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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Herbal Formula Science
Journal Basic Information
Journal DOI :
The Korean Journal of Oriental Medical Prescription
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Volume & Issues
Volume 10, Issue 2 - Dec 2002
Volume 10, Issue 1 - Jun 2002
Selecting the target year
A Personal Perspective and Our Role in Korean Oriental Medicine
Kang Shun-Su ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 1~4
The development of Korean Oriental medicine is based upon the accumulation of experience and knowledge gathered over the centuries. The approaches taken are holistic and empirical. There is a need to understand their actions at molecular levels with more rational, objective and scientific studies. Today it appears that Chronic and age-associated diceases may be multifactorial and hence more complex. A different approach may be required. One claimed usage of Korean Oriental medicine is for the treatment and prevention of chronic and age-associated illnesses. Some of the botanical formulas used for this purpose were discovered thousands of years ago and continue to be used today. There are indications that these formulas may indeed be helpful in the treatment or prevention of chronic diseases. This multi-component medicine could not only be very useful meeting the unmet clinical needs but for defining a more synergistic therapy that supports and maintains the bodies natural curative abilities. The potential usefulness of Korean Oriental medicine embodies the belief of maintaining healthy homeostasis of the body through the proper balance of a mixture of chemical at different organs or tissues. This concept is different from western medicine and implies that multiple compounds may act on multiple mechanisms of action to maintain the balance of the complex web of biology. This is very important in view of sciences current direction to integrate fragmented information to develop future medicines. The western and eastern approaches to human health and disease are complementary to each other. The best approach in developing future medicines is to integrate both approaches.
Studies on application of Cimicifuga heracleifolia blended Prescriptions from Donguibogam
Park Sang-Nam ; Kook Yoon-Bum ; Lee Jang-Chun ; Yun Young-Gab ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 5~46
This report describes the studies as follows through the research into the data of Cimicifuga heraclefolia Komarov blended prescriptions from Donguibogam. Cimicifuga heracleifola Komarov blended prescriptions are utilized for 39 therapeutic ranges. such as in dentistry, onclology, and 16 therapeutic ranges occupy over 84% totally, from dentistry to nasal disease. Cimicifuga heracleifola Komarov blended prescriptions use in the symptpms resulting from dentistry, intestine, internal disease, and they are used 22 disease also. The main pathologies of Cimicifuga heracleifola Komarov blended prescriptions found weakness in the digestive system, heat of stomach, wind of stomach in the splanchnology, and pathologies of wind-heat, wind-moisture, cold, excluding they are using for energy-blood, weakness, internal disease. The actual amount of Cimicifuga heracleifola Komarov blended has a wide capacity from 2 pun to 5 don, 1 don is broadlly used by 26%. Through the research into the main factors that we know Cimicifuga heracleifola Komarov blended prescriptions are listed in Yangmyungkyung, as they are regularly used as disease treatment and increasing the level of the general energy of the body, relaxing a skin, increasing stamina, detoxication, eliminating tumor, widely.
A study of medical treatment for So-gal(消渴) by drink composed of The one herb medicine
Park Choon-Ha ; Wei Tung-Sheun ; Kim Yong-Sung ; Lee Kyung-Hwan ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 47~55
In Oriental medicine, So-gal(消渴) is similar to Diabetes Mellitus in the symptoms. In the medical treatment of So-gal(消渴), one kind of herb medicine is recorded in ancent medicine literatures. That herb medicine is a juice of vegetable drunken with herb medicine of So-gal(消渴). Milk(牛乳汁), human milk(人乳汁), gam-ja-jup(甘蔗汁), wo-jul-jup(藕節汁), li-jup(梨汁), sang-gang-jup(生薑汁) etc. is that. In that juices, Milk(牛乳汁), human milk(人乳汁), gam-ja-jup(甘蔗汁), wo-jul-jup(藕節汁), li-jup(梨汁) have sweety tastes, and cold nature. So they cure So-gal(消渴) come from dry and hot nature. Sang-gang-jup(生薑汁) is different with others in the taste and nature that sweety and hot taste and hot nature. Sang-gang-jup(生薑汁) cure the sequela of So-gal(消渴) by his hot nature. We think In Diabetes Mellitus, that juice can be used by drink for the treatment of diet because of simility between So-gal(消渴) and DM.
Effects of Shihoieokgan-Tang with Other Herbs and Formulae Pretreatment on the Change of Corticosterone Induced by Immobilization Stress in Mice
Kim Hyun-Jun ; Lee Tae-Hee ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 57~72
To investigate the effect of Qi promoting-agents, clearing heat of deficiency type-agent, clearing heat of excessive type-agent, warming interior part of body-agent and Shihoieokgan-Tang against the immobilization stress, Cypri Rhizama, Auranti pericarpium, Aucklandiae Radix and Bupleuri Radix, Soyo-San, Hwangyeonhaedok-Tang, Geongangbuja-Tang and Shihoieokgan-Tang was administered intragastically 1hr prior to immobilizaiton stress and the change of corticosterone level has been measured by the flurometric method. The results obtained are as follows: 1. Qi promoting-agents(Cypri Rhizama, Auranti pericarpium, Aucklandiae Radix and Bupleuri Radix) showed mildly reducing effect or did not shown the reducing effect against the elevated coritocostereone level induced by the immobilization stress in the mice. 2. Hwangyeonhaedok-Tang, Gungangbuza-Tang, and Soyo-San did not show the reducing effect against the elevated corticostereone level induced by the immobilization stress in the mice. 3. Shihoieokgan-San was effective against the elevated corticosterone level induced by the immobililzation stress at 1g／kg, 3g／kg respectively. Consequently these data proved that Qi pomoting-agents as single herb(Cypri Rhizama, Auranti pericarpium, Aucklandiae Radix and Bupleuri Radix), clearing heat of deficiency type-agent(Soyo-San), warming interior part of the body(Gungangbuza-Tang), clearing heat of excessive type-agent(Hwangyeonhaedok-Tang) have no effect to decrease the elevated corticosterone level in mice exposed to the immobilization stress. Therefore our study revealed that Shihoieokgan-San which has the effects of herbs and formulae mentioned above decreased the elevated corticosterone level in mice exposed to the immobilization stress significantly.
Single dose toxicity study of Jengjengamiyjintang in rats
Kim Sang-Chan ; Kwon Young-Kyu ; Byun Jun-Seok ; Kim Han-Kyun ; Byun Sung-Hui ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 73~83
The single dose toxicity of Jengjengamiyjintang, a herbal drug for duodenal ulcer was evaluated in male and female Sprague-Dawley (SD) rats. Jengjengamiyjintang was once administered to both sexes of rats at the dose levels of 2000, 1000, 500, 250 and 125mg／kg for oral route according to KFDA guidelines for single dose toxicity test (1999-61). In addition, vehicle control group was added in order to compare clinical signs, body weight changes and abnormal necropsy findings. After single administration, clinical signs were observed every twice a day for 14 days and body weights were measured 5 times including initial measurement on day 0. When observation period was over, the animals were sacrificed and macroscopic examination of major organs was conducted. Neither significant clinical signs nor death after administration was observed during the observation periods except for soft feces or diarrhea that were restricted to Day 1 of 2000 and 1000mg/kg-dosing groups. Although some accidental findings such as gross and histopathological changes of lung that were demonstrated in some animals of all experimental groups including vehicle control group, no abnormal necropsy findings and changes of body weight were observed at terminal necropsy in both sexes. From these results, it is considered that
of Jengjengamiyjintang is over 2000mg／kg in oral administration in both sexes of rats and approximated lethal dose was considered over 2000mg/kg.
Effects of Wolgukwhan Methanol Extract on Oxidative Liver Injury
Moon Jin-Young ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 85~95
Objectives: In traditional medicine, Wolgukwhan has been used for the treatment of digestive system disease, such as indigestion, brash, ructation, nausea and vomiting. This study was purposed to investigate the effects of Wolgukwhan methnol extract (WGWM) on oxidative liver cell injury. Methods: In vivo assay, we administerated acetaminophen(500mg/kg, i.p.) to starved mice 24hrs after pretreatment of WGWM for 6days. In the liver homogenates, lipid peroxide and glutathione(GSH) levels were measured. In addition, activities of hepatic enzyme, such as catalase, glutathione peroxidase(GPX), glutathione S-transferase(GST) were measured in the hepatic mitochondrial and cytosolic fractions. Results: In vivo administeration of WGWM showed effective inhibition of acetaminophen induced lipid peroxidation and elevations of glutathione level. The acetaminophen treatment resulted in a decrease of catalase, GPX and GST activities. By contrast, WGWM pretreatment increased compare to those of untreated groups. Conclusions: These results suggested that WGWM might protect against lipid peroxidation by free radicals, destruction of hepatic cell membranes.
Yookmijihwang-Tang on the Plasma Corticosterone level in Mice exposed to Heat, Cold and Immobilization Stress
Kang Young-Gun ; Lee Tae-Hee ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 97~112
In order to investigate the effect of Sagoonja-Tang, Samool-Tang and Yookmijihwang-Tang against the cold, heat and immobilization stress, each formula was injected intragastically to mice exposed to forced cold water swimming(
), forced hot water swimming(
) and immobilization stress before measuring the change of plasma corticosterone level of mice. The results were as follows: 1. One hour after each Sagoonja-Tang, Samool-Tang, Yookmijihwang-Tang with the dose of 1g／kg, 3g／kg each were administered to mice, we put cold stress(
) on mice for 3min. Then those prescriptions didn't show any significant effect on plasma corticosterone level. however, Yookmijihwang-Tang(1g/kg) showed a significant increase(p〈0.05), and Samool-Tang(3g/kg) showed a small increase. 2. One hour after Samool-Tang, Sagoonja-Tang and Yookmijihwang-Tang with the dose of 1g／kg, 3g／kg each were administerd, mice exposed to heat stress(
) for 3 min. Samool-Tang(1g/kg) and Yookmijihwang-Tang(lg/kg) showed significant decrease of corticosterone level(p〈0.01, p〈0.05) but Sagoonja-Tang didn't change it. In the case of the dose of 3g/kg, only Samool-Tang decreased corticosterone level(P〈0.01), but Sagoonja-Tang, Yookmijihwang-Tang didn't make significant effect on plasma corticosterone level of the mice exposed to heat stress(
). 3. Those mice which exposed to immobilization stress one hour after Sagoonja-Tang, Samool-Tang, Yookmijihwang-Tang with the dose of 1g/kg and 3g/kg didn't show effective decrease of corticosterone level. However, Sagoonja-Tang(3g/kg), Samool-Tang(lg/kg, 3g/kg) showed the effect of mild increase of corticosterone level rather than decrease. These data revealed that the effect-variation of prescriptions depended on the kinds of stress and the dose of prescriptions had a different effect on therapy.
Studies on the antimetastasis & antiangiogenesis effects of Gamisoamsan
Yoon Sung-Chan ; Ahn Seong-Hun ; Kim Jin-Kyeoung ; Mun Yeon-Ja ; Chu Yeong-Guk ; Jeong Gyu-Yong ; Whoo Won-Hong ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 113~126
Soamsan is known as an anti-cancer remedy in the traditional Korean Medicine. To enhance the synergic effects of anti-cancer activity of Soamsan, this study reconstituted the original components of Soamsan with a slight modification and produced a novel herbal remedy, namely Gamisoamsan. Extracts of Gamisoamsan inhibited the growth of cultured CT-26 cells, mouse colon adenocarcinoma, in a dose-dependent manner
was estimated approximately
. Using tumor-bearing mouse model, in which was produced by subcutaneous injection of CT-26 cells (
cells). the effects of Gamisoamsan on tumor growth and host survival were examined by evaluating tumor volume and increase in life span. When Gamisoamsan extracts in variable doses of 100, 200 and 500mg／kg body weight per day were orally administered to tumor-bearing mice, following results were obtained: Improvement in the hematological parameters following Gamisoamsan treatment such as hemoglobin contents, red blood cells and white blood cells of the tumor-bearing mice have been observed. Gamisoamsan treatment also showed a prolongation of life span and a reduction of tumor volume in the CT-26 tumor hosts. The results of the present study suggest that Gamisoamsan extracts has a potential anti-tumor activity and may be an useful remedy to prevent and／or treat cancer.
Antioxidative and Protective Effects of Haeganjeon Extract on Oxidative Damage of Hepatocytes
Ahn Byung-Tae ; Kim Jong-Dae ; Moon Jin-Young ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 127~141
Objectives: Haeganjeon(HGJ) has been used for the treatment of liver disease in traditional medicine. The present study was carried out to evaluate the antioxidant and protective effects of HGJ extract on oxidative damage of hepatocytes by tert-butyl hydroperoxide(t-BHP). Methods: In the linoleic acid water-alcohol system, the levels of lipid peroxide(LPO) were determined by TBA method. The scavenging effect of HGJ on
(DPPH) radical was determined according to the method of Hatano. In the Fenton system(ferrous ion reaction with hydrogen peroxide), the levels of hydroxyl radical induced LPO in rat liver homogenate were determined according to the method of TBA. Inhibitory effect of HGJ on superoxide generation was measured by xanthine-xanthine oxidase system. In order to evaluate antioxidative activity of HGJ in the liver cell, cultured normal rat liver cells(Ac2F) were prepared and incubated with or without HGJ. After 18hr, cells placed in DMEM medium without serum, and then incubated with 1mM tert-butyl hydroperoxide(t-BHP) for 2hrs. Viable cells were detected by MTT assay. Conclusions: In the linoleic acid autoxidation system, HGJ extract significantly inhibited the time course of the lipid peroxidation. These effects were similar to those of BHA HGJ extracts showed about 70% scavenging effect on DPPH radical. And HGJ extract inhibited the lipid peroxide formation in rat liver homogenate induced by hydroxyl radical derived from Fenton system. In addition, HGJ extract protected the cell death induced by t-BHP and significantly increased cell viability in the normal rat liver cell. These result indicated that HGJ extract might playa protective role against oxidative hepatic cell injury by means of free radical scavenger.
Effects of Jakyakgamchotang Extract on the Trachea Smooth Muscle
Kook Yoon-Bum ; Lee Jang-Chun ; Kim Hee-Soo ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 143~158
The purpose of the present study is to determine the effect of Jakyakgamchotang on histamine or acetylcholine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats(250g, male) were killed by
exposure and a segment (4-5mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine(His) which evoked 50% of maximal response(
) was obtained from cumulative dose response curves for histamine(
). Contractions evoked by His(
) were inhibited significantly by Jakyakgamchotang. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 90.8% (p〈0.001) after
Jakyakgamchotang. In rat tracheal smooth muscle, the mean percent inhibition of acetylcholine induced contraction was 22.1% (p〈0.05) after
Jakyakgamchotang. Propranolol indomethacin and methylene blue(
) slightly but significantly attenuated the inhibitory effects of Jakyakgamchotang. These results indicate that Jakyakgamchotang can relax histamine or acetylcholine induced contraction of guinea pig and rat tracheal smooth muscle.
Effects of Daehwanggamchoeumja and its component groups on diabetes, free radical and antioxidative defense system in Alloxan-induced diabetic rats
Go Won-Do ; Gwak Dong-Gul ; Shin Hwa-Seog ; Choi Oi-Chul ; Park Sun-Dong ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 159~188
The purpose of this study was to reseach the effect of Daehwanggamchoeumja(大黃甘草飮子) and its component groups on diabetes, free radicals, and antioxidants system in Alloxan-induced diabetic rats. The experimental group was divided into three groups: Daehwanggamchoeumja(DG), and its components groups, Gamdutang (Gamcho&Daedu; DG-I) and Daehwanggamchotang(DG-2). The results were obtained as follows: 1. In the study of effect on diabetic metabolic dysfunction(Glucose, Triglyceride, Total Cholesterol, HDL Cholesterol, Total Protein, Albumin, Creatine, BUN), only DG has a significant effect. 2. In the study on free radical scavenging effect in vitro(the suppressing effect on peroxidation of linoleic acid on concentration, the scavenging effect of DPPH radical, inhibitory effect of superoxide in xanthine-xanthine oxidase system, inhibitory effect on lipid peroxidation reaction by hydroxy radical in
system, and the effect on Nitrate reductase activity), DG and DG-2 have more effect than DG-l relatively. 3. In the study on antioxidants system in vivo(The level of serum LPO, The level of hepatic LPO, Catalase, GSH, GST), only DG has a significant effect. These results suggest that Daehwanggamchoeumja(大黃甘草飮子) has an effect on diabetes, peroxidative damage by free radical, so it seems to be useful to prevent and treat diabetes. The mechanisms of these are supposed to be involved in antioxidant and three drugs' cooperative synergy effect.
Anti-Inflammatory Effect of Ethanol Extract of Angelica uchiyamana in Activated Murine RAW 264.7 macrophages
Jang Seon-Il ; Kim Hyeong-Jin ; Hwang Ki-Myeong ; Pae Hyeon-Ok ; Yun Yong-Gap ; Chung Heon-Taek ; Kim Yun-Cheol ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 189~197
The inhibitory effects of ethanol extract of Radex Angelica uchiyamana, on LPS- or IFN- plus LPS-induced production of NO, and
, and expression of iNOS and COX-2 were investigated in the activated RAW 264.7 cells. This extract significantly inhibited the production of NO and
, and suppressed the expression of iNOS and COX-2 in a dose-dependent manner. These results show that ethanol extract of Radex Angelica uchiyamana may explain some known biological activities including anti-inflammatory effect, and is of considerable benefit in the treatment for NO and pro-inflammatory cytokine overproduction related immunological diseases.
The effect of Codonopsis lanceolata on the melanogenesis
Lee Seung-Yon ; Kim Jin-Man ; Oh Hyun-Chul ; Im Sook-Jung ; Hwang Chung-Yeon ; Mun Yeun-Ja ; Woo Won-Hong ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 199~211
Acquired pigmentary skin diseases such as abnormal melanogenesis, vitiligo, chloasma and inflammatory pigmentation are related to regulate the melanin production. The aim of this study was to investigate the effect of Codonopsis lanceolata on the melanogenesis of HM3KO human melanoma cells biologically. The cells were treated for 5 days with Codonopsis lanceolata at several concentrations. Treatment with Codonopsis lanceolata suppressed melanin contents as a dose dependent manner without cytotoxicity and morphological change. And the extract of Codonopsis lanceolata also inhibited tyrosinase, a key enzyme forming melanin, in a dose-dependent manner. And it did not affect the DOPAchrome tautomerase(TRP-2) activity. These results suggest that Codonopsis lanceolata is a candidate for an efficient whitening agent which supresses melanogenesis by a Raper-Mason pathway.
Apoptosis of 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol isolated from the fruiting bodies of Isaria japonica Yasuda via Caspase-3 in Bladder cancer Cell line (NBT-II)
Kim Hyeong-Jin ; Jang S.I. ; Oh K,S. ; Hong K.H. ; Kim Y.C. ; Pae H.O. ; Yun Y.G. ; Chung H.T. ; Kwon T.O. ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 213~223
The fruiting bodies of Isaria japonica have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-Acetyl-12, 13-epoxyl -9-trichothecene-3, I5-diol (AETD), was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay -guided fractionation. The apoptosis of murine bladder cancer cell line (NBT-Ⅱ) by the compound was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity at
concentration (5 nmol／L) was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3. These results indicate that AETD induces apoptosis of NBT-Ⅱ cells via expression of caspase-3.
Inhibitory effects of Injinseulki on Chemical-induced hepatic fibrosis in Rats
Jang S.I. ; Kim H.J. ; Hwang K.M. ; Kwon T.O. ; JeKal S.J. ; Kim Y.C. ; Pae H.O. ; Choi B.M. ; Shim J.S. ; Kwak J.S. ; Chung H.T. ; Yun Y.G. ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 225~241
The inhibitory effects of Injinseulki including extracts of melanian snail plus phytomedicinal plants on
plus LPS-induced production of NO, PMA-stimulated production
, and tacrine-induced hepatic injury were investigated in rats, RAW 264.7 or HepG2 cells. The ethanol extracts of melanian snail significantly inhibited tarcrine-induced inury of HepG2 cells. Injinseulki inhibited the production of NO and
in a dose-dependent manner in activated RAW 264.7 macrophages. Injinseulki significantly inhibited chemical-induced fibrosis in Rats. These results show that Injinseulki including extracts of melanian snail plus phytomedicinal plants may explain some known biological activities of Injinseulki including their anti-inflammatory and anti-fibrotic effect, and is of considerable benefit in the treatment for live diseases.
Antioxidative effects of the fermented extract of Pinus densiflora
Mun Yeun-Ja ; Lim Nan-Young ; Lee Sung-Won ; Kang Dae-Gill ; Baik Soon-Ki ; Woo Won-Hong ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 243~249
This study was performed to investigate the effects of fermented extract of Pinus densiflora (FPD) on oxygen radicals and
-induced damage. The results are as follows: 1. The 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radicals were considerably reduced by FPD and
value was showed the concentration of 20 ㎍／㎖. 2. The cytotoxicity did not observe by FPD treatment in A548 cells. 3. The
-induced cell damage was recovered by FPD pretreatment in A549 cells. These results suggest that FPD, as a natural antioxidant, has scavenging effect of free radicals and protection effect from
Clinical Application of Shinkihuan in Hyungsang Medicine
Kim Pan-Joon ;
Herbal Formula Science, volume 10, issue 2, 2002, Pages 251~260
The application of Shinkihuan umder the combination of configuration, color, pulse and symptoms leads to the following conclusions: 1. Shinkihuan is applied to the diseases related with lung, kidney and the spine: difficult urination of old people, shortness of breath, hematuria, loss of voice, distorted vision, deafness of deficiency, hemorrhoids, thirst, carbuncle, suppurative infection, children's retardation of walking and noma, etc... 2. The effect of Shinkihuan is as follows. It nourishes the lung, replenishes the kidney fluid, relieves the phlegm, tranquilizes fever due to deficiency of blood and reinforces the liver. 3. Shinkihuan is appropriate for the persons with the following characteristics in configuration: male, tall, thin, dam-typed, inverted-triangular, prominent nosed and dry.