Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Mycology
Journal Basic Information
Journal DOI :
The Korean Society of Mycology
Editor in Chief :
Volume & Issues
Volume 43, Issue 4 - Dec 2015
Volume 43, Issue 3 - Sep 2015
Volume 43, Issue 2 - Jun 2015
Volume 43, Issue 1 - Mar 2015
Selecting the target year
Nanoparticles as Alternative Pesticides: Concept, Manufacturing and Activities
Ahmed, Ahmed I.S. ; Lee, Youn Su ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 207~215
DOI : 10.4489/KJM.2015.43.4.207
Nanotechnology, which has become an important area of science, has caused an enormous developmental revolution in many fields. In the last two decades researchers have focused on overcoming the obstacles encountered during the preparation of nanoparticles. This article highlights the nanotechnology, along with a brief description of the manufacturing, concepts and activities of nanoparticles as alternative pesticides against plant pathogens, some methods for evaluation of nanoparticles against phytopathogens in vitro and in vivo, and explains the importance of some common nanoparticle types used in agricultural applications and plant pathology.
Notes on the Unrecorded Species of Subgenus Lepidella in Korean Genus Amanita
Jin, Yong Ju ; Yoo, Ki Bum ; Hong, Seung Beom ; Kwon, Soon Wu ; Kim, Soo Jin ; Kim, Seong Hwan ; Seok, Soon-Ja ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 216~223
DOI : 10.4489/KJM.2015.43.4.216
Amanita subgenus Lepidella is a well-known group that includes lethal amanitas. However, the taxonomic investigation of Korean Amanita species is still in progress. In this study, we collected Amanita specimens in Korea from 2009 to 2015 and classified 22 of them as subgenus Lepidella based on the morphology. Phylogenetic analysis of the 22 Amanita specimens based on the internal transcribed spacers sequences identified 16 phylogenetic species which included three undescribed Amanita species (A. sepiacea, A. modesta and A. kotohiraensis). Microscopic features of newly recorded Amanita species were photographed and line drawings were made. All collections cited here are deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences.
Notes on the New Species of Genus Mycena in Korea
Seok, Soon Ja ; Jin, Yong Joo ; Yoo, Ki Bum ; Hong, Seung Beom ; Kwon, Soon Wu ; Kim, Soo Jin ; Kim, Jeong Seon ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 224~230
DOI : 10.4489/KJM.2015.43.4.224
A re-study was conducted based on morphological characters of 39 specimens known as Mycena stylobates, collected from 33 areas, Mt. Chiack, Kangwon-do, etc. from 1988 to 2015 and deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences (HCCN). All specimens cited here were confirmed as a new to science, and designated as "Mycena yangsupensiae" which was described and illustrated in detail. Mycelial growth of four cultures from the fruiting bodies of Mycena sp. (KACC54181, KACC54182, KACC54183, and KACC54184) was high on the potato dextrose agar (PDA), at
. These four taxa were confirmed as the same species, Mycena yangsupensiae, by the internal transcribed spacer (ITS) sequencing analysis. All collections cited here are deposited in HCCN, Suwon.
Genome-wide Single Nucleotide Polymorphism-based Assay for Phylogenetic Relationship of the Flammulina velutipes
Woo, Sung-I ; Kim, Eun-Seon ; Han, Jae-Gu ; Jang, Kab Yeul ; Shin, Pyung-Gyun ; Oh, Youn-Lee ; Oh, Min Ji ; Jo, Sung-Hwan ; Lee, Jeong-Hee ; Kim, Kyung-Soo ; Kong, Won-Sik ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 231~238
DOI : 10.4489/KJM.2015.43.4.231
Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.
Analysis of Mycological Characteristics and Lignocellulose Degradation of Gyrodontium sacchari
Park, In-Cheol ; Seok, Soon-Ja ; Kim, Jeong-Seon ; Yoo, Jae-Hong ; Ahn, Jae-Hyung ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 239~246
DOI : 10.4489/KJM.2015.43.4.239
Two fungal strains were isolated from rods of Quercus sp. (NAAS02335) and Pinus densiflora (NAAS05299) in Korea. These strains were identified as Gyrodontium sacchari by their morphological and mycological characteristics. The optimal growth temperature of NAAS02335 and NAAS05299 are
, respectively. Production of cellulase, xylanase, and ligninase was tested on agar media supplemented dyes or substrates. Production of cellulase and xylanase of NAAS05299 was higher than those of NAAS02335, however ligninase activity of NAAS02335 was higher than that of NAAS05299. The activities of cellulase, xylanase, and amylase of strain NAAS05299 were estimated at 6.7~10.2 times higher than that of NAAS02335. Laccase activity was only estimated by strain NAAS02335. The lignocellulytic enzymes are induced by substrates such as rice straw, wooden chips of pine, oak, and poplar. The NAAS05299 was able to degrade filter paper completely after 4 weeks of culturing in liquid media containing a piece of filter paper at
with continuous shaking. NAAS05299 was able to degrade rice straw, pine chips, and oak chips after 4 months in solid culture, however NAAS02335 decomposed only rice straw among tested 4 kinds of biomass.
Study of Tannin Reducing Effect of Aronia by Yeast Isolated from Jeotgal
Shin, Hyo-Ju ; Byun, Ock-Hee ; Kim, Yu-Jin ; Bang, Bo-Yeon ; Park, Jung Min ; Jeong, Yong-Seob ; Bai, Dong-Hoon ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 247~252
DOI : 10.4489/KJM.2015.43.4.247
Aronia (Black chokeberry, Aronia melanocarpa) belonging to the Rosaceae family, is native to eastern North America. Aronia contain high levels of flavonoids, mostly anthocyanins and proanthocyanidins, which are known as condensed tannins. The dominant proanthocyanidins in aronia are (-)-epicatechin and (+)-catechin. The concentration of proanthocyanidins in aronia is higher than in other berries, however due to the astringent taste it is not desirable for consumption. Therefore, the purpose of this study is to evaluate the effect of aronia on the reduction in tannins by yeast isolated from regional Jeotgal. We isolated strains of yeast with high
-glucosidase activity from Jeotgal, with the MTY2 strains exhibiting a reduction in final tannin concentration according to thin layer chromatography (TLC) analysis. MTY2 was confirmed as Kazachstania servazzii using an 18S rDNA sequence and named as K. servazzii MTY2. K. servazzii MTY2 showed most significant growth when K. servazzii MTY2 was cultured in a solution of 10% (w/v) glucose, 3% (w/v) tryptone and 0.1% (w/v) sodium chloride. According to the high performance liquid chromatography (HPLC) analysis, the (+) - catechin peak is present, but (-) - epicatechin peak was reduced at culture condition added with 10% glucose in medium.
Control of Colletotrichum acutatum and Plant Growth Promotion of Pepper by Antagonistic Microorganisms
Han, Joon-Hee ; Kim, Moon-Jong ; Kim, Kyoung Su ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 253~259
DOI : 10.4489/KJM.2015.43.4.253
Anthracnose caused by Collectotrichum acutatum is the most devastating disease of pepper plants in Korea. In this study, we evaluated the effect of selected antagonistic bacteria on control of anthracnose and plant growth promotion of pepper plants under field conditions. Four different bacterial isolates used in the current study were isolated from the pepper rhizosphere (GJ01, GJ11) and tidal flat (LB01, LB14) in previous studies. Four bacterial isolates, together with a control strain (EXTN-1), showed antifungal activity against C. acutatum in a dual culture assay. To test for plant growth promotion effect, seedling vigor index and growth parameters of pepper were measured under field condition. As a result, all four bacterial isolates were effective for improving plant growth promotion. The strain GJ01 was the most effective in improving the seedling vigor on pepper, but the strain GJ11 in increasing the pepper fruit yield. The incidence of anthracnose was inhibited in the range of 63.2~72.5% by treatment of four bacterial isolates. The current study indicated that the four bacterial isolates could be used as potential biological control agents of anthracnose disease of pepper.
Baseline Sensitivity and Monitoring for the Resistance to Benomyl of Fusarium Species Causing Bakanae Disease in Korea
Choi, Hyo-Won ; Lee, Yong Hwan ; Hong, Sung Kee ; Lee, Young Kee ; Lee, Jae Guem ; Kim, Hyo Won ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 260~266
DOI : 10.4489/KJM.2015.43.4.260
To examine the changes in resistance to benomyl of Fusarium species causing bakanae disease, Fusarium isolates were collected in Korea, and pathogenicity tests were performed using rice seeds in vitro. Minimum inhibitory concentration (MIC) and effective concentration of 50% (
) values of isolates were examined using the agar dilution method. High frequency distribution of MIC values to benomyl against isolates collected in 2006~2007 and 2013~2014 years were
and more than
, respectively. The mean
value of isolates to benomyl increased from
in 2006~2007 to
in 2013~2014. Based on MIC and
values of isolates, the moderate resistance of benomyl were determined as more than
of MIC and less
value, and resistant isolates to benomyl were determined as more than
value. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistance isolates in 2013~2014 increased from 12.5% to 36.4%. In addition, multiple resistant isolates to prochloraz as well as benomyl increased to 20.3% in 2013~2014.
Isolation and Identification of Yeasts from Jeju Island Soils
Han, Sang-Min ; Bae, Sang-Min ; Han, Jae-Won ; Kim, Ji-Yoon ; Lee, Jong-Soo ; Kim, Ha-Kun ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 267~271
DOI : 10.4489/KJM.2015.43.4.267
Significant differences in annual precipitates on Jeju island have been reported depending on the location. We collected soil samples from east and west areas of Jeju Island to identify yeasts by plating on yeast peptone dextrose plates and subsequent analysis for the polymerase chain reaction amplified D1/D2 region of 26S rDNA of colonies. As a result, 20 yeast strains of 12 species were isolated from 7 different sampling sites in east area and 13 yeast strains of 6 species from 5 different sampling sites in west area. Some differences in yeast flora were observed depending on the sample collection sites having different annual precipitates.
First Report of Three Species of Endophytic Fungi Isolated from Needle Leaves of Conifers in Korea
Eo, Ju-Kyeong ; Lee, Bong-Hyung ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 272~276
DOI : 10.4489/KJM.2015.43.4.272
We examined endophytic fungi from the needle leaves of three species of conifers in Korea: Abies nephrolepis, Thuja koraiensis, Pinus koraiensis. Endophytic fungi were isolated from the surface-sterilized leaves and identified based on morphological characteristics and Internal transcribed spacer sequences of rDNA. Three species of endophytic fungi isolated in this study were the first reported in Korea: Cryptosporiopsis actinidiae, Pestalotiopsis mangiferae, Pyrenochaeta cava.
First Report of Gray Mold Disease of Sweet Basil (Ocimum basilicum) Caused by Botrytis cinerea in Korea
Aktaruzzaman, Md. ; Kim, Joon-Young ; Afroz, Tania ; Hong, Sae-Jin ; Kim, Byung-Sup ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 277~280
DOI : 10.4489/KJM.2015.43.4.277
In August 2015, we collected samples of gray mold from sweet basil growing in Sachunmeon, Gangneung, Gangwon Province, Korea. Symptoms included extensive growth of mycelia with gray conidia on young leaves, stems, and blossoms. The pathogen was isolated from infected leaves and blossoms and the fungus was cultured on potato dextrose agar. For identification of the fungus, morphology and rDNA sequencing analysis of the fungus were performed, which confirmed its pathogenicity according to Koch's postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4) and the three nuclear protein-coding genes G3PDH, HSP60, and RPB2 showed that the causal agent was Botrytis cinerea. This is the first report of gray mold caused by Botrytis cinerea on sweet basil in Korea.
Effect of Lime Sulfur on Changes of Fungal Diversity in Pear Fallen Leaves
Min, Kwang-Hyun ; Song, Jang Hoon ; Cho, Baik Ho ; Yang, Kwang-Yeol ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 281~285
DOI : 10.4489/KJM.2015.43.4.281
This study was conducted to examine changes in the fungal community on fallen leaves of pear by treatment with lime sulfur. Although the lime sulfur could reduce the primary inoculum of several pathogens on spring season, the effect of lime sulfur has not been well determined scientifically. Fallen leaves infected by pear diseases in pear orchards in Naju were collected and treated with lime sulfur or water as a control. To determine the fungal diversity from each treatment, rDNA internal transcribed spacer (ITS) regions were analyzed after extraction of fungal genomic DNA from lime sulfur-treated or water-treated fallen leaves, respectively. The most common fungal species were Ascomycota and Basidiomycota in both treated leaves. However, the population dynamics of several fungal species including Alternari sp., Cladosporium sp., and Phomopsis sp., which are known as pear pathogens for skin sooty dapple disease, were quite different from each treated leaves. These results indicated that lime sulfur treatment led to changes of fungal communities on pear fallen leaves and could be applicable as a dormant spray.
Optimal Storage Condition of Clubroot Pathogen, Plasmodiophora brassicae for Artificial Inoculation
Yang, Seul Gi ; Park, Ju Young ; Seo, Mun Won ; Kim, Hong Gi ;
The Korean Journal of Mycology, volume 43, issue 4, 2015, Pages 286~289
DOI : 10.4489/KJM.2015.43.4.286
Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is a severe soilborne disease of Brassicaceae. Storage of clubroot gall is important for studies on pathogenicity and race identification. As the current storage method has been used for more than 100 years, a new storage method should be developed and the most efficient way maintaining pathogenicity should be determined. Effects of storage conditions with different storage periods on pathogenicity in galls of kimchi cabbage were examined in a greenhouse. The experiments were performed under six conditions and four temperatures in order to determine the most effective storage conditions for maintenance of pathogenicity. The most effective conditions for clubroot gall storage was the storage of whole gall at
or storage of filtrate at the same temperature through eight layers of gauze after homogenization of the galls.