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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Ginseng Research
Journal Basic Information
Journal DOI :
The Korean Society of Ginseng
Editor in Chief :
Volume & Issues
Volume 14, Issue 3 - Dec 1990
Volume 14, Issue 2 - Aug 1990
Volume 14, Issue 1 - Apr 1990
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Effect of Acidic Polysaccharide of Red Ginseng on Lipolytic action of Toxohormone-L from Cancerous Ascites Fluid
Lee, Sunh-Dong ; Kameda, Kenji ; Takaku, Takeshi ; Sekiya, Keizo ; Hirose, Kumi ; Ohtani, Kazuhiro ; Tanaka, Osamu ; Okuda, Hiromichi ;
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 1~5
Toxohorome-L is a lipolytic factor found in ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. A substance that inhibited the lipolytic action of Toxohormone-L was isoialed from red ginseng powder. This substance had a pectin-like o 1, 4-pollrgalacturonan backbone with some acetoxyl groups, and so was an acidic polysaccharide. It inhibited Toxohormone-L-induced lipolysis in a dose dependent manner at concentrations higher than 10
/ml. Keywords Panax ginseng C.A. Meyer, red ginseng toxohormone-L
Studies on Enzyme Activities Responsible for Biosynthesis of Putrescine in Ginseng(Panax ginseng C.A. Meyer)
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 6~9
Specific activities of ADC and ODC from 2-4 year old ones were higher than that from seedlings whereas those activities were not changed significantly from 2 to 4 years. Generally, activity of ADC was predorminant compared to that of ODC. Free arginine content in roots was much higher than that of leaves. And arginase specific activity from roots was higher than that of leaves. Cumulative results suggest that putrscine formation from ornithine in roots may be more effective than leaves and contribute to putrescine biosynthesis to some extract.
Inhibitory Effect of Crude Acidic Polysaccharide of Korean Ginseng on Lipolytic Action of Toxohormone-L from Cancerous Ascites Fluid
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 10~13
Effect of an acidic polysaccharide fraction In Korean white and red ginseng on lipolytic action of Toxohormone-L was studied. Crude acidic polysaccharide fraction was extracted from main and lateral root of Korean white and red ginseng separately and purified several times. Inhibitory effect of crude polysaccharide fraction was determined by unit (1 unit is loft inhibition rate per Is sample). Yields of purified crude polysaccharide fraction of main and lateral root of red ginseng were 2.9 and 2.2 times higher than those of white ginseng, respectively. Inhibitory effects of main root of white and red ginseng, 11.hen final reaction concentrations of sample were 50, 100, 200, 500
g/ml, were 37.2가 and 23.7% higher than those of lateral root of white and red ginseng. Inhibitory effect of main root of red ginseng was 2.3 times higher than that of white ginseng.
Purification and Characterization of Invertase from Korean Ginseng Panax ginseng
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 14~20
In An invertase (EC 126.96.36.199) was extracted from Korean giseng (Panax ginseng C.A. Meyer) with distilled tvater The ginseng invertase was purified about 62.6 folds purified by procedures including ammonium sulfate fractionation , DEAE-cellulofine chromatography and gelfiltrations through Sephadex G-75 and the recovery of enzyme activity was 11.1%. The homogeneity of the purified enzyme was probed by polyacrylamide gel disc electrophoresis. The purifled enzyme was divided into two different subunits by treating with a mixture of SDS and 2-mercautoethanol, and the molecular weight of the large subunit was estimatedtobe 116,000 and that of the small one to be 14,000. The optimal VH and temperature of the enzyme were pH 6 and 45
, respectively. The enzyme hydrolyzed specifically the hydrolyzation of the -fructofuranosides such as sucrose, raffinose and inulin. The Km values of the enzyme for sucrose and raffinose were determined to be 0.85 and 0.6 mM, respectively.
Chemical composition and Stabilities of Invertase from Korean Ginseng, Panax ginseng
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 21~26
The chemical composition and stabilities of the purified ginseng invertase were investigated. The purified enzyme was found to be a glycoprotein composed of 80.2% protein and 19.7% total sugar. The protein component of the enzyme was composed of acidic amino acid (9.3%), basic amino acid (48.9%), nonpolar amino acid (21.4%), polar amino acid (20.4%) and 6.1% S-containing amino acid. It showed especially high contents of histidine and serine. The enzyme was inactivated almost completely by the treatment with some proteases (papain, pepsin. trypsin, pancreatin and microbial alkaline pretense) and protein denatllrants (8M urea and 6M guanidine-HC1), bolt not with glyrosidase (
-amylase. glcoamylese and cellullase). btonosaccharides sllch as glilrose, fructose, galactose and mannose did not exert any influence on the enzyme activity. The activity of the enzyme was inhibited by Ag+, Mn2+, Hg2+, Zn2+ and Al3+, whereas Ca2+, Mg2+, Ba2+ and Fe3+ gave rather activating effects on the enzyme activity. The enzyme was relatively stable in the VH range of VH 6 and 8, and at the temperatures below 35
Structural Study on an Permethyl Ether of a New Polyphenolic Compound Isolated from Panax ginseng
Wi, Jae-Jun ; Park, Jong-Dae ; Kim, Man-Uk ;
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 27~29
A Polyphenolic Compound was isolated from ethyl acetate fraction of Panax ginseng. And the chemical structure of its permethyl ether was suggested as 6-[2(3,4-dimethoxyphenyl)ethenyl]-5-(3,4-dimethoxyphenyl)2,8,10-trimethoxybenzo[a] xanthen-9-one by spectroscopic and chemical degradative evidences.
Effect of Ginseng Extracts on Photosensitized Peroxidation of Human Erythrocyte Ghosts
Baek, Tae-Hong ; Cheon, Hyeon-Ja ; Gang, Byeong-Su ;
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 30~35
The photooxidation of human erythrocyte ghosts has been studied and the effects of ginseng water extract on it have been investigated. In the presence of photosensitizer, rose bengal, human erythrocyte ghosts have caused photooxidation by lO2 and produced lip)id hydroperoxides. Ginseng water extract and d-
-tocofherol have inhibited photooxidation, ,whereas ascorbic acid has developed in low concentrations but inhibited in high concentrations. On the other hand, amounts of lipid hydroperoxide produced by photooxidation were decreased by addition of catalase after irradiation and according to sequential addition of ginseng water extract into it, the formation of lipid hydrolleroxide was decreased simultaneously.
Effect of Shading on the Quality of Raw, Red and White Ginseng and the Contents of Some Minerals in Ginseng Roots
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 36~43
The quality of raw, red and white ginseng and the contents of some minerals were examined using 6-year-old ginseng roots produced in different shades, thatch and polyethylene net (P..E.). The yield of first and second grade ginseng roots was higher in the thatch shade than in the P.E. shade. The smaller sizes of ginseng routs were probably due to loss of ginseng yield called by alternaria blight in the third and fourth years, and lower quality was dale to more rusty roots in the P.E. shading. For red ginseng. rates of heaven and earth grades were higher in the P.E. than thatch shade. producing red ginseng with less inside cavity Production of white ginseng was higher in the thatch shade than in the P.E. shade. showing a higher yield, better piece grade, lower inside crack and better quality index in the thatch. The contents of some minerals such as K, Ca, Mg and Mn of fine ginseng roots differed between the two shades, some of which had a significant correlation with the quality indices of white ginseng. Keywords Thatch shade, polyethylene shade, alternaria blight, rulsty root, quality of ginseng.
Effects of Some Liming Materials Application on the Alleviation of the Symptoms of Mn Toxicity Inculding Marginal Leaf Chlorosis
Lee, Tae-Su ; Kim, Myeong-Su ; Hong, Sun-Geun ;
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 44~49
Effect of liming material application on the prevention or cure of Mn toxicity symptoms including marginal leaf chlorosis in 3-year-old ginseng plants grown in acidic soils were investigated. It was found that the ratio of Fe to Mn was above about 0.5 and the ratio of Mn to Fe was below about 2.00 in 4-year old ginseng leaves when liming materials were applied in field experiments. It was apparent that the occurrence of marginal leaf chlorosis was decreasing affected by application of Ca, Mg and Fe. The appearance of marginal leaf chlorosis was decreased to about 78 percent in 4-year-old ginseng and to about 69 percent in 5-year-old ginseng, and then the fresh root weight was increased up to 66 percent in 4-year ginseng plants.
Comparison of Resistance of Root Rot Caused by Fusarium solani in Ginseng Breeding Lines
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 50~56
Root-rot of ginseng caused by Fusarium solani is one of the most obstacles to ginseng cultivation. We evaluated some inoculating techniques of ginseng with Fusarium solani, for selection of disease resistant breeding lines. The most effective inoculating techniques evaluated were inserting toothpicks colonized by F. solani into the seedling roots in laboratory test and dusting seedlings with vermiculite after dipping in conidial sllspension and then replanting method in field test. The resistance to diseased by F. solani was lines of 82022 and 82066 in laboratory test. 82920-1 and 78093 in field test.
Effects of Light and Photosynthetic Electron Transport System on the Generation of Singlet Oxygen (
) in Ginseng Thylakoid Membrane
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 57~62
In order to Investigate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), studies on the generation of singlet oxygen (1O2) and the photooxidation of the pigments were carried out in comparison with the ones of soybean (G1ycine max L). The studies were mainly focalized on the effects of light intensity, light intensity, inhibitor and electron donor/acceptor of the Photosynthetic electron transport system. When we measured the amounts of 1O2 generated in the thylakoids of ginseng and soybean by the irradiation of light (300 w/m2) as a function its time. It was identified that a higher amount of 1O2 was formed in the ginseng thylakoid than the case of soybean. A generation ratio of lO2 between ginseng and soybean sltbstantially identical in the range of light intensities 50∼150w/m2 However much higher amount of 1O2 was generated in ginseng by irradiation of strong intensity of light (200 500w/m2). Wave length dependency on the generation of 1O2 and the pigment photooxidation was observed on ginseng thylakoids; red light (600-700 nm) gave a maximum effect in the contrast with blur green light (400-60 nm). When the ginseng thylalioid was treated with the electron donor (Mn2+) and acceptors (DCPIP, FeCy) of the photosynthetic electron transport system. a drastic inhibition of 1O2 generation was observed. However, treatment with its inhibitors (DCMU, KCW) activated 1O2 generation. An interesting fact that an electron donor or acceptor of the photosystem II(P680) Inhibited 1O2 generation, suggests an intimate relationship between 1O2 generation and photosystem II.
Possible Factors Affecting Crack Development in Ginseng Roots
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 63~66
The development of cracking in ginseng roots was studied to elucidate the factors affecting it in the field. The cracking of 6-year-old ginseng roots harvested in late October callld be induced in 2 days after soaking in water or in watersatllrated soil. However, it callld be slowed down by approximately 10 days when the roots were kept at a low temperature(5
). Roots excavated in late May did not develop cracking tinder the conditions tested. There was no significant difference between rice-straw shade and P.E. net shade in the rate of cracked ginseng roots. Which varied with ginseng varieties in relation to shade material. The rate of cracked roots was about 40% in 6-year plants, but it was extremely high (55.6%) in rusty roots. Healing of the wound formed by cracking was generally low but varied between harvest years.
Effect of Acidic Polysaccharide of Korean Red ginseng on Lipolytic Action of Toxohormone-L from Canceroils Ascites Flilid
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 67~73
Toxohormone-L is a lipolytlc factor, found in ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. A substance that inhibited the lipolytic action of Toxohormone-L was isolated from Korean red ginseng powder. This substance had a pectin-like a-1,4-polygalacturonan backbone with some acetoxyl groups, and so was an acidic polysaccharide. Acidic polysaccharide was found to inhibit significantly toxohormone-L-induced lipolysis at its concentration of 10
인삼의 효능과 성분에 대한 연구의 최근 경향
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 74~80
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 81~88
식물호르몬과 광이 인삼(Panax ginseng C. A. Meyer)의 Polyamine 함량과 Arginine Decarboxylase 활성도에 미치는 영향
Journal of Ginseng Research, volume 14, issue 1, 1990, Pages 89~96