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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Mycology
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Journal DOI :
The Korean Society of Mycology
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Volume & Issues
Volume 44, Issue 2 - Jun 2016
Volume 44, Issue 1 - Mar 2016
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Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea
Han, Sang-Min ; Han, Jae-Won ; Bae, Sang-Min ; Park, Won-Jong ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 1~7
DOI : 10.4489/KJM.2016.44.1.1
This study focused on isolation and identification of wild yeasts from soils in fields near mountains and elucidation of its yeast distribution. Several kinds of yeasts were isolated from various soils of Daejeon metropolitan city and Chungcheongnam-do in Korea and identified by BLAST search of nucleotide sequences of internal transcribed spacer (ITS) region including 5.8S rRNA and D1/D2 region of 26S rDNA. Ninety-seven strains of 20 species from 61 soil samples were isolated, of which Cryptococcus podzolicus (11 strains), Debaryomyces hansenii (6 strains), and Trichosporon asahii (6 strains) were dominant species.
Unrecorded Fungal Species Isolated from Greenhouses Used for Shiitake Cultivation in Korea
Ahn, Geum Ran ; Kwon, Hyuk Woo ; Ko, Han Kyu ; Kim, Seong Hwan ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 8~15
DOI : 10.4489/KJM.2016.44.1.8
Fungal contamination is a detrimental factor affecting sawdust media-based shiitake cultivation in greenhouses. During fungal monitoring of greenhouses used for shiitake cultivation, eight fungal species were isolated and identified from indoor air and mushroom flies collected in the greenhouses. The current study reported five species as new in Korea, viz. Ascochyta hordei, Discosia artocreas, Mucor nidicola, Perenniporia medulla-panis, and Pseudozyma prolifica, and confirmed two species, Penicillium charlesii and Penicillium brevicompactum, which were previously recorded in Korea without molecular taxonomic validation. The morphological characteristics and phylogenetic relationships based on nucleotide sequences of the internal transcribed spacer rDNA region or calmodulin gene were described for all identified species.
Community Structure of Arbuscular Mycorrhizal Fungi in the Islands of Chungnam, Korea
Lee, Jeong-Youn ; Lee, Eun-Hwa ; Ka, Kang-Hyeon ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 16~22
DOI : 10.4489/KJM.2016.44.1.16
Five islands (Sinjindo, Mado, Daenanjido, Wonsando, and Sapsido) and the coastal area (Muchangpo) in Chungnam, Korea, were selected to determine the diversity of arbuscular mycorrhizal (AM) fungi. Soil-inhabiting AM fungi were isolated and identified on the basis of morphological characteristics and sequence analyses of 18s rDNA. The differences in the fungal community structures were compared among sites. As a result, 24 species of AM fungi were identified, of which two species of AM fungi, Acaulospora brasiliensis and Redeckera fulvum, were isolated for the first time in Korea. This study revealed that AM fungal spore abundance was low and the genus Acaulospora was dominant in most of the islands. AM fungal community structures in five Islands were highly similar. However, the coastal area, Muchangpo, had different AM fungal community structure from the islands.
Comparison of Ingredients and Antioxidant Activity of the Domestic Regional Wolfiporia extensa
Choi, Su-Hee ; Lee, Seung-Jin ; Jo, Woo-Sik ; Choi, Jong-Woon ; Park, Seung-Chun ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 23~30
DOI : 10.4489/KJM.2016.44.1.23
This study was conducted for comparison of ingredients, phytochemical compounds and antioxidant activity of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, and Jeolla-do. Three contents of Wofiporia extensa were analyzed as oxygen (46~48%), carbon (38~39%), hydrogen (6.05~6.1%) and nitrogen (0.17~0.21%). The mineral contents of 50% ethanol Wofiporia extensa extracts were measured as sulfur (S) 145~149 ppm, Magnesium (Mg) 69~72 ppm, phosphorus (P) 122~154 ppm and calcium (Ca) 210.61~509.98 ppm. Wofiporia extensa from Gyeongsang-do (509.98 ppm) contained a significantly higher quantity of Ca than that from Gangwon-do (210.62 ppm) and Jeolla-do (223.88 ppm). In the gas chromatograph-mass spectrometry (GC-MS) analysis, oleic acid was identified in three 50% ethanol Wofiporia extensa extracts. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2`-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay for antioxidant activity, the
values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do and Jeolla-do were calculated as 2.966 mg/mL, 23.03 mg/mL, and 4.16 mg/mL and 3.521 mg/mL, 12.17 mg/mL, and 7.40 mg/mL. In the ferric reducing antioxidant power (FRAP) assay, the
values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, Jeolla-do were 6.585 mg/mL, 19.06 mg/mL, and 18.97 mg/mL, respectively. In summary, Wofiporia extensa cultured in Gangwon-do had stronger antioxidant activity and higher concentration of oleic acid than that of Geyongsang-do and Jeolla-do. However, Wofiporia extensa cultured in Geyongsang-do contained a much higher concentration of Ca than that of Gangwon-do and Jeolla-do.
Recycle Effect of Post-harvest Medium of King Oyster Mushroom (Pleurotus eryngii)
Cheong, Jong-Chun ; Lee, Chan-Jung ; Moon, Ji-Won ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 31~35
DOI : 10.4489/KJM.2016.44.1.31
This study was conducted to test whether post-harvest medium of king oyster mushroom can be recycled for its bottle cultivation. Physical properties of the post-harvest medium and the resulting yield of fruit bodies revealed no significant difference compared with control. Recycling post-harvest medium can be replaced to 15% of the basal medium, thus decreasing the cost of raw materials up to 15%.
Evaluation of Rhizobacterial Isolates for Their Antagonistic Effects against Various Phytopathogenic Fungi
Kim, Yun Seok ; Kim, Sang woo ; Lamsal, Kabir ; Lee, Youn Su ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 36~47
DOI : 10.4489/KJM.2016.44.1.36
This study was conducted to evaluate five different strains of rhizobacterial isolates viz. PA1, PA2, PA4, PA5 and PA12 for biological control against Colletotrichum acutatum, C. coccodes, C. gloeosporioides, C. dematium, Botrytis cinerea, Rhizoctonia solani, Sclerotinia minor and Fusarium sp. In vitro inhibition assay was performed on three different growth mediums, potato dextrose agar (PDA), tryptic soy agar (TSA), and PDA-TSA (1:1 v/v) for the selection of potential antagonistic isolates. According to the result, isolate PA2 showed the highest inhibitory effect with 65.5% against C. coccodes on PDA and with 96.5% against S. minor on TSA. However, the same isolate showed the highest inhibition with 58.5% against C. acutatum on PDA-TSA. In addition, an in vivo experiment was performed to evaluate these bacterial isolates for biological control against fungal pathogens. Plants treated with bacteria were analyzed with phytopathogens and plants inoculated with phytopathogens were treated with isolates to determine the biological control effect against fungi. According to the result, all five isolates tested showed inhibitory effects against phytopathogens at various levels. Mode of action of these rhizobacterial isolates was evaluated with siderophore production, protease assay, chitinase assay and phosphate solubilizing assay. Bacterial isolates were identified by 16S rDNA sequencing, which showed that isolates PA1 and PA2 belong to Bacillus subtilis, whereas, PA4, PA5, and PA12 were identified as Bacilus altitudinis, Paenibacillus polymyxa and Bacillus amyloliquefaciens, respectively. Results of the current study suggest that rhizobacterial isolates can be used for the plant growth promoting rhizobacteria (PGPR) effect as well as for biological control of various phytopathogens.
First Report of Metacordyceps chlamydosporia (Cordyceps chlamydosporia) Isolated from Soil in Korea
Kim, Hyun Seung ; Adhikari, Mahesh ; Yadav, Dil Raj ; Kim, Sang Woo ; Um, Yong Hyun ; Lee, Hyang Burm ; Lee, Youn Su ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 48~50
DOI : 10.4489/KJM.2016.44.1.48
A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in Korea. Identification of the fungal species was based on morphological and molecular characteristics. This species has not been previously reported in Korea and herein we present data with detailed descriptions and figures.
Notes on Five Endophytic Fungal Species Isolated from Needle Leaves of Conifers in Korea
Lee, Bong-Hyung ; Eo, Ju-Kyeong ; Eom, Ahn-Heum ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 51~56
DOI : 10.4489/KJM.2016.44.1.51
Diverse endophytic fungi were isolated from surface-sterilized leaves of three species of conifers inhabiting various sites in Korea: Abies nephrolepis, Pinus koraiensis and Taxus cuspidate. The isolates were identified based on morphological characteristics and sequences analysis of both internal transcribed spacer and large subunit regions of rDNA. In this paper, we report on five previously unreported species of endophytic fungi isolated from conifers: Biscogniauxia maritime, Nemania diffusa, Pezicula carpinea, Phomopsis juglandina and Sydowia polyspora.
Antihyperglycemic Effect of Water Extracts from Pleurotus cornucopiae-Containing α-Glucosidase Inhibitor
Shin, Ja-Won ; Bae, Sang-Min ; Han, Sang-Min ; Lee, Yun-Hae ; Jeong, Youn-Kyung ; Ji, Jeong-Hyun ; Lee, Jong-Soo ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 57~60
DOI : 10.4489/KJM.2016.44.1.57
Following preparation of water extracts of Pleurotus cornucopiae fruiting body-containing
-glucosidase inhibitor, their antihyperglycemic effects were examined using streptozotocin-induced diabetic Sprague-Dawley (SD)-rats. The water extracts from Pleurotus cornucopiae showed dosage-dependant antihyperglycemic effects on the streptozotocin-induced diabetic SD-rats after oral administration to 120 min on the short time test and 4 days on the long time test, respectively. The water extracts from Pleurotus cornucopiae fruiting body showed dosage-dependent hypoglycemic action after administration to 120 min and 4 days in the SD-rat and streptozotocin-induced diabetic SD-rat.
Sclerotium Rot of Cowpea (Vigna sinensis King) Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Kang, Dong-Wan ; Han, Inyoung ; Choi, Yong-Jo ; Lee, Sang-Dae ; Son, Daeyoung ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 61~63
DOI : 10.4489/KJM.2016.44.1.61
Sclerotium rot disease on cowpea (Vigna sinensis King) was observed in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services in September 2015. Lesions were covered by white mycelial mats, and numerous sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1~3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on potato dextrose agar (PDA) was
, with the hyphal width of
. For molecular identification, the complete internal transcribed spacer (ITS) rDNA region of the causal fungus was sequenced and analyzed. Based on the mycological characteristics, ITS rDNA sequence analysis, and pathogenicity test, this fungus was identified as Sclerotium rolfsii. This is the first report of sclerotium rot on cowpea caused by S. rolfsii in Korea.
First Report of Ampelomyces quisqualis from Sycamore and Crape Myrtle and Its Potential as a Mycoparasite of Powdery Mildew
Nguyen, Thi Thuong Thuong ; Lee, Hyang Burm ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 64~67
DOI : 10.4489/KJM.2016.44.1.64
During screening fungi as potential biological control agents for plant parasitic fungi, a fungal strain, EML-FAM3, was isolated from powdery mildew leaf lesions caused by Erysiphe platani on sycamore (Platanus occidentalis L.), and another strain, EML-FAMC1, from Erysiphe australiana on crape myrtle (Lagerstroemia indica L.). Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacers (ITS1 and ITS2) and 5.8S rDNA, the strains were identified as Ampelomyces quisqualis. To our knowledge, this is the first report of new mycohosts, E. platani and E. australiana, of the mycoparasite A. quisqualis on sycamore and crape myrtle plants. The hyperparasite may represent the potential for controlling E. platani and E. australiana epidemics.
First Report of Post-Harvest Fruit Rot of Aronia melanocarpa Caused by Fusarium tricinctum in Korea
Lee, Hye Won ; Nguyen, Thi Thuong Thuong ; Lee, Hyang Burm ;
The Korean Journal of Mycology, volume 44, issue 1, 2016, Pages 68~71
DOI : 10.4489/KJM.2016.44.1.68
Black chokeberry, Aronia melanocarpa (Michx.) Elliott, is commonly used as a source of jam and jelly in Korea and worldwide. A fungal isolate EML-CCB6 was isolated from the decaying fruit of black chokeberry. Based on the morphological characteristics and rDNA internal transcribed spacer sequence analysis, the isolate was identified as Fusarium tricinctum (Corda) Sacc. This is the first report of post-harvest fruit rot of black chokeberry caused by F. tricinctum in Korea.