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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Malacology
Journal Basic Information
Journal DOI :
The Malacological Society of Korea
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Volume & Issues
Volume 29, Issue 4 - Dec 2013
Volume 29, Issue 3 - Sep 2013
Volume 29, Issue 2 - Jun 2013
Volume 29, Issue 1 - Mar 2013
Selecting the target year
Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum
Park, Kyung-Il ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 1~6
DOI : 10.9710/kjm.2013.29.1.1
This study aimed to evaluate the effects of shaking stress in the hemolymph of the Manila clam Ruditapes philippinarum by quantification of nitric oxide (NO) levels. The clams were divided into 3 groups as follows: clams placed in a plain container (control), clams injected with nitro-L-arginine methyl ester (NAME, an NO inhibitor), and clams in a container filled with nylon fiber at a density of
. Subsequently, each group was placed in sea water and shaken at 100 rpm for 6 h. The concentration of NO was quantified by using DAF assay and Griess assay. Both the assays showed that while shaking significantly increased the NO concentration, the NO inhibitor reduced the NO concentration in the hemolymph of the clams tested. In addition, the nylon fiber, which was used as a filler, effectively prevented the increase in NO concentration. This result suggests that measurement of NO concentration is a useful tool for evaluation of physiological stress in marine bivalves. In addition, it should be considered that a filler is necessary when dredge fishing or the suspended clam culture method is developed.
Changes of Biomarker in Manila clam, Ruditapes philippinarum Exposed to Lead
Shin, Yun Kyung ; Park, Jung Jun ; Lim, Hyun Sig ; Lee, Jung Sick ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 7~13
DOI : 10.9710/kjm.2013.29.1.7
This study was conducted to find out biological response of Manila clam, Ruditapes philippinarum exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.25, 0.50 and 1.00 mg/l). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and histopathology of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 25%-72%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of lead increased the accumulation of lipofuscin increased.
Quantification of nitric oxide concentration in the hemocytes of Manila clam Ruditapes philippinarum by using 4,5-diaminofluorescein diacetate (DAF-2) detection method
Nam, Ki-Woong ; Yang, Hyun-Sung ; Park, Kyung-Il ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 15~21
DOI : 10.9710/kjm.2013.29.1.15
Nitric oxide (NO) is an important intra-intercellular signaling molecule that regulates many physiological processes and participates in the development some pathological conditions in animals. In this study, we compared different methods for determining NO concentration in the hemocytes of Manila clam Ruditapes philippinarum. For measuring the intracellular NO levels, we used the specific fluorescent probe 4,5-diaminofluorescein diacetate (DAF-2 DA), and the quantification methods that were compared were based on image analysis, spectrophotometry, and flow cytometry. NO concentration could be determined using all the 3 methods, and the concentration varied significantly depending upon the presence of NO regulators in the hemocytes; NO concentration increased in the presence of L-arginine, while it decreased in the presence of N-nitro-L-arginine methyl ester. In particular, it is found that estimation of NO using a flowcytometry is more economical, reliable and accurate compared to image analysis and spectrophotometry. Accordingly we believe that determining NO concentration by using flowcytometry will be useful in evaluating physiological and pathological conditions in marine bivalves.
Effects of ultraviolet radiation on the toxicity of water-accommodated fraction and chemically enhanced water-accommodated fraction of Hebei Spirit crude oil to the embryonic development of the Manila clam, Ruditapes philippinarum
Lee, Chang-Hoon ; Sung, Chan-Gyoung ; Kang, Sin-Kil ; Moon, Seong-Dae ; Lee, Ji-Hye ; Lee, Jong-Hyeon ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 23~32
DOI : 10.9710/kjm.2013.29.1.23
The purpose of this study is to evaluate the effects of oil dispersant and ultraviolet (UV) radiation on the toxicity of crude oil. The toxicity of water-accommodated fraction (WAF) and chemically enhanced water-accommodated fraction (CEWAF) of Hebei Spirit crude oil was investigated in the embryo of the Manila clam, Ruditapes philippinarum with- and without ultraviolet radiation. The WAF and CEWAF with- and without UV radiation affected significantly the embryonic development of R. philippinarum. The EC50s of WAF without UV, WAF with UV, CEWAF without UV, and CEWAF with UV were 2.82, 0.79, 1.60, and 0.45 g/L, respectively. CEWAF was 1.6 times more toxic than WAF. UV radiation increased crude oil toxicity to 3.6 times for both WAF and CEWAF. The oil dispersant and UV radiation did not affect the acute toxicity to the embryo but retarded the period of embryonic development up to 26%. R. philippinarum proved to be a sensitive species to reflect the toxic effects of oil spill combined with oil dispersant and UV radiation. It is suggested that the chemical analyses on the WAF and CEWAF is important for the identification and quantitative explanation of the phototoxic compounds in crude oil.
Concentration of Heavy Metal and Alteration of Reproductive and Histological Biomarker of Mytilus galloprovincialis in Gamak Bay of the Southern Coast of Korea
Jeon, Mi Ae ; Kang, Ju Chan ; Lee, Jung Sick ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 33~41
DOI : 10.9710/kjm.2013.29.1.33
This study investigated concentration of metal ions, intersexuality and histological alterations of digestive gland in Mytilus galloprovincialis. Samples were collected from two areas of Gamak Bay in May, 2010. The concentration of metal ions showed that Al (
mg/kg) was highest and Zn (
mg/kg) was second high concentration. Co (
mg/kg) was lowest. The intersexuality was 26.4% and males (38.8%) was higher than the females (12.9%). Intersex type was observed four types. Destruction of digestive tubule epithelium was highest among other biomarker in digestive gland. Distribution of basophilic cell and lipofuscin was 6.1% and 1.5%, respectively.
Study on Optimal Condition for Oyster Rack Culture in terms of tidal exposure and rack height in Wando Coast, Korea
Han, Hyon Sob ; Cho, Sang-Man ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 43~50
DOI : 10.9710/kjm.2013.29.1.43
We investigated the growth performance of oysters (initial shell height
mm) under differing conditions of tidal exposure time and culture rack height in an experiment that commenced in April, 2011. Significant differences were observed in shell height from June 2011, in total weight from August, and in meat weight from September. Fatness tended to decrease during the experimental period, but was not significantly different at the end of the experiment. Significant differences in survival rates were mainly observed from June to August. After September, further changes were not observed in any experimental treatment group. The greatest growth potential (
) and survival rate were observed at a sea level of approximately 116 cm. The results indicate that in the study area the use of oyster culture conditions involving 1 or 2 h of tidal exposure and 60 - 70 cm rack height could result in oysters reaching the favored commercial half shell size within 14 months, with > 80% survival.
The Geochemical Characteristics and Environmental Factors on the Marine Shellfish Farm in Namhae-po Tidal Flat of Taean
Choi, Yoon Seok ; Park, Kwang Jae ; Yoon, Sang Pil ; Chung, Sang Ok ; An, Kyoung Ho ; Song, Jae Hee ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 51~63
DOI : 10.9710/kjm.2013.29.1.51
To assess the effect of environmental factors on the sustainability of cultured production shellfish, we investigated the habitat characteristics of tidal flat (Namhae-po in Taean). We measured the physiochemical parameters (temperature, salanity, pH, dissolved oxygen and nutrients) and the geochemical characteristics (chemical oxygen demand, ignition loss, C/N ratio and C/S ratio). Surface sediments were collected from several site of tidal flat to examine the geochemical characteristics of both the benthic environment and heavy metal pollution. The grain size for research area of tidal flat were similar at the ratio of silt and clay in comparison with the other site of it. The C/N ratio was more than 5.0, reflecting the range arising from the mix of marine organism and organic matter. The C/S ratio (about 2.8) showed that survey area had anoxic or sub-anoxic bottom conditions. The enrichment factor (Ef) and index of accumulation rate (Igeo) of the metals showed that those research areas can be classified as heavily polluted, heavily to moderately polluted, or more or less unpolluted, respectively. Adult surf clam (Mactra veneriformis) density was highest at St. 2 (middle part of the Namhae-po), on the other hand, surf clam spat density was highest at St. 3 (lower part of the Namhae-po). Heavy rain, terrigenous suspended clay with fresh water from neighboring agricultural land, and severe high air temperature during summer could be thought as detrimental causes of spat and adult mortality in Namhae-po tidal flat. We suggested that the growth of shellfish in the tidal flat was effected by the various environmental conditions, so an improvement in the cultured method was needed.
Gametogenic Cycle and the Size at 50% of Group Sexual Maturity in Male Chlamys (Azumapecten) farreri nipponensis (Kuroda, 1932) (Bivalvia: Pectinidae) in Western Korea
Park, Ki Yeol ; Chung, Ee-Yung ; Lee, Ki-Young ; Park, Kwan Ha ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 65~76
DOI : 10.9710/kjm.2013.29.1.65
We investigated the gametogenic cycle and spawning seasons of the male Chlamys (Azumapecten) farreri nipponensis by qualitative and quantitative analyses, and also the size at 50% of group sexual maturity was calculated by the data of first sexual maturity. In this study, the male gametogenic cycle of this species by qualitative analysis was divided into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (July to September), and spent/inactive stage (August to January). The male gametogenic cycle showed similar patterns with monthly changes in the gonadosomatic index and condition index. Particularly, spawning in male scallop occurred once a year from July to September, unlike the spawning period of this species (from June to August) reported by the previous researchers. In quantitative statistical analysis using an image analyzer system, the patterns of monthly changes in the percent (%) of the areas occupied by spermatogenic stages to the testis areas in males showed a maximum in June, and then sharply dropped from July to September, 2006. From these data, it is apparent that the spawning season of C. (A.) farreri nipponensis occurred once per year from July to early September, indicating a unimodal gametogenic cycle during the year. Shell heights at 50% of group sexual maturity (RM50) fitted to an exponential equation were estimated to be 49.90 mm in males (considered to be one year old), and it was 100% for male scallops over 61.0 mm (considered to be two years old).
Seasonality of shellfish collection determined by growth-line analysis of the hard clam, Meretrix petechialis (Lamarck) recovered from the Daejuk-ri Shell Middens, Seosan, Korea
An, Deog-Im ; Ryu, Dong-Ki ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 77~82
DOI : 10.9710/kjm.2013.29.1.77
In this study, growth-line analysis was carried out on the hard clam (Meretrix petechialis) recovered from the Neolithic Age Daejuk-ri Shell Middens, Seosan, Korea, with aim to determine the seasonality of shellfish collection and site occupation. Growth increments of 206 specimens of the clam were examined. The marginal index (MI) of the archaeological specimens was calculated and was compared to the monthly marginal index of modern specimens under the assumption that the growth pattern was the same as it is today. MI of the archaeological specimens ranged from 0.13 to 1.29 and was divided into four categories: < 0.63, spring; 0.63-0.76, summer; 0.76-0.89, fall;
0.89, winter collection. As a result, of 206 specimens, 170 specimens (82.5%) represented spring, 18 (8.7%) summer, 10 (4.9%) fall and 8 (3.9%) winter collection. The results suggest year-round shellfish collection at the sites, with an emphasis on spring. Considering these results it can be inferred that the sites were occupied throughout the year.
Size-mass relationships for 4 freshwater snails (Gastropoda: Pleuroceridae) from the Guem River in Korea
Yoon, Ho Seop ; Choi, Sang Duk ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 83~85
DOI : 10.9710/kjm.2013.29.1.83
Size-mass relationships were estimated for 4 species belonging to genus Semisulcospira from the Guem River, Korea. These 4 freshwater snail species: Semisulcospira libertina, Semisulcospira coreana, Semisulcospira gottschei, and Semisulcospira forticosta. Between June and November 2011, samples were caught by dredge (mesh size 10mm of multifilament nylon, total length 4.35 m) at depths of < 2 m. Estimates of for parameter b of the size-mass relationship (W =
) ranged between 2.0072 (Semisulcospira libertina) and 2.3463 (Semisulcospira gottschei).
First record of Littorina (Littorina) kasaka (Littorinidae: Sorbeoconcha: Gastropoda) from Korea
Lee, Junsang ; Kil, Hyunjong ;
The Korean Journal of Malacology, volume 29, issue 1, 2013, Pages 87~89
DOI : 10.9710/kjm.2013.29.1.87
The Littorinid snail Littorina (Littorina) kasaka Reid, Zaslavskaya & Sergievsky, 1991, from Kandwon-do, Korea was recorded as new to the Korean molluscan fauna. Including the new recorded in this study, the family Littorinidae contained seven genera and 16 species in the Korean water.