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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Herbal Formula Science
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Journal DOI :
The Korean Journal of Oriental Medical Prescription
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Volume & Issues
Volume 21, Issue 2 - Dec 2013
Volume 21, Issue 1 - Jun 2013
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Study on Clinical Diseases of Blood Stasis Pattern
Park, Mi Sun ; Kim, Yeong Mok ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 1~15
DOI : 10.14374/HFS.2013.21.1.001
Objectives : This article is a study on to which categories of modern diseases blood stasis patterns are assigned and the meaning of blood stasis interpreted with perspectives of Korean Medicine and modern medicine. Methods : We reviewed "Neijing", "Shanghanlun", "Yilingaicuo", "Xuezhenglun" and other books and modern clinical papers related with blood stasis. Results : 1. Blood stasis patterns are related with disorders of hemorrheology, hemodynamics, platelet function, microcirculation, microelements and endothelial damage. 2. From the types of syndrome differentiation, diverse diseases classified in type of qi deficiency with blood stasis and type of blood stasis due to qi stagnation are reported, which reflects qi and blood are closely connected. And many diseases are classified in type of kidney deficiency with blood stasis, which has something in common with chronic diseases can achieve effect from treatment considering blood stasis. 3. Diseases related with kidney involve menopausal disorder, mazoplasia, prostatitis, erectile dysfunction, chronic nephritis, renal calculus, osteoporosis and bursitis. Diseases related with heart involve coronary artery disease, arrhythmia and cerebral thrombosis. Diseases related with spleen involve gastritis, colonitis and digestive organ ulcer. Diseases related with liver involve hepatitis, hyperthyroidism and stroke. Diseases related with lung involve neurodermatitis, bronchitis and paranasal sinusitis. Conclusions : Blood stasis pattern which is one of the areas to draw medicine`s attention has broad clinical application.
Analysis of Agastache Powder to Rectify the Ki Combination for the Formula Science Common Textbook
Shin, Soon Shik ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 16~35
DOI : 10.14374/HFS.2013.21.1.016
Objectives : Proposed a formula analysis standard about the individual formula which is to be put in the specific discussions in order to increase the effect of formula education at the college of Korean medicine. Methods : Examined the ingredient combination analysis written in Agastache Powder to Rectify the Ki which was put in the 27kinds of formula science book published in both Korea and China. Results : Must analyze the formula according to the existing formula. The combination of the ingredients should be analyzed into herb pairs such as seven different combinations. The effect and disease for which medicine is efficacious, should be analyzed and tabulated by Agastache Powder to Rectify the Ki. The formula should be anlayzed and schematized by the sytem of Chief, Deputy, Assistant and Envoy. The basic formula should be analyzed and schematized by the combination of formula, adding and removing the ingredients. Analyzing Agastache Powder to Rectify the Ki into the system of Chief, Deputy, Assistant and Envoy shows the following: chief herb is Agastaches Herba; deputy herb is Perillae Folium and Angelicae dahuricae Radix; assistant herb is Pinelliae Rhizoma preparatum, Magnoliae officinalis Cortex, Citri reticulatae Pericarpium, Arecae Pericarpium, Platycodi Radix, Atractylodis macrocephalae Rhizoma, Poria, and envoy herb is Glycyrrhizae Radix preparata, Zingiberis Rhizoma recens, Jujubae Fructus. Conclusions : In conclusion, it is believed that the formula education at colleges of Korean medicine would be effectively achieved if it is processed according to the standardized formula analysis and its rule about individual formula that is to be decided.
The Effects of Lycium chinese Mill., Morus alba L. and Their Combination on the Asthmatic Murine Model
Seo, Chang-Woo ; Lee, Young Cheol ; Lee, Jang-Cheon ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 36~50
DOI : 10.14374/HFS.2013.21.1.036
Objectives : To clarify the possible effect of Lycium chinese Mill (LC)., Morus alba L (MA)., and Lycium chinese Mill. +Morus alba L. (LC+MA), we have examined their influence on the development of pulmonary eosinophilic inflammation in the asthmatic murine model. Methods : Female Balb/c mice (5weeks) were immunized on two different days (21 days and 7 days before inhalational exposure) by intraperitonial injections of 0.2ml alum-precipitated Ag containing
of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 minutes/day on 3 days/week for 8 weeks (at a flow rate of 250 L/min, 2.5% ovalbumin in normal saline) and, LC, MA, and LC+MA (500 mg/kg) were orally administered 3 times per a week for 8 weeks. Results : The suppressive effect of LC, MA, and LC+MA were demonstrated by the accumulation of eosinophills into airways, with the reduction of eosinophil, total lung leukocytes numbers. These were correlated with the marked reduction of IL-5, IL-13 and IL-4 levels in the BALF and serum. OVA-specific IgE levels were also decreased in serum and BAL from these mice. LC, MA, and LC+MA decreased eosinophil CCR3 expression and CD11b expression in lung cells. Conclusions : These results indicate that LC, MA, and LC+MA have high inhibitory effects on airway inflammation and hyper-responsiveness in the asthmatic murine model. The suppression of IL-5, IgE, eosinophil CCR3 expression and CD11b expression, and the increase of IFN-
production in BALF seem to contribute to this effect. Hence, the results indicated that LC, MA, and LC+MA could act as a immuno-modulator which possesses anti-inflammatory and anti-asthmatic property by modulating the imbalance between Th1 and Th2 cytokines.
Anticancer and Related Immunomodulatory Effects of Insamyangyoung-tang on Non-small Cell Lung Carcinoma, NCI-H520, Xenograft Mice
Song, Kwang-Kyu ; Kwak, Min-A ; Kim, Jong-Dae ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 51~70
DOI : 10.14374/HFS.2013.21.1.051
Objectives : The object of this study was to observe anticancer and related immunomodulatory effects of Insamyangyoung-tang extracts (ISYYTe) on non-small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Methods : Three different dosages of ISYYTe, 50, 100 and 200 mg/kg were orally administered once a day for 42 days from 11 days after tumor cell inoculation. Six groups, which are intact control, tumor bearing control, 5-fluorouracil (FU) 30 mg/kg, ISYYTe 50 mg/kg, ISYYTe 100 mg/kg, ISYYTe 200 mg/kg, each of 8 mice per group were used in the present study. Changes on the body weight, tumor volume and weight, lymphatic organ (spleen and popliteal lymph node), serum interferon (IFN)-
levels, splenocytes NK cell activity and peritoneal macrophage activities, splenic tumor necrosis factor (TNF)-
, interleukin (IL)-
and IL-10 contents were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. Results : As results of ISYYTe 50, 100 and 200 mg/kg treatment, decreases in the tumor volumes and weights were detected. At histopathological observations, decreases of tumor cell volumes in tumor masses were dose-dependently decreased mediated by increases of apoptosis among tumor cells by treatment of all three different dosages of ISYYTe. As results of tumor cell inoculation, marked decreases of spleen and popliteal lymph node weights, serum IFN-
, splenic TNF-
and IL-10 contents and splenocytes were observed with histopathological atrophic changes of spleen and popliteal lymph nodes. Conclusions : Over 50 mg/kg of ISYYTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory effects. Although relatively lower anticancer effects were observed in ISYYTe 200 mg/kg treated mice as compared with 5-FU 30 mg/kg treated mice, there are no meaningful favorable immunomodulatory effects were observed after 5-FU treatment in the present study.
Simultaneous Analysis of Six Compounds in Yijin-tang by HPLC-PDA
Kim, Seong-Sil ; Kim, Jung-Hoon ; Shin, Hyeun-Kyoo ; Seo, Chang-Seob ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 71~79
DOI : 10.14374/HFS.2013.21.1.071
Objectives : Yijin-tang has been used in the treatment of gastrointestinal diseases such as irritable bowel syndrome, gastritis, and gastric ulcer. In this study, a high-performance liquid chromatography (HPLC) method was established for simultaneous analysis of six compounds, liquiritin, glycyrrhizin, hesperidin, 6-gingerol, homogentisic acid, and 3,4-dihydroxybenzaldehyde in Yijin-tang, a traditional Korean herbal medicinal preparation. Methods : A Gemini C18 column was used for the separation of six constituents at
. The mobile phase using gradient elution consist of two solvent systems, 1.0% acetic acid in water (A) and 1.0% acetic acid in acetonitrile (B). The flow-rate was 1.0 mL/min and injection volume was
. The detector was a photodiode array (PDA) detector set at 254 nm and 280 nm. Results : The calibration curves of six compounds showed good linearity in various concentration ranges (
). The limits of detection (LOD) and limits of quantification (LOQ) were 0.028-
, respectively. The RSD (%) of the intra and inter day validations were 0.03-0.84% and 0.05 -1.00%, respectively. Recovery was 96.14-01.90% and RSD (%) was less than 1.5%. Conclusions : The established simultaneous analysis methods will help management to improve the quality of Yijin-tang.
Ameliorative Effects of Hwangnyeonhaedok-tang on Atopic Dermatitis
Ki, Ho-Pil ; Jang, Seon Il ; Yun, Young-Gab ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 80~90
DOI : 10.14374/HFS.2013.21.1.080
Objectives : The water extract of Hwangnyeonhaedok-tang (HHT), composed of the Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus has been traditionally used to treat fever, inflammation, gastritis and hypertension in east asia. However, little is known about the ameliorative effects of HHT on atopic dermatitis. Therefore, the purpose of this study was to investigate the therapeutic effect of HHT on atopic dermatitis Methods : We investigated the inhibitory effects of HHT on the production of proinflammatory cytokines in rat peritoneal mast cells (RPMCs), on the scratching behavior in ICR mice, and on atopic dermatitis symptoms in 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis-like model hairless mice. Results : Levels of TNF-
increased by PMA plus A23187 co-treatment were significantly inhibited by HHT in a dose-dependent manner. HHT also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. The oral administration of HHT reduced the scratching behavior induced by compound 48/80 and histamine in ICR mice. Furthermore, the intradermal treatment of HHT reduced the ear edema, skin lesions, and atopic molecular marker (IgE and IL-4) in DNFB-induced atopic dermatitis model mice. Conclusions : These results suggest that HHT may be used as a potential treatment for AD as a prescription for treatment of atopic dermatitis.
Skin Whitening Effects of Extracts from Angelicae Gigantis Radix and Lycii fructus Ethanol Extracts
Hwang, Sung Yeoun ; Lee, Jeong Tak ; Kim, Yeong Uk ; Kim, Hong Jun ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 91~98
DOI : 10.14374/HFS.2013.21.1.091
Objective : The purpose of this study was to investigate the synergistic effect of Angelicae gigantis Radix (AG) and Lycii fructus (LF) ethanol extracts on skin-whitening effects. Method : LFAG extracts were prepared by extracting with 80% ethanol. The efficacy of LFAG was judged by measurement of cell viability, tyrosinase activity, melanin production, tyrosinase and microphthalmia-associated transcription factor (MITF) expression in B16F10 murine melanoma cells by lipopolysaccharides (LPS) treatment. Results : Each extract (LF or AG) inhibited the tyrosinase activity in a dose-dependent manner. The co-treatment of LFAG extracts (
AG) markedly suppressed the LPS-induced cellular tyrosine activity, melanin production, tyrosinase and MMP-1 expression in B16F10 murine melanoma cells. These suppressive effects were synergistically increased by their combination. Conclusions : With these observations, we suggest that the extracts from Lycii fructus and Angelica gigantis Radix could be potent natural materials for whitening skin.
Effects of a Herbal Preparation HJ01 on Adipocyte Differentiation in OP9 Cells and the Poloxamer-407 Induced Hyperlipidemia in Mice
Park, Jung-Eun ; Han, Sang-Yong ; Choi, Eun-Sik ; Chong, Myong-Soo ; Kim, Yun-Kyung ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 99~110
DOI : 10.14374/HFS.2013.21.1.099
Objectives : This study was designed to investigate the effect of a herbal preparation HJ01 consisting of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus and Glycyrrhizae Radix on adipocyte differentiation in OP9 cells and on poloxamer 407(P-407)-induced hyperlipidemia in mice. Methods : 1. MTT assay was used to evaluate the potential cytotoxicity of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01, respectively. 2. Bone-marrow derived OP9 cells were treated with HJ01, and the alterations in fat storage in the cells were determined by the Oil red O assay. 3. The protein level of CAAAT/enhancer binding protein alpha(
), as a adipocyte differentiation marker, was examined using western blot analysis in differentiated OP6 cells. 4. Adult male C57BL6 mice received intraperitoneal injections of P407 to induce hyperlipidemia, simultaneously, were treated with HJ01 for 4 weeks. Then the cholesterol (TC), triglyceride (TG) and high-density lipoprotein-cholesterol (HDL-c) levels in sera and liver tissues were measured. Results : 1. The MTT assay exhibited that Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01 showed no significant cytotoxicity in tested dosages. 2. Ten days` treatment with HJ01 markedly inhibited the increases in fat storage in differentiated OP6 cells. 3. Four weeks` treatment with HJ01 down-regulated the protein level of CAAAT/enhancer binding protein alpha(
) but up-regulated the levels of adiponectin in differentiated OP9 cells. 5. HJ01 inhibited the accumulation of TC and TG in liver tissues and increased serum levels of TC in hyperlipidemic mice. Conclusions : These results suggest that HJ01 can in vitro inhibit adipocyte differentiation and fat storage in OP6 cells, in vivo improve the hyperlipidemia induced by P-407 in mice, which may be mediated by promoting glucose uptake and improving a lipid metabolite profile.
Acute Toxicity Study on Oryeong-san in Mice
Park, Hwayong ; Hwang, Youn-Hwan ; Ha, Jeong-Ho ; Jung, Kiyoun ; Ma, Jin Yeul ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 111~118
DOI : 10.14374/HFS.2013.21.1.111
Objectives : Traditional medicine Oryeong-san (ORS) has been prescribed for a long time to treat light fever, thirst, dysuria, and accompanying edema. However, the acute toxicity and safety were not reported. In this study, we evaluated the potent acute toxicity and safety of ORS. Methods : ICR mice were used to evaluate acute toxicity and safety by oral administration of 0, 500, 1,000, 2,000mg/kg of ORS. Mortality, body weight, and clinical symptoms were observed, and organ weight and blood biochemical parameters were analyzed after necropsy. Results : We found no mortality and no toxic or abnormal clinical symptoms by administration of ORS. Comparing with control group, no significant alterations in organ weight and blood biochemical parameters were observed. Conclusions : ORS recognized as safe and non-toxic medicinal material, and median lethal dose considered to be over 2,000 mg/kg in both male and female ICR mice.
Therapeutic Effect of Doinseunggi-tang on Diabetic Vascular Dysfunction
Lee, Yun Jung ; Kim, Eon Kuk ; Kim, Hye Yoom ; Yoon, Jung Joo ; Lee, So Min ; Lee, Yong-Pyo ; Lee, Geon Mok ; Kang, Dae Gill ; Lee, Ho Sub ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 119~130
DOI : 10.14374/HFS.2013.21.1.119
Objectives : Diabetes mellitus is the leading cause for vascular complications such as atherosclerosis. The present study is to investigate whether Doinseunggi-tang (DST) improves diabetic vascular dysfunction in type II diabetes. Methods : The db/db mice were treated with high fat/high cholesterol diet and DST (200 mg/kg/day) for 8 weeks. Results : DST significantly lowered blood glucose and systolic blood pressure. In addition, DST also markedly decreased total plasma cholesterol, triglyceride, and LDL-cholesterol, whereas increased the HDL-cholesterol. Vascular relaxation of aortic rings by acetylcholine or SNP was ameliorated by DST in a dose-dependent manner. Damage of vascular intima and hypertrophic of media was improved by DST. Immunohistological study revealed that DST attenuated the increase of ICAM-1, VCAM-1, and ET-1 expression in thoracic aorta. Conclusions : Taken together, DST suppressed hyperglycemia and diabetic vascular dysfunction in type II db/db mice. The present data suggests that Doinseunggi-tang may be prevent a development of diabetic atherosclerosis.
The Ameliorative Effects of Gagam-GongJin-dan (WSY-1075) in Contact Dermatitis-induced Animal Model
Hwang, Sung Yeoun ; Lee, Chia Wei ; Lee, Seung Ho ; Kim, Hong Jun ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 131~141
DOI : 10.14374/HFS.2013.21.1.131
Objectives : This study was performed to assess the ameliorative effects of Gagam-GongJin-dan (WSY-1075) composited with Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Ginseng Radix, Cervi parvum Cornu and Cinnamomi Cortex in contact dermatitis animal model. Methods : WSY-1075 was orally administrated the various concentrations (50-400 mg/kg, body weight/day) with one time per day for 10 days from 4 days after DNFB sensitization. We investigated ameliorative effects of WST-1075 on the scratching behavior, skin clinical serverity and inflammatory mediators in 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis mice. Results : The orally administration of WSY-1075 (400 mg/kg) inhibited the scratching behavior induced by sensitization and challenge with DNFB. WSY-1075 (200 mg/kg or 400 mg/kg) administration also reduced the skin damage, inflammatory mediators, mast cell infiltration induced by DNFB. Moreover, WSY-1075 (above 200 mg/kg) administration inhibited the serum levels of IgE and IL-4 in DNFB-induced contact dermatitis mice. Conclusions : These results suggest that WSY-1075 administration (200 mg/kg or 400 mg/kg) has the ameliorative effects on the scratching behavior, the clinical signs, mast cell infiltration and inflammatory mediators in DNFB-induced contact dermatitis animal model mice.
The Editing System and Characteristics of "Clinical Formula Science of Korean Medicine"
Shin, Soon Shik ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 142~153
DOI : 10.14374/HFS.2013.21.1.142
Objectives : Examined the editing system and the characteristics of "Clinical Formula Science of Korean Medicine" to establish a basis for compilation system of the common formula science textbook for colleges of Korean medicine nationwide. Methods : Comprehended the "Clinical Formula Science of Korean Medicine" published by professor Hyeongok(1911-1987) in 1985 and analyzed its configuration system. Results : "Clinical Formula Science of Korean Medicine" has made it possible to understand the formula systematically by establishing and classifying 24 kinds of treatment method [the three treatment methods of cold-dampness, dryness-heat and interior deficiency and its subdivision, 19 kinds of treatment method (stomach, phlegm, eum, yang, spirit, essence, ki, blood, blood stasis, exterior, interior, half-exterior half-interior, water, wind, cold, heat, dampness) combined with reducing and vomiting] and main formula. These can be considered as professor Hyeongok`s own method of classification, which is distinctive from the existing formula science system. Also, it suggests that a Korean medicine doctor should not use a biased formula science that is limited to some schools but apply the formula widely and synthetically because it is believed to pursue perfection in cure if the main formulas related to a physical disorder, pregnancy and childbirth, invigorating and purging five viscera, and Four-Constitution Medicine are added. Conclusions : "Clinical Formula Science of Korean Medicine" is seen as a formula science book which is to be consulted when compiling a common formula science textbook for colleges of Korean medicine by forming a Korean formula science system that is distinctive from that of China.
Inhibitory Effect of Rhein on Renal Fibrosis in Diabetic Nephropathy Rats
Zhao, Rongjie ; Zhao, Zhenglin ; Zhang, Jie ; Liu, Hongfeng ; Cui, Rongjun ; Kim, Sang Chan ; Kim, Sun-Hyung ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 154~160
DOI : 10.14374/HFS.2013.21.1.154
Objectives : To investigate the therapeutic effect and underlying mechanisms of rhein on renal fibrosis in diabetic rats. Methods : Diabetic nephropathy (DN) was induced in adult Wistar rats via introperitoneal injection of streptozotocin (STZ) (20 mg/kg/d) for three consecutive days. Two days after the last dose of STZ, rhein was administered to the diabetic rats at a dose of 25 mg/kg or 50 mg/kg, twice a day by gavage, respectively. Following 28 days treatment with rhein, the plasma glucose and creatinine levels were measured, the renal levels of TGF-
protein and mRNA were examined, and the fibronectin mRNA levels were also determined. Results : Rhein significantly inhibited the increased plasma glucose and creatinine levels of diabetic rats in a dose- and a time-dependent way. Immunohistochemical analysis showed both doses of rhein markedly attenuated elevated induction of renal TGF-
protein expressions in diabetic rats. Additionally, the high dose of rhein improved both TGF-
and fibronectin mRNA expressions, while the low dose of rhein only alleviated fibronectin mRNA expressions. Conclusions : Rhein can improve renal fibrosis in diabetic nephropathy rats, and which may be mediated through inhibition of the renal mRNA expressions of TGF-
Inhibitory Effect of a Decoction Combined with Ostericum koreanum Maxim. and Aralia continentalis Kitagawa on Collagen II-induced Arthritis Mice
Yoon, Ho-Suk ; Lee, Young Cheol ; Lee, Jang-Cheon ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 161~176
DOI : 10.14374/HFS.2013.21.1.161
Objectives : To clarify the anti-arthritic activity of Ostericum koreanum Maxim. (OS) plus Aralia continentalis Kitagawa (AC) in vivo. Methods : All mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with OS plus AC once a day for 7 weeks. Oral administration of OS plus AC (200 or 50 mg/kg) significantly suppressed the progression of CIA, which extend is comparable to that of methotrexate (MTX, 0.3 mg/kg), a positive control. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. Results : Administration of OS plus AC significantly suppressed the progression of CIA and inhibited the production of TNF-
and IL-6 in serum. The erosion of cartilage was dramatically reduced in mouse knees after treatment with OS plus AC. In conclusion, our results demonstrates that OS plus AC significantly suppressed the progression of CIA and that this action was characterized by the decreased production of IL-6, IFN-
and collagen II specific antibody in serum, CD3+CD69+ T cells, MHC class II+/CD11c+ (in DLN), CD11b+Gr-1+ cells (in PBMC), CD11b +Gr-1+ cells, B220+/CD23+ (in paw joint). Conclusions : The the levels of IFN-
in the culture supernatant of splenocytes stimulated with CD3/CD28 or collagen were dramatically decreased, while those of IL-4 was increased. In the serum of OS and AC-treated mice, the levels of IgM RA factor were decreased.
Quantification Analysis and Antioxidant Activity of Leejung-tang
Seo, Chang-Seob ; Kim, Ohn Soon ; Kim, Yeji ; Shin, Hyeun-Kyoo ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 177~185
DOI : 10.14374/HFS.2013.21.1.177
Objectives : Leejung-tang (Lizhong-tang) has been used for treatment of gastrointestinal disorders in Korea. In this study, we performed quantification analysis of five marker components, liquiritin, ginsenoside Rb1, ginsenoside Rg1, glycyrrhizin, and 6-gingerol in Leejung-tang using a ultra performance liquid chromatography- electrospray ionization-mass spectrometer (UPLC-ESI-MS). In addition, we evaluated antioxidant activity of Leejung- tang. Methods : The column for separation of five constituents used a UPLC BEH C18 (
) maintained at
. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in H2O (A) and CH3CN (B) by gradient flow. The flow rate was 0.3 mL/min with detection at mass spectrometer. The antioxidative activities conduct an experiment on 2,2`-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of Leejung-tang. Results : Calibration curves of five marker compounds were acquired with r2 values > 0.99. The amount of the five compounds in Leejung-tang were 0.07 - 0.84 mg/g. The concentration required for 50% reduction (RC50) against ABTS radical was 119.02 ug/mL. In addition, the scavenging against DPPH radical of Leejung-tang was 11.4%, 14.5%, 19.8%, 29.6%, and 49.2% at 25 ug/mL,
, respectively. Conclusions : The established LC-MS/MS method will be helpful to improve quality control of Leejung-tang. In addition, Leejung-tang is a potential antioxidant therapeutic agent.
Mouse Single Oral Dose Toxicity Test of Red Koji Fermented Scutellariae Radix Aqueous Extracts
Park, Jae-Chan ; Choi, Hae-Yun ; Kim, Jong-Dae ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 186~199
DOI : 10.14374/HFS.2013.21.1.186
Objectives : The objectives of this study was to obtain acute information (single oral dose toxicity) of Red-Koji (Monascus purpureus 12002) Fermented Scutellariae Radix Aqueous Extracts (fSR), has been traditionally used in Korean medicine for treating various diseases including inflammatory diseases. Methods : In order to observe the 50% lethal dose (LD50), approximate lethal dosage (ALD) and target organs, fSR powders were once orally administered to female and male ICR mice at dose levels of 2,000, 1,000, 500 and 0 (control) mg/kg (body weight.). The mortality and changes on body weight, clinical signs and gross observation were monitored during 14days after single oral treatment of fSR with organ weights and histopathological observations of 12 types of principle organs. Results : After single oral treatment of fSR, we could not find any mortality and toxicological evidences up to 2,000 mg/kg treated group, the limited dosages in rodents, on the body and organ weights, clinical signs, gross and histopathological observations, except for some accidental findings. Conclusions : The results obtained in this study suggest that the LD50 and ALD of fSR in both female and male mice after single oral treatment were considered as over 2,000 mg/kg because no mortalities were detected up to 2,000 mg/kg and can be safety used in clinics.
A Case of Nummular Eczema Treated with Bokryeongkamcho-tang and Yeongkyechulkam-tang
Jeong, Jong-Kil ; Lee, Soong-In ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 200~205
DOI : 10.14374/HFS.2013.21.1.200
Objectives : The purpose of this study is to report the clinical effectiveness of herbal remedies (Bokryeongkamcho -tang. Yeongkyechulkam-tang.) on nummular eczema. Methods : The 49 year-old male patient has been suffered from nummular eczema for 8 months. At first, he was treated with Bokryeongkamcho-tang for 56 days. And then he was treated with Yeongkyechulkam-tang for last 40 days. During the treatment we checked pruritis degree changes and extent changes by asking. And we checked how prulent exudates, crusts, scratch marks are changes by taking photos. First evaluation was performed before treatment, second evaluation was performed after taking Bokryeongkamcho-tang 56 days, third evaluation was performed after taking Yeongkyechulkam-tang 40 days. Results : After taking Bokryeongkamcho-tang 56 days, pruritus was decreased and prulent exudents were stopped, and crusts were disappeared. Nummular eczema on trunk, arm, thigh was disappeared. But light pruritis was still going on, and small circle-shaped vesicles groups were lagged on forearm and shin, ankle. So we found other symptoms from the patient, and administered Yeongkyechulkam-tang. After taking Yeongkyechulkam-tang 40 days, pruritus was almost decreased and nummular eczema on fore arm, shin was disappeared. Conclusions : This clinical case indicates that herbal remedies(Bokryeongkamcho-tang. Yeongkyechulkam-tang.) are effective on nummular eczema.
A Case Report of Tension Type Headache Patient with Dangkisoo-san and Acupuncture
Yun, Yong-Jae ; Kim, Koang-Lok ; Jin, Myung-Ho ; Kang, You-Gyung ; Nam, Woo-Jin ; Park, Dong-Il ;
Herbal Formula Science, volume 21, issue 1, 2013, Pages 206~212
DOI : 10.14374/HFS.2013.21.1.206
Objectives : The purpose of this study is to report the clinical effects of Dangkisoo-san and acupuncture on a patient with Tension Type headache. Methods : We have given Dangkisoo-san and acupuncture treatment to a patient with Tension Type headache for 8days, and checked changes of symptoms. Main symptoms were numbness and pains in a head and a neck. Results : After treatment, the numbness and pains in a head and a neck were improved considerably. Conclusions : This study shows that Dangkisoo-san and acupuncture are significantly effective in treatment of Tension Type headache.