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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 42, Issue 4 - Dec 2014
Volume 42, Issue 3 - Sep 2014
Volume 42, Issue 2 - Jun 2014
Volume 42, Issue 1 - Mar 2014
Selecting the target year
Biological Characterization of Marssonina coronaria Infecting Apple Trees in Korea
Back, Chang-Gi ; Jung, Hee-Young ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 183~190
DOI : 10.4489/KJM.2014.42.3.183
Apple blotch is a major apple disease and recently it causes critical economic losses in apple orchards in Korea. In this review, we described the history of apple blotch researches, ecology and life cycle of Marssonina coronaria, cultural and molecular characteristics and simple isolation method as well. Furthermore, characteristic of apple blotch like symptoms, its cause still unknown, occurs frequently in cv. "Fuji" was described in detail.
Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
Kim, Min Sik ; Kim, Sinil ; Ha, Byeong-Seok ; Park, Hye-Young ; BaeK, Seong-Yeol ; Yeo, Soo-Hwan ; Ro, Hyeon-Su ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 191~200
DOI : 10.4489/KJM.2014.42.3.191
Nuruk samples collected from various regions in Korea were investigated in terms of fungal contents and diversity. In measurement of colony forming unit (CFU) in Nuruk suspensions on DRBC agar, Nuruk samples MS4, MS8, and MS10 were among the highest fungal density, with
) were among the samples showing the highest fungal density. CFU per 20 mg Nuruk, respectively. The majority of fungal components were yeasts, including Pichia anomala, P. kudriavzevii, Kluyveromyces marxianus, and Saccharomycopsis fibuligera, whereas Aspergillus oryzae and Rhizopus oryzae, the representative Nuruk fungi, were predominant only in the low fungal density Nuruks (MS2, MS5, and MS11). Saccharification capability of the fungal isolates was assessed by measurement of amylase activity in the culture broth. The highest amylase activity was found in A. niger and A. luchuensis, followed by S. fibuligera. A. oryzae and R. oryzae showed fair amylase activity but significantly lower than those of the three fungal species. R. oryzae was suggested to play an additional role in degradation of
-glucan in crop component of Nuruk since R. oryzae was the only fungus that showed
-glucanase activity among the fungal isolates. To confirm the safety of Nuruk, aflatoxigenicity of the isolated Aspergillus was estimated using the DNA markers norB-cypA, aflR, and omtA. All of the isolates turned out to be non-aflatoxigenic as evidenced by the deletion of gene markers, norB-cypA and aflR, and the absence of aflatoxin in the culture supernatants shown by TLC analysis.
Isolation and Physiological Functionality of Yeasts from Wild Flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea
Hyun, Se-Hee ; Han, Sang-Min ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 201~206
DOI : 10.4489/KJM.2014.42.3.201
Sixty one yeast strains of 21 species were isolated from wild flowers in Gogunsanyeoldo, including Seonyudo of Jeollabuk-do, Korea, and identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA using BLAST. Among them, Cryptococcus sp. including C. aureus SY1-4 were found to be dominant, and Metschnikowia sp. including M. reukaufii SY20-1 and Rhodotnula sp. such as R. ingeniosa SY1-1 were also abundantly isolated. Some physiological functionalities of the culture broth and cell-free extracts from 61 yeast strains were determined. Supernatant from Metschnilowia reukaufii SY44-6 showed anti-gout xanthine oxidase inhibitory activity of 49.6% and whitening tyrosinase inhibitory activity of 38.4%, respectively.
Physiological Activities of Water Extract and Solvent Fractions of an Edible Mushroom, Pholiota adiposa
Kim, Jun-Ho ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 207~212
DOI : 10.4489/KJM.2014.42.3.207
This study was conducted in order to investigate the physiological activities, including antioxidative, fibrinolytic, thrombin inhibitory, and
-glucosidase inhibitory activities of the water extract and solvent fractions isolated from Pholiota adiposa. The antioxidative activities of the water extract and water fraction were 57.57% and 48.27%, respectively. The fibrinolytic activity was strong only in the ethyl acetate fraction at 0.70 plasmin units/mL. The ethyl acetate fraction showed high thrombin inhibitory activity, and a-glucosidase inhibitory activity at 77.67% and 89.32%, respectively. The ethyl acetate fraction hydrolyzed both
subunits of human fibrinogen, but did not show reactivity for the
form of human fibrinogen. Fibrinolytic activity of the ethyl acetate fraction was not decreased by heating for 10 min at
Decolorization Efficiency of Different Dyes by Extract from Spent Mushroom Substrates of Pleurotus eryngii
Lim, Seon Hwa ; Kwak, A Min ; Min, Gyeong Jin ; Kim, Sang Su ; Lee, Sang Yeop ; Kang, Hee Wan ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 213~218
DOI : 10.4489/KJM.2014.42.3.213
Water extract from spent mushroom substrates (SMS) of Pleurotus eryngii was utilized in decolorization of eight synthetic dyes and wastewater from a textile factory. High laccase activity was detected in the extract of P. eryngii (SMSE). The SMSE showed that decolorization rate was 34~93% after 24 h incubation without any mediator on eight dyes including Rit-blue and Rit-red used in fiber dyeing. Dye decolorization rate more than 90% was observed on bromophenol blue and remazol brilliant blue R (RBBR). Dye in textile wastewater was decolorized at room temperature after three days by addition of P. eryngii SMSE. The results suggest that biological decolorization of dyes using the P. eryngii SMSE can be used as environmental friendly materials.
Pseudomonas azotoformans HC5 Effective in Antagonistic of Mushrooms Brown Blotch Disease Caused by Pseudomonas tolaasii
Lee, Chan-Jung ; Yoo, Young-Mi ; Han, Ju-Yeon ; Jhune, Chang-Sung ; Cheong, Jong-Chun ; Moon, Ji-Won ; Gong, Won-Sik ; Suh, Jang-Sun ; Han, Hye-Su ; Cha, Jae-Soon ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 219~224
DOI : 10.4489/KJM.2014.42.3.219
A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system.
Biological Control of Lettuce Sclerotinia Rot by Bacillus subtilis GG95
Lee, Hyun-Ju ; Kim, Jin-Young ; Lee, Jin-Gu ; Hong, Soon-Sung ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 225~230
DOI : 10.4489/KJM.2014.42.3.225
Sclerotinia sclerotiorum, a plant pathogenic fungus, can cause serious yield and quality losses in the winter lettuce field. For biological control of S. sclerotiorum, soil-born microorganisms that inhibit the mycelia growth of S. sclerotiorum and Fusarium oxysporum were isolated from diseased soil. Among the isolates, bacterial isolate, GG95, which was identified as Bacillus subtilis according to the morphological, physiological characteristics and by 16S rRNA similarity, showed the highest level of inhibitory activity. The growth conditions for B. subtilis GG95 were optimized in TSB media (pH 7) by culturing at
for 24 hrs. Maltose or fructose and peptone were selected as the best carbon and nitrogen sources, respectively. Greenhouse experiment was performed to test effectiveness of B. subtilis GG95 in the control sclerotinia rot. Drench application (
, 3 times) of the bacterial culture broth to lettuce showed an effectiveness value of 88%, suggesting that B. subtilis GG95 would be a promising biocontrol agent for control of sclerotinia rot.
Mycological Characteristics of Nine Unrecorded Yeasts from Flowers in the Orchard of Yesan-gun, Chungcheongnam-do and Hanbat Arboretum in Daejeon City, Korea
Han, Sang-Min ; Hyun, Se-Hee ; Shin, Ja-Won ; Kim, Ha-Kun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 231~234
DOI : 10.4489/KJM.2014.42.3.231
Six unrecorded yeasts, Cryptococcus festucosus 41-3, Cryptococcus heveanensis 56-4, Debaryomyces nepalensis 95-4, Issatchenkia occidentalis 142-1, Dioszegia zsoltii 39-1, and Kwoniella europala 47-2 were screened from 108 yeasts isolated from flowers and fruits in orchards of Yesan-gun, Chungcheongnam-do, Korea. The morphological and cultural characteristics of these unrecorded yeasts were investigated. They had various shapes, including ellipsoidal, globose, and oval, and also had budding mode in vegetable reproduction, except I. occidentalis 142-1 (fission mode). K. europaea 47-2 only formed pseudomycelium. D. zsoliti 39-1 did not grow in yeast extract-malt extract medium, potato dextrose medium, and vitamin-free medium. C. festucosus 41-3 grew well in 5% NaCl-containing yeast extract-peptone-dextrose medium and had a growth pH range of 7.0~10.0. Three unrecorded yeasts Ogataea polymorpha HB45-1, Rhodotonula hinnulla HB62-2, and Cryptococcus rajasthanensis HB80-4 were screened from 51 yeasts isolated from flowers in Hanbat arboretum in Daejeon city, Korea. They were globose in shape and did not form pseudomycelium. In addition, O. polymorpha HB45-1 and C. rajasthanensis HB80-4 had budding mode in vegetable reproduction. All of them grew well in vitamin-free medium and C. rajasthanesis HB80-4 also grew in 50% glucose and 5% NaCl-containing YPD medium.
A New Report on Oidiodendron flavum Isolated from Field Soil in Korea
Adhikari, Mahesh ; Kim, Sangwoo ; Yadav, Dil Raj ; Babu, Anam Giridhar ; Kim, Changmu ; Lee, Hyang Burm ; Lee, Youn Su ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 235~238
DOI : 10.4489/KJM.2014.42.3.235
Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region (ITS) of rDNA and morphological characteristics. Based on phylogenetic analysis of ITS and morphological characteristics, the species has not been previously reported in Korea.
Four Species of Endophytic Fungi Isolated from Leaves of Woody Plants in Mt. Hambaek
Eo, Ju-Kyeong ; Lee, Bong-Hyung ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 239~242
DOI : 10.4489/KJM.2014.42.3.239
In this study, endophytic fungi were isolated from leaves of four species of woody plants, Acer tagmentosum, Larix kaempferi, Abies holophylla, and Pinus koraiensis, on Mt. Hambaek, Gangwondo, Korea. The endophytic fungi were identified using morphological and sequences analysis of ITS regions. The fungal endophytes were identified as Talaromyces radicus, Myceliophthora verrucosa, Cryptosporiopsis diversispora, and Sphaerulina berberidis, which are the first record in Korea. The morphological and molecular phylogenetic characteristics of each strain were described.
Monotropoid Mycorrhizal Characteristics of Monotropa uniflora (Ericaceae) Collected from a Forest in Korea
Lee, Eun-Hwa ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 243~246
DOI : 10.4489/KJM.2014.42.3.243
The roots of Monotropa uniflora were collected from a forest in Korea. Morphological characteristics of monotropid mycorrhizas of the plants were determined. Thick mantles covered the roots and fungal pegs inside the epidermal cells of the roots were observed. Fungal symbionts were identified by sequence analysis of internal transcribed spacer region. Phylogenetic analysis based on the sequences demonstrated that the fungus was the most closely related to Russula heterophylla. The result support the strong specificity between M. uniflora and Russula species.
The Effect of Shading Treatment on the Uniform Primordium Formation and Development of Pleurotus ostreatus during Incubation
Jung, Yun-Kyoung ; Lee, Yun-Hae ; Baek, Il-Sun ; Chi, Jeong-Hyun ; Kang, Young-Ju ;
The Korean Journal of Mycology, volume 42, issue 3, 2014, Pages 247~252
DOI : 10.4489/KJM.2014.42.3.247
The study was conducted to investigate the effect of shading treatment on the uniform primordium formation and development of fruit body during incubation of major cultivated oyster mushrooms (Pleurotus ostreatus). Oyster mushrooms cultivars Suhan No. 1 and Gonji No. 7 were incubated while shading during different times in the incubation room wherein temperature of
was maintained. Substrate temperature during incubation was increased to
in 11~12 days for Suhan No. 1 and to
in 11~12 days for Gonji No. 7 regardless of shading times. The
generation was the highest as 9~11% for Suhan No. 1 and 9~10% for Gonji No. 7 at the time of temperature increase the highest, while
contents became decreased, thus constructed good environment for the growth of hyphae. Therefore, ratio of ununiformal primordia formation was reduced by 20% as against control for Suhan No. 1 and by 13% for Gonji No. 7 when shading was applied from
day of inoculation with no difference in fruit body yield.