ; Yoo, Young-Jin ; Koo, Chang-Duck ; Kim, Myung-Kon ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 144~149
DOI : 10.4489/KJM.2014.42.2.144
Lentinula edodes liquid spawn growth under explosive aeration (supplying air with tiny bubbles) and soybean meal addition to liquid culture medium were investigated in terms of mycelial growth and residual free sugar content. The two treatments were effective for homogeneous culturing of mycelial spawn and for separating colonies during multiplication after an exponential growth period without limiting sustaining nitrogen nutrients. The mycelial growth and carbon dioxide concentration were greatest on the 13th day since the inoculation. At 12th day, however, free sugars were almost depleted in the upper part of the liquid medium. Total nitrogen content within precipitated mycelia was the highest at the 13th day. Chitin and sucrose contents in the mycelia were the highest at the 18th day, but ergosterol content became highest at 22 days. These results suggest that Lentinula edodes liquid spawn is ready in 18 days after inoculation.
Mycelial Growth of Ectomycorrhizal Fungi by Different Carbon Sources in Liquid Culture
Jeon, Sung-Min ; Jeon, Ha-Saem ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 150~158
DOI : 10.4489/KJM.2014.42.2.150
Four ectomycorrhizal fungi were tested for their ability to utilize seven carbon sources in modified Melin-Norkrans medium. After 60 days of mycelial culture, the highest mycelial growth in Hygrophorus russula (KFRI 1987), Sarcodon aspratus, Leccinum extremiorientale (KFRI 1194), and Tricholoma matsutake (KFRI 1256) was observed with use of dextrin used as a carbon source. H. russula, S. aspratus (KFRI 1676), and L. extremiorientale showed the lowest mycelial growth on nutrient medium with pectin. The utilization of homoglycans (starch, dextrin) in seven strains (except for T. matsutake KFRI 1256) was higher than that of heteroglycan (pectin). The final pH values of all culture media were decreased by pH 1.1~3.0 compared with the initial pH values of culture media. The dominant color of mycelia was white and varied according to the carbon sources (yellow, brown, and purple) in some strains. A single colony was observed in L. extremiorientale cultured in liquid media containing four or five different types of carbon sources, whereas multiple colonies were formed in liquid media containing six different types of carbon sources by six strains.
Multiplex Simple Sequence Repeat (SSR) Markers Discriminating Pleurotus eryngii Cultivar
Im, Chak Han ; Kim, Kyung-Hee ; Je, Hee Jeong ; Ali, Asjad ; Kim, Min-Keun ; Joung, Wan-Kyu ; Lee, Sang Dae ; Shin, HyunYeol ; Ryu, Jae-San ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 159~164
DOI : 10.4489/KJM.2014.42.2.159
For development of a method for differentiation of Pleurotus eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotyping results. PCR using each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in the range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pairgroup method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into two clusters. Cluster I and II were comprised of four and eight cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivars and strains with 21 alleles and a PIC value of 0.9090. These results might be useful in providing an efficient method for the identification of P. eryngii cultivars with separate PCR reactions.
Germination and Antioxidant Activity of Korean Oak Pollen Treated with Medicinal Mushrooms
Hong, In-Pyo ; Woo, Soon-Ok ; Han, Sang-Mi ; Yeo, Joo-Hong ; Cho, Mi-Lan ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 165~169
DOI : 10.4489/KJM.2014.42.2.165
In this study, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized oak pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of oak pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in oak pollen germinated with A. mellea. The total polyphenol content of the lyophilized oak pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized oak pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized oak pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of acorn pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from oak pollen inoculated with A. mellea.
Characteristics of Two Unrecorded Yeasts from Wild Flowers in Ulleungdo, Korea
Hyun, Se-Hee ; Min, Jin-Hong ; Lee, Hyang Burm ; Kim, Ha-Kun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 170~173
DOI : 10.4489/KJM.2014.42.2.170
Two unrecorded yeasts, Meyerozyma caribbica UL5-1 and Pichia silvicola UL6-1 were screened from 58 yeasts which were isolated from wild flowers in Ulleungdo in Gyeongsangbuk-do, Korea. The morphological and cultural characteristics of these unrecorded yeasts were investigated. Both yeasts were oval in shape and formed pseudomycelia. P. silvicola UL6-1 formed ascospore, but M. UL5-1 did not. P. silvicola UL6-1 and M. caribbica UL5-1 also grew in vitamin-free medium and 5% NaCl-containing yeast extract-peptone-dextrose medium. The two unrecorded yeasts assimilated glucose, galactose, xylose, cellobiose, trehalose, glycerol and sorbitol, and also fermented glucose, fructose and mannose. The supernatant of both M. caribbica UL5-1 and P. silvicola UL6-1 showed high antihypertensive angiotensin I-converting enzyme inhibitory activity of 84.2% and 82.6%, respectively. Cell-free extract of P. silvicola UL6-1 also showed very high anti-diabetic
-glucosidase inhibitory activity (85.8%).
Diversity of Endophytic Fungi Isolated from Leaves of Coniferous Trees on Mt. Minjuji, Korea
Kim, Chang-Kyun ; Eo, Ju-Kyeong ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 174~177
DOI : 10.4489/KJM.2014.42.2.174
We investigated the biodiversity of endophytic fungi isolated from leaves of three coniferous species (Pinus densiflora, P. koraiensis, Larix kaempferi) on Mt. Minjuji, Korea. A total of 39 isolates were discovered from 17 host plants and their homology with the ITS region was searched. They were then identified as 22 taxa; 64% of fungal isolates belong to Sordariomyetes, 33% of isolates to Leotiomycetes, and 3% isolates to Agaricomycetes.
Yeasts Associated with Fruits and Blossoms Collected from Hanbat Arboretum, Daejeon, Korea
Hyun, Se-Hee ; Min, Jin-Hong ; Kim, Seon-A ; Lee, Jong-Soo ; Kim, Ha-Kun ;
The Korean Journal of Mycology, volume 42, issue 2, 2014, Pages 178~182
DOI : 10.4489/KJM.2014.42.2.178
Yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various fruits and blossoms are limited. We collected different kinds of blossoms and unripened fruits from Hanbat arboretum, Daejeon, Korea at the year of 2013. Yeasts were isolated by plating of suspensions prepared for collected samples onto YPD medium containing antibiotics. BLAST searches were subsequently performed for the comparison of the partially determined sequences of D1/D2 domain of 26S rDNA. As a result, we isolated 57 yeast strains of 31 species from 29 different kinds of blossoms and 6 kinds of fruits samples. We found huge differences in yeast flora depending on the sample collection season.