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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Herbal Formula Science
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Journal DOI :
The Korean Journal of Oriental Medical Prescription
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Volume & Issues
Volume 23, Issue 2 - Dec 2015
Volume 23, Issue 1 - Jun 2015
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Effects of Sagan-tang and individual herbs on COPD Mice Model
Han, Jong-Min ; Yang, Won-Kyung ; Kim, Seung-Hyeong ; Park, Yang-Chun ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 171~187
DOI : 10.14374/HFS.2015.23.2.171
Objective This study aimed to evaluate the effects of Sagan-tang (SGT) on COPD mouse model. Methods The study was carried out by two ways (in vitro, in vivo). In vitro RAW264.7 cells (mouse macrophage) were used and analysed by flow cytometry, ELISA, Western blot. In vivo LPS and CSS challenged mice were used and its BALF had been analysed by cytospin image, FACS, ELISA, lung tissue by real-time PCR. Results In vitro, SGT maintained 80-100% rate of viablilty on 10 ~ 500 ㎍/㎖ concentration. In ELISA analysis with RAW264.7 cells, SGT significantly decreased NO over 30 ㎍/㎖. In flow cytometry, SGT 100 ㎍/㎖ dosage group displayed a tendency for decrease ROS. In Western blot analysis, SGT 100 ㎍/㎖ dosage group decreased NF-κB. In ELISA analysis, SGT significantly decreased TNF-α, IL-6 over 200 ㎍/㎖. In vivo SGT 200 ㎎/㎏ dosage group, application of SGT significantly decreased increase of neutrophils, TNF-α, IL-6 in BALF, muc5AC, TGF-β, TNF-α, expression of mRNA in lung tissue and histological lung injury. Conclusion This Study suggests usability of SGT for COPD patients by controlling lung tissue injury.
Mitochondria protection of Sparganii Rhizoma against oxidative stress in heptocytes
Seo, Hye-Lim ; Lee, Ju-Hee ; Jang, Mi-Hee ; Kwon, Young-Won ; Cho, Il-Je ; Kim, Kwang-Joong ; Park, Sook-Jahr ; Kim, Sang-Chan ; Kim, Young-Woo ; Byun, Sung-Hui ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 189~198
DOI : 10.14374/HFS.2015.23.2.189
Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte. Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line. Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK. Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.
Induction of Cell Cycle Arrest at G2/M phase by Ethanol Extract of Scutellaria baicalensis in Human Renal Cell Carcinoma Caki-1 Cells
Park, Dong-Il ; Jeong, Jin-Woo ; Park, Cheol ; Hong, Su-Hyun ; Shin, Soon-Shik ; Choi, Sung-Hyun ; Choi, Yung-Hyun ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 199~208
DOI : 10.14374/HFS.2015.23.2.199
Objectives : In the present study, we investigated the effects of ethanol extract of Scutellaria baicalensis (EESB) on the progression of cell cycle in human renal cell carcinoma Caki-1 cells. Methods : The effects of EESB on cell growth and apoptosis induction were evaluated by trypan blue dye exclusion assay and flow cytometry, respectively. The mRNA and protein levels were determined by Western blot analysis and reverse transcription-polymerase chain reaction, respectively. Results : It was found that EESB treatment on Caki-1 cells resulted in a dose-dependent inhibition of cell growth and induced apoptotic cell death as detected by Annexin V-FITC staining. The flow cytometric analysis indicated that EESB resulted in G2/M arrest in cell cycle progression which was associated with the down-regulation of cyclin A expression. Our results also revealed that treatment with EESB increased the mRNA and proteins expression of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1), without any noticeable changes in cyclin B1, Cdk2 and Cdc2. In addition, the incubation of cells with EESB resulted in a significant increase in the binding of p21 and Cdk2 and Cdc2. These findings suggest that EESB-induced G2/M arrest and apoptosis in Caki-1 cells is mediated through the p53-mediated upregulation of Cdk inhibitor p21. Conclusions : Taken together, these findings suggest that EESB may be a potential chemotherapeutic agent and further studies will be needed to identify the biological active compounds that confer the anti-cancer activity of S. baicalensis.
Building the Database with Herbal Formulas Based on the Korean Medical Classics
Kim, Ji-Hoon ; Lee, Dae-Jung ; Yun, Sung-Joong ; Ahn, Sang-Woo ; Kim, Yun-Kyung ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 209~224
DOI : 10.14374/HFS.2015.23.2.209
Objective : The main purpose of this study is to find a reasonable solutions for utilization of herbal formulas based on the medical classics. Methods : We used official Ten `Selected Korean Medical Classics(SKMC)`. For input of formulas from SKMC, we had to select proper editions of them. After consultations with experts of `The Korean Medicine Society for the Herbal Formula Study` and `Korean Society of Medical History`, we chose the proper editions of SKMC, and according to those books, we built formulas database using microsoft office excel sheets, respectively. Results : After whole input procedures, we requested several experts to inspect the results and also, to correct errors in them. Input categories were name of formula, name of classic, classification, original source, composition herb, weight, unit of weight, weight conversion index, converted weight, basic formula, indications, reason for modification, preparing method, taking method and contraindications. Final number of whole formulas in DB was 32,882. Conclusions : We used SKMC and built database using excel program. Through this procedure, we can provide a developmental ways to make a foundation for herbal formulas from the medical classics, so that we can make herbal formulas be standardized or industrialized more efficiently.
Consideration in the Interpretation of the Pyeongwi-san Prescription
Choe, Ung-Sik ; Lee, Won-Yung ; Jeong, Gi-Hoon ; Seo, Young-Bae ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 225~233
DOI : 10.14374/HFS.2015.23.2.225
Objectives : The purpose of this study was to investigate the interpretation of the Pyeongwi-san(PWS) prescription in order to obtain the evidence for clinical applications. Methods and Result : We interpreted on the PWS-related contents based on 15 classic books, analyzed it according to followed categories ; origin, indication and precaution, usage, meaning of name, physiological and pathological situation of PWS, explain about each herbs. Conclusion : 1. PWS was first mentioned in the Bakjebang, its application was enhancing appetite. Application of PWS not only expanded digestive disease, infectious disease, and pain in articular, but also used to toniyfing drug. 2. Generous application method of PWS was ‘Sujunbok(水煎服)’. Pill preparation(丸劑) and decoction with salt(鹽湯劑) were also used 3. "Pyeongwi(平胃)“ in PWS means "flatten the stomach". There are some theory about methodology to "Pyeongwi (平胃)“, one is "drain the pathogen in stomach(敦阜說)”, other is "tonifying stomach`s healthy qi(卑監說)“, the other is compromise theory. 4. Most medical literature related application situation of PWS to dampness(濕邪) in stomach. In Gangsulwongobangsunju, there are more detailed explain focused on metal qi(金氣), and fire qi(火氣) 5. Atractylodes japonica Koidz(蒼朮) drys dampness and fortifys the spleen, Magnolia officinalis Rehder(厚朴) eliminates dampness and treats fullness, Citrus reticulata Blanco(陳皮) moves and drains qi, Glycyrrhiza uralensis Fisch(甘草) tonify and harmonize in herbal formula
Study on the "Moschus substitute for Aquilariae Resinatum Lignum or Aucklandiae Radix" of Gongjin-Dan In The Classic Literature.
Lee, Min-Seok ; Lee, Jong-Hoon ; Yoon, Tae-Kwan ; Lee, Jang-Cheon ; Lee, Boo-Kyun ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 235~243
DOI : 10.14374/HFS.2015.23.2.235
Objectives : The object of this paper is to seek and provide evidences for the possibilities of substitutional use of Aquilariae Resinatum Lignum and Aucklandiae Radix for Moschus as an ingredient of Gongjin-dan by clarifying the similarities and differences among these herbs in the classic literatures. Methods : We have found out the features of Moschus, Aquilariae Resinatum Lignum and Aucklandiae Radix by searching Qi, Flavor and Effect of each herb in 『Shennongbencaojing』, 『Zhengleibencao』, 『Bencaogangmu』, 『Dongeuibogam』, 『Bencaobeiyao』, 『Euijongsonik』 and 『Bangyakhappyeon』. And we have compared and analyzed the fomula of Gongjin-dan and other prescriptions in 『Beijiqianjinyaofang』, 『Shengjizonglu』, 『Shiyidexiaofang』, 『Dongeuibogam』, 『Euijongsonik』, 『Euibanghwaltu』 and 『Bangyakhappyeon』. Results : We could find out the similarities and differences in Qi, Flavor and Effect among Moschus, Aquilariae Resinatum Lignum and Aucklandiae Radix. And we could also find the examples of substitutional use of Aquilariae Resinatum Lignum as an ingredient of Gongjin-dan by comparing the formula of Gongjin-dan in the classic literatures as above. And in the other prescription, Aucklandiae Radix was also used instead of Moschus. Conclusions : All of three herbs have pungent in Flavor and warm in Qi. And these herbs also have similar effects in dispersing invading pathogenic factors and smoothening the flow of Qi. And according to 『Bencaobeiyao』, 『Euijongsonik』 and 『Bangyakhappyeon』, Aquilariae Resinatum Lignum and Aucklandiae Radix direct and regulate the Qi in common. The substitutional use of Aquilariae Resinatum Lignum and Aucklandiae Radix instead of Moschus in Gonjin-dan seems to be related to `the ascent of Water and descent of Fire` effect of Gongjin-dan.
Clinical Use of Prescriptions Including Coptidis Rhizoma-Evodiae Fructus Pair
Joh, Hae-In ; Kook, Yoon-Bum ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 245~259
DOI : 10.14374/HFS.2015.23.2.245
Objectives : This study is intended to establish the areas of use of the major effects of Coptidis Rhizoma-Evodiae Fructus Pair in the Prescriptions. Methods : Prescriptions that include both Coptidis Rhizoma and Evodiae Fructus have been selected with the use of programs offered by
. This study compared and analyzed the main effects of the selected prescriptions. Results : Prescriptions that work on upper digestive system tend to have Coptidis Rhizoma to Evodiae Fructus ratio of 2:1~10:1, and Prescriptions that work on lower digestive system tend to have ratio of 1:1. When Coptidis Rhizoma takes up 50% or more of the total dose of the prescriptions, these prescriptions tend to have major effect on the upper digestive system, and when it takes less than 50%, these tend to have major effect on the lower digestive system on the contrary. Conclusions : The prescriptions based on Coptidis Rhizoma-Evodiae Fructus Pair have a tendency to treat digestive problems caused by mass of fever in Liver-system. On this basis, further studies are expected to unveil the effectiveness of these prescriptions on adult diseases.
Case Report of Post-CVA Voiding Dysfunction Treated with Gamidaejihwang-tangaggam
Hong, Sang-Hoon ; Park, Sang-Eun ;
Herbal Formula Science, volume 23, issue 2, 2015, Pages 261~269
DOI : 10.14374/HFS.2015.23.2.261
Objectives : The purpose of this case reports is to report the possibility of Post-CVA voiding dysfunction treated with Gamidaejihwang-tangaggam(加味大地黃湯加減). Methods : Three patients with Post-CVA voiding dysfunction were treated by Gamidaejihwang-tanggagam. Effect of treatment is assessed as Bladder Function Parity. Results and conclusions : Bladder function parity score improved, in three case. Additional research is needed on the effects of Gamidaejihwang-tanggagam for a Post-CVA Voiding disfunction.