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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 42, Issue 4 - Dec 2014
Volume 42, Issue 3 - Sep 2014
Volume 42, Issue 2 - Jun 2014
Volume 42, Issue 1 - Mar 2014
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Biodiversity and Distribution of Arbuscular Mycorrhizal Fungi in Korea
Eo, Ju-Kyeong ; Park, Sang-Hee ; Lee, Eun-Hwa ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 255~261
DOI : 10.4489/KJM.2014.42.4.255
In this study, we summarized previous studies on diversity and distribution of arbuscular mycorrhizal fungi (AMF) for last 30 years in Korea. According to a review of the literature concerning AMF in Korea, 14 genera and 89 species have been recorded. Host plants for AMF are very diverse and include crop species and woody plants in natural forests. Based on the achievements of the last 30 years of study on AMF, we anticipate that relatively more intensive studies of the functional and genetic diversity of AMF will be conducted.
Identification and Characterization of Paraconiothyrium brasiliense from Garden Plant Pachysandra terminalis
Choi, Min Ah ; Park, Seung Jun ; Ahn, Geum Ran ; Kim, Seong Hwan ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 262~268
DOI : 10.4489/KJM.2014.42.4.262
A fungal isolate DUCC5000 from a garden plant Pachysandra terminalis was identified as Paraconiothyrium brasiliense based on the results of morphological and molecular studies. The fungus formed brown to black conidiomata of (0.2-0.7)-2(-3.5) mm singly or as a group on PDA. Conidia measured
in size, hyaline, ellipsoid to short-cylindrical, and rounded at both ends. The internal transcribed spacer (ITS) DNA of the isolate shared 100% nucleotide sequence homology with those of known P. brasiliense isolates. Phylogenetic tree inferred from the ITS sequence analysis showed that the DUCC5000 isolate formed a clade with known isolates of P. brasiliense. The fungal mycelia grew better on oatmeal agar than on MEA and PDA. On PDA media under various pH conditions, fungal mycelial growth was observed at pH 9. Colony morphology of the fungus tended to alter depending on the kinds of nutrient media and pH condition. On chromagenic media, the fungus demonstrated its ability to produce extracellular enzymes including amyalse, avicelase,
-glucosidase, protease, and xylanase. However, in pathogenicity testing, no disease symptoms were observed on the leaves of P. terminalis. This strain is the first report on P. terminalis in Korea.
Endophytic Fungal Diversity Isolated from the Root of Halophytes in Taean Peninsula
You, Young-Hyun ; Lee, Myung-Chul ; Kim, Jong-Guk ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 269~275
DOI : 10.4489/KJM.2014.42.4.269
Halophytes of seven species, Carex scabrifolia Steud., Limonium tetragonum Bullock, Salicornia europaea L., Suaeda glauca Bunge, Suaeda japonica Makino, Suaeda maritima Dumort., and Triglochin maritimum L. were collected from the Taean Peninsula. Thirty-seven endophytic fungi were isolated from the root of halophytes, and analyzed using the DNA sequences of internal transcribed spacers (ITS). The diversity of all endophytic fungi was analyzed using diversity indices. The endophytic fungi associated with the halophytes belonged to seven orders; Eurotiales (78%), Capnodiales (5%), Hypocreales (5%), Agaricales (3%), Corticiales (3%), Glomerellales (3%), and Pleosporales (3%). At the phylum level, the endophytic fungi were composed of Ascomycota and Basidiomycota. At the genus level, Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, Phanerochaete, Schizophyllum, Talaromyces, and Verticillium were confirmed. Among them, Penicillium was the most abundant in the roots of the halophytes. This study analyzed the distribution and diversity of endophytic fungi on halophytes in the Taean Peninsula.
Identification of Fungi Isolated from Cheongdo-Banshi (flat persimmon) Stored in a Refrigerator
Bang, Narae ; Ha, Sango ; Kim, Dae-Ho ; Kim, Seon-Hwa ; Hong, Seung-Beom ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 276~281
DOI : 10.4489/KJM.2014.42.4.276
Nine strains of fungi which showed different colonies were isolated from Cheongdo-Banshi (flat persimmon) stored in a refrigerator. Based on morphological and molecular characteristics, they were identified as Botrytis cinerea (n=4), Penicillium expansum (n=2), and Rhizopus delemar (n=1). Of these, B. cinerea and P. expansum occurred frequently but R. delemar occurred rarely. This report of P. expansum and R. delemar on persimmon is the first time in Korea.
Identification and Characterization of Aspergillus oryzae Isolated from Soybean Products in Sunchang County
Lim, Eunmi ; Lee, Ji Young ; Elgabbar, Mohammed A. Abdo ; Han, Kap-Hoon ; Lee, Bo-Soon ; Cho, Yong Sik ; Kim, Hyoun-Young ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 282~288
DOI : 10.4489/KJM.2014.42.4.282
In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify Aspergillus oryzae from fungal isolates. Ten fungal isolates were identified with
-tubulin gene. According to the sequences of
-tubulin gene, ten fungal isolates were identified as A. oryzae/flavus complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of A. oryzae and A. flavus strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of norB and cypA intergenic region, in which a deletion was recognized relative to A. flavus and A. parasiticus. The amplicon size of the ten fungal isolate strains was smaller than those of A. flavus and A. parasiticus, but the same as that of the reference A. oryzae strain. These results indicated that the ten isolates should be identified as A. oryzae. The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the A. oryzae isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.
Higher Fungal Flora from the Worldcup Park in Seoul
Seok, Soon-Ja ; Jin, Yong-Ju ; Yoo, Ki-Bum ; Kwon, Soon-Woo ; Park, In-Cheol ; Lee, Sang-Yeob ; Kim, Yang-Sup ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 289~305
DOI : 10.4489/KJM.2014.42.4.289
This survey was conducted in order to clarify the species diversity and distribution of mushrooms at Worldcup Park in Seoul, from May to November 2010 and 2014. In the survey, a total of 98 taxa, 98 species, 86 genera, 38 families, 12 orders, three classes, and two phyla were identified. A total of 250 specimens of Mushrooms were collected from Pyonghwa Park and 63 species, 71 genera, 36 families, 13 orders, three classes, and two phyla were identified. Coprinus atramentarius and Psathyrella candolleana were high in frequency, as ten times and nine times, respectively. In Noeul Park, 102 specimens were collected, 41 species, 42 genera, 19 families, 19 orders, two classes, and two phyla were identified. In Noeul Park mostly layed with lawn, Agrocybe semiorbicularis and Agaricus campestris were high in frequency, as ten times and nine times, respectively. There were no mushrooms in succession during three years. In Haneul Park, a total of 128 specimens of mushrooms were collected. 49 species, 57 genera, 30 families, 11 orders, three classes, and two phyla were identified. Agrocybe semiorbicularis and Phallus rugulosus were high in frequency, as four times. In Nanjicheon Park, a total of 68 specimens of mushrooms were collected. 31 species, 34 genera, 30 families, nine orders, one class, and one phylum were identified. Coprinus atramentarius and Psathyrella velutina were high in frequency, as three times. Investigation on the diversity of mushrooms at the Worldcup Park, piled up with the city's waste, will be of the great significance in the aspect of the ecological change. All specimens examined in this work are preserved in HCCN (Herbarium Conservation Center of National Academy of Agricultural Science).
Notes on Some Unrecorded Species of Arbuscular Mycorrhizal Fungi Collected from Rhizospheres of Plants in Korea
Lee, Eun-Hwa ; Lee, Jeong-Yoon ; Eo, Ju-Kyeong ; Ka, Kang-Hyeon ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 306~311
DOI : 10.4489/KJM.2014.42.4.306
In this study, arbuscular mycorrhizal fungi (AMF) were isolated and cultivated from rhizospheres of various plants in Korea. Five species belonging to four genus were identified based on spore morphology and 18S rDNA sequence analysis: Acaulospora cavernata Claroideoglomus luteum, Diversispora aurantium, D. trimurales, Rhizophagus irregularis. These fungal species have not been cultivated or reported to date in Korea, Morphological description and phylogenetic analysis for each species were presented. This result could be useful for research of AMF diversity in Korea.
Mycelial Growth and in vitro Ectomycorrhizal Synthesis on Pinus densiflora Seedlings of Tricholoma bakamatsutake in Korea
Jeon, Sung-Min ; Ka, Kang-Hyeon ; Hong, Ki-Sung ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 312~321
DOI : 10.4489/KJM.2014.42.4.312
Tricholoma bakamatsutake is one of the edible ectomycorrhizal mushrooms as an allied species of Tricholoma matsutake. This is the first report on physical characteristics of T. bakamatsutake strains collected from Quercus mongolica forests in Korea. The pure cultures of these strains were isolated from the tissues of fruit bodies and the culture characteristics were investigated under different conditions (media, temperatures, nitrogen sources). Most strains showed the highest mycelial growth on potato dextrose agar (PDA) at 20 or
. Two strains of T. bakamatsutake preferred the ammonium-form rather than the nitrate-form as an inorganic nitrogen source. T. bakamatsutake showed significantly slower mycelial growth when compared with T. matsutake from a Korean forest, although the optimum culture conditions for the two allied species were similar. We also tested the ability to form mycorrhizae as well as cellulase activity of T. bakamatsutake. All strains showed cellulase activity on a carboxymethylcellulose (CMC) agar plate. The mycorrhizae on axenic Pinus densiflora seedlings were formed by two strains of T. bakamatsutake after 3 or 8 months of inoculation. P. densiflora seedlings inoculated with T. bakamatsutake had a much higher biomass than un-inoculated seedlings.
Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes
Jeong, Yeun Sug ; Ka, Kang-Hyeon ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 322~327
DOI : 10.4489/KJM.2014.42.4.322
Subculture is the most common method for preservation fungi, but has a disadvantage of accumulation of spontaneous mutations during the repeated subculture. To reduce the subculture frequency, the effect of preservation period at
in a slant culture was examined on the mycelia growth and lignocellulolytic enzyme activities of various basidiomycetes. Mushrooms, including Stereum ostrea, Coprinellus micaeus, Trametes versicolor, Hypholoma fasciculare, Wolfiporia extensa, Pleurotus pulmonarius, Piptoporus betulinus and Ganoderma applanatum were not affected by the preservation period more than two years, indicating that they can be maintained by subculture every two years. Some other tested fungal strains showed a significant decrease in both viability and enzyme activity when they were maintained for two years, suggesting that they should be subcultured at least once in a year. A little correlation was found between the recovery of mycelial growth and extracellular enzyme activity. In conclusion, mycelial activity and enzyme activity according to storage period is expected to be a way of deciding on subculture times for fungal preservation.
Selection of Carbon, Nitrogen Source and Carrier for Mass Production of Beauveria bassiana
Kim, Jeong Jun ; Han, Ji Hee ; Lee, Sangyeob ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 328~332
DOI : 10.4489/KJM.2014.42.4.328
For mass production of entomopathogenic fungus Beauveria bassiana 149, isolated from moth larva, by two-phase fermentation, we performed selection of carbon and nitrogen sources for liquid culture and examined solid fermentation on carrier, ingredient, temperature, and water content. Spore production with rice powder, corn powder, and starch from sweet potato was higher than that of sucrose and dissolvable starch for liquid fermentation as first-phase fermentation. As a nitrogen source, addition of peptone and yeast powder showed higher spore production than
, fish powder, and soybean powder. The isolate produced more conidia in sawdust + wheat bran + corn powder, sawdust + wheat bran and rice shell + wheat bran as carrier and ingredient than vermiculite as carrier. Conidia production of B. bassiana 149 in solid-phase fermentation was twice higher at 30 than 20. Conidia yield was higher at 60% and 70% water content (
) than 40% and 50% (
Screening of Antagonistic Bacteria having Antifungal Activity against Various Phytopathogens
Yang, Hee-Jong ; Jeong, Su-Ji ; Jeong, Seong-Yeop ; Jeong, Do-Youn ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 333~340
DOI : 10.4489/KJM.2014.42.4.333
The aim of this study was to isolate a potential multifunctional biocontrol agent from bacteria for control of multiple plant diseases as an alternative to fungicides. A total of 201 strains were isolated from soil undamaged by repeated cultivation in Sunchang and their ability to produce antibiotics, siderophores and extracellular enzymes such as protease, cellulase and amylase was investigated. Selected strain SCS3 produced cellulose, protease and amylase. This strain also produced siderophores and showed excellent antifungal activity against various phytopathogens. SCS3 was identified as Bacillus subtilis using 16S rRNA sequencing, and named Bacillus subtilis SCS3. Finally, physiological and biochemical characteristics of B. subtilis SCS3 were examined. From the results, B. subtilis SCS3 was found to be a useful multifunctional biocontrol agent against various phytopathogens.
A New Record of the Genus Camarops (Boliniaceae) in Korea
Kim, Changmu ; Lee, Jin Sung ; Lim, Young Woon ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 341~343
DOI : 10.4489/KJM.2014.42.4.341
Camarops ustulinoides (Henn.) Nannf., belonging to the family Boliniaceae (Order Boliniales), is reported as new to Korea. The morphological characteristics and molecular phylogenetic analysis based on the nucLSU region sequence confirm the recognition of this taxon. Illustrated descriptions of C. ustulinoides are provided.
Eight Previously Unreported Species of Fungi Identified in Mt. Manggyeong, Korea
Lee, Jin Sung ; Kim, Changmu ; Lee, Hyang Bum ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 344~348
DOI : 10.4489/KJM.2014.42.4.344
A total of 118 fungal specimens were collected from Mt. Manggyeong, Chungnam province, Korea between July and October 2014. All specimens were identified to the species level using a combination of morphological characteristics and DNA sequencing. Out of 118 specimens, eight species, Amanita manginiana, A. pilosella, Calvatia holothurioides, Cantharellus tabernensis, Dicephalospora rufocornea, Gymnopus menehune, Marasmius brunneospermus, and Russula cerolens, have not been previously reported in Korea.
First Record of Endophytic Paraconiothyrium brasiliense Isolated from Chinese Maple Leaves in Korea
Paul, Narayan Chandra ; Lee, Hyang Burm ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 349~352
DOI : 10.4489/KJM.2014.42.4.349
The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internal transcribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense, a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomata formation was observed in the fungus cultured on potato dextrose agar at
for 7 days. Visible scattered black dots consisting of pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.
Characterization of Monilinia fructicola Associated with Brown Rot of Cherry Fruit in Korea
Choi, Hyo-Won ; Hong, Sung Kee ; Lee, Young Kee ; Nam, Young Ju ; Lee, Jae Guem ; Shim, Hong Sik ;
The Korean Journal of Mycology, volume 42, issue 4, 2014, Pages 353~356
DOI : 10.4489/KJM.2014.42.4.353
In August 2013, brown rot was observed on cherry fruits (Prunus avium L.) in Hwaseong city, Korea. Fruit rot first appears as small, circular brown spots that increase rapidly in size causing the entire fruit to rot. Grayish spores appear in tufts on rotted areas. Based on these morphological characteristics, the two isolates were identified as Monilinia fructicola. Molecular analysis of 5.8S subunit and flanking internal transcribed spacers (ITS1 and ITS2) was performed to confirm the identification. The ITS sequences had 100% identity with those of other reference M. fructicola isolates of NCBI GenBank. Pathogenicity was tested using spore suspension inoculation on wounded or unwounded cherry fruits. The lesions were observed on wounded and unwounded fruits after inoculation for 7 days. This is the first report on M. fructicola causing brown rot on cherry fruits in Korea.