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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
Editor in Chief :
Volume & Issues
Volume 1, Issue 4 - Dec 2006
Volume 1, Issue 3 - Sep 2006
Volume 1, Issue 2 - Jun 2006
Volume 1, Issue 1 - Mar 2006
Selecting the target year
Recent Discovery of Bioactive Natural Products from Taiwanese Marine Invertebrates
Shen, Ya-Ching ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 225~231
The secondary metabolites from Taiwanese marine soft corals and sponges have attracted much attention because they possess considerable potential biological activities. To explore the origin of bioactivity, many cytotoxic natural products were isolated and characterized in the past few years. For examples, The lipophilic extracts from marine sponges Petrosia elastica and Ircinia formosana were found active against several human tumor cells. The investigation of the gorgonian Junceela has also resulted in the discovery of a series of new juncenolides. Bioassay-directed fractionation of Clavularia viridis yielded seven new prostanoids. These compounds have been tested and evaluated as potential antitumor agents. The soft corals of the genus Cespitularia produced novel secondary metabolites with diverse chemical structures and interesting biological activities. Four new norditerpenoids, designated cespitulactones and cespihypotins were isolated from Cespitularia hypotentaculata. Cespitulactones are novel structures having a bond cleavage between C-10 and C-11. In addition, three novel diterpenes were isolated from C. taeniata and designated cespitulactams A, B and C having a phenylethyl amino side chain.
Bioactive Compounds Derived from Marine Bacteria: Anti-cancer Activity
Kim, Se-Kwon ; Hoang, Van L.T. ; Kim, Moon-Moo ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 232~242
Bioactive compounds produced by microorganisms have focused on in recent years. In particular, novel compounds showing anti-cancer activity have been isolated from marine microorganisms. In this review, we will discuss on the studies of new bioactive compounds derived from marine bacteria with conjunction to anti-cancer activity. This review will provide an information and source for bioactive compounds showing anti-cancer activity, which were derived from marine bacteria.
Molecular Mechanisms of Cell Cycle Arrest and Apoptosis by Dideoxypetrosynol A, a Polyacetylene from the Sponge Petrosia sp., in Human Monocytic Leukemia Cells
Choi, Yung Hyun ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 243~251
Dideoxypetrosynol A, a polyacetylene from the marine sponge Petrosia sp., is known to exhibit significant selective cytotoxic activity against a small panel of human tumor cell lines, however, the mechanisms of which are poorly understood. In the present study, it was investigated the further possible mechanisms by which dideoxytetrosynol A exerts its anti-proliferative action in cultured human leukemia cell line U937. We observed that the proliferation-inhibitory effect of dideoxypetrosynol A was due to the induction of G1 arrest of the cell cycle and apoptosis, which effects were associated with up-regulation of cyclin D1 and down-regulation of cyclin E without any change in cyclin-dependent-kinases (Cdks) expression. Dideoxypetrosynol A markedly induced the levels of Cdk inhibitor p16/INK4a expression. Furthermore, down-regulation of phosphorylation of retinoblastoma protein (pRB) by this compound was associated with enhanced binding of pRB and the transcription factor E2F-1. The increase in apoptosis was associated with a dose-dependent up-regulation in pro-apoptotic Bax expression and activation of caspase-3 and caspase-9. Dideoxytetrosynol A decreased the levels of cyclooxygenase (COX)-2 mRNA and protein expression without significant changes in the levels of COX-1, which was correlated with a decrease in prostaglandin E2 (PGE2) synthesis. Furthermore, dideoxytetrosynol A treatment markedly inhibited the activity of telomerase, and the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by dideoxytetrosynol A treatment in a dose-dependent fashion. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of dideoxytetrosynol A.
Screening for DPPH Free Radical Scavenging Activities of Autogenous Seaweeds in Jeju Island Using a Electron Spin Resonance (ESR) Spectroscopy
Cha, Seon-Heui ; Heo, Soo-Jin ; Jeon, You-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 252~259
Extracts which were prepared by four different extractions - 80% methanol extracts (ME) at high (
) and a room temperature (
), respectively and aqueous extracts (AE) at both temperatures with the residue after the methanol extracts - of 10 green, 19 brown and 25 red seaweeds collected in Jeju Island coast were examined for their DPPH free radical scavenging activity using a ESR (electron spin resonance) spectroscopy. A variety of the extracts showed positive scavenging effect against DPPH free radical (except the green seaweeds). Among the extracts, the brown seaweed extracts exhibited the highest scavenging activity. Especially, Sargassum spp. of the brown seaweeds have remarkable scavenging activities - both methanolic and aqueous at the both temperatures (
). On the other hand, ME showed better scavenging activity than AE in the red seaweed extracts. These results indicate that autogenous seaweeds in Jeju will be potential natural antioxidants for functional food compounds.
Effect of Solvents and Surfactants on the Whole-cell Bioassay for Screening Quorum Sensing Autoinducers Using the Recombinant Agrobacterium tumefaciens NTL4 Strain
Koh, Kyong-Pyo ; Kim, Yeon-Hee ; Kim, Jung Sun ; Park, Sunghoon ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 260~267
The Liquid Culture Assay using a recombinant Agrobacterium tumefaciens strain has been developed as a means for quorum sensing autoinducer screening. However, the low aqueous solubility of marine natural product extracts used as potential autoinducers has been a hindrance in the screening process. Although the addition of organic solvents and/or surfactants could increase aqueous solubility, errors in data interpretation including false positive results could be a serious problem. Therefore, determining the best possible solvent and surfactant at the optimum concentration is crucial. Evaluating methanol, ethanol, 1-propanol, DMSO and DMF at concentration ranges of 0~10% revealed < 2% methanol to be most favorable when tested for
-gal activity and growth inhibition of the recombinant A. tumefaciens strain. On the other hand, among surfactants tested, Triton X-100 was similarly effective in increasing the delivery of autoinducers for activity at less than 0.05% concentration.
Purification and Characterization of Biosurfactant from Bacillus sp. DYL130
Park, In-Hye ; Kim, Sun-Hee ; Lee, Sang-Cheol ; Ha, Soon-Ok ; Lee, Yong-Seok ; Ryu, Ah-Reum ; Kim, Keun-Ki ; Choi, Yong-Lark ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 268~274
Bacillus sp. DYL130 producing biosurfactant was isolated from soil samples in the Duck-yu mountain and identified as Bacillus sp. by analysis of 16S rDNA sequence. Purification of the biosurfactant was performed by using affinity chromatography and TLC. The biosurfactant of culture medium from Bacillus sp. DYL130 was eluted with 100% methanol using affinity chromatography. To remove methanol, a rotary evaporator was used and enrichment sample was dissolved in alkaline water(pH 10). The purified biosurfactant was identified by TLC. It was confirmed that the Rf value of the biosurfactant was 0.78. Antifungal activity against Botrytis cineria was showed the strongly activity as active antagonist. Maximum emulsification activity and stability were obtained from soybean oil. The critical micelle concentration (CMC) of purified biosurfactant was 35mg/l and the purified biosurfactant inhibited biofilm forming by Bacillus sp..
Isolation and Characterization of 3,4-Dichloroaniline Degrading Bacteria from a Sandbank
Kim, Young-Mog ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 275~281
The compound 3,4-dichloroaniline (DCA) is an aromatic amine used as an intermediate product in the synthesis of herbicides, azo-dyes and harmaceuticals. It is also a degradation product of some herbicides (diuron, propanil, and linuron) and of trichlorocarbanilide, a chemical used as active agent in the cosmetic industry. 3,4-DCA, however, is considered potential pollutants due to their toxic and recalcitrant properties to humans and other species. A bacterium capable of growth on 3,4-DCA was isolated by dilution method from 3,4-DCA-containing enrichment culture. Finally, a strain, YM-14, capable of degrading efficiently 3,4-DCA was isolated from a sandbank. The isolated strain, YM-14 was identified to be Arthrobacter sp.. Fifty ppm 3,4-DCA in 1/10 LB media was completely degraded by the growth of Arthrobacter sp. YM-14 for 12 h at
. The isolated strain is capable of growth on 3,4-DCA as sole carbon source and also able to degrade other chloroaniline compounds. Also, the isolated strain showed high level of catechol 1,2-dioxygenase activity by 3,4-DCA exposure. The catechol 1,2-dioxygenase was supposed to be ones of the important factors for 3,4-DCA degradation.
Effects of the Molecular Weight and Type of Chitosans on Shelf-life of Makkulli
Shin, A-Ga ; Jung, Yoo-Kyung ; Lee, Ye-Kyung ; Kang, Meung-Soo ; No, Hong-Kyoon ; Kim, Soon-Dong ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 282~291
Effects of the molecular weight and type of chitosans on shelf-life of Makkulli were evaluated during 18 days of storage at
. Two types of chitosans were studied:
-chitosans with 11 different molecular weights (water-soluble, Mw = 1, 8, 22, 43, 67 and 616 kDa; acid-soluble, Mw = 282, 440, 746, 1,110 and 2,025 kDa) and
-chitosan (acid-soluble, Mw = 577 kDa). Acid-soluble chitosans were applied as a form of chitosan-ascorbate. All chitosans were added to Makkulli at 0.002% concentration, the optimum concentration established in a preliminary test. Among 12 chitosans, the
-chitosans with 22 and 440 kDa exhibited stronger antimicrobial effects than did other
-chitosans. The results for pH, acidity, alcohol concentration, viable cell counts, and sensory evaluation suggested that addition of
-chitosans with 22 and 440 kDa increased the shelf-life of Makkulli by almost 1 week at
compared with that of control (without chitosan) and other chitosan-added groups. Extension of Makkulli shelf-life by 1 week is fairly significant in view of the magnitude of the total amount of Makkulli produced in Korea.
Highly Pure Culture of Photosynthetic Bacteria for Pilot-scale Production
Cho, Kyoung Sook ; Yim, Tae Bin ; Jeong, Hae Yoon ; Cho, Jeong Sub ; Kim, Joong Kyun ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 292~297
Lab-scale and pilot-scale productions of photosynthetic bacteria, that were able to efficiently treat wastewater from aquacultural farm, were experimented for their highly-pure culture. The results of experiments in a gas pack reactor, an anaerobic flask and a flask using
gas as N-source showed that only photosynthetic bacteria formed red colonies on agar plate and their purity was over 90% in a colony, observed under a microscope. It was found that the basal medium could most promote the growth of photosynthetic bacteria, confirmed by experiments of serial cultures on various media. Under the culture conditions, the specific growth rate was found to be
from the culture in 5L bioreactor and the same value could be obtained in pilot-scale production.
Immunochemical Studies of Starfish Gangliosides: Production of Monoclonal Antibody against AG-2, the Major Ganglioside of Starfish Acanthaster planci, and Detecting Its Distribution in Tissues by TLC Immunostaining
Miyamoto, Tomofumi ; Yamamoto, Atsushi ; Sakai, Maki ; Tanaka, Hiroyuki ; Shoyama, Yukihiro ; Higuchi, Ryuichi ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 298~304
In this study, we establish a thin-layer chromatography (TLC) immunostaining method for detecting starfish gangliosides. A new monoclonal antibody (MAb) against AG-2, the major gangliosides molecular species of Acanthaster planci, was produced by fusing hybridoma with splenocytes immunized to liposomal AG-2. BALB/c male mice were injected with liposomal AG-2 antigen, and immunized. Their splenocytos were isolated and fused with hypoxanthine-aminopterine-thimidine (HAT)-sensitive mouse myeloma cells. Hybridomas producing MAb reactive to AG-2 were cloned using the limited dilution method. Established hybridomas were cultured in eRDF medium. Crude MAb produced from clone 8D4 was purified with a magnesium pyrophosphate column. Enzyme immunoassay and TLC immunostaining of AG-2 were performed using the purified MAb. Structurally related gangliosides did not cross-react with anti-AG-2 antibodies. The detection limit of TLC immunostaining was 50 ng of AG-2. The newly established immunostaining method was further developed for detecting AG-2 distribution and qualitative analysis in tissues and/or organs. Our results show that the majority of AG-2 is present in the stomach of male A. planci, while AG-2 is distributed not only in the stomach but also in the the pyloric caeca of female A. planci.
Inhibition of Wnt/
-Catenin Pathway by Dictyota dichotoma Extract
Cho, Munju ; Oh, Sangtaek ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 305~310
Abnormal activation of the Wnt/
-catenin pathway and subsequent up-regulation of
-catenin response transcription (CRT) are associated with the development of colon cancer. Thus, the Wnt/
-catenin pathway is an attractive target for chemoprevention and treatment of this cancer. In this study, we used a cell-based screen to identify a methanol extract of Dictyota dichotoma (EDD) that suppresses the Wnt/
-catenin pathway without altering the level of
-catenin protein and reduces the expression of cyclin D1, which is a known
-catenin/T cell factor (TCF)-dependent gene. EDD inhibited the growth of various colon cancer cells. Our findings suggest that EDD can potentially be used as a chemopreventive agent against colon cancer.
Fish Oil Variation during Enzymatic Ethanolysis
Shin, Sang-Kyu ; Yoo, Hong-Suk ; Pack, Hyun-Duk ; Chun, Byung-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 1, issue 4, 2006, Pages 311~316
Enzymatic ethanolysis of fish oil with immobilized lipase was investigated for reducing the free fatty acid contents and enhancing the function of fish oil. Ethanolysis reactions were carried out in erlenmeyer flask (25ml) containing a mixture of squid viscera oil and 99.9% ethanol using 1% (based on w/w squid viscera oil) immobilized lipase. The reaction mixtures were incubated at
and shaken at 100rpm. Ethanol was added into the mixture by stepwise addition method of Shinmada. Measurement of free fatty acid molar amounts was studied by Acid Value. Tendency of oil variation during transesterification was studied by TLC method. Enzymatic ethanolysis composed diglyceride, monoglyceride and fatty acid ethyl ester with reducing free fatty acid contents. Also, selective ethanolysis by Lipozyme TL-IM and Lipozyme RM-IM mostly did not react at the sn-2 position of squid viscera oil. Lipozyme RM-IM was more suitable enzyme to reduce the free fatty acid contents by ethanolysis than Lipozyme TL-IM. Squid viscera oil was transformed into suitable properties (5 in Acid Value) for functional fish oil production.