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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
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Volume & Issues
Volume 1, Issue 4 - Dec 2006
Volume 1, Issue 3 - Sep 2006
Volume 1, Issue 2 - Jun 2006
Volume 1, Issue 1 - Mar 2006
Selecting the target year
Anti-inflammatory Compounds from New Zealand Marine Organisms
Webb, Victoria L. ; Pearce, A. Norrie ; Maas, Elizabeth W. ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 137~143
The market for anti-inflammatory drugs is large and is expanding rapidly as populations age. Key to the development of new drugs are novel chemotypes. Marine organisms harbour a diverse range of unique compounds with applications in a multitude of disease indications. This review looks at anti-inflammatory compounds isolated from New Zealand marine organisms.
Bioactive Constituents of Marine Sponges of the Genus Spongosorites
Bao, Baoquan ; Hong, Jongki ; Lee, Chong-O. ; Cho, Hee Young ; Jung, Jee H. ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 144~155
This report reviews the literatures on chemical constituents of marine sponges of the genus Spongosorites and also highlights our own research. Specific biological activities of the metabolites from these sponges include: cytotoxic, antitumor, antibacterial, antifungal, antiviral, anti-inflammatory, and other pharmacological activities.
Anti-angiogenic, Anti-cell Adhesion Switch from Halophilic Enterobacteria
Lim, Jong Kwon ; Seo, Hyo Jin ; Kim, Eun Ok ; Meydani, Mohsen ; Kim, Jong Deog ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 156~162
The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of
) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited non-activated U937 monocytic cell adhesion to HUVECs activated with IL-
by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with IL-
to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to IL-
stimulated HUVECs was completely suppressed by 121% at a concentration of 18.5 ug/mL. PLM-f74 blocked signal pathways from VEGFR2, PI3K,
-catenin and VE-cadherin to NF-kB based on western bolt analysis. And also inhibited IL-1-stimulated HUVEC expression of the adhesion molecules, ICAM-1 by 40%, VCAM-1 by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities and non-toxicity may be expected from these results.
Genomic Characterization and Pathogenicity of Iridovirus Isolated from Pearl Gourami (Trichogaster leeri)
Kim, Ho Yeoul ; Jeong, Joon Bum ; Jun, Lyu Jin ; Yoon, So Hye ; Cho, Hye Jin ; Jeong, Hyun Do ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 163~169
In the survey of the samples infected by iridovirus, we found the natural outbreak of iridovirus disease in the ornamental fish, pearl gourami (Trichogaster leeri) in Korea. It was characterized by the appearance of enlarged cells and necrosis in the observation of the imprinted spleen cells under iridovirus. To determine the infection of iridovirus more accurately, PCR using 2 different primer sets for MCP and ATPase gene used for the diagnosis of iridovirus infection was done and found the produced fragments matched with the size of expectation. Partially determined nucleotide sequences of the MCP gene of the iridovirus isolated in this study showed very high homology (99.6%) with that of ISKNV as a reference strain. In challenge experiment with the iridovirus isolated from the moribund of pearl gourami, the pathogenicity of the isolated iridovirus was confirmed and suggested the potential of the risk associated the transfer of iridovirus from the ornamental fishes to the marine culturing fishes.
Evaluation of DPPH Radical Scavenging Activity of Jeju Seaweeds Using High Throughput Screening (HTS) Technique
Kim, Kil-Nam ; Heo, Soo-Jin ; Cha, Seon-Heui ; Jeon, You-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 170~177
As a rapid and quick bioactive compound evaluation technique, in this study we utilized a automatic system of High Throughput Screening (HTS) to investigate DPPH radical scavenging efficacy of seaweeds, collected from Jeju Island in Korea. In this study, 6 species of green seaweeds, 18 species of brown seaweeds and 22 species of red seaweeds extracted with methanol at
were subjected to HTS. Of the green seaweeds tested, Enteromorpha compressa (20G6) of the green seaweeds showed DPPH radical scavenging activity of over 60%. On the other hand, the other green seaweeds showed poor DPPH radical scavenging activities (lees than 40%) at 1 mg/ml. Sargassum siliquastrum (20B17, 70B17), Dictyota dichotoma (70B1), Sargassum coreanum (70B16) and Ecklonia cava (70B26) among the brown seaweeds showed significantly high DPPH radical scavenging activity with 96%, 97%, 92%, 92%, 87%. Polysiphonia japonica (20R24), Schizymenia dubyi (20R17), Gelidium amansii (20R18) and Acrosorium flabellatum (20R23) among the red seaweeds showed remarkable DPPH radical scavenging activity of over 90%.
Antioxidant Effect of Mul-kimchi on Hepatic Tissue of Rats Fed with High Cholesterol Diet
Beik, Kyung-Yeun ; Kim, Mee-Jung ; Kim, So-Ja ; Yang, Jae-Ho ; Kim, Soon-Dong ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 178~185
This study was to investigate the effect of 0.1% chitosan-ascorbate (CA) prepared with different molecular weight (223, 746, 1,110 and 2,025 kDa) of chitosan on the changes in antioxidant activity of mul-kimchi during storage at
for 20 days. Animal experiments were divided to 5 groups; normal control group (NC), high cholesterol diet group (HC), high cholesterol diet mul-kimchi diet group (HCKC), high cholesterol diet and CA2025 containing mul-kimchi administrated group (HCCA), and high cholesterol diet and 1/2 concentrated CA containing mul-kimchi administrated group (HC2CA). Mul-kimchi juice was administered 0.5 mL per 100 g body weight once a day and fed for 5 weeks. Electron donating activity of the 20 days-stored mul-kimchi with 0.1% CA showed higher activity (84.74~89.13%) than those of control and ascorbic acid mul-kimchi (35.04 and 75.04%). Superoxide dismutase activities of the kimchijuice with CA were higher in the higher molecular of chitosan. In the animal experiments, the average body weight of the HCCA and HC2CA group were lower 6.9% and 8.4% than that of HC control group, respectively. Hepatic glutathione content in HCCA and HC2CA group was increased 22.5% and 9.1% as compared to HC group. Hepatic glutathione S-transferase activities were significantly increased in the HCCA (219.9%) and HC2CA group (153.8%) compared to NC group. Hepatic superoxide dismutase activity was highest in the HCCA group, and the activities in CA groups were higher than those of NC and HC group.
Two Novel Families of Short Interspersed Repetitive Elements from the Mud Loach (Misgurnus mizolepis)
Lim, Hak-Seob ; Kim, Moo-Sang ; Kim, Ok-Soon ; Kim, Ji-Yeon ; Choi, Young-Mi ; Ahn, Sang Jung ; Lee, Hyung-Ho ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 186~192
Short interspersed repetitive elements (SINEs) are dispersed throughout eukaryotic genomes. These SINEs have been shown to be excellent phylogenetic markers for the closed related species. In this report, we isolated two novel families of SINEs from the mud loach. The two SINE families, mlSINE-L and mlSINE-S, have genomic lengths of about 410bp and 270bp, respectively. 5' and 3' ends of the SINE families are well conserved and highly homologous to each of corresponding ends of RSg-1 and SmaI SINEs. Phylogenetic analysis shows that mlSINEs are unique to the mud loach. A dot blot hybridization experiment shows that mlSINE-L has an estimated copy number of
(2.8 pg) and is more frequently distributed at nuclear matrix attachment regions (MARs) than loop DNAs. The result suggests that mlSINEs may preferentially integrate in or near MARs.
Effect of Asterina pectinifera on Activities of Breast Cancer Chemopreventive and Metastatic Enzymes
Nam, Kyung-Soo ; Kim, Mee-Kyung ; Cho, Hyun-Jung ; Shon, Yun-Hee ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 193~197
The effect of protein extract from Asterina pectinifera on breast cancer chemopreventive (aromatase and cyclooxygenase-2) and metastatic (matrix metalloproteinase) enzymes was tested. Protein extract from A. pectinifera was capable of suppressing aromatase in a human placenta microsomal assay. Cyclooxygenase-2 (COX-2) activity was significantly inhibited by the protein extract from A. pectinifera at concentrations of 10, 20 and
. The extract markedly reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated matrix metalloproteinase (MMP)-9 activity. These results suggest that A. pectinifera could be of therapeutic value in preventing human breast cancer.
Effect of Low Temperature Vacuum Dried Mackerel Intake on Lipid Profiles and Fatty Acid Compositions in Serum, Heart, Kidney and Adipose Tissue in Mice
Choi, Hyung-Ju ; Kim, Chang-Bok ; Kim, Kyung-Kun ; Lim, Sun-Young ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 198~205
This study was designed to investigate the possibility of the use of dried mackerel, which is enriched with docosahexaenoic (22:6n-3, DHA) and eicosapentaenoic (20:5n-3, EPA) acids, as a means of increasing the intake of these n-3 polyunsaturated fatty acids, and to investigate the effect of the consumption of mackerel on tissue triglyceride, cholesterol contents and fatty acid compositions. Twenty male mice were fed on 5% palm oil (control group) and 5% dried mackerel (mackerel group) diets for four weeks. Total triglyceride, cholesterol and LDL-cholesterol levels tended to decrease in serum, heart and kidney of the mackerel group compared to the control group, while HDL-cholesterol levels were increased with the intake of mackerel. In fatty acid compositions of serum and heart, the mackerel group showed increased percentages of n-3 fatty acids, especially DHA and EPA, and decreased percentages of arachidonic (20:4n-6, AA) and docosapentaenoic (22:5n-6, DPAn-6) acids compared to the control group (p<0.05). There was a similar tendency in kidney and adipose tissue but AA levels were not significantly different between the control and mackerel groups. These results indicate that intake of dried mackerel as the n-3 vehicle for increasing tissue n-3 fatty acids may be associated with improvement in lipid metabolism.
Fatty Acids and Protein Recovery of Squid Viscera with Supercritical Carbon Dioxide
Park, Ji-Yeon ; Back, Sung-Sin ; Chun, Byung-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 206~212
Supercritical carbon dioxide (
) extraction was investigated as a method for protein-sourcing material from squid viscera. To find the optimum conditions, the extraction of squid viscera using
was performed under the conditions of temperature range from 35 to
and constant pressure 25 MPa using Hewlett-Packard 7680T. Also from result of SDS-PAGE, the protein denaturation was minimized when using
extraction. And the major amino acids in the squid viscera were glutamic acid, aspartic acid, lysine, leucine, arginine, alanine, glycine, isoleucine, and valine. The main fatty acids from squid viscera were myristic acid, palmitic acid, stearic acid, heneicosanoic acid, palmitoleic acid, elaidic acid, oleic acid, eicosenoic acid, EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid).
Inhibition of Drug-metabolizing Enzyme and Drug Transporter by Major Components of Phellodendri cortex
Ku, Hei-Young ; Kim, Hyunmi ; Shon, Ji-Hong ; Liu, Kwang-Hyeon ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 213~217
We evaluated the potential of major components of Phellodendri cortex to inhibit the activities of CYP2D6 and p-glycoprotein. The abilities of berberine, palmatine, limonin, and rutaecarpine to inhibit CYP2D6-mediated dextromethorphan O-demethylation and calcein AM accumulation were tested using human liver microsomes and L-MDR1 cell, respectively. Berberine strongly inhibited CYP2D6 isoform activity, whereas limonin and reuaecarpine did not. The
value of berberine was reduced after preincubation with microsomes in the presence of NADPH generating system, suggesting that berberine is a mechanism based inhibitor. In addition, all chemicals tested, didn't show inhibitory effect on p-glycoprotein activity. These results suggest that berberine has potential to inhibit CYP2D6 activity in vitro. Therefore, in vivo studies investigating the interactions between berberine and CYP2D6 substrates are necessary to determine whether inhibition of CYP2D6 activity by berberine is clinically relevant.
Efficacy of Egg Yolk Immunoglobulin (IgY) against Edwardsiella tarda Infection
Kim, Young-Dae ; Oh, Myung-Joo ; Jung, Sung-Ju ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 3, 2006, Pages 218~223
The present study evaluated effect of egg yolk immunoglobulin (IgY) from the hen immunized with Edwardsiella tarda. The purification of anti-E. tarda IgY was performed by polyethylene glycol (PEG). Purified IgY had heavy chain of 64 kDa and light chain of 27 kDa size. The IgY was instable against olive flounders digestive factors and artificially modulated pH 2 and 3. Nevertheless, some activity of IgY appeared in intestine. IgY was orally administered with viable E. tarda to the olive flounders and the efficacy of protection against E. tarda infection was evaluated. Orally administered IgY at a dose of 20 mg/fish delayed infection period of E. tarda cannulated at
to small size (30~40 g) and middle size (110~120 g) flounder. Moreover, orally administered IgY at dose of 20 mg/fish inhibited the penetration of E. tarda cannulated at
into the liver, kidney, spleen and gill via intestine. The fish orally administered with IgY showed increased survival rate. These results suggest that egg yolk containing anti-E. tarda IgY is effective in preventing edwardsiellosis.