Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Malacology
Journal Basic Information
Journal DOI :
The Malacological Society of Korea
Editor in Chief :
Volume & Issues
Volume 29, Issue 4 - Dec 2013
Volume 29, Issue 3 - Sep 2013
Volume 29, Issue 2 - Jun 2013
Volume 29, Issue 1 - Mar 2013
Selecting the target year
Geographic Variations of Three Fulvia mutica Populations
Kang, Seo-Kyeong ; Yoon, Jong-Man ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 163~169
DOI : 10.9710/kjm.2013.29.3.163
In the present study, the seven primers BION-33, BION-34, BION-37, BION-41, BION-44, BION-45 and BION-42 generated the total number of loci, average number of loci per lane and specific loci in Hongseong, Yeosu and Goheung population of F. mutica, respectively. 7 primers generated 19 specific loci in the Hongseong population, 29.3 in the Yeosu population and 23.1 in the Goheung population, respectively. Especially, the decamer primer BION-37 generated 7 unique loci to each population, which were identifying each population, approximately 700 bp in Hongseong population. In this study, the dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (HONGSEONG 01-HONGSEONG 07), cluster 2 (YEOSU 08-YEOSU 14) and cluster 3 (GOHEUNG 15-GOHEUNG 21). Among the twenty one cockles, the shortest genetic distance that displayed significant molecular differences was between individuals 17 and 19 from the Goheung population (genetic distance = 0.051), while the longest genetic distance among the twenty-one cockle individuals that displayed significant molecular differences was between individuals HONGSEONG no. 03 and YEOSU no. 12 (genetic distance = 0.616). Relatively, individuals of YEOSU population were fairly closely related to that of GOHEUNG population. Ultimately, PCR fragments revealed of in this study may be useful as a DNA marker the three geographic populations to distinguish.
Identification, sequence characterization and expression analysis of the arginine kinase gene in response to laminarin challenge from the Oriental land snail, Nesiohelix samarangae
Jeong, Ji Eun ; Lee, Yong Seok ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 171~179
DOI : 10.9710/kjm.2013.29.3.171
Arginine kinase (ArK) is known to play an important role in most invertebrates the level of ATP by phosphorylation of phosphagens in cell and immuninty in living organisms. ArK has been identified in many kinds of organisms ranging from invertebrate to vertebrate. However, no ArK gene has been cloned and investigated from N. samarangae. This leads us to identify ArK cDNA (NsArK) from the expressed sequence tag (EST) sequencing of N. samarangae. Sequence analysis indicated that the coding region of 1,065 bp contains 355 amino acid residues. Molecular phylogenetic analysis shows that NsArK had very high similarities with mollusca and arthropoda. In an attempt to investigate a potential role of NsArK in the digestive gland of N. samarangae, expression patterns were analyzed. RT-PCR analsysis shows that NsArK mRNA is induced in the rane of 1.2 fold at 6 hr by laminarin when compared with the control. The immunnologial and physiological role of NsArK remains to be further investigated in N. samarangae.
Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization
Nam, Bo-Hye ; Park, Eun-Mi ; Kim, Young-Ok ; Kim, Dong-Gyun ; Jee, Young-Ju ; Lee, Sang-Jun ; An, Cheul Min ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 181~187
DOI : 10.9710/kjm.2013.29.3.181
In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.
A Study on the Optimum Stocking Density of the Juvenile Abalone, Hailotis discus hannai Net Cage Culture or Indoor Tank Culture
Kim, Byeong-Hak ; Park, Min-Woo ; Son, Maeng-Hyun ; Kim, Tae-Ik ; Cho, Jae-Kwon ; Myeong, Jeong-In ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 189~195
DOI : 10.9710/kjm.2013.29.3.189
Experiments for net cage culture at sea were conducted in each
m in area and took the samples from four different densities: 150, 300, 450 and 600 per cross-sectional area (
) of shelter. The same stocking densities applied to indoor tank culture to investigate the growth and survival rate. The size of juvenile abalone sample was
mm for net cage culture and
mm or indoor tank. Feed such as raw brown sea mustard, raw kelp and dried kelp was sufficiently provided to the abalone. In net cage culture experiment, the growth of the spat of juvenile abalone was the fastest
mm in the 150 abalone cage per square meter (
), followed by the 300 abalone cage at
mm, 450 abalone cage at
mm and 600 abalone cage at
mm in order. In the meantime, in the indoor tank experiment, the 150 abalone indoor tank was the fastest
mm per square meter, followed by the 300 abalone tank at
mm, the 450 abalone tank at
mm and the 600 abalone tank at
mm in order. The survival rate was more than 97.9% in all the experiments, not showing a significant difference.
Effect of the concentrated-diatom of Caloneis schroederi and Rhaphoneis sp. for seedling production of Haliotis discus hannai
Kim, Seung-Hyern ; Kim, Mi Jeong ; Park, Se Jin ; Hur, Sung Bum ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 197~205
DOI : 10.9710/kjm.2013.29.3.197
Even though seedling production of Haliotis discus hannai has fully developed, the culture of benthic diatom as a live food for larvae is still a barrier to solve in commercial hatchery. The farmer depends on mixed microalgae which are non-selectively attached on the plate by flowing of natural seawater. The adequate diatom on the plate for the larvae in terms of quality and quantity is always significant bottleneck in the hatchery. In this study, two benthic diatom species, Caloneis schroederi and Rhaphoneis sp. were separately cultured in mass and inoculated to four tons' settlement tank of the larvae. And the larvae and the spats were cultured for nineteen and nine weeks, respectively. The result on seedling production of H. discus hannai with this method was compared to that of the farmer's traditional method as a control. With regard to variation of species composition of benthic diatom on the plate, C. schroederi and Rhaphoneis sp. were dominant for first three weeks after inoculation. But the diverse diatoms mainly, Navicula, Amphora, Cylindrotheca, Licmophora, Pleurosigma began to attache on the plate from the 4th week. The larvae attached 2.5 times more in C. schroederi tank than in the control tank. The final total biomass of the seeds in Rhaphoneis sp. tank was 3.2 times more than that of the control tank. The retared-spats in the seedling production also showed significantly higher growth and survival in the spat fed Rhaphoneis sp. or C. schroederi than those in the control group. We suggest that C. schroederi is proper for settlement of the larvae and Rhaphoneis sp. is appropriate for the growth of the larvae and spats in the commercial hatchery of H. discus hannai.
Changes in density and culture conditions of the Manila Clam Ruditapes philippinarum on the West coast of Korea
Park, Kwang-Jae ; Song, Jae-Hee ; Choi, Yoon-Seok ; An, Kyoung-Ho ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 207~216
DOI : 10.9710/kjm.2013.29.3.207
Changes in density of manila clam, Ruditapes philippinarum to environment, catch and recruitment were studied in a few stations (Seonjae, Seongam, Hwangdo and Padori) in the West coast area of Korea from January, 2007 to December, 2009. Water temperature, salinity, dissolved oxygen and pH in the study stations were 0.8 to
, 22.1 to 33.7 psu, 5.0 to 12.0 mg/L and 7.39 to 8.99, respectively. The concentrations of dissolved inorganic nitrogen, phosphate and silicate were 0.016 to 1.281 mg/L, 0.004 to 0.093 mg/L and 0.016 to 1.617 mg/L, respectively. Chlorophyll-a ranged from 0.2 to 12.1
, respectively. Substrata were mainly composed of muddy sand and very poorly sorted in Padori, muddy sand and well sorted in Seonjae and Hwangdo, gravelly muddy sand and poorly sorted in Seongam. Density was high in Seonjae and Seongam, but low in Hwangdo and Padori. In clam culture station, in which spat was naturally produced without sowing seedlings, the living density was decreased by increasing of death and a catch of shellfish, and recruitment was changed. Also, Density affected condition factor and shape of clam. Condition factor was the highest in Hwangdo, in which temperature in the winter and chlorophyll-a were high, and was the lowest in Padori. In the shape of clam, the shape in Seongam was a elongated form, but in Padori was a stunted form.
Morphological and physiological comparison between triploid and diploid Pacific Oyster, Crassostrea gigas
Kim, Su Kyoung ; Shim, Na Young ; Lee, Won Young ; Choi, Min Seop ; Choi, Eun Hee ; Lim, Hyun Jeong ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 217~223
DOI : 10.9710/kjm.2013.29.3.217
The morphological relations and physiological characteristics of the triploid and diploid oysters (Crassostrea gigas) in Taean area, west coast of Korea, were investigated from May 2012 to April 2013. Mophometric analysis indicated that the triploid oysters have the same shell length to shell height ratio but higher shell depth to shell height ratio than diploids. Consistent with morphological characteristics, triploid oysters showed greater values of fatness, condition index and RNA/DNA ratio during the period of experiment. The DNA concentration in adductor muscle and mantle of triploid were either lower or equal to the nucleic acids of diploid. However, RNA/DNA ratio were significantly higher than diploid. It appears that RNA/DNA ratio could be a useful indicator of health condition of triploid and diploid oysters when taken in correlation with the morphological indices.
Recovery Rate and Histological Changes in the Gills of Juvenile Abalone Haliotis discus hannai by Exposure Time of Different Water Temperatures and Salinities
Park, Mi Seon ; Kim, Seong-Hee ; Lim, Han Kyu ; Min, Byung Hwa ; Chang, Young Jin ; Jeong, Min Hwan ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 225~232
DOI : 10.9710/kjm.2013.29.3.225
This study looked into recovery rate and histological changes in the gills of juvenile abalone Haliotis discus hannai by exposure time (3, 6, 12, 24 and 48 h) of different water temperatures (15, 20 and
) and salinities (30, 25, 20 and 15 psu) to understand reasons for the death of abalone exposed by low salinity water. In each water temperature, abalone spats that were exposed to low salinity water (less than 20 psu) for over 6 hours showed decrease in survival rate during recovery and those were exposed at the salinity of 15 psu for more than 24 hours all died. Histological observation showed expansion or damage of gills of the species which were exposed at less than 20 psu for over 6 hours. In case of abalones exposed at the salinity of 15 psu for over 24 hours, most gill tissues were destroyed. This result was glaringly obvious at a higher water temperature, lower salinity and longer exposure time. Accordingly, suffocation caused by damage of gills considered one of direct causes of the death.
Comparison of formulated feed and two seaweed-based diets on growth of Pacific abalone (Haliotis discus hannai)
Kim, Sung Yeon ; Park, Choul-Ji ; Nam, Won Sick ; Kim, Jae Mok ; Lee, Jeong-Ho ; Noh, Jae Koo ; Kim, Hyun Chul ; Park, Jong Won ; Hwang, In Jun ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 233~238
DOI : 10.9710/kjm.2013.29.3.233
The effect of three different diets (formulated diet, FD; Undaria pinnatifida, UP; Laminaria japonica, LJ) on growth in the Haliotis discus hannai for 90 days was investigated. The shell length of UP (
mm) and LJ (
mm) were significantly faster than those of FD (
mm) (P < 0.05). However, shell breadth and total weight were no significant difference among three diets. On the other hand, the weight gain of FD (16.65% for 0-45day and 25.71% for 45-90day) tend to have higher than those of UP (14.57% and 23.30%) and LJ (12.65% and 24.51%). This results shows that seaweed diets (UP and LJ) help the shell growth of abalone and formulated diet (FD) help the weight gain of abalone. Therefore, the growth of shell and muscle will depend on different diets.
A report on the mass summer mortalities of the farmed Pacific oysters, Crassostrea gigas and Bay scallops Argopecten irradians in the local waters of Goseong Bay, Korea
Han, Jong Cheol ; Jo, Qtae ; Park, Young Cheol ; Park, Tae Gyu ; Lee, Deok Chan ; Cho, Kee-Chae ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 239~244
DOI : 10.9710/kjm.2013.29.3.239
Mass mortalities of farmed shellfish, mostly in summer season, thus named mass summer mortalities, have been a global issue in shellfish aquaculture. The 2013 mass summer mortalities in the confined waters of Goseong Bay, Goseong, Korea were quite a unique and intensive for two farmed species, the Pacific oysters, Crassostrea gigas, and bay scallops, Argopecten irradians. The mortalities were progressive from the bottom of the suspended oysters and caged scallops in the waters, reaching up to 80% for the oyster and 95% for the scallop in about 20 days after the first occurrence, early August, 2013. We monitored a wide range of environmental factors, including water temperature, dissolved oxygen (DO), salinity, turbidity, acidity (pH), organic and inorganic matters, chemical oxygen demand (COD), suspected pathogenic agent, and phytoplankton composition throughout the water column where the two species were suspended or caged. Our survey concluded that the hypoxia or anoxia might be a major cause of the mortalities. Here, we detailed the mortalities and ways to arrive at the conclusion.
Effects of Water Temperature on The Mass Mortality of Pacific Oyster, Crassostrea gigas in Gamak Bay
Kim, Chul Won ; Oh, Hyun Ju ; Shin, Yun Kyung ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 245~250
DOI : 10.9710/kjm.2013.29.3.245
We investigated the factors of mass-mortality in terms of water temperature and prey, in order to prevent the mass-mortality of cultured oysters at Gamak Bay in Yeosu City in 2007. The real-time water temperature was recorded as high, 28 to 31C, during late August. Nutrients, Dissolved Inorganic Nitrogen (DIN) and Dissolved Inorganic Phosphate (DIP) were downed in September. The analyzed results of chlorophyll a content were 0.78-1.50
and phytoplankton for food resources was 81 cells
, both were low. The finding here indicate that Gamak Bay is in an oligotrophic state. The mass-mortality of cultured oysters occurred 43.6% in Gamak Bay. The mortality rate of oyster were above 67.0%, at Wanpo, however, it was showed 18.3% at Gumchun. Therefore, we believe the mass-mortality of cultured oysters at Gamak Bay comes from the destruction of bio-rhythms due to high water temperature and quantitatively and qualitatively decreasing food resources due to the limitation of nutrients.
Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm
Park, Mi Seon ; Min, Byung Hwa ; Park, Jung Jun ; Lim, Hyun Jeong ; Myeong, Jeong-In ; Jeong, Min Hwan ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 251~258
DOI : 10.9710/kjm.2013.29.3.251
This study aims to find out a suitable cryoprotective agent (CPA) for cryopreservation and its optimum concentration in order to conduct planned artificial seed production of Pacific oyster, Crassostrea gigas and to preserve superior sperm. For this, we tried to understand toxicity and the effect of cryopreservation by CPA type and concentrations first and then looked into cell damage of the sperm after thawing. Toxicity analysis on the sperm of Pacific oyster according to different CPA and immersion time shows that dimethyl sulfoxide (DMSO) comes first when it comes to survival rate and mobility followed by ethylene glycol (EG), glycerol and methanol. To identify the optimum CPA and its level, filtered seawater was used as a diluent before cryopreservation for 30 days. As a result, cryopreserved sperm of Pacific oyster with 15% of DMSO showed the highest survival rate and activation. Also, we observed the cryopreserved and thawed sperm with Scanning electron micrographs by CPAs and concentrations. Consequently, DSMO showed the lowest cell damage followed by EG, methanol, glycerol and the level was 15, 20, 10, 5% respectively. In a nutshell, it is proven that the optimum CPA and its level is 15% of DMSO.
Three Unrecorded Triphorid Snails of Genus Triphora (Caenogastropoda, Triphoridae) from Korea
Kil, Hyun-Jong ; Lee, Yong-Seok ; Lee, Jun-Sang ;
The Korean Journal of Malacology, volume 29, issue 3, 2013, Pages 259~262
DOI : 10.9710/kjm.2013.29.3.259
The specimens of three unrecorded triphorid snails, Mastonia millepunctata, Monophorus testaceus, and Obesula turricula were collected from Jeju-do, Korea and diagnostic characters were analysed. As a result, the family Triphoridae in Korea turned out to be 18 species revised species catalogue are listed.