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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Ginseng Research
Journal Basic Information
Journal DOI :
The Korean Society of Ginseng
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 2000
Volume 24, Issue 3 - Sep 2000
Volume 24, Issue 2 - Jun 2000
Volume 24, Issue 1 - Mar 2000
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Effect of Ultrasonic treatment on the Isolation of the Chlamydospores of Cylindrocarpon destructans Causing Root rot of Panax ginseng
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 53~57
Chlamydospores were isolated from hyphae of Cylindrocanon destmctans by homogenization and/or ultrasonic treatment. Rate of the isolated chlamydospores by the homogenization with glass tissue grinder were 9.8% of all total chlamydospores formed in the culture of C. destructans. The length of mycelial fragments after the homogenization was about 400㎛ They were, however, formed in clusters of the chlamydospores and the mycelia The rate of the isolated chlamydospores from additional ultrasonic treatment after the homogenization of the mycelia were 74.3%. The length of mycelial fragments with the ultrasonic treatment was about 20 fm and chlamydospores seemed to be isolated from the mycelial mats and dispersed evenly in the culture. The numbers of chlamydospore in a catena were 1 to 8 cells after the homogenization on potato dextrose agar (PDA). Meanwhile the numbers of them after added ultrasonic treatment were 1 to 4 cells. Germination percentages of the isolated chlamydospores from the ultrasonic treatment were 46.8% after incubation of 2 days on PDA at 20。C and 60.7% after incubation of 13 days at 5。C, respectively. Germination rate of chlamydospores to the total chlamydospores produced by the ultrasonic treatment was 55.8%. However, it was increased to 74% when it was measured in the germinated catenae to the total catenae.
Optimization of Submerged Culture Conditions for the Production of Ginseng Root Using Response Surface Method
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 58~63
To develop the production of ginseng root using plant tissue culture technology, submerged culture conditions were optimized by means of the fractional factorial design with 4 factors and 3 levels by a RSM computer program. The ginseng (Panax ginseng C. A. Meyer) roots induced by plant growth regulators were cultured on SH medium and the effects of various pH of medium, sucrose concentration, nitrogen concentration and phosphate concentration on fresh weight of the ginseng root were investigated. The fresh weight of ginseng root increased with a decrease in nitrogen concentration and fresh weight of ginseng root varied from 1.00 to 2.33g under various conditions. The optimum pH of medium and sucrose concentration determined by a partial differentiation of the model equation, nitrogen and phosphate concentration were pH 5.6, sucrose 3.8%, nitrogen 50 mg/L and phosphate 80.7 mg/L, respectively. Under these conditions, the predicted growth of ginseng root was estimated to be 2.36g.
Determination of Total Phenolic Compounds from Korean Red Ginseng, and Their Extraction Conditions
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 64~67
This study was carried out to investigate the possible determination of total phenolic compounds by Folin-Denis method from Panax ginseng C.A. Meyer and their extraction conditions. It was possible to apply the method for determination of total phenolic compounds from Korean red ginseng. But 3 kinds of amino acids such as tyrosine, cystein and tryptophan, and 3 kinds of vitamins such as ascorbic acid, pyridoxin HCI and thiamine HCI affected strongly the colorization by the method. Effective alcoholic solvent for the extraction was 60% ethanol, appropriate extraction temperature, time and times were 40-80。C, 1-2 hours and 3 times, respectively
The Production of Ginsenosides from Ginseng Hairy Root by Treatment of the Chitin and Chitosan
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 68~73
To elucidate the effect of chitin and chitosan on the production of ginsenosides and growth, ginseng hairy root was cultured on the 1/2 MS medium supplemented with chitin and chitosan of various concentrations and culture period. The highest growth was obtained with 1 mg/L of chitin. However, the growth was inhibited by 20 mg/L or above. The contents and productivity of ginsenosides were the highest when ginseng hairy roots were cultured on 40 mg/L chitin and applied of the third-weeks of culture period. Ginseng hairy root culture with 1 mg/L of chitosan resulted in the best growth, but the highest ginsenosides level was appeared in 30 mg/L chitosan. Ginsenosides content was increased when it was treated at the forth-week after culture as 30 mg/L of chitosan.
Effects of Jasmonic acid and Methyl jasmonate on the Production of Ginsenosides in the Hairy Roots of Korean Ginseng (Panax ginseng C.A. Meyer)
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 74~78
To elucidate the effects of jasmonic acid and methyl jasmonate on the production of ginsenosides and growth, ginseng hairy root KGHR-8 clone was cultured on the 1/2 MS medium without growth regulators, which was supplemented with of various concentrations jasmonic acid and methyl jasmonate and culture period. The highest growth rate was obtained when 1
jasmonic acid and methyl jasmorlate were treated. However, the growth was inhibited at more than 30
of concentration. Treatment with high concen Dation of jasmonic acid (10
) and methyl jasmonate (50
) increased the contents and productivity of ginsenosides reversion of the growth inhibition. The highest contents and productivity of ginsenosides were appeared at 4 weeks after onset of the treatment of jasmonic acid and at 3 weeks in the case of methyl jasmonate.
Ginseng Saponin Prevents the LPS-induced TNE-
Production in Mice
Kim, Kyoung-Mi ; Kim, Hye-Ju ; Ryu, Jae-Ha ; Sohn, Dong-Hwan ;
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 79~82
Saponins, the major component of ginseng root, mediate the pharmacological action of the ginseng. It has been reported that ginseng roots have protective effect against various toxins. In this study, the effects of ginseng total saponin (GTS) on tumor necrosis factor-alpha (TNF-
) production induced by bacterial toxin was investigated. TNF-
level in lipopolysaccharides (LPS)-activated serum was remarkably reduced by intraperitoneal administration (50 mg/kg)of ginseng total saponin (GTS) into mice. The inhibitory effect against TNF-
production was not significant when GTS was given after the LPS injection, and by oral administration. These results suggested that ginseng root may have protective activity against liver damage accompanying the overproduction of TNF-
and GTS is the active component of ginseng.
Sucrose Synthase, UDP-glucose pyrophosphorylase and ADP-glucose Pyrophosphorylnse in Korea Ginseng Roots
Yelena V.Sundukova ; Lee, Mi-Ja ; Park, Hoon ;
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 83~88
The seasonal variation in the activity of sucrose synthase, ADP-glucose pyrophosphorylase and UDP-glucose pyrophosphorylase in roots of Panax ginseng C.A.Meyer have been studied. It was revealed that sucrose synthase and ADP-glucose pyrophosphorylase are adaptive enzymes and can serve as markers of sink strength, while UDP-glucose pyrophosphorylase is the maintenance enzyme. The average day temperature exceeded 24。C appeared to cause the disturbance in refilling process, affecting the starch synthesis. Study on the dependence of oxygen consumption in stele tissue with temperature revealed the sharp accelerating of this process after 24。C.
Exo-O-Glycosylhydrolases in Korea Ginseng Roots
Yelena V.Sundukova ; Lee, Mi-Ja ; Park, Hoon ;
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 89~93
WB were screening the stele and the cortex of the ginseng roots (Panax ginseng C.A.Meyer) on the exo-0-glycosylhydrolase activities during vegetation period of 1999 year. The following p-nitrophenylglycosides were used to test exe-0-glycosylhydrolase activities:
-L-mannosi-dase , N- acetyl-
-D-fuco sidase were found in both partsof ginseng roots. Their contents during the vegetation period were shown to differ considerably, being dependent not only on plant development stage but on plant tissue and environmental conditions too.
Protective Effect of Panax ginseng Against Tetracycline Toxicity in Rats
Nada, Somaia A. ; Arbid, Mahmoud S. ;
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 94~98
Tetracycline hydrochloride (TC) caused 100%, 50% and 20% mortality rates among rats injected with 40 mg, 30 mg and 20 mg/100g. b.w. respectively; while the morta]ity rates were decreased to 50%, 20% and 10% when Panax ginseng (2 mg/100g. b.w.) injected with TC during 72 hrs. post-injection. Subacute-toxicity study demonstrated that TC caused severe hepato-nephrotoxicity (demonstrated by biochemical analysis of serum including: transferases , alkaline phosphatase, total protein, glucose, cholesterol urea and creatinine) in rats injected i.p. with 10 mg and 5 mg/100g. b.w. for 7 days of daily injection . These signes of toxicity were greatly diminished by P. ginseng addition to TC doses.
Usage and Dosage of Ginseng Radix (panax ginseng C. A. Meyer) Based upon Traditional and Recent Scientific Clinical Applications
Journal of Ginseng Research, volume 24, issue 2, 2000, Pages 99~105
Ginsenf Radix (Panax ginseng C. A. Meyer) has been traditionally used as a herbal medicine for many therapeutic or prophylactic purposes in the oriental countries such as Korea, Japan and China for at least two thousand years and also extensively studied in the modern scientific field of chemistry, biochemistry and pharmacology. The herb is now also indicated for use as tonic or a prophylactic and restorative agent for enhancement of mental and physical capacities, in case of weahess exhaustion tiredness loss of concentration, impotence, cold limbs, during illiness anuor convalescence. Ginseng is commonly used in the form of detections, extract and powderl and ginseng products, in the form of capsules tablets and drinks. And also ginseng radix has been widely traditionally prescribed as an important comuonents of manny Chinese prescriptions or alone in various diseases and for health with its different dosages. Nowadays since rinsenf can be generally classified into food or medicine in many nations, it is very difficult to give any exact desnition on the dosage, which may be of particular importance in clinical applications. In addition, the establishment of the reasonable dosage is currently of great significance to meet the demand for such wide applications. Accordingly in this review paper we summarized the dosage of ginseng on the basis of oriental medical books oriental and western pharmacopeias and modern scientific clinical data. The recent survey demonstrated that the averare dosare of finsenf is considered to be three to four grams per day unless prescribed apart, while one to two grams per day in western countries from the western viewpoint of classification of ginseng as a medicine, surrorted by the dosage of not more than one gram per day in most clinical studies. For that reason, it seems likely that the dosage in western countries is ascribed to the safety of ginseng considering side or unwanted effects. Consequently whether the differences of dosage between oriental and western countries depend on dietary habits and races should be closely investigated. Besides, further studies on the pharmacokinetics and bioavailability of ginseng components in clinical trials need to be done to decide optimum dosage of ginseng.