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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Malacology
Journal Basic Information
Journal DOI :
The Malacological Society of Korea
Editor in Chief :
Volume & Issues
Volume 24, Issue 3 - Dec 2008
Volume 24, Issue 2 - Aug 2008
Volume 24, Issue 1 - May 2008
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Spermatogenesis and Reproductive Cycle in Male Spisula sachalinensis (Bivalvia: Mactridae) of Korea
Lee, Ki-Yong ; Chung, Ee-Yung ; Lee, Jeong-Yong ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 1~10
Spermatogenesis and the reproductive cycle in male Spisula sachalinensis were investigated by cytological and histological observations. The morphology of the spermatozoon has a primitive type and is similar to those of other bivalves in that it contains a short midpiece with four mitochondria surrounding the centrioles. But spermatozoon of this species has not axial rod and satellite body in the midpiece. The morphologies of the sperm nucleus type and the acrosome shape of this species have a globe-shape type and modified cap-like shape, respectively. The spermatozoon is approximately
in length including the sperm nucleus length (about
), acrosome length (about
) and tail flagellum. The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. The spawning period of these species lasts from June to July, and the main spawning occurs in July when seawater temperatures are greater than
. The male reproductive cycle of this species can be categorized into five successive stages: early active stage (October to January), late active stage (February to April), ripe stage (April to June), partially spawned stage (June and July), and spent/inactive stage (August to September).
Geographic Variations between Jedo Venus Clam (Protothaca jedoensis, Lischke) Populations from Boryeong and Wonsan of Korea
Park, Gi-Sik ; Yoon, Jong-Man ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 11~24
GDNA was isolated from the jedo venus clam (Protothaca jedoensis, Lischke) from Boryeong (jedo venus clam from Boryeong JVCB) and Wonsan (jedo venus clam from Wonsan; JVCW) located in the West Sea and the East Sea of Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the populations genetic variations. In the present study, the seven decamer primer generated the one hundred and eleven major/minor specific bands in JVCB population and ninety four-specific bands in JVCW population. Seven primers generated the unique shared bands to each population of one hundred and seventy-six, on average of 25,1, in JVCB population from Boryeong and three hundred thirty, on average of 47,1, in JVCW population from Wonsan, respectively. The dendrogram obtained by the seven oligonucleotides primers, indicates two genetic clusters. Especially, two Protothaca between the individual WONSAN no. 12 and BORYEONG no. 10 showed the longest genetic distance (0.537) in comparison with other individuals used. Accordingly, RAPD analysis showed that the JVCB was a little more genetically diverse than the JVCW population. This result implies the genetic similarity owing to rearing in the same and/or similar circumstances or inbreeding within the JVCW population. So to speak, JVCB population may have high levels of genomic DNA variability owing to the introduction of the wild individuals from the other sites to sampling sites although it may be the geographically diverse distribution of this species. However, it was confirmed that it did not appear like that really in this study. We feel convinced that RAPD analysis discovered a significant genetic distance between two Protothaca population pairs (P<0.001). The existence of population discrimination and genetic diversity between two Protothaca populations was identified by RAPD analysis.
Bacteriological Characteristic of Atrina pectinata and Ruditapes philippinarum under Non-refrigerated and Refrigerated Storage Conditions
Kang, Kyoung-Ho ; Kim, Byeong-Hak ; Kim, Young-Hun ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 25~32
In order to estimate the necessity of refrigerated storage of fresh seafood for short-term storage, and evaluate the effect of refrigerated storage on pen shell Atrina pectinata and clam Ruditapes philippinarum collected from Jang-su of Deukryang Bay and I-mok of Sunchen Bay in South Korea, the counts of coliform, Escherichia coli and total aerobic bacteria in A. pectinata and R. philippinarum under non-refrigerated
and refrigerated storage conditions
were determined. The results indicated that the storage at temperature of
possessed significant effects on inhibiting bacterial growth in live seafood. And refrigerated storage had different effect on A. pectinata and R. philippinarum. Different species and culture environments significantly influenced the initial and ultima bacteria counts. This study confirmed that refrigerated storage for short-term storage of live seafood was necessary, and indicated that the effect of refrigerated storage was influenced by comprehensive effectors.
Ultrastructural Study of the Process of Oocyte Degeneration and Function of the Follicle Cells in Female Spisula sachalinensis on the East Sea of Korea
Chung, Ee-Yung ; Lee, Ki-Young ; Lee, Jeong-Yong ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 33~40
Ultastructural studies of oocyte degeneration and follicle cells in female Spisula sachalinensis are described for clams collected from Jumunjin, Gangwondo, Korea. The follicle cells playan integral role in vitellogenesis and oocyte degeneration by assimilating products originating from the degenerated oocytes (thus allowed the transfer of yolk precursors needed for vitellogenesis). The functions of the follicle cells include phagocytosis and intracellular digestion of products originating from oocyte degeneration. During the period of oocyte degeneration, follicle cells of this species probably have lysosomal systems for the breakdown and reabsorption of various phagosomes(phagolysosomes) in the cytoplasm for nutrient storage; this process has been observed in other bivalves.
Mantle Ultrastructure of the Spiny Top Shell, Batillus cornutus (Gastropoda: Turbinidae)
Jung, Gui-Kwon ; Park, Jung-Jun ; Jin, Young-Guk ; Ju, Sun-Mi ; Lee, Jae-Woo ; Jung, Ae-Jin ; Lee, Jung-Sick ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 41~50
The histochemical characteristics and ultrastructure of the mantle in the spiny top shell, Batillus cornutus were described using light and electron microscopy. The simple epidermal layer wrapped on the top and bottom of the centrally located connective tissue. And then the epidermal layer were divided into the outer epidermal layer near a shell and the inner epidermal layer closed to the visceral mass. The connective tissue layer was composed of the collagen fiber muscularfiber bundle and hemolymph sinus. Mucous cells in the apical mantle contained acid and neutral mucopolysaccaride, and acidic carboxylated mucopolysaccaride in the mid and marginal mantle. The mantle thickness, epidermal layer thickness and hemolymph sinus area displayed a trend of reduction from the marginal zone to the apical zone. From TEM observation, it was possible to distinguish epithelium, ciliated cell, absorptive cell and secretory cell in the epidermal layer. The epithelia were columnar and the nucleus was elliptical. The free surface were covered with microvilli. The lateral membranes of epithelium was con nected with neighboring cells by the zonular occludens, zonular adherens and membrane interdigitation. Ciliated cell on free surface had cilia and microvilli, and numerous mitochondria in the apical cytoplasm. In the epidermal layer, it observed 2 type cells having absorptive function. The absorptive cells were columnar in shape, and contained microvilli, pinocytotic vesicles, mitochondria and lysosomes of various electron density. Secretory cells can be divided into four types (A, B, C, D) depending on the cell shape and characteristics of secretory granules. These cells were unicellular glands and had similar characteristics to previously reported on the mantle of the gastropod and bivalves.
Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm
Kang, Kyoung-Ho ; Seon, Seung-Chun ; Zhou, Bin ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 51~57
With the purpose to estimate the possibility of short-term storage and cryopreservation for sperm of Charonia sauliae, which is a potential preparation for its artificial reproduction and further research, in this study, protocols for short-term storage and cryopreservation of trumpet shell sperm was optimized. The effects of different immobilizing solutions, dilution ratios were estimated for short-term storage. And the effects of different cryoprotectant extenders and freezing rates were estimated for cryopreservation in terms of motility and survival of sperm. The results indicated that the artificial sea water of 350 mOsmol/kg is a better immobilizing solution and sperm which was diluted at a ratio of 1:1 (v/v) had higher motility and survival rate during short-term storage. The effect of 5% dimethyl sulfoxide was significantly better than those of other cryoprotectant extenders. And a freezing rate of
showed better effect than other freezing rates. In conclusion, this study optimized some key factors of the short-term and cryopreservation of C. sauliae sperm, which can provide valuable data for germ-plasm conservation and artificial propagation of C. sauliae.
Influence of Temperature, Salinity and Hypoxia on Survival and Metabolic Rate in the Ark Shell, Scapharca broughtonii
Shin, Yun-Kyung ; Kim, Byoung-Hak ; Choi, Nack-Joong ; Jung, Choon-Goo ; Park, Min-Woo ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 59~66
The ark shell, Scapharca broughtonii were collected from the Jinhae bay in Kyungnam, Korea from April 2006 to October 2007. To assess how much they have tolerance against hypoxia under the compound condition of temperature and salinity, survivorship, oxygen consumption rate and external features of Scapharca broughtonii were measured, The 7 days-
of dissolved oxygen (DO) for Scapharca broughtonii at
-ordinary sea water and
psu was 1.98mg/L (confidence limit: 1,45-2.39 mg/L) and 2.63mg/L (confidence limit: 1.76-3.37mg/L) respectively. Whereas 3 days-
-ordinary sea water was 3.89mg/L (confidence limit: 3.36-4.55 mg/L) and 4 days-
psu was 3.74mg/L (confidence limit: 3.19-4,43 mg/L). Oxygen consumption rate with each experimental group was decreased during the period exposed to hypoxia. To recovery, they were replaced to ordinary and oxygen consumption rate was increased a little, but all died, The color of the umbo of shell in Scapharca broughtonii was changed dark during hypoxia. These data will provide important fundamental information for examining the causes of mass mortality of shellfish in the summer.
Proximate Composition in the Muscle and Viscera of Five Veneridae Clams (Bivalvia) from Southern Coast of Korea
Yoon, Ho-Seop ; An, Yun-Keun ; Choi, Sang-Duk ; Kim, Jung ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 67~72
This study investigated the proximate composition in the muscle and viscera of five commercially valuable Korean Veneridae, Protothaca jedoensis, Ruditapes philippinarum, Saxidomus purprratus, Cyclina sinensis and Meretrix lusoria. The proximate analysis revealed that the moisture, crude protein and carbohydrate of muscle in higher than viscera. As results, except for the crude lipid and carbohydrate there was little significant differences among the moisture, protein and crude ash. The crude lipid contents was highest in the viscera of Meretrix lusoria, while lowest in the muscle of Ruditapes philippinarum. The carbohydrate content of viscera in the Meretrix lusoria, Ruditapes philippinarum and Protothaca jedoensis were
Molecular Phylogenetic Study of Nesiohelix samarangae Based on Metallothionein Gene
Lee, Jun-Seo ; Min, Byung-Jun ; Kang, Se-Won ; Lee, Jae-Bong ; Baek, Moon-Ki ; Hwang, Seung-Young ; Kim, So-Hee ; Kho, Weong-Gyu ; Choi, Sang-Haeng ; Chae, Sung-Hwa ; Park, Hong-Seog ; Han, Yeon-Soo ; Lee, Jun-Sang ; Jeong, Kye-Heon ; Lee, Yong-Seok ;
The Korean Journal of Malacology, volume 24, issue 1, 2008, Pages 73~80
Numerous morphological studies on N. samarangae have been well conducted over the last ten years. In this context, we have attemtped to do molecular phylogenetic analysis by using metallothionein (MT) gene from N. samarangae. To this end, we cloned the full length cDNA of MT from cDNA library of N. samarangae. The complete cDNA sequences were obtained from the expressed sequence tag (EST) sequencing project of N. samarangae, The coding region of 195 bp gives an amino acid sequence of 65 residues including methionine. There are 5' (61 bp) and 3' (48 bp) untranslated region at both ends of the Ns-MT cDNA sequence. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Ns-MT cDNA indicate that N. samarangae has similarity to land snails such as Helix pomatia, Helix aspersa and Arianta arbustorum.